LOCUS       Exported                6123 bp ds-DNA     circular SYN 16-JAN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jan 17, 2020 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
COMMENT     Y531F_Fwd: GAGCCCCAGTtCCAACCTGGT
            Y531F_rev: TGTCGCGGTAAAGTAGTCTTC
            Ta is 66deg
            
            osm-10p_src2_F: ggtggcggccgctctagaactagtCTGCAGCTTGACACCGACTGGCACAC
            osm-10p_Fyn_R: CTTACATTGCACACAGCCCATGACATCTTGCAAGATGAAATTTCCAAAAAAG
            
            Fyn_osm10p_F: GAAATTTCATCTTGCAAGATGTCATGGGCTGTGTGCAATGTAAGGATAAAGAAG
            Fyn_unc54UTR_R: gttgaagagtaattggacTTACAGGTTTTCACCAGGTTGGTACTGGGGCTC
            
            unc54UTR_Fyn_F: 
            GTACCAACCTGGTGAAAACCTGTAAgtccaattactcttcaacatccctacatg
            unc54UTR_src2_R: 
            ctgggtaccgggccccccctcgagaaacagttatgtttggtatattgggaatg
            
            Empty:
            F: GTCCAATTACTCTTCAACATC
            R: CTTGCAAGATGAAATTTCC
            Ta = 57
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..307
                     /locus_tag="F1 Ori"
                     /label=F1 Ori
     misc_feature    326..394
                     /locus_tag="lacz a"
                     /label=lacz a
     misc_feature    465..482
                     /locus_tag="M13F"
                     /label=M13F
     misc_feature    531..580
                     /locus_tag="osm-10_F"
                     /label=osm-10_F
     misc_feature    543..548
                     /locus_tag="XbaI"
                     /label=XbaI
     misc_feature    555..1454
                     /locus_tag="osm-10p from sequencing"
                     /label=osm-10p from sequencing
     misc_feature    1337..1357
                     /locus_tag="osm-10p F"
                     /label=osm-10p F
     misc_feature    1424..1475
                     /locus_tag="osm-10p_Fyn_R"
                     /label=osm-10p_Fyn_R
     misc_feature    1432..1485
                     /locus_tag="Fyn_osm10p_F"
                     /label=Fyn_osm10p_F
     misc_feature    1455..3065
                     /locus_tag="Fyn"
                     /label=Fyn
     misc_feature    3066..3767
                     /locus_tag="unc-54 utr in melissa's"
                     /label=unc-54 utr in melissa's
     misc_feature    3739..3791
                     /locus_tag="unc-54UTR_R"
                     /label=unc-54UTR_R
     misc_feature    3836..3856
                     /locus_tag="M13R"
                     /label=M13R
     misc_feature    3862..3884
                     /locus_tag="LacO"
                     /label=LacO
     misc_feature    4224..4906
                     /locus_tag="ColE1 Ori"
                     /label=ColE1 Ori
     misc_feature    5004..5663
                     /locus_tag="AmpR"
                     /label=AmpR
ORIGIN
        1 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
       61 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
      121 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
      181 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
      241 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
      301 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
      361 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      421 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      481 gtgagcgcgc gtaatacgac tcactatagg gcgaattgga gctccaccgc ggtggcggcc
      541 gctctagaac tagtctgcag cttgacaccg actggcacac acaaaatatt aaactttcag
      601 ggtctaatcc atcacgaaat gaacgaccac aacctcaatg caaaatgtca tttatgccag
      661 aaatctttcg agaataagaa gacattccgt gaacactgtg aattggatca cacaccggaa
      721 gtggtatgac agtattcatt aacagattca atttgaaata ttaattttag gttagatgtg
      781 tattctgcaa aagctcattc gatcagccac tggaaatgaa cgatgaacaa tgggcacact
      841 ttttcgctca tatttatggg gttagtaatt caccattcga atctagaagt cgattctcct
      901 gactgacatt tttaaaacca ttttttccag gaaatcctct actcccgcct tgccgactac
      961 gaggatcgtg ctttgaacac tgcatgaatt atatatttga ttcgatggtt agcttaataa
     1021 agaatttttc tgttgtactt caaccttttt ctgaaatagg aaataatcac aagttgtctg
     1081 aaaaatgtga tcagctatcc agatcaaaca gtgaaaatgt tgtctgcaat gattcatagc
     1141 tgaacatctg aactcttaaa ttttcagcga atcatgattt gcattgtgat atattattct
     1201 tatggttgca aaagaaaaaa gattacgaaa atatcttgtg acagagtaag aaaccattga
     1261 aatttaatta atcaccgtgg agacaggtgt gcactctgct cttattatat tatcaaaacg
     1321 agaaacgcgt gagcaagaag tgggcgtgac ttggtagaaa ggtacttaat cgagatgttg
     1381 agccaacttt cgagattatt ctaaaagttn cnatatcaat aaactttttt ggaaatttca
     1441 tcttgcaaga tgtcatgggc tgtgtgcaat gtaaggataa agaagcaaca aaactgacgg
     1501 aggagaggga cggcagcctg aaccagagct ctgggtaccg ctatggcaca gaccccaccc
     1561 ctcagcacta ccccagcttc ggtgtgacct ccatccccaa ctacaacaac ttccacgcag
     1621 ccgggggcca aggactcacc gtctttggag gtgtgaactc ttcgtctcat acggggacct
     1681 tgcgtacgag aggaggaaca ggagtgacac tctttgtggc cctttatgac tatgaagcac
     1741 ggacagaaga tgacctgagt tttcacaaag gagaaaaatt tcaaatattg aacagctcgg
     1801 aaggagattg gtgggaagcc cgctccttga caactggaga gacaggttac attcccagca
     1861 attatgtggc tccagttgac tctatccagg cagaagagtg gtactttgga aaacttggcc
     1921 gaaaagatgc tgagcgacag ctattgtcct ttggaaaccc aagaggtacc tttcttatcc
     1981 gcgagagtga aaccaccaaa ggtgcctatt cactttctat ccgtgattgg gatgatatga
     2041 aaggagacca tgtcaaacat tataaaattc gcaaacttga caatggtgga tactacatta
     2101 ccacccgggc ccagtttgaa acacttcagc agcttgtaca acattactca gagagagctg
     2161 caggtctctg ctgccgccta gtagttccct gtcacaaagg gatgccaagg cttaccgatc
     2221 tgtctgtcaa aaccaaagat gtctgggaaa tccctcgaga atccctgcag ttgatcaaga
     2281 gactgggaaa tgggcagttt ggggaagtat ggatgggtac ctggaatgga aacacaaaag
     2341 tagccataaa gactcttaaa ccaggcacaa tgtcccccga atcattcctt gaggaagcgc
     2401 agatcatgaa gaagctgaag cacgacaagc tggtccagct ctatgcagtg gtgtctgagg
     2461 agcccatcta catcgtcacc gagtatatga acaaaggaag tttactggat ttcttaaaag
     2521 atggagaagg aagagctctg aaattaccaa atcttgtgga catggcagca caggtggctg
     2581 caggaatggc ttacatcgag cgcatgaatt atatccatag agatctgcga tcagcaaaca
     2641 ttctagtggg gaatggactc atatgcaaga ttgctgactt cggattggcc cgattgatag
     2701 aagacaatga gtacacagca agacaaggtg caaagttccc catcaagtgg acggcccccg
     2761 aggcagccct gtacgggagg ttcacaatca agtctgacgt gtggtctttt ggaatcttac
     2821 tcacagagct ggtcaccaaa ggaagagtgc catacccagg catgaacaac cgggaggtgc
     2881 tggagcaggt ggagcgaggc tacaggatgc cctgcccgca ggactgcccc atctctctgc
     2941 atgagctcat gatccactgc tggaaaaagg accctgaaga acgccccact tttgagtact
     3001 tgcagagctt cctggaagac tactttaccg cgacagagcc ccagtaccaa cctggtgaaa
     3061 acctgtaagt ccaattactc ttcaacatcc ctacatgctc tttctccctg tgctcccacc
     3121 ccctattttt gttattatca aaaaacttct cttaatttct ttgtttttta gcttctttta
     3181 agtcacctct aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg taataaaaag
     3241 tcgaaaaaaa ttgtgctccc tccccccatt aataataatt ctatcccaaa atctacacaa
     3301 tgttctgtgt acacttctta tgttttttac ttctgataaa tttttttgaa acatcataga
     3361 aaaaaccgca cacaaaatac cttatcatat gttacgtttc agtttatgac cgcaattttt
     3421 atttcttcgc acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt
     3481 ggagtatttt tggaattttt caatcaagtg aaagtttatg aaattaattt tcctgctttt
     3541 gctttttggg gtttccccta ttgtttgtca agatttcgag gacggcgttt ttcttgctaa
     3601 aatcacaagt attgatgagc acgatgcaag aaagatcgga agaaggtttg ggtttgaggc
     3661 tcagtggaag gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta tggggttttt
     3721 gccttaaatg acagaataca ttcccaatat accaaacata actgtttctc gagggggggc
     3781 ccggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
     3841 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     3901 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     3961 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     4021 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     4081 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     4141 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     4201 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4261 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4321 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4381 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     4441 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4501 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4561 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4621 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4681 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     4741 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4801 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4861 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     4921 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     4981 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     5041 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     5101 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     5161 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     5221 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     5281 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     5341 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     5401 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     5461 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     5521 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     5581 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     5641 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     5701 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     5761 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     5821 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     5881 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     5941 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
     6001 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     6061 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     6121 gtg
//
