LOCUS       Exported                4715 bp ds-DNA     circular SYN 07-APR-2018
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Leonard Lab
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jan 10, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    31..39
                     /label=ZF43
                     /note="Binding site for ZF43-8 (Khalil, et al. Cell 2012)"
     misc_feature    56..64
                     /label=ZF43
                     /note="Binding site for ZF43-8 (Khalil, et al. Cell 2012)"
     misc_feature    87..95
                     /label=ZF43
                     /note="Binding site for ZF43-8 (Khalil, et al. Cell 2012)"
     misc_feature    112..120
                     /label=ZF43
                     /note="Binding site for ZF43-8 (Khalil, et al. Cell 2012)"
     misc_feature    143..151
                     /label=ZF43
                     /note="Binding site for ZF43-8 (Khalil, et al. Cell 2012)"
     misc_feature    168..176
                     /label=ZF43
                     /note="Binding site for ZF43-8 (Khalil, et al. Cell 2012)"
     misc_feature    215..223
                     /label=ZF43
                     /note="Binding site for ZF43-8 (Khalil, et al. Cell 2012)"
     misc_feature    240..248
                     /label=ZF43
                     /note="Binding site for ZF43-8 (Khalil, et al. Cell 2012)"
     misc_feature    271..279
                     /label=ZF43
                     /note="Binding site for ZF43-8 (Khalil, et al. Cell 2012)"
     misc_feature    296..304
                     /label=ZF43
                     /note="Binding site for ZF43-8 (Khalil, et al. Cell 2012)"
     misc_feature    327..335
                     /label=ZF43
                     /note="Binding site for ZF43-8 (Khalil, et al. Cell 2012)"
     misc_feature    352..360
                     /label=ZF43
                     /note="Binding site for ZF43-8 (Khalil, et al. Cell 2012)"
     promoter        524..548
                     /label=YB_TATA Minimal Promoter
                     /note="From Hansen et al., PNAS 2014"
     CDS             584..1303
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    1354..1578
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1624..2052
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      2122..2257
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(2963..3548)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3719..4579)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4580..4684)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctgaa ttcggatcca gagtgaggac tacctataga agtaagagtg
       61 aggacaatcg tatacaagta gatccagagt gaggactacc tatagaagta agagtgagga
      121 caatcgtata caagtagatc cagagtgagg actacctata gaagtaagag tgaggacaat
      181 cgtatacaag tagatccctc tagagggaga tccagagtga ggactaccta tagaagtaag
      241 agtgaggaca atcgtataca agtagatcca gagtgaggac tacctataga agtaagagtg
      301 aggacaatcg tatacaagta gatccagagt gaggactacc tatagaagta agagtgagga
      361 caatcgtata caagtagatc cctctagagg gagatcctga gtagtgcgcg agcaaaattt
      421 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
      481 gttttgcgct gcttcgcgat gtacgggcca gatatacgat ccctctagag ggtatataat
      541 gggggccact agtctactac cagagctcat cgctagcgcc accatggtga gcaagggcga
      601 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      661 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      721 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg
      781 ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
      841 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
      901 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
      961 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     1021 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     1081 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     1141 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc
     1201 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     1261 cgccgccggg atcactctcg gcatggacga gctgtacaag taggcggccg ctcgagtcta
     1321 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     1381 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     1441 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     1501 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     1561 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     1621 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     1681 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     1741 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     1801 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     1861 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     1921 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     1981 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     2041 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggc tcaattagtc agcaaccata
     2101 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     2161 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     2221 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     2281 ggagcttgta tatccatttt cggaggttgg gcttcggaat cgttttccgg gacgccggct
     2341 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     2401 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     2461 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     2521 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     2581 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     2641 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     2701 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     2761 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2821 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2881 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2941 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3001 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3061 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3121 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3181 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3241 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3301 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3361 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3421 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3481 aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3541 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3601 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3661 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     3721 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     3781 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     3841 gtgctgcaat gataccgcga gatccacgct caccggctcc agatttatca gcaataaacc
     3901 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     3961 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     4021 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     4081 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     4141 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     4201 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     4261 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     4321 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     4381 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     4441 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     4501 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     4561 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     4621 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     4681 cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
