LOCUS       Exported               13019 bp ds-DNA     circular SYN 18-NOV-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13019)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 20, 2020 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13019
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..23
                     /label=oMT462
     misc_feature    4..1948
                     /label=1 to 4915 of pNBU2_ermGb
                     /note="1 to 4915 of pNBU2_ermGb"
     misc_feature    4..36
                     /label=MCS
                     /note="MCS"
     gene            complement(273..1553)
                     /label=NBU2 integrase
                     /note="NBU2 integrase"
     primer_bind     765..784
                     /label=oMT1411
     misc_feature    1209
                     /label=mutated to A in actual construct
     misc_feature    1375
                     /label=mutated to T in actual construct
     misc_feature    1443
                     /label=mutated to A in actual construct
     misc_feature    1679
                     /label=mutated to T in actual construct
     misc_feature    1713..1725
                     /label=attN2
                     /note="attN2"
     misc_feature    1794
                     /label=mutated to G in actual construct
     misc_feature    1905
                     /label=mutated to C in actual construct
     primer_bind     complement(1941..1957)
                     /label=oMT884
     misc_feature    1949..5857
                     /note="891 to 4799 of pKNOCK_ApTet (Mlu fragment 
                     orientation may be switched)"
     misc_feature    1949..1954
                     /label=MluI
                     /note="MluI"
     primer_bind     complement(1968..1987)
                     /label=oMT1416
     gene            2510..4435
                     /label=tetQ
                     /note="tetQ"
     primer_bind     complement(2749..2768)
                     /label=oMT1415
     primer_bind     complement(3521..3540)
                     /label=oMT1414
     primer_bind     complement(4317..4337)
                     /label=oMT1413
     misc_feature    4577..4581
                     /label=deleted in actual construct
     misc_feature    4833..4836
                     /label=deleted in actual construct
     gene            4968..5829
                     /label=bla
                     /note="bla"
     primer_bind     complement(5066..5085)
                     /label=oMT1412
     primer_bind     complement(5833..5857)
                     /label=oMT885
     misc_feature    5852..5857
                     /label=MluI
                     /note="MluI"
     misc_feature    5858..6652
                     /label=1 to 4915 of pNBU2_ermGb
                     /note="1 to 4915 of pNBU2_ermGb"
     misc_feature    5867..6652
                     /label=RP4 oriT/R6K oriV
                     /note="RP4 oriT/R6K oriV"
     primer_bind     6558..6577
                     /label=oMT25
     primer_bind     6635..6817
                     /label=oMT1305
     primer_bind     complement(6635..6652)
                     /label=oMT1013
     terminator      6653..6709
                     /label=Terminator L3S2P55 terminator
                     /label=Terminator L3S2P55
     misc_feature    6671
                     /label=deleted in actual construct
     primer_bind     complement(6688..6732)
                     /label=oMT1286
     primer_bind     6710..6817
                     /label=oMT1177
     misc_feature    6710..6753
                     /label=P1 promoter
     misc_feature    6710..6731
                     /label=Feature 56
     misc_feature    6717..6720
                     /label=-33
     misc_feature    6742..6749
                     /label=-7
     misc_feature    6754..6785
                     /label=RBS
     misc_feature    6754
                     /label=+1
     misc_feature    6786..10892
                     /label=pNBU2-Lac-dCas9-NL3 PCR Product - pNBU2-Lac-dCa
                     /label=pNBU2-Lac-dCas9-NL3 PCR Product - 
                     pNBU2-Lac-dCas9-NL3 PCR Product 3
                     /note="Geneious type: Inserted sequence"
     CDS             6786..10892
                     /codon_start=1
                     /label=cas9
                     /note="/Transferred_From=MBP-Cas9 Strep pyogenes PCR 
                     Product
                     /Transferred_Similarity=100.00%
                     /created_by=User"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGEFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     modified_base   6814
                     /label=D10A
                     /note="/created_by=User"
     primer_bind     6997..7017
                     /label=oMT94
     primer_bind     complement(7237..7257)
                     /label=oMT108
     primer_bind     7620..7639
                     /label=oMT95
     primer_bind     complement(8029..8046)
                     /label=oMT107
     primer_bind     8380..8399
                     /label=oMT96
     primer_bind     complement(8924..8943)
                     /label=oMT106
     modified_base   9303..9304
                     /label=H840A
                     /note="/created_by=User"
     misc_feature    9303..9304
                     /label=CA
                     /note="Geneious type: Editing History Replacement
                     /Original_Bases=CA"
     misc_feature    9305
                     /note="/created_by=User"
     misc_feature    9503
                     /label=mutated to a to t (gly to gly)
     primer_bind     complement(9758..9777)
                     /label=oMT105
     primer_bind     9958..9977
                     /label=oMT98
     misc_feature    10095..10097
                     /label=mutated gga to gaa (gly to gu)
     primer_bind     complement(10554..10574)
                     /label=oMT104
     primer_bind     10727..10747
                     /label=oMT99
     primer_bind     complement(10869..10892)
                     /label=oMT921
     primer_bind     10875..11053
                     /label=oMT1178
     misc_feature    10875..10892
                     /label=Feature 53
     promoter        10893..11029
                     /label=BF2884_promoter_region
     misc_feature    10965..10968
                     /label=-33?
     misc_feature    10989..10996
                     /label=-7?
     misc_feature    10997..11029
                     /label=RBS?
     CDS             11030..12121
                     /codon_start=1
                     /label=lacIq
                     /note="Transferred_From=LacIq Casette PCR Product
                     Transferred_Similarity=100.00%
                     created_by=User"
                     /translation="VVNVKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAM
                     AELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVER
                     SGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGD
                     WSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTED
                     SSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTA
                     SPRALADSLMQLARQVSRLESGQ"
     primer_bind     complement(11491..11510)
                     /label=oMT102
     primer_bind     11833..11852
                     /label=oMT101
     primer_bind     12104..12153
                     /label=oMT1311
     misc_feature    12104..12121
                     /label=Feature 57
     primer_bind     complement(12104..12121)
                     /label=oMT1179
     misc_feature    12122..12261
                     /label=P2
     misc_feature    12122..12124
                     /label=Feature 66
     misc_feature    12129..12307
                     /label=gblock
     misc_feature    12129..12132
                     /label=AGGC
     misc_feature    12262..12303
                     /label=A21
     CDS             12304..12927
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     misc_feature    12304..12307
                     /label=ATGT
     primer_bind     complement(12377..12396)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(12898..12945)
                     /label=oMT1182
     primer_bind     12928..12955
                     /label=oMT776
     terminator      join(12971..13019,1..3)
                     /label=Terminator
     primer_bind     complement(join(12994..13019,1..20))
                     /label=oMT461
     primer_bind     complement(join(13011..13019,1..10))
                     /label=oMT983
     primer_bind     complement(join(13012..13019,1..10))
                     /label=oMT986
     primer_bind     complement(join(13012..13019,1..10))
                     /label=oMT1043
ORIGIN
        1 ctacggccgc gatcccccgg gctgcaggaa ttcgatatca agcttatcga taccgtcgac
       61 taattgccta tcttccagtg atggaacagc atttgtgcat tggctgcaac aatcagcctt
      121 acttgtgcct gttctatttc cgaaccgacc gcttgtatga atgcatcaaa attcgttttc
      181 tctacgttgg attccttgtt gctcatattg tgatgataat ttctacaaat atagtcattg
      241 gtaactatct atgaaactgt ttgatacttt tatagttgat taaacttgtt catggcattt
      301 gccttaatat catccgctat gtcaatgtag ggtttcatag ctttgtagtc gctgtgtccc
      361 gtccatttca tgaccacctg tgccgggatt ccgagagcca gcgcattgca gatgaatgtc
      421 ctttttcctg catgggtact gagcaaagcg tatttgggtg tgacttcatc aatacgttca
      481 tttcccttgt agtaggtttc ccgtacaggc tcgttgattt ctgccagttc gcccagctct
      541 ttcaggtaat cgttcatctt ctggttgctg atgacgggca gagccatgta attctcgaaa
      601 tggatgtcct tgtatttgtc cagtatggct ttgctgtatt tgttcagttc aatcgtcagg
      661 ctgtcggcag tcttgactgt ggttatttcg atgtggtcgg acttcacatc gcttcttttc
      721 agattgcgaa catctgaata ccgcaaactc gtaaagcagc agaacaggaa aacatcacgc
      781 acacgttcca ggtattgctt atccttgggt atctggtagt ctttcagctt gttcagttca
      841 tcccaagtca ggaagattac ttttttcgag gtggttttca gtttcggttt gaacgtatcg
      901 tatgcaatgt tctgatgatg tcctttcttg aagctccagc gcaggaacca tttgaggaat
      961 cccatttgct tgccgatggt gctgtttctc atatccttgg tgtcacgcag gaagttgacg
     1021 tattcgttca atccaaactc gttgaaatag ttgaacgttg catcctcctt gaactctttg
     1081 aggtggttcc tcactgctgc aaatttttca taggtggatg ccgtccagtt attctggtta
     1141 ccgcactctt ttacaaactc atcgaacacc tcccaaaagc tgacaggggc ttcttccggc
     1201 tgttcttcgc tggtgtcttt cattctcatg ttgaaagctt ccttcaactg ttgggtcgtt
     1261 ggcatgacct cctgcacctc aaattccttg aaaatattct ggatttcggc atagtatttc
     1321 agcaagtccg tattgatttc ggctgcactt tgctttagct tgttggtaca tccgctcttt
     1381 acccgctgct tatctgcatc ccatttggct acgtcaatcc ggtagcccgt tgtaaactcg
     1441 atgcgttggc tggcaaagat gacacgcata cggatgggta cgttctctac gattggcaca
     1501 ccgttctttt tccggctctc caatgcaaaa atgatgttgc gcttgatatt cataattggg
     1561 tgcgtttgaa attctacacc caaatataca cccaattatt gagatagcaa aagacattta
     1621 gaaacattta cttttactct atattgtaat ttacacttga ttatcagtcg tttgcagtct
     1681 tatgatattc tgtgaaagta taagttcgag agcctgtctc tccgcaaaaa acgctgaaaa
     1741 tcagcagatt gcaaaacaaa caccctgttt tacacccaag aatgtaaagt cggctgtttt
     1801 tgttttattt aagataatac aaccactaca taataaaaga gtagcgatat taaaagaatc
     1861 cgatgagaaa agactaatat ttatctatcc attcagtttg atttttcagg actttacatc
     1921 gtcctgaaag tatttgttgg taccgaggac gcgttatctc cttaacgtac gttttcgttc
     1981 cattggccct caaaccccgt tatatacatt catgtccatt tatgtaaaaa atcctgctga
     2041 ccttgtttat gtcttgtcag tcaccatttg caaaaccata tttgaccctc aaagaggctg
     2101 aatttgataa gcaacttgct acatactcat aataaggagc taaatagaac acgaatggga
     2161 aatactcaaa tgccaaacta aagaagatat tggccaaaat aaacgctata ccgagagaga
     2221 aacttgattt ttcaacttcc taaaacagtg ttgttcaaac atttctactt atttgtactt
     2281 accagttgaa cctacgtttc cctaataaaa tgtctatggt aaaaagttaa aaaatcctcc
     2341 tacttttgtt agatatattt ttttgtgtaa ttttgtaatc gttatgcggc agtaataata
     2401 tacatattaa tacgagttag gaatcctgta gttctcatat gctacgagga ggtattaaaa
     2461 ggtgcgtttc gacaatgcat ctattgtagt atattattgc ttaatccaaa tgaatattat
     2521 aaatttagga attcttgctc acattgatgc aggaaaaact tccgtaaccg agaatctgct
     2581 gtttgccagt ggagcaacgg aaaagtgcgg ctgtgtggat aatggtgaca ccataacgga
     2641 ctctatggat atagagaaac gtagaggaat tactgttcgg gcttctacga catctattat
     2701 ctggaatggt gtgaaatgca atatcattga cactccggga cacatggatt ttattgcgga
     2761 agtggagcgg acattcaaaa tgcttgatgg agcagtcctc atcttatccg caaaggaagg
     2821 catacaagcg cagacaaagt tgctgttcaa tactttacag aagctgcaaa tcccgacaat
     2881 tatatttatc aataagattg accgagccgg tgtgaatttg gagcgtttgt atctggatat
     2941 aaaagcaaat ctgtctcaag atgtcctgtt tatgcaaaat gttgtcgatg gatcggttta
     3001 tccggtttgc tcccaaacat atataaagga agaatacaaa gaatttgtat gcaaccatga
     3061 cgacaatata ttagaacgat atttggcgga tagcgaaatt tcaccggctg attattggaa
     3121 tacgataatc gctcttgtgg caaaagccaa agtctatccg gtgctacatg gatcagcaat
     3181 gttcaatatc ggtatcaatg agttgttgga cgccatcact tcttttatac ttcctccggc
     3241 atcggtctca aacagacttt catcttatct ttataagata gagcatgacc ccaaaggaca
     3301 taaaagaagt tttctaaaaa taattgacgg aagtctgaga cttcgagacg ttgtaagaat
     3361 caacgattcg gaaaaattca tcaagattaa aaatctaaaa actatcaatc agggcagaga
     3421 gataaatgtt gatgaagtgg gcgccaatga tatcgcgatt gtagaggata tggatgattt
     3481 tcgaatcgga aattatttag gtgctgaacc ttgtttgatt caaggattat cgcatcagca
     3541 tcccgctctc aaatcctccg tccggccaga caggcccgaa gagagaagca aggtgatatc
     3601 cgctctgaat acattgtgga ttgaagaccc gtctttgtcc ttttccataa actcatatag
     3661 tgatgaattg gaaatctcgt tatatggttt aacccaaaag gaaatcatac agacattgct
     3721 ggaagaacga ttttccgtaa aggtccattt tgatgagatc aagactatat acaaagaacg
     3781 acctgtaaaa aaggtcaata agattattca gatcgaagtg ccgcccaacc cttattgggc
     3841 cacaataggg ctgactcttg aacccttacc gttagggaca gggttgcaaa tcgaaagtga
     3901 catctcctat ggttatctga accattcttt tcaaaatgcc gtttttgaag ggattcgtat
     3961 gtcttgccaa tccgggttac atggatggga agtgactgat ctgaaagtaa cttttactca
     4021 agccgagtat tatagcccgg taagtacacc tgctgatttc agacagctga ccccttatgt
     4081 cttcaggctg gccttgcaac agtcaggtgt ggacattctc gaaccgatgc tctattttga
     4141 gttgcagata ccccaagcgg caagttccaa agctattaca gatttgcaaa aaatgatgtc
     4201 tgagattgaa gacatcagtt gcaataatga gtggtgtcat attaaaggga aagttccatt
     4261 aaatacaagt aaagactatg catcagaagt aagttcatac actaagggct taggcatttt
     4321 tatggttaag ccatgcgggt atcaaataac aaaaggcggt tattctgata atatccgcat
     4381 gaacgaaaaa gataaacttt tattcatgtt ccaaaaatca atgtcatcaa aataatggag
     4441 cggtcaggaa atttctataa ggcaatacag ttgggatata tacttatctc cattcttatc
     4501 ggatgtatgg catataatag cctctatgaa tggcaggaga tagaagcatt agaacttggc
     4561 aataaaaaaa tagacgagct ctatggtgca ctctcagtac aatctgctct gatgccgcat
     4621 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     4681 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     4741 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     4801 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     4861 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4921 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4981 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5041 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5101 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5161 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5221 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5281 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5341 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5401 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5461 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5521 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5581 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5641 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5701 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5761 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5821 attggtaact gtcagaccaa gtttactcat aacgcgtcaa ttcgaggggg atcaattccg
     5881 tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat
     5941 ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca
     6001 ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta
     6061 tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa
     6121 atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga
     6181 agcagggtta tgcagcggaa aacggaattg atccggccac gatgcgtccg gcgtagagga
     6241 tctgaagatc agcagttcaa cctgttgata gtacgtacta agctctcatg tttcacgtac
     6301 taagctctca tgtttaacgt actaagctct catgtttaac gaactaaacc ctcatggcta
     6361 acgtactaag ctctcatggc taacgtacta agctctcatg tttcacgtac taagctctca
     6421 tgtttgaaca ataaaattaa tataaatcag caacttaaat agcctctaag gttttaagtt
     6481 ttataagaaa aaaaagaata tataaggctt ttaaagcttt taaggtttaa cggttgtgga
     6541 caacaagcca gggatgtaac gcactgagaa gcccttagag cctctcaaag caattttgag
     6601 tgacacagga acacttaacg gctgacatgg gaattcccct ccaccgcggt ggctcggtac
     6661 caaagacgaa caataagacg ctgaaaagcg tcttttttcg ttttggtccg ataaagtttg
     6721 gaagataaag ctaaaagttc ttatctttgc agtccgaaat aaagacatat aaaagaaaag
     6781 acaccatgga taagaaatac tcaataggct tagctatcgg cacaaatagc gtcggatggg
     6841 cggtgatcac tgatgaatat aaggttccgt ctaaaaagtt caaggttctg ggaaatacag
     6901 accgccacag tatcaaaaaa aatcttatag gggctctttt atttgacagt ggagagacag
     6961 cggaagcgac tcgtctcaaa cggacagctc gtagaaggta tacacgtcgg aagaatcgta
     7021 tttgttatct acaggagatt ttttcaaatg agatggcgaa agtagatgat agtttctttc
     7081 atcgacttga agagtctttt ttggtggaag aagacaagaa gcatgaacgt catcctattt
     7141 ttggaaatat agtagatgaa gttgcttatc atgagaaata tccaactatc tatcatctgc
     7201 gaaaaaaatt ggtagattct actgataaag cggatttgcg cttaatctat ttggccttag
     7261 cgcatatgat taagtttcgt ggtcattttt tgattgaggg agatttaaat cctgataata
     7321 gtgatgtgga caaactattt atccagttgg tacaaaccta caatcaatta tttgaagaaa
     7381 accctattaa cgcaagtgga gtagatgcta aagcgattct ttctgcacga ttgagtaaat
     7441 caagacgatt agaaaatctc attgctcagc tccccggtga gaagaaaaat ggcttatttg
     7501 ggaatctcat tgctttgtca ttgggtttga cccctaattt taaatcaaat tttgatttgg
     7561 cagaagatgc taaattacag ctttcaaaag atacttacga tgatgattta gataatttat
     7621 tggcgcaaat tggagatcaa tatgctgatt tgtttttggc agctaagaat ttatcagatg
     7681 ctattttact ttcagatatc ctaagagtaa atactgaaat aactaaggct cccctatcag
     7741 cttcaatgat taaacgctac gatgaacatc atcaagactt gactctttta aaagctttag
     7801 ttcgacaaca acttccagaa aagtataaag aaatcttttt tgatcaatca aaaaacggat
     7861 atgcaggtta tattgatggg ggagctagcc aagaagaatt ttataaattt atcaaaccaa
     7921 ttttagaaaa aatggatggt actgaggaat tattggtgaa actaaatcgt gaagatttgc
     7981 tgcgcaagca acggaccttt gacaacggct ctattcccca tcaaattcac ttgggtgagc
     8041 tgcatgctat tttgagaaga caagaagact tttatccatt tttaaaagac aatcgtgaga
     8101 agattgaaaa aatcttgact tttcgaattc cttattatgt tggtccattg gcgcgtggca
     8161 atagtcgttt tgcatggatg actcggaagt ctgaagaaac aattacccca tggaattttg
     8221 aagaagttgt cgataaaggt gcttcagctc aatcatttat tgaacgcatg acaaactttg
     8281 ataaaaatct tccaaatgaa aaagtactac caaaacatag tttgctttat gagtatttta
     8341 cggtttataa cgaattgaca aaggtcaaat atgttactga aggaatgcga aaaccagcat
     8401 ttctttcagg tgaacagaag aaagccattg ttgatttact cttcaaaaca aatcgaaaag
     8461 taaccgttaa gcaattaaaa gaagattatt tcaaaaaaat agaatgtttt gatagtgttg
     8521 aaatttcagg agttgaagat agatttaatg cttcattagg tacctaccat gatttgctaa
     8581 aaattattaa agataaagat tttttggata atgaagaaaa tgaagatatc ttagaggata
     8641 ttgttttaac attgacctta tttgaagata gggagatgat tgaggaaaga cttaaaacat
     8701 atgctcacct ctttgatgat aaggtgatga aacagcttaa acgtcgccgt tatactggtt
     8761 ggggacgttt gtctcgaaaa ttgattaatg gtattaggga taagcaatct ggcaaaacaa
     8821 tattagattt tttgaaatca gatggttttg ccaatcgcaa ttttatgcag ctgatccatg
     8881 atgatagttt gacatttaaa gaagacattc aaaaagcaca agtgtctgga caaggcgata
     8941 gtttacatga acatattgca aatttagctg gtagccctgc tattaaaaaa ggtattttac
     9001 agactgtaaa agttgttgat gaattggtca aagtaatggg gcggcataag ccagaaaata
     9061 tcgttattga aatggcacgt gaaaatcaga caactcaaaa gggccagaaa aattcgcgag
     9121 agcgtatgaa acgaatcgaa gaaggtatca aagaattagg aagtcagatt cttaaagagc
     9181 atcctgttga aaatactcaa ttgcaaaatg aaaagctcta tctctattat ctccaaaatg
     9241 gaagagacat gtatgtggac caagaattag atattaatcg tttaagtgat tatgatgtcg
     9301 atgccattgt tccacaaagt ttccttaaag acgattcaat agacaataag gtcttaacgc
     9361 gttctgataa aaatcgtggt aaatcggata acgttccaag tgaagaagta gtcaaaaaga
     9421 tgaaaaacta ttggagacaa cttctaaacg ccaagttaat cactcaacgt aagtttgata
     9481 atttaacgaa agctgaacgt ggaggtttga gtgaacttga taaagctggt tttatcaaac
     9541 gccaattggt tgaaactcgc caaatcacta agcatgtggc acaaattttg gatagtcgca
     9601 tgaatactaa atacgatgaa aatgataaac ttattcgaga ggttaaagtg attaccttaa
     9661 aatctaaatt agtttctgac ttccgaaaag atttccaatt ctataaagta cgtgagatta
     9721 acaattacca tcatgcccat gatgcgtatc taaatgccgt cgttggaact gctttgatta
     9781 agaaatatcc aaaacttgaa tcggagtttg tctatggtga ttataaagtt tatgatgttc
     9841 gtaaaatgat tgctaagtct gagcaagaaa taggcaaagc aaccgcaaaa tatttctttt
     9901 actctaatat catgaacttc ttcaaaacag aaattacact tgcaaatgga gagattcgca
     9961 aacgccctct aatcgaaact aatggggaaa ctggagaaat tgtctgggat aaagggcgag
    10021 attttgccac agtgcgcaaa gtattgtcca tgccccaagt caatattgtc aagaaaacag
    10081 aagtacagac aggcgaattc tccaaggagt caattttacc aaaaagaaat tcggacaagc
    10141 ttattgctcg taaaaaagac tgggatccaa aaaaatatgg tggttttgat agtccaacgg
    10201 tagcttattc agtcctagtg gttgctaagg tggaaaaagg gaaatcgaag aagttaaaat
    10261 ccgttaaaga gttactaggg atcacaatta tggaaagaag ttcctttgaa aaaaatccga
    10321 ttgacttttt agaagctaaa ggatataagg aagttaaaaa agacttaatc attaaactac
    10381 ctaaatatag tctttttgag ttagaaaacg gtcgtaaacg gatgctggct agtgccggag
    10441 aattacaaaa aggaaatgag ctggctctgc caagcaaata tgtgaatttt ttatatttag
    10501 ctagtcatta tgaaaagttg aagggtagtc cagaagataa cgaacaaaaa caattgtttg
    10561 tggagcagca taagcattat ttagatgaga ttattgagca aatcagtgaa ttttctaagc
    10621 gtgttatttt agcagatgcc aatttagata aagttcttag tgcatataac aaacatagag
    10681 acaaaccaat acgtgaacaa gcagaaaata ttattcattt atttacgttg acgaatcttg
    10741 gagctcccgc tgcttttaaa tattttgata caacaattga tcgtaaacga tatacgtcta
    10801 caaaagaagt tttagatgcc actcttatcc atcaatccat cactggtctt tatgaaacac
    10861 gcattgattt gagtcagcta ggaggtgact gatgctgtca taggccggtt gtccggtagt
    10921 ccggatgatt taaggaagat ttctcttctt ttttcacttt ctccgaacaa cctttctttt
    10981 aaaattgtta tttttgtagt gattttaata acctaaaatg aatacgatcg tggtgaatgt
    11041 gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc
    11101 ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc
    11161 gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc
    11221 gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc
    11281 ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg
    11341 aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg
    11401 gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac
    11461 taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt
    11521 ctcccatgag gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca
    11581 aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg
    11641 gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag
    11701 tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc
    11761 gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg
    11821 gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgagg acagctcatg
    11881 ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt
    11941 ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt
    12001 ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc
    12061 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    12121 aaaagaaaag gcgttttgtt tttcttcttt accttctttc cctttcgcta agagagtctg
    12181 agaaacgata gaaaaagaaa agcgaaaaaa cttccgaaaa catttggtag ttaaaataaa
    12241 acctcttacc tttgcacccg cgcattttaa aataaaataa attatttatg atattaaacg
    12301 aatatgtcta gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag
    12361 gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct
    12421 acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg
    12481 ttagataggc accatactca cttttgccct ttagaagggg aaagctggca agatttttta
    12541 cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta
    12601 catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt
    12661 ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat
    12721 tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa
    12781 acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac
    12841 caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa
    12901 caacttaaat gtgaaagtgg gtcttaatga actgcacttg ctttgataat taatgataaa
    12961 caatctaaaa gcactctaat cgttatcgga gtgcttttag attactaatc aaattgctt
//
