LOCUS       Exported                6922 bp ds-DNA     circular SYN 27-JUN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6922)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 20, 2020 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6922
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..30
                     /label=mmD661
     misc_feature    1
                     /label=origin
     misc_recomb     118..2262
                     /label=NluI fragment from pKNOCK
                     /note="NluI fragment from pKNOCK"
     CDS             complement(156..890)
                     /label=ermG
                     /note="ermG"
     CDS             1378..2239
                     /label=beta-lactamase from pBR322
                     /note="beta-lactamase from pBR322"
     primer_bind     1837..1856
                     /label=oMT203
     primer_bind     complement(2089..2106)
                     /label=oMT1189
     primer_bind     2089..2106
                     /label=oMT1184
     misc_feature    2097
                     /label=t2096a
     misc_recomb     2277..3062
                     /label=RP4 oriT/RSK oriV
                     /note="RP4 oriT/RSK oriV"
     primer_bind     complement(2340..2359)
                     /label=oMT204
     primer_bind     2968..2987
                     /label=pNBU1_seq.FOR
     misc_feature    3062
                     /label=3062 mutation from ""g"" to ""t""
                     /note="Mutated in pMT1fix2, but will not fix, since it's at
                     the backbone of the vector and is not CDS or a region with 
                     important UTR."
     misc_feature    3063..3122
                     /label=pNBU2-Lac-dCas9-NL3 PCR Product - pNBU2-Lac-dCas9-
                     /note="Geneious type: Inserted sequence"
     terminator      3078..3107
                     /label=tL17 terminator
                     /label=tL17
     primer_bind     complement(3104..3134)
                     /label=oMT1185
     primer_bind     3123..3267
                     /label=oMT1342
     misc_feature    3123..3130
                     /label=L3S3P21
     misc_feature    3130..3133
                     /label=CGCT
     misc_feature    3131..4899
                     /label=Amp up this region
     promoter        3134..3176
                     /label=cfxA_promoter
     promoter        3134..3135
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     promoter        3134..3135
                     /label=PcfxA promoter
                     /label=PcfxA
     misc_feature    3159..3163
                     /label=-60
     misc_feature    3170..3174
                     /label=-50
     misc_feature    3177..3185
                     /label=AANGTANAC
     misc_feature    3186..3191
                     /label=N6
     misc_feature    3189..3192
                     /label=-33
     misc_feature    3192..3200
                     /label=GTNTACNTT
     -35_signal      3193..3198
                     /label=-35
     promoter        3201..3212
                     /label=PcfxA
     misc_feature    3213..3220
                     /label=-7
     misc_feature    3225
                     /label=+1
     misc_feature    3226..3234
                     /label=AANGTANAC
     misc_feature    3235..3240
                     /label=N6
     misc_feature    3241..3249
                     /label=GTNTACNTT
     -35_signal      3242..3247
                     /label=-35
     misc_feature    3250..3324
                     /label=RiboJ00
     terminator      3251..3252
                     /label=rpiL*
                     /note="rpiL* termninator"
     terminator      3325..3367
                     /label=rpiL*
                     /note="rpiL* termninator"
     primer_bind     complement(3363..3392)
                     /label=oMT641
     CDS             3368..3883
                     /codon_start=1
                     /label=Luciferase Reporter
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     3368..3392
                     /label=oMT41
     primer_bind     3656..3675
                     /label=oMT399
     primer_bind     complement(3862..3887)
                     /label=oMT1343
     primer_bind     3884..3994
                     /label=oMT1344
     misc_feature    3884..3968
                     /label=Amp up this region
     misc_feature    3884..3887
                     /label=TGAA
     misc_feature    3891..3961
                     /label=L3S2P56
     misc_feature    3954..3955
                     /label=Restriction Sites
     promoter        3969..4251
                     /label=BT1311_promoter
     -35_signal      4212..4215
                     /label=-33
     -10_signal      4238..4245
                     /label=-7
     RBS             4252..4277
                     /label=BT1311_RBS
     CDS             4278..4889
                     /codon_start=1
                     /label=VFA0359-Bt
                     /translation="MQKKLTRSQQKHLDIINAAKEEFIEFGFLAANMDRITSSAEVSKR
                     TLYRHFESKEVLFESVLTIINDSVNESISYHFDPNKSTEEQLTEIAYKEIDVLYKTYGI
                     ALARTIVMEFLRQPEMAKTLIQNIYSIRAITQWFRSAIEAKRLKDADPKLMTDVYVSLF
                     QGLFFWPQVMHLDLEPHGEELSQKIETLTTIFLQSYGVAE"
     CDS             4278..4280
                     /codon_start=1
                     /label=CmeR(NCTC11168)
                     /translation="M"
     gene            4278..4280
                     /gene="rpoD"
                     /gene_synonym="alt; ECK3057; JW3039"
                     /locus_tag="b3067"
                     /label=rpoD
                     /db_xref="EcoGene:EG10896"
                     /db_xref="GeneID:947567"
     CDS             4278..4280
                     /codon_start=1
                     /label=BreR-Bt
                     /translation="M"
     primer_bind     4547..4568
                     /label=internal seq primer
     primer_bind     complement(4867..4894)
                     /label=oMT1309
     CDS             4887..4889
                     /codon_start=1
                     /label=CmeR(NCTC11168)
                     /translation=""
     gene            4887..4889
                     /gene="rpoD"
                     /gene_synonym="alt; ECK3057; JW3039"
                     /locus_tag="b3067"
                     /label=rpoD
                     /db_xref="EcoGene:EG10896"
                     /db_xref="GeneID:947567"
     CDS             4887..4889
                     /codon_start=1
                     /label=BreR-Bt
                     /translation=""
     CDS             4887..4889
                     /label=CmeR(NCTC11168)
     gene            4887..4889
                     /label=rpoD
     CDS             4887..4889
                     /label=BreR-Bt
     primer_bind     4890..4914
                     /label=oMT1188
     misc_feature    4890..4893
                     /label=CAAT
     misc_feature    4900..4905
                     /label=problematic assembly region
     terminator      4906..4966
                     /label=L3S2P21 Terminator
     primer_bind     complement(4945..4985)
                     /label=oMT1272
     misc_feature    4945..4966
                     /label=Feature 67
     primer_bind     4967..4994
                     /label=oMT1259
     primer_bind     complement(5092..5112)
                     /label=pNBU1_seq.REV
     misc_feature    complement(5141..5179)
                     /label=mmD660
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    5180..6922
                     /label=intN1 Construct PCR Product - intN1 Construct P
                     /label=intN1 Construct PCR Product - intN1 Construct PCR 
                     Product 2
                     /note="Geneious type: Inserted sequence"
     misc_feature    5180..5979
                     /label=Piece1
     misc_feature    5180..5209
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
     primer_bind     5180..5208
                     /label=mmD658
     primer_bind     5201..5221
                     /label=oMT113
     misc_feature    5210..5539
                     /label=attN1 Region
     primer_bind     5210..5229
                     /label=intN1 Construct PCR Product (fwd) - pNBU2-PcfxA
                     /label=intN1 Construct PCR Product (fwd) - 
                     pNBU2-PcfxA-NanoLuc PCR Product
     misc_feature    5260..5275
                     /label=DR1
     misc_feature    5293..5307
                     /label=IR1
     misc_feature    5317..5331
                     /label=IR2
     misc_feature    5337..5350
                     /label=CC Region
     misc_feature    5349
                     /label=G-->C
     misc_feature    5349
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     misc_feature    5350
                     /label='-1'
     misc_feature    5351
                     /label='+1'
     misc_feature    5368..5383
                     /label=DR2
     primer_bind     complement(5406..5428)
                     /label=oMT114
     misc_feature    5492..5508
                     /label=DR3
     CDS             5555..5979
                     /codon_start=1
                     /transl_table=11
                     /gene="intN1"
                     /product="IntN1"
                     /label=intN1
                     /label=intN1 CDS
                     /note="NBU1 integrase"
                     /db_xref="GI:8308020"
                     /protein_id="AAF74437.1"
                     /translation="MKVTFIIKKAAKRYDTESMATIYVRFRNGRQLDSVAPTQLAINPN
                     LWDDKDECVKTKAVCNEEMRTHINEEIRQLKTYIEKVYQQEKEAIDKEWLKTTLDKFYH
                     PEKYFLPEEVVIKPTIGELFDEFLNKHPLSEVRKKNF"
     gene            5555..5979
                     /gene="intN1"
                     /label=intN1
                     /label=intN1 gene
     primer_bind     complement(5771..5798)
                     /label=oMT157
     primer_bind     5771..5798
                     /label=oMT156
     misc_feature    complement(5949..5979)
                     /label=mmD659
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    5950..6922
                     /label=Piece2
     CDS             5950..6892
                     /codon_start=1
                     /transl_table=11
                     /gene="intN1"
                     /product="IntN1"
                     /label=intN1
                     /label=intN1 CDS
                     /note="NBU1 integrase"
                     /db_xref="GI:8308020"
                     /protein_id="AAF74437.1"
                     /translation="MVGSTKEKFPGCQKSLTAL*TICKGYKERTKGLYP*CGFGNT*HA
                     SGYVGFLSERIPVL*TLPEHL*SHSRKEDTTAQKQKHADRLFFKNTHILPVVLR*QTHH
                     KQTFRQVSDRGVYIWYTLLYNTRRKGQDF*CRPFCHPTTGNTEGYIHISDTDRMQGERP
                     VPNDQTKCGQ*SHRIYSQENQRGESGYGTCST*RQSERNP*TLQRI*GKTVAVHIRAKV
                     Q*CHKKDIQISWS*PHRNNLRPVDAQRNQTTYL*SGKQPSGKTYVYRQYL*KSERPEPC
                     FRTVGTQGGKQSFQTIQGY*RRNEERPCKTTGL"
     gene            5950..6892
                     /gene="intN1"
                     /label=intN1
                     /label=intN1 gene
     misc_feature    5950..5979
                     /label=Piece2
     misc_feature    5950..5979
                     /label=Ligation
     primer_bind     5950..5969
                     /label=intN1 Construct PCR Product 2 (fwd)
     misc_feature    complement(5955..5979)
                     /label=intN1 Construct PCR Product (rev)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(6868..6894)
                     /label=intN1 Construct PCR Product 2 (rev) - pNBU2-Pcf
                     /label=intN1 Construct PCR Product 2 (rev) - 
                     pNBU2-PcfxA-NanoLuc PCR Product
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    6884
                     /label=T
                     /note="Geneious type: Editing History Replacement"
     misc_feature    complement(6886..6922)
                     /label=mmD661
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    6886
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    6893..6922
                     /label=HOMOLOGY
     misc_feature    6895..6922
                     /label=CTTGTAGTTCGTTTAATTCGCTTTTCGTTTTTACGAATTTAACGAAT
                     /label=CTTGTAGTTCGTTTAATTCGCTTTTCGTTTTTACGAATTTAACGAATTGA 
                     ATATCATTGTTTTACGATTGATTTTCATTATATATTTGCGCCACTATCCTAATCGGGG 
                     TAGTGGCTTTTCGTTTATAATACTGCCGAAACGGGAA
                     /note="Geneious type: Editing History Replacement"
ORIGIN
        1 agagtagcga tattaaaaga atccgatgag aaaagactaa tatttatcta tccattcagt
       61 ttgatttttc aggactttac atcgtcctga aagtatttgt tggtaccggt accgaggacg
      121 cgtaaacatt tacagttgca tgtggcctat tgtttttagc cgttaaatat tttataacta
      181 ttaaatagcg atacaaattg ttcgaaacta atattgttta tatcatatat tctcgcatgt
      241 tttaaagctt tattaaattg attttttgta aacagttttt cgtactcttt gttaacccat
      301 ttcattacaa aagtttcata tttttttctc tctttaaatg ccatttttgc tggctttctt
      361 tttaatacaa ttaatgtgct atccacttta ggttttggat ggaaataata cctaggaatt
      421 tttgctaata tagaaatatc tacctctgcc attaacagca atgctagtga tctgtttgta
      481 tctaataaca ttttagcaaa accatattcc actattaaat aacttattgt ggctgaactt
      541 tcaaaaacaa tttttcgaat tatatttgtg cttatgttgt aaggtatgct gccaaatatt
      601 ttatatggat tgtggctagg aaatgtaaat ttcagtatat catcatttac tatttgatag
      661 ttaggataat ttaagagctt attacgagtt acctcacata atttagaatc aatttctatc
      721 gccgttacaa aattacatct ctttaccaat ccagcagtaa aatgaccttt ccctgcacct
      781 atttcaaaga tgttatcttt ttcatctaaa cttatgcaat tcattatttt ttctatgtga
      841 tattttgaag taataaaatt ttgactatct tttatattta ctttgttcat tataacctct
      901 ccttaattta ttgcatctct tttcgaatat ttatgttttt tgagaaaaga acgtactcat
      961 ggttcatccc gatatgcgta tcggtctgta tatcagcaac tttctatgtg tttcaactac
     1021 aatagtcatc tattctcatc tttctgagtc caccccctgc aaagcccctc tttacgacat
     1081 aaaaattcgg tcggaaaagg tatgcaaaag atgtttctct ctttaagaga aactcttcgg
     1141 gatgcaaaaa tatgaaaata actccaattc accaaattat atagcgactt ttttacaaaa
     1201 tgctaaaatt tgttgatttc cgtcaagcaa ttgttgagca aaaatgtctt ttacgataaa
     1261 atgatacctc aatatcaact gtttagcaaa acgatatttc tcttaaagag agaaacacct
     1321 ttttgttcac caatccccga cttttaatcc cgcggccatg attgaaaaag gaagagtatg
     1381 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1441 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     1501 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     1561 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     1621 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     1681 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     1741 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     1801 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     1861 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     1921 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     1981 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2041 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtcacgc
     2101 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2161 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     2221 ctgattaagc attggtaact gtcagaccaa gtttactcat aacgcgtcaa ttcgaggggg
     2281 atcaattccg tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc
     2341 ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg
     2401 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct
     2461 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
     2521 ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata
     2581 atgaccccga agcagggtta tgcagcggaa aacggaattg atccggccac gatgcgtccg
     2641 gcgtagagga tctgaagatc agcagttcaa cctgttgata gtacgtacta agctctcatg
     2701 tttcacgtac taagctctca tgtttaacgt actaagctct catgtttaac gaactaaacc
     2761 ctcatggcta acgtactaag ctctcatggc taacgtacta agctctcatg tttcacgtac
     2821 taagctctca tgtttgaaca ataaaattaa tataaatcag caacttaaat agcctctaag
     2881 gttttaagtt ttataagaaa aaaaagaata tataaggctt ttaaagcttt taaggtttaa
     2941 cggttgtgga caacaagcca gggatgtaac gcactgagaa gcccttagag cctctcaaag
     3001 caattttgag tgacacagga acacttaacg gctgacatgg gaattcccct ccaccgcggt
     3061 ggattaatgc ggctgcaatt tttttgggcg gggccgccca aaaaaatcct agcaccctgc
     3121 agggtctccc gctttacaaa gaaaattcga caaactgtta tttttctatc tatttaaatg
     3181 tacacccgtt tgtttacttt actttagtta tgtacctttg tcggcaatgt acacccgaaa
     3241 gtttacttta gctgtcaccg gatgtgcttt ccggtctgat gagtccgtga ggacgaaaca
     3301 gcctctacaa ataattttgt ttaacgcatt ttaaaataaa ataaattatt tatttaatta
     3361 aacgaatatg gtttttactc tggaagattt tgttggcgat tggcgtcaga ccgcgggtta
     3421 taatttggat caagtcctgg aacagggtgg cgtaagctct ctgttccaga acctgggtgt
     3481 gagcgtgacg ccgattcagc gcatcgttct gtccggcgag aacggtctga aaattgatat
     3541 tcatgtgatc atcccgtacg aaggcctgag cggtgaccaa atgggtcaaa tcgagaaaat
     3601 ctttaaagtc gtctacccag ttgacgatca ccacttcaag gttatcttgc attacggtac
     3661 gctggtgatt gatggtgtga ccccgaatat gattgactat ttcggccgtc cgtatgaagg
     3721 cattgccgtt tttgacggta aaaagatcac cgtcaccggt accctgtgga atggcaataa
     3781 gattattgac gagcgtctga ttaacccgga cggcagcctg ctgttccgcg tgaccatcaa
     3841 cggtgtcacg ggttggcgtc tgtgcgagcg catcctggca taatgaactg ggatccctcg
     3901 gtaccaaatt ttcgaaaaaa gacgctgaaa agcgtctttt ttcgttttgg tccgcggccg
     3961 ccggccgctg atctggaaga agcaatgaaa gctgctgtta agtctccgaa tcaggtattg
     4021 ttcctgacag gtgtattccc atccggtaaa cgcggatact ttgcagttga tctgactcag
     4081 gaataaatta taaattaagg taagaagatt gtaggataag ctaatgaaat agaaaaagga
     4141 tgccgtcaca caacttgtcg gcattctttt ttgttttatt agttgaaaat atagtgaaaa
     4201 agttgcctaa atatgtatgt taacaaatta tttgtcgtaa ctttgcactc caaatctgtt
     4261 tttaacatat ggcactaatg cagaaaaaat tgactcgttc tcagcagaaa cacttggaca
     4321 tcatcaacgc tgctaaagaa gaattcatcg aattcggttt cttggctgct aacatggacc
     4381 gtatcacttc ttctgctgaa gtatctaaac gtactttgta ccgtcacttc gaatctaaag
     4441 aagtattgtt cgaatctgta ttgactatca tcaacgactc tgtaaacgaa tctatctctt
     4501 accacttcga cccgaacaaa tctactgaag aacagttgac tgaaatcgct tacaaagaaa
     4561 tcgacgtatt gtacaaaact tacggtatcg ctttggctcg tactatcgta atggaattct
     4621 tgcgtcagcc ggaaatggct aaaactttga tccagaacat ctactctatc cgtgctatca
     4681 ctcagtggtt ccgttctgct atcgaagcta aacgtttgaa agacgctgac ccgaaattga
     4741 tgactgacgt atacgtatct ttgttccagg gtttgttctt ctggccgcag gtaatgcact
     4801 tggacttgga accgcacggt gaagaattgt ctcagaaaat cgaaactttg actactatct
     4861 tcttgcagtc ttacggtgta gctgaataac aatgaatggg ctttcctcgg taccaaattc
     4921 cagaaaagag gcctcccgaa aggggggcct tttttcgttt tggtccctaa ttgcctatct
     4981 tccagtgatg gaacagcatt tgtgcattgg ctgcaacaat cagccttact tgtgcctgtt
     5041 ctatttccga accgaccgct tgtatgaatc catcaaaatt cgttttctct atgttggatt
     5101 ccttgttgct catattgtga tgataatttc tacaaatata gtcattggta actatctatg
     5161 aaactgtttg atacttttac tcatcggatt cttttaatat cgctactctc tacgttcaac
     5221 caaaagaaat aatgacttac tgctatattt tttgcacgtg tggggaaaat gtggggaaaa
     5281 ttcaagcaaa agaaaaagct aagtattgaa ctatcaaata cttagctttc tttcttgtac
     5341 ccagaccccg catttgaaat aattaaagtg gggaaaatgt gggtaaaaag aaaaatgcgg
     5401 aaaaacgcca caattacact gtatttcaat atgttataat cctattaaat tttaatccaa
     5461 gtttaatcga attgcaaaat atttagcaga tgtggggaaa atgctgggga aaatatttat
     5521 atttgcagca gagtaaaatt caacctgaaa gtctatgaaa gtaaccttta tcattaaaaa
     5581 agcagccaaa cgatatgata cagaatccat ggctacaatc tatgtccgtt ttagaaacgg
     5641 aaggcagtta gactccgttg ctcctactca gttagccatc aatcccaatc tatgggatga
     5701 taaagacgaa tgtgtaaaaa cgaaagctgt ctgcaatgaa gaaatgcgta cccatataaa
     5761 tgaagagata cgccagttga aaacctatat cgagaaggta tatcaacaag aaaaggaagc
     5821 aatagacaaa gaatggctaa aaacaacact tgataaattt taccatcctg aaaaatattt
     5881 tttgccggag gaagtggtta tcaagcctac cattggagaa ctattcgatg aatttctaaa
     5941 caagcaccct ttgtcggaag tacgaaagaa aaatttccgg gttgtcaaaa gagccttact
     6001 gcgttatgaa ctatatgtaa gggctacaaa gagaggacaa aagggcttta tccttgatgt
     6061 ggatttggta acacctgaca cgcttcggga tatgtgggat ttctttcaga acgaatacca
     6121 gtattatgaa ctttacccga gcatttatga agccattccc gaaaagagga caccacagcc
     6181 cagaagcaaa aacacgctga tagactgttt ttcaagaata cgcacattct tcctgtggtg
     6241 cttcgataac aaacgcacca caaacagacc tttcgacaag tttccgatag aggagtgtac
     6301 atatggtaca ccttattata taacactcga agaaagggac aggattttta atgcagacct
     6361 ttctgccacc ccacaactgg caatacagag ggatatattc atatttcaga cactgatagg
     6421 atgcagggtg agcgacctgt accgaatgac caaactaaat gtggtcaatg aagccataga
     6481 atatattccc aagaaaacca aagaggggaa tccggttacg gtacgtgttc cacttaacga
     6541 caaagcgaaa gaaatccttg aacgctacaa agaatatgag ggaaaactgt tgccgttcat
     6601 atccgagcaa aagtacaatg atgccataaa aaagatattc aaattagctg gagttgaccg
     6661 catcgtaaca atcttagacc cgttgacgca caacgaaatc aaacgaccta tttatgaagt
     6721 ggcaagcagc catctggcaa gacgtacgtt tatcggcaat atctataaaa aagtgaaaga
     6781 cccgaacctt gtttccgcac tgtcgggaca caaggaggga agcaaagctt tcagacgata
     6841 cagggatatt gacgaagaaa tgaagaaaga ccttgtaaaa ctactggact gataaaagta
     6901 tcaaacagtt tcatagatag tt
//
