LOCUS       Exported               13025 bp ds-DNA     circular SYN 15-JAN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 15, 2020 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13025
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             828..1016
                     /label=5' LTR
                     /note="5' LTR (R and U5 portions; U3 was replaced by the 
                     CMV promoter)"
     primer_bind     complement(986..1016)
                     /label=Primer 4
     misc_feature    1067..1204
                     /label=Psi
                     /note="Psi"
     misc_feature    1124..1127
                     /label=SD; splice donor
                     /note="SD; splice donor"
     misc_feature    1171..1535
                     /label=gag
                     /note="gag"
     primer_bind     complement(1356..1379)
                     /label=Primer 6
     misc_feature    1681..1922
                     /label=RRE
                     /note="RRE"
     primer_bind     complement(1955..1977)
                     /label=Primer 5
     misc_feature    2440..2557
                     /label=CPPT; central polypurine tract
                     /note="CPPT; central polypurine tract"
     promoter        2608..2857
                     /label=Human U6
     primer_bind     2608..2632
                     /label=Primer 7
     primer_bind     2608..2629
                     /label=Primer 9
     primer_bind     2782..2801
                     /label=Primer 12
     misc_feature    2873..2965
                     /label=sgRNA F+E
     misc_feature    2937..3008
                     /label=Untitled
     primer_bind     2937..2956
                     /label=Primer 10
     misc_feature    2943..3002
                     /label=barcode oligo
                     /note="barcode oligo"
     primer_bind     complement(2946..2958)
                     /label=Primer 10
     promoter        2984..3239
                     /label=EFS-NS
                     /note="EFS-NS"
     primer_bind     2984..3009
                     /label=Primer 1
     primer_bind     complement(2990..3009)
                     /label=Primer 11
     primer_bind     complement(3195..3217)
                     /label=Primer 8
                     /note="LentiCRISPR V2 (LCV2) PCR1 R"
     primer_bind     complement(3219..3239)
                     /label=Primer 2
     misc_feature    3263..7366
                     /label=xCas9
                     /note="xCas9"
     primer_bind     3711..3730
                     /label=Primer 13
     primer_bind     4029..4058
                     /label=Primer 15
     primer_bind     complement(4036..4065)
                     /label=Primer 14
     misc_feature    4046..4048
                     /label=A262T
                     /note="A262T"
     primer_bind     4161..4180
                     /label=Primer 20
     primer_bind     4215..4244
                     /label=Primer 17
     primer_bind     complement(4222..4251)
                     /label=Primer 16
     misc_feature    4232..4234
                     /label=R324L
                     /note="R324L"
     primer_bind     4470..4499
                     /label=Primer 19
     primer_bind     complement(4477..4506)
                     /label=Primer 18
     misc_feature    4487..4489
                     /label=S409I
                     /note="S409I"
     primer_bind     4630..4649
                     /label=Primer 25
     primer_bind     4686..4712
                     /label=Primer 24
     primer_bind     complement(4690..4719)
                     /label=Primer 21
     misc_feature    4700..4702
                     /label=E480K
                     /note="E480K"
     primer_bind     4872..4901
                     /label=Primer 23
     primer_bind     complement(4879..4908)
                     /label=Primer 22
     misc_feature    4889..4891
                     /label=E543D
                     /note="E543D"
     primer_bind     5087..5106
                     /label=Primer 27
     primer_bind     complement(5131..5150)
                     /label=Primer 26
     primer_bind     5151..5170
                     /label=Primer 30
     primer_bind     5325..5354
                     /label=Primer 29
     primer_bind     complement(5332..5361)
                     /label=Primer 28
     misc_feature    5342..5344
                     /label=M694I
                     /note="M694I"
     primer_bind     5670..5689
                     /label=Primer 33
     primer_bind     5762..5791
                     /label=Primer 32
     primer_bind     complement(5770..5799)
                     /label=Primer 31
     primer_bind     6192..6211
                     /label=Primer 34
     primer_bind     6719..6738
                     /label=Primer 37
     primer_bind     6900..6929
                     /label=Primer 36
     primer_bind     complement(6907..6936)
                     /label=Primer 35
     misc_feature    6917..6919
                     /label=E1219V
                     /note="E1219V"
     primer_bind     7164..7183
                     /label=Primer 38
     misc_feature    7367..7414
                     /label=NLS
                     /note="NLS"
     misc_feature    7415..7438
                     /label=FLAG
                     /note="FLAG"
     misc_feature    7439..7504
                     /label=P2A
                     /note="P2A"
     CDS             7505..8101
                     /label=Puro
                     /note="Puro"
     primer_bind     7505..7526
                     /label=Primer 3
     misc_binding    8117..8705
                     /label=WPRE
                     /note="WPRE"
     LTR             8776..9011
                     /label=3' LTR SIN
     primer_bind     complement(8981..9011)
                     /label=Primer 4
     rep_origin      11460..11685
                     /direction=RIGHT
                     /label=ColE1
                     /note="ColE1"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg
     2641 catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag
     2701 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     2761 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     2821 ttggctttat atatcttgtg gaaaggacga aacaccggag acggacgtct ctgtttaaga
     2881 gctatgctgg aaacagcata gcaagtttaa ataaggctag tccgttatca acttgaaaaa
     2941 gtggcaccga gtcggtgctt tttttgaatt cttgtgggct agctaggtct tgaaaggagt
     3001 gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     3061 gttgggggga ggggtcggca attgatccgg tgcctagaga aggtggcgcg gggtaaactg
     3121 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     3181 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagga
     3241 ccggttctag agcgctgcca ccatggacaa gaagtacagc atcggcctgg acatcggcac
     3301 caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa
     3361 ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag ccctgctgtt
     3421 cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa gaagatacac
     3481 cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt
     3541 ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca
     3601 cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc
     3661 caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg acctgcggct
     3721 gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga
     3781 cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc agacctacaa
     3841 ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc
     3901 tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc ccggcgagaa
     3961 gaagaatggc ctgttcggaa acctgattgc cctgagcctg ggcctgaccc ccaacttcaa
     4021 gagcaacttc gacctggccg aggataccaa actgcagctg agcaaggaca cctacgacga
     4081 cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt ttctggccgc
     4141 caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca ccgagatcac
     4201 caaggccccc ctgagcgcct ctatgatcaa gctgtacgac gagcaccacc aggacctgac
     4261 cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga ttttcttcga
     4321 ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg aagagttcta
     4381 caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc tcgtgaagct
     4441 gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcatca tcccccacca
     4501 gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt acccattcct
     4561 gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct actacgtggg
     4621 ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg aggaaaccat
     4681 caccccctgg aacttcgaga aggtggtgga caagggcgct tccgcccaga gcttcatcga
     4741 gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca agcacagcct
     4801 gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg
     4861 aatgagaaag cccgccttcc tgagcggcga ccagaaaaag gccatcgtgg acctgctgtt
     4921 caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga
     4981 gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac
     5041 ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga
     5101 ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga
     5161 ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg
     5221 gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa
     5281 gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt
     5341 catccagctg atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt
     5401 gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat
     5461 taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg
     5521 gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca cccagaaggg
     5581 acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag
     5641 ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct
     5701 gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct
     5761 gtccgactac gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg actccatcga
     5821 caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga
     5881 agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac
     5941 ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa
     6001 ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca
     6061 gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt
     6121 gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta
     6181 caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt
     6241 gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta
     6301 caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac
     6361 cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc
     6421 caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt
     6481 gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa
     6541 tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa
     6601 gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg
     6661 cttcgacagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa
     6721 gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag
     6781 cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga
     6841 cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat
     6901 gctggcctct gccggcgtgc tgcagaaggg aaacgaactg gccctgccct ccaaatatgt
     6961 gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga
     7021 gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat
     7081 cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc
     7141 ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt
     7201 taccctgacc aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg
     7261 gaagaggtac accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac
     7321 cggcctgtac gagacacgga tcgacctgtc tcagctggga ggcgacaagc gacctgccgc
     7381 cacaaagaag gctggacagg ctaagaagaa gaaagattac aaagacgatg acgataaggg
     7441 atccggcgca acaaacttct ctctgctgaa acaagccgga gatgtcgaag agaatcctgg
     7501 accgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     7561 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     7621 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     7681 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     7741 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     7801 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     7861 gcccgcgtgg ttcctggcca ccgtcggagt ctcgcccgac caccagggca agggtctggg
     7921 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     7981 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     8041 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     8101 aacgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     8161 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     8221 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     8281 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     8341 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     8401 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     8461 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     8521 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     8581 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     8641 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     8701 cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     8761 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     8821 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     8881 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     8941 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     9001 atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     9061 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     9121 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     9181 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     9241 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     9301 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     9361 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     9421 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     9481 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     9541 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     9601 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     9661 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     9721 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     9781 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     9841 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     9901 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     9961 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
    10021 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
    10081 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
    10141 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
    10201 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
    10261 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
    10321 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
    10381 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
    10441 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
    10501 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
    10561 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
    10621 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
    10681 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
    10741 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    10801 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
    10861 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
    10921 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
    10981 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
    11041 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
    11101 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    11161 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    11221 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    11281 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    11341 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    11401 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    11461 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    11521 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    11581 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    11641 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    11701 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    11761 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    11821 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    11881 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    11941 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    12001 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    12061 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    12121 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    12181 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    12241 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    12301 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    12361 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    12421 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    12481 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    12541 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    12601 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    12661 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    12721 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    12781 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    12841 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    12901 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    12961 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    13021 ctgac
//
