LOCUS       Exported                4643 bp ds-DNA     circular SYN 07-JUL-2020
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Brandon Cisneros
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 7, 2020 from SnapGene Viewer 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    6..230
                     /label=5'-ITR (Sleeping Beauty)
                     /note="5'-ITR"
                     /note="Sleeping Beauty inverted tandem repeat"
     misc_feature    236..277
                     /label=5'-proxITR (Sleeping Beauty)
                     /note="5'-proxITR"
                     /note="modified region proximal to 5'-ITR - contains CTCF 
                     binding sites"
     misc_feature    308..515
                     /label=Stat3 Operator Promoter
     misc_feature    308..366
                     /label=op3
     promoter        388..426
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate earlypromoter"
     primer_bind     423..447
                     /label=LNCX Primer
     misc_feature    527..539
                     /label=5'-SfiI
                     /note="5'-SfiI"
                     /note="GGCCTCTGAGGCC"
     regulatory      537..546
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong 
                     initiationof translation (Kozak, 1987)"
     misc_feature    540..735
                     /label=filler DNA
                     /note="filler DNA"
     misc_feature    736..748
                     /label=3'-SfiI
                     /note="3'-SfiI"
                     /note="GGCCTGTCAGGCC"
     polyA_signal    766..887
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(777..796)
                     /label=EBV Rev (SV40 pA Term. Rev)
     primer_bind     831..850
                     /label=SV40pA-R
     promoter        1012..1623
                     /label=RPBSA
     regulatory      1643..1648
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong 
                     initiationof translation (Kozak, 1987)"
     CDS             1649..2023
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVLVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     complement(2078..2095)
                     /label=bGH pA Primer
     polyA_signal    2084..2308
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    2351..2392
                     /label=3'-proxITR (Sleeping Beauty)
                     /note="3'-proxITR"
                     /note="modified region proximal to 3'-ITR - contains CTCF 
                     binding sites"
     misc_feature    2393..2617
                     /label=3'-ITR (Sleeping Beauty)
                     /note="3'-ITR"
                     /note="Sleeping Beauty inverted tandem repeat"
     rep_origin      complement(2805..3393)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3564..4424)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4425..4529)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctatacagtt gaagtcggaa gtttacatac acttaagttg gagtcattaa aactcgtttt
       61 tcaactactc cacaaatttc ttgttaacaa acaatagttt tggcaagtca gttaggacat
      121 ctactttgtg catgacacaa gtcatttttc caacaattgt ttacagacag attatttcac
      181 ttataattca ctgtatcaca attccagtgg gtcagaagtt tacatacact aagttcgact
      241 cctctgcaga atgcggcgat gtttcggtaa ggggtccgct atctagtgag ctcttacgcg
      301 tgctagccgt cgacatttcc cgtaaatcgt cgagtcgaca tttcccgtaa atcgtcgacc
      361 tgcaggtcga gctcggtacc cgggtcgagt aggcgtgtac ggtgggaggc ctatataagc
      421 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
      481 catagaagac accgggaccg atccagcctc cgcgggaatt caagctggcc tctgaggcca
      541 ccatggctcg agtttcgaac tcaattatac tgaattgttt catcataggg ttcaacattg
      601 tgataaatca ggacttcaat gtctagtgtt cagtaaatac cttgtagggc tctccaaaaa
      661 ggggaatctt ttcaggaaat gcctctttct cttggtgggc ttcctggttg cgtctttcct
      721 tctcactaat tcgaaggcct gtcaggccgc ttcgagcaga catgataaga tacattgatg
      781 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
      841 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
      901 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
      961 acctctacaa atgtggtaaa atcgataagg atccgtcgac cggtacctag gcgagaccct
     1021 gtctcacaaa ataaagtaag cccggactga gtgcggaaag gcgggcctgg cgggtctggt
     1081 ctccccatgc gggccaccag aggccctgca gccttcagtc gcttgaaggg gtaatggcgc
     1141 ttccactcac aaacatggcg gacagagcgt gtgaacgaga tgaacagccc ctcaaaaata
     1201 tggccgccga ggctggacgg ccgtgcccca gcagcaccgc ctccgcgccc cacgtgatct
     1261 ctcgccgggc acagcgctga ccgcggaggt ccaaccggaa gaatgtccgg attggacatt
     1321 cggaagaggg cccgccttcc ctggggaatc tctgcgcacg cgcagaacgc ttcgaccaat
     1381 gaaaacacag gaagccgtcc gcgcaaccgc gttgcgtcac ttctgccgcc cctgtttcaa
     1441 ggtatatagc cgtagacgga acttcgcctt tctctcggcc ttagcgccat ttttttgggt
     1501 gagtgttttt tggttcctgc gttgggattc cgtgtacaat ccatagacat ctgacctcgg
     1561 cacttagcat catcacagca aactaactgt agcctttctc tctttccctg tagaaacctc
     1621 tgcacctgag gccaccaaac tagccaccat ggccaagttg accagtgccg ttccggtgct
     1681 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg
     1741 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag
     1801 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgttgg tgcgcggcct
     1861 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg
     1921 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc
     1981 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgattcgaag gcctgtcgtg
     2041 aagcttgggg atcaattctc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc
     2101 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     2161 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     2221 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     2281 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctgggcctac
     2341 tagctactcg ggacccctta ccgaaacatc gccgcattct gcagaggagt cgagtgtatg
     2401 taaacttctg acccactggg aatgtgatga aagaaataaa agctgaaatg aatcattctc
     2461 tctactatta ttctgatatt tcacattctt aaaataaagt ggtgatccta actgacctaa
     2521 gacagggaat ttttactagg attaaatgtc aggaattgtg aaaaagtgag tttaaatgta
     2581 tttggctaag gtgtatgtaa acttccgact tcaactgtat agggatccgc ttcctcgctc
     2641 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     2701 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     2761 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     2821 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2881 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2941 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     3001 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3061 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3121 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3181 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3241 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3301 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3361 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3421 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3481 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3541 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     3601 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     3661 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     3721 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     3781 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     3841 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     3901 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     3961 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     4021 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     4081 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     4141 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     4201 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     4261 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     4321 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     4381 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     4441 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     4501 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgatgcg
     4561 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt
     4621 aatattttgt taaaattgga tcc
//
