LOCUS       Exported                4330 bp ds-DNA     circular SYN 19-APR-2018
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Leonard Lab
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jan 10, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    63..71
                     /label=ZF158
                     /note="Binding site for ZF158-2 (Khalil, et al. Cell 2012)"
     misc_feature    78..86
                     /label=ZF158
                     /note="Binding site for ZF158-2 (Khalil, et al. Cell 2012)"
     misc_feature    93..101
                     /label=ZF158
                     /note="Binding site for ZF158-2 (Khalil, et al. Cell 2012)"
     misc_feature    108..116
                     /label=ZF158
                     /note="Binding site for ZF158-2 (Khalil, et al. Cell 2012)"
     misc_feature    123..131
                     /label=ZF158
                     /note="Binding site for ZF158-2 (Khalil, et al. Cell 2012)"
     misc_feature    138..146
                     /label=ZF158
                     /note="Binding site for ZF158-2 (Khalil, et al. Cell 2012)"
     promoter        154..178
                     /label=YB_TATA Minimal Promoter
                     /note="From Hansen et al., PNAS 2014"
     CDS             214..933
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    984..1208
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1254..1682
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      1752..1887
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(2593..3178)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3349..4209)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4210..4314)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtgccacctg acgtcgacgg atcgggagat ctgcgagtca gtgcagacgg caagtggtcg
       61 atgtagatgg agacgctgta gatggaggat ctgtagatgg aggctttgta gatggagagc
      121 ctgtagatgg aggccgtgta gatggagacg ctctctagag ggtatataat gggggccact
      181 agtctactac cagagctcat cgctagcgcc accatggtga gcaagggcga ggagctgttc
      241 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      301 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      361 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg
      421 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      481 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      541 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      601 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
      661 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
      721 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      781 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc
      841 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      901 atcactctcg gcatggacga gctgtacaag taggcggccg ctcgagtcta gagggcccgt
      961 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     1021 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     1081 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     1141 gcaggacagc aagggggagg attgggaagt caatagcagg catgctgggg atgcggtggg
     1201 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     1261 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     1321 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     1381 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     1441 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     1501 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     1561 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     1621 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     1681 ttaattctgt ggaatgtgtg tcagttaggc tcaattagtc agcaaccata gtcccgcccc
     1741 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     1801 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     1861 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     1921 tatccatttt cggaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     1981 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     2041 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     2101 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     2161 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     2221 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     2281 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     2341 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     2401 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2461 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2521 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2581 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2641 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2701 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2761 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2821 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2881 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2941 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3001 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     3061 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3121 tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3181 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3241 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3301 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     3361 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     3421 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     3481 gataccgcga gatccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     3541 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     3601 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     3661 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     3721 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     3781 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     3841 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     3901 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     3961 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     4021 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     4081 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     4141 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4201 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     4261 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     4321 tccccgaaaa
//
