LOCUS       Exported                4364 bp ds-DNA     circular SYN 07-APR-2018
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   LeonardLab
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jan 10, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    67..75
                     /label=ZF43
                     /note="Binding site for ZF43-8 (Khalil, et al. Cell 2012)"
     misc_feature    82..90
                     /label=ZF43
                     /note="Binding site for ZF43-8 (Khalil, et al. Cell 2012)"
     misc_feature    97..105
                     /label=ZF43
                     /note="Binding site for ZF43-8 (Khalil, et al. Cell 2012)"
     misc_feature    112..120
                     /label=ZF43
                     /note="Binding site for ZF43-8 (Khalil, et al. Cell 2012)"
     misc_feature    127..135
                     /label=ZF43
                     /note="Binding site for ZF43-8 (Khalil, et al. Cell 2012)"
     misc_feature    142..150
                     /label=ZF43
                     /note="Binding site for ZF43-8 (Khalil, et al. Cell 2012)"
     misc_feature    157..165
                     /label=ZF43
                     /note="Binding site for ZF43-8 (Khalil, et al. Cell 2012)"
     misc_feature    172..180
                     /label=ZF43
                     /note="Binding site for ZF43-8 (Khalil, et al. Cell 2012)"
     promoter        188..212
                     /label=YB_TATA Minimal Promoter
                     /note="From Hansen et al., PNAS 2014"
     CDS             248..967
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    1018..1242
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1288..1716
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      1786..1921
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(2627..3212)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3383..4243)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4244..4348)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtgccacctg acgtcgacgg atcgggagat ctgaatttga gaccacagtg atacggtctc
       61 cgatcggagt gaggaaaatt agagtgagga cgatcggagt gaggataatt agagtgagga
      121 cgatcggagt gaggaaaatt agagtgagga cgatcggagt gaggataatt agagtgagga
      181 cgatccctct agagggtata taatgggggc cactagtcta ctaccagagc tcatcgctag
      241 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
      301 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      361 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
      421 gcccaccctc gtgaccacct tcggctacgg cctgcagtgc ttcgcccgct accccgacca
      481 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      541 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      601 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      661 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
      721 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
      781 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
      841 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca
      901 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
      961 caagtaggcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg
     1021 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     1081 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     1141 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     1201 aagtcaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     1261 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     1321 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     1381 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     1441 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     1501 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     1561 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     1621 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     1681 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     1741 aggctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     1801 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     1861 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     1921 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggagg ttgggcttcg
     1981 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     2041 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     2101 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     2161 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     2221 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     2281 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     2341 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     2401 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     2461 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     2521 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     2581 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2641 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2701 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2761 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2821 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2881 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2941 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3001 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3061 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3121 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc
     3181 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3241 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3301 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3361 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     3421 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     3481 gggagggctt accatctggc cccagtgctg caatgatacc gcgagatcca cgctcaccgg
     3541 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     3601 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3661 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     3721 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     3781 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     3841 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     3901 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     3961 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     4021 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     4081 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     4141 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     4201 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     4261 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     4321 agaaaaataa acaaataggg gttccgcgca catttccccg aaaa
//
