LOCUS       Exported               10120 bp ds-DNA     circular SYN 16-JUN-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10120)
  AUTHORS   Leonard Lab
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Jan 12, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10120
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    340..575
                     /label=cHS4
     misc_feature    679..914
                     /label=cHS4
     misc_feature    1031..1068
                     /label=attB BxB1
     CDS             1079..1678
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    1856..1911
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_feature    2266..2501
                     /label=cHS4
     misc_feature    2605..2840
                     /label=cHS4
     misc_feature    2929..2953
                     /label=TUPV1 Barcode
     promoter        3010..3034
                     /label=YB_TATA Minimal Promoter
                     /note="From Hansen et al., PNAS 2014"
     regulatory      3064..3073
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
     CDS             3070..3768
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    3819..4043
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    4061..4085
                     /label=TUPV1 Barcode
     misc_feature    4109..4344
                     /label=cHS4
     misc_feature    4448..4683
                     /label=cHS4
     misc_feature    4773..4797
                     /label=TUPV2 Barcode
     promoter        5093..6274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     intron          5323..6265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human
                     EF-1-alpha"
     regulatory      6375..6384
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
     CDS             6381..7097
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     CDS             7125..7181
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7194..7592
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    7643..7867
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    7885..7909
                     /label=TUPV2 Barcode
     misc_feature    7933..8168
                     /label=cHS4
     misc_feature    8272..8507
                     /label=cHS4
     CDS             complement(8726..9535)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      complement(join(9694..10120,1..162))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
       61 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      121 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      181 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      241 ttatcaaaaa ggatcttcac ctagatcctt tctcgagctt tcggagtgag atggagtggc
      301 cactgtggaa ctgggcctag catcgcgata agctctagag ggacagcccc cccccaaagc
      361 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
      421 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
      481 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
      541 tgcagacacc tggggggata cggggaaaaa gctttaggct gaaagagaga tttagaatga
      601 cagaatcata gaacggcctg cgtacggatg gggctctagc tctagagctt gatatcgaat
      661 tcctgcagcc ccgctagagg gacagccccc ccccaaagcc cccagggatg taattacgtc
      721 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
      781 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
      841 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
      901 ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag aacggcctgg
      961 gcgcgcctgg tggcgaacga tcaacagacg ggagatctac tattgggaca gcagagatcc
     1021 agttggtaat ggcttgtcga cgacggcggt ctccgtcgtc aggatcatag gtgccaccat
     1081 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     1141 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     1201 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     1261 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     1321 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     1381 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     1441 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     1501 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     1561 gacatccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     1621 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagc
     1681 ttccactgga ttgtacaatt accaactgaa ttcactcctc aggtgcaggc tgcctatcag
     1741 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     1801 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     1861 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     1921 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     1981 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     2041 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     2101 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     2161 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     2221 cttctcttat ggagatccgg gcctagcatc gcgataagct ctagagggac agcccccccc
     2281 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     2341 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     2401 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     2461 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaaa gagagattta
     2521 gaatgacaga atcatagaac ggcctgcgta cggatggggc tctagctcta gagcttgata
     2581 tcgaattcct gcagccccgc tagagggaca gccccccccc aaagccccca gggatgtaat
     2641 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
     2701 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
     2761 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
     2821 ggatacgggg aaaaagcttt aggctgaaag agagatttag aatgacagaa tcatagaacg
     2881 gcctgggcgc gcctggtggc gaacgatcaa cagacgggag attgccaagg tcttgacaaa
     2941 cgagtgctag tacaagatcg cgtcgacgga tcgggagatc tgaattcgag accacaggtc
     3001 tcggatccct ctagagggta tataatgggg gccactagtc tactaccaga gctcatcgct
     3061 agcgccacca tggtgagcga gctgattaag gagaacatgc acatgaagct gtatatggag
     3121 ggcaccgtga acaaccacca cttcaagtgt acctccgagg gcgaaggcaa gccctacgag
     3181 ggcacccaga ccatgagaat caaggcggtc gagggcggcc ctctcccctt cgccttcgac
     3241 atcctggcta ccagcttcat gtacggcagc aaaaccttca tcaaccacac ccagggcatc
     3301 cccgacttct ttaagcagtc cttccccgag ggcttcacat gggagagagt caccacatac
     3361 gaagatgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
     3421 tacaacgtca agatcagagg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa
     3481 acactcggct gggaggcctc caccgaaacc ctgtaccccg ctgacggcgg cctggaaggc
     3541 agagccgaca tggccctgaa gctcgtgggc gggggccacc tgatctgcaa cttgaaaacc
     3601 acatacagat ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac
     3661 aggagactgg aaagaatcaa ggaggccgac aaagaaacct acgtcgagca gcacgaggtg
     3721 gctgtggcca gatactgcga cctccctagc aaactggggc acagatgagc ggccgctcga
     3781 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     3841 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     3901 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     3961 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagtcaata gcaggcatgc
     4021 tggggatgcg gtgggctcta tggcttctga ggcaggtcga gggtacatgc gccttactcc
     4081 ttgtgagcgc atcgcgataa gctctagagg gacagccccc ccccaaagcc cccagggatg
     4141 taattacgtc cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc
     4201 cggcgctccc cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag
     4261 gtggcacggg atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct
     4321 ggggggatac ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag
     4381 aacggcctgc gtacggatgg ggctctagct ctagagcttg atatcgaatt cctgcagccc
     4441 cgctagaggg acagcccccc cccaaagccc ccagggatgt aattacgtcc ctcccccgct
     4501 agggggcagc agcgagccgc ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc
     4561 gagccggcag cgtgcgggga cagcccgggc acggggaagg tggcacggga tcgctttcct
     4621 ctgaacgctt ctcgctgctc tttgagcctg cagacacctg gggggatacg gggaaaaagc
     4681 tttaggctga aagagagatt tagaatgaca gaatcataga acggcctggg cgcgcctggt
     4741 ggcgaacgat caacagacgg gagagggcaa aaccgatgtt gacggactaa tcctgacaag
     4801 atctggggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     4861 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
     4921 acgggccaga tatacgcgtg agtaattcat acaaaaggac tcgcccctgc cttggggaat
     4981 cccagggacc gtcgttaaac tcccactaac gtagaaccca gagatcgctg cgttcccgcc
     5041 ccctcacccg cccgctctcg tcatcactga ggtggagaag agcatgcgtg aggctccggt
     5101 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     5161 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     5221 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     5281 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt
     5341 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacg
     5401 cccctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag
     5461 ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcttgg
     5521 gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga
     5581 taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga
     5641 tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg
     5701 cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg
     5761 gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct
     5821 cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc
     5881 gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg
     5941 gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc
     6001 agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt
     6061 ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt
     6121 tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc
     6181 cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt
     6241 tcaaagtttt tttcttccat ttcaggtgtc gtgagagctc gtttagtgaa ccgtcagatc
     6301 gcctggagac gccatccacg ctgttttgac ctccatagaa gagaacggga ccgatccaaa
     6361 ttctcgaggc tagcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     6421 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg aggggcgagg
     6481 gcgagggcga tgccaccaac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     6541 tgcccgtgcc ctggcccacc ctcgtgacca ccctgagcca cggcgtgcag tgcttcgccc
     6601 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     6661 tccaggagcg caccatcttc ttcaaggacg acggcaccta caagacccgc gccgaggtga
     6721 agttcgaggg cgacacccta gtgaaccgca tcgagctgaa gggcgtcgac ttcaaggagg
     6781 acggcaacat cctggggcac aagctggagt acaacttcaa cagccacaac atctatatca
     6841 tggccgtcaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacgtggagg
     6901 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     6961 tgctgctgcc cgacagccac tacctgagca cccagtccgt gctgagcaaa gaccccaacg
     7021 agaagcgcga tcacatggtc ctgctggagt tccgcaccgc cgccgggatc actctcggca
     7081 tggacgagct gtacaaggaa ttcggtagca agcttggtag tggagcaact aacttttccc
     7141 tgctcaaaca agccggggat gtagaggaga accccggccc cgcccccctt aagatggcca
     7201 agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca
     7261 tccccatctc tgaggactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct
     7321 tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg
     7381 gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga
     7441 acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg
     7501 ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat
     7561 tgctgccctc tggttatgtg tgggagggct aagcggccgc tcgagtctag agggcccgtt
     7621 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     7681 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     7741 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     7801 caggacagca agggggagga ttgggaagtc aatagcaggc atgctgggga tgcggtgggc
     7861 tctatggctt ctgaggcagg tcgaagaact atttcctggc tgttacgcga gcgcatcgcg
     7921 ataagctcta gagggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc
     7981 ccgctagggg gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca
     8041 tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct
     8101 ttcctctgaa cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa
     8161 aaagctttag gctgaaagag agatttagaa tgacagaatc atagaacggc ctgcgtacgg
     8221 atggggctct agctctagag cttgatatcg aattcctgca gccccgctag agggacagcc
     8281 cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg cagcagcgag
     8341 ccgcccgggg ctccgctccg gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg
     8401 gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac gcttctcgct
     8461 gctctttgag cctgcagaca cctgggggga tacggggaaa aagctttagg ctgaaagaga
     8521 gatttagaat gacagaatca tagaacggcc tgggcgcgcc tggtggcgaa cgatcaacag
     8581 acgggagagg actagaggat gcacatgtga ccgagggatg gaacgacgcg taactcacgt
     8641 taagggattt tggtcatgag cttgcgccgt cccgtcaagt cagcgtaatg ctctgccagt
     8701 gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa tgaaactgca
     8761 atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag
     8821 gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc
     8881 cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa
     8941 gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt
     9001 ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa
     9061 ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa
     9121 aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa
     9181 caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt tttccgggga
     9241 tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa
     9301 gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa
     9361 cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaagcgat
     9421 agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag
     9481 catccatgtt ggaatttaat cgcggcctcg acgtttcccg ttgaatatgg ctcataacac
     9541 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat attattttat
     9601 cttgtgcaat gtaacatcag agattttgag acacgggcca tgctagctcc agcaaaaggc
     9661 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     9721 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     9781 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     9841 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     9901 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     9961 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    10021 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    10081 aggcggtgct acagagttct tgaagtggtg gcctaactac
//
