LOCUS       Exported                6351 bp ds-DNA     circular SYN 16-JAN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jan 17, 2020 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
COMMENT     Fyn_F: ccggtagaaaaaATGGGCTGTGTGCAATGTAAGG
            Fyn_R (rev comp): cggtggcggccgctctagaactagtaagcttcaatacaag
            
            mec4_Fyn_F: cgctctagaactagtaagcttcaatacaagctcaaatac
            mec4_Fyn_R (rev comp): gcttggagggtaccggtagaaaaaATGGGCTGTGTGCAATG
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..307
                     /locus_tag="F1 Ori"
                     /label=F1 Ori
     misc_feature    326..394
                     /locus_tag="lacz a"
                     /label=lacz a
     misc_feature    465..482
                     /locus_tag="M13F"
                     /label=M13F
     misc_feature    543..548
                     /locus_tag="XbaI"
                     /label=XbaI
     promoter        555..1574
                     /locus_tag="mec-4\Promoter"
                     /label=mec-4\Promoter
                     /note="/vntifkey=29"
     misc_feature    1683..3293
                     /locus_tag="Fyn"
                     /label=Fyn
     misc_feature    3294..3995
                     /locus_tag="unc-54 utr in melissa's"
                     /label=unc-54 utr in melissa's
     misc_feature    4064..4084
                     /locus_tag="M13R"
                     /label=M13R
     misc_feature    4090..4112
                     /locus_tag="LacO"
                     /label=LacO
     misc_feature    4452..5134
                     /locus_tag="ColE1 Ori"
                     /label=ColE1 Ori
     misc_feature    5232..5891
                     /locus_tag="AmpR"
                     /label=AmpR
ORIGIN
        1 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
       61 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
      121 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
      181 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
      241 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
      301 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
      361 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      421 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      481 gtgagcgcgc gtaatacgac tcactatagg gcgaattgga gctccaccgc ggtggcggcc
      541 gctctagaac tagtaagctt caatacaagc tcaaatacaa tactattttt tttccgtctg
      601 aacataaaat tagtttctaa ataaaaaatg tgaaaataaa tatttggttt taatcgttaa
      661 aatcttccaa attactaata aagttcagtc aactatatgc ttcgaaattc actgaatcta
      721 ttattgttat taatttttag acccttggag agctcccaac tctttcaatc cagaagactg
      781 atggatgcca ggtgtacttg tcaaaggtgg ctcaaggatg cgagattgtc acttccaaat
      841 ccagtgagat gaacatctcc gtgcaaacca atgatgatgg ggattatgta agtttttcgg
      901 attgtagaac ccaatctaaa tttccttttc agtcggagtt cccggttccg gagcagttca
      961 agacaacttt tgtcaatggc aagctagtca ctgtcgtctc ggacatttgc taaatcttct
     1021 cacgtcataa ccttattttt caacttcatg aactatcccc tcgaactttt tttttgtccc
     1081 tgtctacaat tctttataag caattgagac acactttgct ttattaaata ataccgattt
     1141 tgttttgtat atgcattgac tggtgctcaa ctatttttat tgaaaaaatt actttttact
     1201 gtctcgtcga ataaaagtga tcccttccaa aaaatagtta ttcatgtttg aaggtttgtg
     1261 atcgtagaac aattgcaccg aatgtagtgt aaaaaaaatt ccagaatgtt caaatacatt
     1321 tccaccctca ccagaacatc tcaaacacca ggcacatttc aacacacttt catggatctt
     1381 atcttgcctc aaaaccatca gctttcttct cattctacaa gtatgaaaca atctactatt
     1441 ctatacattt ataatgtaat ggaaccatgt gaagacatga gcaagatcat tgtcgtcagc
     1501 gactacccct ccttcatttt tcttagagta tcagtttgct aatgctattt tttttatcgc
     1561 tatcaagtta tagagggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg
     1621 atactaacat aacatagaac attttcagga ggacccttgc ttggagggta ccggtagaaa
     1681 aaatgggctg tgtgcaatgt aaggataaag aagcaacaaa actgacggag gagagggacg
     1741 gcagcctgaa ccagagctct gggtaccgct atggcacaga ccccacccct cagcactacc
     1801 ccagcttcgg tgtgacctcc atccccaact acaacaactt ccacgcagcc gggggccaag
     1861 gactcaccgt ctttggaggt gtgaactctt cgtctcatac ggggaccttg cgtacgagag
     1921 gaggaacagg agtgacactc tttgtggccc tttatgacta tgaagcacgg acagaagatg
     1981 acctgagttt tcacaaagga gaaaaatttc aaatattgaa cagctcggaa ggagattggt
     2041 gggaagcccg ctccttgaca actggagaga caggttacat tcccagcaat tatgtggctc
     2101 cagttgactc tatccaggca gaagagtggt actttggaaa acttggccga aaagatgctg
     2161 agcgacagct attgtccttt ggaaacccaa gaggtacctt tcttatccgc gagagtgaaa
     2221 ccaccaaagg tgcctattca ctttctatcc gtgattggga tgatatgaaa ggagaccatg
     2281 tcaaacatta taaaattcgc aaacttgaca atggtggata ctacattacc acccgggccc
     2341 agtttgaaac acttcagcag cttgtacaac attactcaga gagagctgca ggtctctgct
     2401 gccgcctagt agttccctgt cacaaaggga tgccaaggct taccgatctg tctgtcaaaa
     2461 ccaaagatgt ctgggaaatc cctcgagaat ccctgcagtt gatcaagaga ctgggaaatg
     2521 ggcagtttgg ggaagtatgg atgggtacct ggaatggaaa cacaaaagta gccataaaga
     2581 ctcttaaacc aggcacaatg tcccccgaat cattccttga ggaagcgcag atcatgaaga
     2641 agctgaagca cgacaagctg gtccagctct atgcagtggt gtctgaggag cccatctaca
     2701 tcgtcaccga gtatatgaac aaaggaagtt tactggattt cttaaaagat ggagaaggaa
     2761 gagctctgaa attaccaaat cttgtggaca tggcagcaca ggtggctgca ggaatggctt
     2821 acatcgagcg catgaattat atccatagag atctgcgatc agcaaacatt ctagtgggga
     2881 atggactcat atgcaagatt gctgacttcg gattggcccg attgatagaa gacaatgagt
     2941 acacagcaag acaaggtgca aagttcccca tcaagtggac ggcccccgag gcagccctgt
     3001 acgggaggtt cacaatcaag tctgacgtgt ggtcttttgg aatcttactc acagagctgg
     3061 tcaccaaagg aagagtgcca tacccaggca tgaacaaccg ggaggtgctg gagcaggtgg
     3121 agcgaggcta caggatgccc tgcccgcagg actgccccat ctctctgcat gagctcatga
     3181 tccactgctg gaaaaaggac cctgaagaac gccccacttt tgagtacttg cagagcttcc
     3241 tggaagacta ctttaccgcg acagagcccc agtaccaacc tggtgaaaac ctgtaagtcc
     3301 aattactctt caacatccct acatgctctt tctccctgtg ctcccacccc ctatttttgt
     3361 tattatcaaa aaacttctct taatttcttt gttttttagc ttcttttaag tcacctctaa
     3421 caatgaaatt gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt
     3481 gtgctccctc cccccattaa taataattct atcccaaaat ctacacaatg ttctgtgtac
     3541 acttcttatg ttttttactt ctgataaatt tttttgaaac atcatagaaa aaaccgcaca
     3601 caaaatacct tatcatatgt tacgtttcag tttatgaccg caatttttat ttcttcgcac
     3661 gtctgggcct ctcatgacgt caaatcatgc tcatcgtgaa aaagttttgg agtatttttg
     3721 gaatttttca atcaagtgaa agtttatgaa attaattttc ctgcttttgc tttttggggt
     3781 ttcccctatt gtttgtcaag atttcgagga cggcgttttt cttgctaaaa tcacaagtat
     3841 tgatgagcac gatgcaagaa agatcggaag aaggtttggg tttgaggctc agtggaaggt
     3901 gagtagaagt tgataatttg aaagtggagt agtgtctatg gggtttttgc cttaaatgac
     3961 agaatacatt cccaatatac caaacataac tgtttctcga gggggggccc ggtacccagc
     4021 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
     4081 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     4141 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     4201 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     4261 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     4321 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     4381 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     4441 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     4501 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     4561 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     4621 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     4681 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4741 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     4801 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     4861 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     4921 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     4981 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5041 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     5101 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     5161 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     5221 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     5281 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     5341 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     5401 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     5461 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     5521 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     5581 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     5641 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     5701 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     5761 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     5821 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     5881 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     5941 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     6001 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     6061 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     6121 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     6181 ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt
     6241 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     6301 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt g
//
