LOCUS       Exported                4261 bp ds-DNA     circular SYN 07-APR-2018
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   LeonardLab
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jan 10, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    39..47
                     /label=ZF43
                     /note="Binding site for ZF43-8 (Khalil, et al. Cell 2012)"
     misc_feature    54..62
                     /label=ZF43
                     /note="Binding site for ZF43-8 (Khalil, et al. Cell 2012)"
     misc_feature    69..77
                     /label=ZF43
                     /note="Binding site for ZF43-8 (Khalil, et al. Cell 2012)"
     promoter        85..109
                     /label=YB_TATA Minimal Promoter
                     /note="From Hansen et al., PNAS 2014"
     CDS             145..864
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    915..1139
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1185..1613
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      1683..1818
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(2524..3109)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3280..4140)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4141..4245)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtgccacctg acgtcgacgg atcgggagat ctgaattaga gtgaggacga tcggagtgag
       61 gataattaga gtgaggacga tccctctaga gggtatataa tgggggccac tagtctacta
      121 ccagagctca tcgctagcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
      181 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      241 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      301 aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct gcagtgcttc
      361 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      421 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      481 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      541 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
      601 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
      661 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
      721 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc
      781 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
      841 ggcatggacg agctgtacaa gtaggcggcc gctcgagtct agagggcccg tttaaacccg
      901 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
      961 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     1021 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     1081 caagggggag gattgggaag tcaatagcag gcatgctggg gatgcggtgg gctctatggc
     1141 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     1201 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     1261 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     1321 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     1381 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     1441 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     1501 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     1561 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     1621 tggaatgtgt gtcagttagg ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     1681 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     1741 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     1801 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     1861 tcggaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     1921 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     1981 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     2041 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     2101 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     2161 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     2221 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     2281 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     2341 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2401 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2461 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2521 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2581 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2641 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2701 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2761 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2821 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2881 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2941 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3001 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3061 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3121 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3181 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3241 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3301 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3361 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3421 agatccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3481 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3541 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3601 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3661 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3721 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3781 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3841 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3901 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3961 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     4021 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     4081 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     4141 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     4201 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     4261 a
//
