LOCUS       Exported               13262 bp ds-DNA     circular SYN 15-JAN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 15, 2020 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13262
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             828..1016
                     /label=5' LTR
                     /note="5' LTR (R and U5 portions; U3 was replaced by the 
                     CMV promoter)"
     primer_bind     complement(986..1016)
                     /label=Primer 4
     misc_feature    1067..1204
                     /label=Psi
                     /note="Psi"
     misc_feature    1124..1127
                     /label=SD; splice donor
                     /note="SD; splice donor"
     misc_feature    1171..1535
                     /label=gag
                     /note="gag"
     primer_bind     complement(1356..1379)
                     /label=Primer 6
     misc_feature    1681..1922
                     /label=RRE
                     /note="RRE"
     primer_bind     complement(1955..1977)
                     /label=Primer 5
     misc_feature    2440..2557
                     /label=CPPT; central polypurine tract
                     /note="CPPT; central polypurine tract"
     promoter        2608..2857
                     /label=Human U6
     primer_bind     2608..2632
                     /label=Primer 7
     primer_bind     2608..2629
                     /label=Primer 9
     primer_bind     2782..2801
                     /label=Primer 12
     misc_feature    2873..2965
                     /label=sgRNA F+E
     misc_feature    2937..3008
                     /label=Untitled
     primer_bind     2937..2956
                     /label=Primer 10
     misc_feature    2943..3002
                     /label=barcode oligo
                     /note="barcode oligo"
     primer_bind     complement(2946..2958)
                     /label=Primer 10
     promoter        2984..3239
                     /label=EFS-NS
                     /note="EFS-NS"
     primer_bind     2984..3009
                     /label=Primer 1
     primer_bind     complement(2990..3009)
                     /label=Primer 11
     primer_bind     complement(3195..3217)
                     /label=Primer 8
                     /note="LentiCRISPR V2 (LCV2) PCR1 R"
     primer_bind     complement(3219..3239)
                     /label=Primer 2
     misc_feature    3266..3478
                     /label=KRAB
     misc_feature    3500..7603
                     /label=xCas9
                     /note="xCas9"
     misc_feature    3527..3529
                     /label=D10A
                     /note="D10A"
     misc_feature    4469..4471
                     /label=R324L
                     /note="R324L"
     misc_feature    4724..4726
                     /label=S409I
                     /note="S409I"
     misc_feature    4937..4939
                     /label=E480K
                     /note="E480K"
     misc_feature    5126..5128
                     /label=E543D
                     /note="E543D"
     misc_feature    5579..5581
                     /label=M694I
                     /note="M694I"
     primer_bind     5999..6028
                     /label=Primer 14
     primer_bind     complement(6007..6036)
                     /label=Primer 13
     misc_feature    6017..6019
                     /label=H840A
                     /note="H840A"
     misc_feature    7154..7156
                     /label=E1219V
                     /note="E1219V"
     misc_feature    7604..7651
                     /label=NLS
                     /note="NLS"
     misc_feature    7652..7675
                     /label=FLAG
                     /note="FLAG"
     misc_feature    7676..7741
                     /label=P2A
                     /note="P2A"
     CDS             7742..8338
                     /label=Puro
                     /note="Puro"
     primer_bind     7742..7763
                     /label=Primer 3
     misc_binding    8354..8942
                     /label=WPRE
                     /note="WPRE"
     LTR             9013..9248
                     /label=3' LTR SIN
     primer_bind     complement(9218..9248)
                     /label=Primer 4
     rep_origin      11697..11922
                     /direction=RIGHT
                     /label=ColE1
                     /note="ColE1"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg
     2641 catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag
     2701 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     2761 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     2821 ttggctttat atatcttgtg gaaaggacga aacaccggag acggacgtct ctgtttaaga
     2881 gctatgctgg aaacagcata gcaagtttaa ataaggctag tccgttatca acttgaaaaa
     2941 gtggcaccga gtcggtgctt tttttgaatt catgtaggct agctaggtct tgaaaggagt
     3001 gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     3061 gttgggggga ggggtcggca attgatccgg tgcctagaga aggtggcgcg gggtaaactg
     3121 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     3181 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagga
     3241 ccggttctag agcgctgcca ccatggatgc taagtcacta actgcctggt cccggacact
     3301 ggtgaccttc aaggatgtat ttgtggactt caccagggag gagtggaagc tgctggacac
     3361 tgctcagcag atcgtgtaca gaaatgtgat gctggagaac tataagaacc tggtttcctt
     3421 gggttatcag cttactaagc cagatgtgat cctccggttg gagaagggag aagagcccgg
     3481 cggttccggc ggagggtcga tggacaagaa gtacagcatc ggcctggcca tcggcaccaa
     3541 ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt
     3601 gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc tgctgttcga
     3661 cagcggcgaa acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag
     3721 acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga
     3781 cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga
     3841 gcggcacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac
     3901 catctaccac ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat
     3961 ctatctggcc ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct
     4021 gaaccccgac aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca
     4081 gctgttcgag gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc
     4141 cagactgagc aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa
     4201 gaatggcctg ttcggaaacc tgattgccct gagcctgggc ctgaccccca acttcaagag
     4261 caacttcgac ctggccgagg ataccaaact gcagctgagc aaggacacct acgacgacga
     4321 cctggacaac ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa
     4381 gaacctgtcc gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa
     4441 ggcccccctg agcgcctcta tgatcaagct gtacgacgag caccaccagg acctgaccct
     4501 gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca
     4561 gagcaagaac ggctacgccg gctacattga cggcggagcc agccaggaag agttctacaa
     4621 gttcatcaag cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa
     4681 cagagaggac ctgctgcgga agcagcggac cttcgacaac ggcatcatcc cccaccagat
     4741 ccacctggga gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa
     4801 ggacaaccgg gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc
     4861 tctggccagg ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac
     4921 cccctggaac ttcgagaagg tggtggacaa gggcgcttcc gcccagagct tcatcgagcg
     4981 gatgaccaac ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct
     5041 gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga ccgagggaat
     5101 gagaaagccc gccttcctga gcggcgacca gaaaaaggcc atcgtggacc tgctgttcaa
     5161 gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg
     5221 cttcgactcc gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata
     5281 ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga
     5341 cattctggaa gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga
     5401 acggctgaaa acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg
     5461 gagatacacc ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca
     5521 gtccggcaag acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat
     5581 ccagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc
     5641 cggccagggc gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa
     5701 gaagggcatc ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca
     5761 caagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca
     5821 gaagaacagc cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca
     5881 gatcctgaaa gaacaccccg tggaaaacac ccagctgcag aacgagaagc tgtacctgta
     5941 ctacctgcag aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc
     6001 cgactacgat gtggacgcta tcgtgcctca gagctttctg aaggacgact ccatcgacaa
     6061 caaggtgctg accagaagcg acaagaaccg gggcaagagc gacaacgtgc cctccgaaga
     6121 ggtcgtgaag aagatgaaga actactggcg gcagctgctg aacgccaagc tgattaccca
     6181 gagaaagttc gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc
     6241 cggcttcatc aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat
     6301 cctggactcc cggatgaaca ctaagtacga cgagaatgac aagctgatcc gggaagtgaa
     6361 agtgatcacc ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa
     6421 agtgcgcgag atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg
     6481 aaccgccctg atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa
     6541 ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc
     6601 caagtacttc ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa
     6661 cggcgagatc cggaagcggc ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg
     6721 ggataagggc cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat
     6781 cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa gagtctatcc tgcccaagag
     6841 gaacagcgat aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt
     6901 cgacagcccc accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc
     6961 caagaaactg aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt
     7021 cgagaagaat cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct
     7081 gatcatcaag ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct
     7141 ggcctctgcc ggcgtgctgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa
     7201 cttcctgtac ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca
     7261 gaaacagctg tttgtggaac agcacaagca ctacctggac gagatcatcg agcagatcag
     7321 cgagttctcc aagagagtga tcctggccga cgctaatctg gacaaagtgc tgtccgccta
     7381 caacaagcac cgggataagc ccatcagaga gcaggccgag aatatcatcc acctgtttac
     7441 cctgaccaat ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa
     7501 gaggtacacc agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg
     7561 cctgtacgag acacggatcg acctgtctca gctgggaggc gacaagcgac ctgccgccac
     7621 aaagaaggct ggacaggcta agaagaagaa agattacaaa gacgatgacg ataagggatc
     7681 cggcgcaaca aacttctctc tgctgaaaca agccggagat gtcgaagaga atcctggacc
     7741 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     7801 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     7861 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     7921 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     7981 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     8041 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     8101 cgcgtggttc ctggccaccg tcggagtctc gcccgaccac cagggcaagg gtctgggcag
     8161 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     8221 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     8281 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaac
     8341 gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     8401 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     8461 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     8521 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     8581 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     8641 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     8701 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     8761 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     8821 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     8881 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     8941 gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     9001 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     9061 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     9121 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     9181 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     9241 tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     9301 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     9361 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     9421 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     9481 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     9541 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     9601 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     9661 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     9721 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     9781 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     9841 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     9901 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     9961 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
    10021 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
    10081 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
    10141 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
    10201 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
    10261 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
    10321 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat
    10381 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca
    10441 agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct
    10501 ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc
    10561 gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc
    10621 tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt
    10681 ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg
    10741 ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc
    10801 aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
    10861 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
    10921 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    10981 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    11041 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
    11101 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
    11161 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
    11221 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
    11281 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
    11341 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    11401 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    11461 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    11521 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    11581 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    11641 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    11701 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    11761 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    11821 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    11881 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    11941 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    12001 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    12061 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    12121 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    12181 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    12241 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    12301 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    12361 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    12421 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    12481 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    12541 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    12601 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    12661 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    12721 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    12781 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    12841 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    12901 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    12961 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    13021 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    13081 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    13141 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    13201 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    13261 ac
//
