LOCUS       Exported                7194 bp ds-DNA     circular SYN 11-SEP-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7194)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 20, 2020 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7194
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..30
                     /label=mmD661
     misc_feature    1
                     /label=origin
     misc_recomb     118..2262
                     /label=NluI fragment from pKNOCK
                     /note="NluI fragment from pKNOCK"
     CDS             complement(156..890)
                     /label=ermG
                     /note="ermG"
     CDS             1378..2239
                     /label=beta-lactamase from pBR322
                     /note="beta-lactamase from pBR322"
     primer_bind     1837..1856
                     /label=oMT203
     primer_bind     2089..2106
                     /label=oMT1184
     primer_bind     complement(2089..2106)
                     /label=oMT1189
     misc_feature    2097
                     /label=t2096a
     misc_recomb     2277..3062
                     /label=RP4 oriT/RSK oriV
                     /note="RP4 oriT/RSK oriV"
     primer_bind     complement(2340..2359)
                     /label=oMT204
     primer_bind     2968..2987
                     /label=pNBU1_seq.FOR
     misc_feature    3062
                     /label=3062 mutation from ""g"" to ""t""
                     /note="Mutated in pMT1fix2, but will not fix, since it's at
                     the backbone of the vector and is not CDS or a region with 
                     important UTR."
     misc_feature    3063..3122
                     /label=pNBU2-Lac-dCas9-NL3 PCR Product - pNBU2-Lac-dCas9-
                     /note="Geneious type: Inserted sequence"
     terminator      3078..3107
                     /label=tL17 terminator
                     /label=tL17
     primer_bind     3123..3159
                     /label=oMT1293
     misc_feature    3123..3129
                     /label=Scar
     misc_feature    3130..3133
                     /label=CGCT
     promoter        3134..3176
                     /label=cfxA_promoter
     misc_feature    3177..3185
                     /label=AANGTANAC
     misc_feature    3186..3191
                     /label=N6
     misc_feature    3189..3192
                     /label=-33
     misc_feature    3192..3200
                     /label=GTNTACNTT
     -35_signal      3193..3198
                     /label=-35
     promoter        3201..3212
                     /label=PcfxA
     misc_feature    3213..3220
                     /label=-7
     misc_feature    3222..3225
                     /label=4bp-2
     primer_bind     complement(3223..3249)
                     /label=oMT916
     misc_feature    3225
                     /label=+1
     misc_feature    3226..3234
                     /label=AANGTANAC
     misc_feature    3235..3240
                     /label=N6
     misc_feature    3241..3249
                     /label=GTNTACNTT
     -35_signal      3242..3247
                     /label=-35
     primer_bind     3250..3282
                     /label=oMT1375
     misc_feature    3250..3269
                     /label=sgRNA-7
     primer_bind     3269..3303
                     /label=oMT915
     misc_feature    3270..3311
                     /label=chimera tracrRNA
     misc_feature    3316..3352
                     /label=S. pyogenes terminator
     primer_bind     complement(3333..3431)
                     /label=oMT1299
     misc_feature    3353..3402
                     /label=L3S3P41
     misc_feature    3353..3356
                     /label=GGAT
     misc_feature    3354..3358
                     /label=Amp up this region
     primer_bind     complement(3396..3414)
                     /label=oMT1383
     misc_feature    3406..3413
                     /label=Restriction Sites
     primer_bind     3413..3539
                     /label=oMT914
     misc_feature    3414..3489
                     /label=PBF2884
     primer_bind     3414..3433
                     /label=oMT1006
     misc_feature    3456..3460
                     /label=-60
     primer_bind     complement(3464..3494)
                     /label=oMT1351
     primer_bind     complement(3464..3492)
                     /label=oMT1116
     misc_feature    3467..3471
                     /label=-50
     primer_bind     3473..3497
                     /label=oMT1114
     primer_bind     complement(3474..3497)
                     /label=oMT1064
     misc_feature    3486..3489
                     /label=-33
     misc_feature    3495..3496
                     /label=PAM
     misc_feature    3496..3497
                     /label=CATA
     misc_feature    3498..3517
                     /label=sgRNA-7
     misc_feature    3498..3509
                     /label=20bp
     misc_feature    3510..3517
                     /label=-7
     primer_bind     3517..3539
                     /label=oMT1352
     misc_feature    3522..3596
                     /label=RiboJ00
     terminator      3597..3639
                     /label=rpiL*
                     /note="rpiL* termninator"
     CDS             3640..4155
                     /codon_start=1
                     /label=Luciferase Reporter
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     3640..3664
                     /label=oMT41
     primer_bind     complement(3651..3670)
                     /label=oMT386
     primer_bind     3928..3947
                     /label=oMT399
     misc_feature    4127..4155
                     /label=problematic assembly region
     primer_bind     complement(4134..4159)
                     /label=oMT1343
     primer_bind     complement(4134..4155)
                     /label=oMT1347
     primer_bind     complement(4137..4155)
                     /label=oMT1349
     primer_bind     complement(4137..4155)
                     /label=oMT1307
     primer_bind     4156..4266
                     /label=oMT1348
     primer_bind     4156..4266
                     /label=oMT1344
     misc_feature    4156..4159
                     /label=TGAA
     misc_feature    4156..4159
                     /label=Amp up this region
     misc_feature    4160..5161
                     /label=Amp up this region
     misc_feature    4163..4233
                     /label=L3S2P56
     misc_feature    4226..4227
                     /label=Restriction Sites
     promoter        4241..4523
                     /label=BT1311_promoter
     primer_bind     4419..4438
                     /label=oMT1135
     -35_signal      4484..4487
                     /label=-33
     primer_bind     4505..4524
                     /label=oMT100
     -10_signal      4510..4517
                     /label=-7
     RBS             4524..4549
                     /label=BT1311_RBS
     CDS             4550..5161
                     /codon_start=1
                     /label=VFA0359-Bt
                     /translation="MQKKLTRSQQKHLDIINAAKEEFIEFGFLAANMDRITSSAEVSKR
                     TLYRHFESKEVLFESVLTIINDSVNESISYHFDPNKSTEEQLTEIAYKEIDVLYKTYGI
                     ALARTIVMEFLRQPEMAKTLIQNIYSIRAITQWFRSAIEAKRLKDADPKLMTDVYVSLF
                     QGLFFWPQVMHLDLEPHGEELSQKIETLTTIFLQSYGVAE"
     CDS             4550..4552
                     /codon_start=1
                     /label=CmeR(NCTC11168)
                     /translation="M"
     gene            4550..4552
                     /gene="rpoD"
                     /gene_synonym="alt; ECK3057; JW3039"
                     /locus_tag="b3067"
                     /label=rpoD
                     /db_xref="EcoGene:EG10896"
                     /db_xref="GeneID:947567"
     CDS             4550..4552
                     /codon_start=1
                     /label=BreR-Bt
                     /translation="M"
     primer_bind     4819..4840
                     /label=internal seq primer
     primer_bind     4819..4840
                     /label=oMT379
     primer_bind     complement(5132..5156)
                     /label=oMT677
     primer_bind     complement(5139..5166)
                     /label=oMT1309
     CDS             5159..5161
                     /codon_start=1
                     /label=CmeR(NCTC11168)
                     /translation=""
     gene            5159..5161
                     /gene="rpoD"
                     /gene_synonym="alt; ECK3057; JW3039"
                     /locus_tag="b3067"
                     /label=rpoD
                     /db_xref="EcoGene:EG10896"
                     /db_xref="GeneID:947567"
     CDS             5159..5161
                     /codon_start=1
                     /label=BreR-Bt
                     /translation=""
     CDS             5159..5161
                     /label=CmeR(NCTC11168)
     gene            5159..5161
                     /label=rpoD
     CDS             5159..5161
                     /label=BreR-Bt
     primer_bind     5162..5186
                     /label=oMT1188
     misc_feature    5162..5171
                     /label=Amp up this region
     misc_feature    5162..5165
                     /label=CAAT
     primer_bind     5172..5186
                     /label=oMT676
     misc_feature    5172..5177
                     /label=problematic assembly region
     terminator      5178..5238
                     /label=L3S2P21 Terminator
     primer_bind     complement(5217..5257)
                     /label=oMT1272
     misc_feature    5217..5238
                     /label=Feature 67
     primer_bind     5239..5266
                     /label=oMT1259
     primer_bind     complement(5364..5384)
                     /label=pNBU1_seq.REV
     misc_feature    complement(5413..5451)
                     /label=mmD660
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    5452..7194
                     /label=intN1 Construct PCR Product - intN1 Construct P
                     /label=intN1 Construct PCR Product - intN1 Construct PCR 
                     Product 2
                     /note="Geneious type: Inserted sequence"
     misc_feature    5452..6251
                     /label=Piece1
     misc_feature    5452..5481
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
     primer_bind     5452..5480
                     /label=mmD658
     primer_bind     5473..5493
                     /label=oMT113
     misc_feature    5482..5811
                     /label=attN1 Region
     primer_bind     5482..5501
                     /label=intN1 Construct PCR Product (fwd) - pNBU2-PcfxA
                     /label=intN1 Construct PCR Product (fwd) - 
                     pNBU2-PcfxA-NanoLuc PCR Product
     misc_feature    5532..5547
                     /label=DR1
     misc_feature    5565..5579
                     /label=IR1
     misc_feature    5589..5603
                     /label=IR2
     misc_feature    5609..5622
                     /label=CC Region
     misc_feature    5621
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     misc_feature    5621
                     /label=G-->C
     misc_feature    5622
                     /label='-1'
     misc_feature    5623
                     /label='+1'
     misc_feature    5640..5655
                     /label=DR2
     primer_bind     complement(5678..5700)
                     /label=oMT114
     misc_feature    5764..5780
                     /label=DR3
     gene            5827..6251
                     /gene="intN1"
                     /label=intN1
                     /label=intN1 gene
     CDS             5827..6251
                     /codon_start=1
                     /transl_table=11
                     /gene="intN1"
                     /product="IntN1"
                     /label=intN1
                     /label=intN1 CDS
                     /note="NBU1 integrase"
                     /db_xref="GI:8308020"
                     /protein_id="AAF74437.1"
                     /translation="MKVTFIIKKAAKRYDTESMATIYVRFRNGRQLDSVAPTQLAINPN
                     LWDDKDECVKTKAVCNEEMRTHINEEIRQLKTYIEKVYQQEKEAIDKEWLKTTLDKFYH
                     PEKYFLPEEVVIKPTIGELFDEFLNKHPLSEVRKKNF"
     primer_bind     6043..6070
                     /label=oMT156
     primer_bind     complement(6043..6070)
                     /label=oMT157
     misc_feature    complement(6221..6251)
                     /label=mmD659
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    6222..7194
                     /label=Piece2
     gene            6222..7164
                     /gene="intN1"
                     /label=intN1
                     /label=intN1 gene
     CDS             6222..7164
                     /codon_start=1
                     /transl_table=11
                     /gene="intN1"
                     /product="IntN1"
                     /label=intN1
                     /label=intN1 CDS
                     /note="NBU1 integrase"
                     /db_xref="GI:8308020"
                     /protein_id="AAF74437.1"
                     /translation="MVGSTKEKFPGCQKSLTAL*TICKGYKERTKGLYP*CGFGNT*HA
                     SGYVGFLSERIPVL*TLPEHL*SHSRKEDTTAQKQKHADRLFFKNTHILPVVLR*QTHH
                     KQTFRQVSDRGVYIWYTLLYNTRRKGQDF*CRPFCHPTTGNTEGYIHISDTDRMQGERP
                     VPNDQTKCGQ*SHRIYSQENQRGESGYGTCST*RQSERNP*TLQRI*GKTVAVHIRAKV
                     Q*CHKKDIQISWS*PHRNNLRPVDAQRNQTTYL*SGKQPSGKTYVYRQYL*KSERPEPC
                     FRTVGTQGGKQSFQTIQGY*RRNEERPCKTTGL"
     misc_feature    6222..6251
                     /label=Ligation
     misc_feature    6222..6251
                     /label=Piece2
     primer_bind     6222..6241
                     /label=intN1 Construct PCR Product 2 (fwd)
     misc_feature    complement(6227..6251)
                     /label=intN1 Construct PCR Product (rev)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(7140..7166)
                     /label=intN1 Construct PCR Product 2 (rev) - pNBU2-Pcf
                     /label=intN1 Construct PCR Product 2 (rev) - 
                     pNBU2-PcfxA-NanoLuc PCR Product
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    7156
                     /label=T
                     /note="Geneious type: Editing History Replacement"
     misc_feature    complement(7158..7194)
                     /label=mmD661
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    7158
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    7165..7194
                     /label=HOMOLOGY
     misc_feature    7167..7194
                     /label=CTTGTAGTTCGTTTAATTCGCTTTTCGTTTTTACGAATTTAACGAAT
                     /label=CTTGTAGTTCGTTTAATTCGCTTTTCGTTTTTACGAATTTAACGAATTGA 
                     ATATCATTGTTTTACGATTGATTTTCATTATATATTTGCGCCACTATCCTAATCGGGG 
                     TAGTGGCTTTTCGTTTATAATACTGCCGAAACGGGAA
                     /note="Geneious type: Editing History Replacement"
ORIGIN
        1 agagtagcga tattaaaaga atccgatgag aaaagactaa tatttatcta tccattcagt
       61 ttgatttttc aggactttac atcgtcctga aagtatttgt tggtaccggt accgaggacg
      121 cgtaaacatt tacagttgca tgtggcctat tgtttttagc cgttaaatat tttataacta
      181 ttaaatagcg atacaaattg ttcgaaacta atattgttta tatcatatat tctcgcatgt
      241 tttaaagctt tattaaattg attttttgta aacagttttt cgtactcttt gttaacccat
      301 ttcattacaa aagtttcata tttttttctc tctttaaatg ccatttttgc tggctttctt
      361 tttaatacaa ttaatgtgct atccacttta ggttttggat ggaaataata cctaggaatt
      421 tttgctaata tagaaatatc tacctctgcc attaacagca atgctagtga tctgtttgta
      481 tctaataaca ttttagcaaa accatattcc actattaaat aacttattgt ggctgaactt
      541 tcaaaaacaa tttttcgaat tatatttgtg cttatgttgt aaggtatgct gccaaatatt
      601 ttatatggat tgtggctagg aaatgtaaat ttcagtatat catcatttac tatttgatag
      661 ttaggataat ttaagagctt attacgagtt acctcacata atttagaatc aatttctatc
      721 gccgttacaa aattacatct ctttaccaat ccagcagtaa aatgaccttt ccctgcacct
      781 atttcaaaga tgttatcttt ttcatctaaa cttatgcaat tcattatttt ttctatgtga
      841 tattttgaag taataaaatt ttgactatct tttatattta ctttgttcat tataacctct
      901 ccttaattta ttgcatctct tttcgaatat ttatgttttt tgagaaaaga acgtactcat
      961 ggttcatccc gatatgcgta tcggtctgta tatcagcaac tttctatgtg tttcaactac
     1021 aatagtcatc tattctcatc tttctgagtc caccccctgc aaagcccctc tttacgacat
     1081 aaaaattcgg tcggaaaagg tatgcaaaag atgtttctct ctttaagaga aactcttcgg
     1141 gatgcaaaaa tatgaaaata actccaattc accaaattat atagcgactt ttttacaaaa
     1201 tgctaaaatt tgttgatttc cgtcaagcaa ttgttgagca aaaatgtctt ttacgataaa
     1261 atgatacctc aatatcaact gtttagcaaa acgatatttc tcttaaagag agaaacacct
     1321 ttttgttcac caatccccga cttttaatcc cgcggccatg attgaaaaag gaagagtatg
     1381 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1441 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     1501 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     1561 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     1621 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     1681 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     1741 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     1801 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     1861 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     1921 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     1981 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2041 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtcacgc
     2101 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2161 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     2221 ctgattaagc attggtaact gtcagaccaa gtttactcat aacgcgtcaa ttcgaggggg
     2281 atcaattccg tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc
     2341 ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg
     2401 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct
     2461 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
     2521 ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata
     2581 atgaccccga agcagggtta tgcagcggaa aacggaattg atccggccac gatgcgtccg
     2641 gcgtagagga tctgaagatc agcagttcaa cctgttgata gtacgtacta agctctcatg
     2701 tttcacgtac taagctctca tgtttaacgt actaagctct catgtttaac gaactaaacc
     2761 ctcatggcta acgtactaag ctctcatggc taacgtacta agctctcatg tttcacgtac
     2821 taagctctca tgtttgaaca ataaaattaa tataaatcag caacttaaat agcctctaag
     2881 gttttaagtt ttataagaaa aaaaagaata tataaggctt ttaaagcttt taaggtttaa
     2941 cggttgtgga caacaagcca gggatgtaac gcactgagaa gcccttagag cctctcaaag
     3001 caattttgag tgacacagga acacttaacg gctgacatgg gaattcccct ccaccgcggt
     3061 ggattaatgc ggctgcaatt tttttgggcg gggccgccca aaaaaatcct agcaccctgc
     3121 agggtctccc gctttacaaa gaaaattcga caaactgtta tttttctatc tatttaaatg
     3181 tacacccgtt tgtttacttt actttagtta tgtacctttg tcggcaatgt acacccgaaa
     3241 gtttactttc aaagataata gaacttaagg ttttagagct agaaatagca agttaaaata
     3301 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttggatccaa
     3361 aaaaaaaaaa caccctaacg ggtgtttttt tttttttggt ctgccgcggc cgctgctgtc
     3421 ataggccggt tgtccggtag tccggatgat ttaaggaaga tttctcttct tttttcactt
     3481 tctccgaaca acctccactt aagttctatt atctttgcag tagctgtcac cggatgtgct
     3541 ttccggtctg atgagtccgt gaggacgaaa cagcctctac aaataatttt gtttaacgca
     3601 ttttaaaata aaataaatta tttatttaat taaacgaata tggtttttac tctggaagat
     3661 tttgttggcg attggcgtca gaccgcgggt tataatttgg atcaagtcct ggaacagggt
     3721 ggcgtaagct ctctgttcca gaacctgggt gtgagcgtga cgccgattca gcgcatcgtt
     3781 ctgtccggcg agaacggtct gaaaattgat attcatgtga tcatcccgta cgaaggcctg
     3841 agcggtgacc aaatgggtca aatcgagaaa atctttaaag tcgtctaccc agttgacgat
     3901 caccacttca aggttatctt gcattacggt acgctggtga ttgatggtgt gaccccgaat
     3961 atgattgact atttcggccg tccgtatgaa ggcattgccg tttttgacgg taaaaagatc
     4021 accgtcaccg gtaccctgtg gaatggcaat aagattattg acgagcgtct gattaacccg
     4081 gacggcagcc tgctgttccg cgtgaccatc aacggtgtca cgggttggcg tctgtgcgag
     4141 cgcatcctgg cataatgaac tgggatccct cggtaccaaa ttttcgaaaa aagacgctga
     4201 aaagcgtctt ttttcgtttt ggtccgcggc cgccggccgc tgatctggaa gaagcaatga
     4261 aagctgctgt taagtctccg aatcaggtat tgttcctgac aggtgtattc ccatccggta
     4321 aacgcggata ctttgcagtt gatctgactc aggaataaat tataaattaa ggtaagaaga
     4381 ttgtaggata agctaatgaa atagaaaaag gatgccgtca cacaacttgt cggcattctt
     4441 ttttgtttta ttagttgaaa atatagtgaa aaagttgcct aaatatgtat gttaacaaat
     4501 tatttgtcgt aactttgcac tccaaatctg tttttaacat atggcactaa tgcagaaaaa
     4561 attgactcgt tctcagcaga aacacttgga catcatcaac gctgctaaag aagaattcat
     4621 cgaattcggt ttcttggctg ctaacatgga ccgtatcact tcttctgctg aagtatctaa
     4681 acgtactttg taccgtcact tcgaatctaa agaagtattg ttcgaatctg tattgactat
     4741 catcaacgac tctgtaaacg aatctatctc ttaccacttc gacccgaaca aatctactga
     4801 agaacagttg actgaaatcg cttacaaaga aatcgacgta ttgtacaaaa cttacggtat
     4861 cgctttggct cgtactatcg taatggaatt cttgcgtcag ccggaaatgg ctaaaacttt
     4921 gatccagaac atctactcta tccgtgctat cactcagtgg ttccgttctg ctatcgaagc
     4981 taaacgtttg aaagacgctg acccgaaatt gatgactgac gtatacgtat ctttgttcca
     5041 gggtttgttc ttctggccgc aggtaatgca cttggacttg gaaccgcacg gtgaagaatt
     5101 gtctcagaaa atcgaaactt tgactactat cttcttgcag tcttacggtg tagctgaata
     5161 acaatgaatg ggctttcctc ggtaccaaat tccagaaaag aggcctcccg aaaggggggc
     5221 cttttttcgt tttggtccct aattgcctat cttccagtga tggaacagca tttgtgcatt
     5281 ggctgcaaca atcagcctta cttgtgcctg ttctatttcc gaaccgaccg cttgtatgaa
     5341 tccatcaaaa ttcgttttct ctatgttgga ttccttgttg ctcatattgt gatgataatt
     5401 tctacaaata tagtcattgg taactatcta tgaaactgtt tgatactttt actcatcgga
     5461 ttcttttaat atcgctactc tctacgttca accaaaagaa ataatgactt actgctatat
     5521 tttttgcacg tgtggggaaa atgtggggaa aattcaagca aaagaaaaag ctaagtattg
     5581 aactatcaaa tacttagctt tctttcttgt acccagaccc cgcatttgaa ataattaaag
     5641 tggggaaaat gtgggtaaaa agaaaaatgc ggaaaaacgc cacaattaca ctgtatttca
     5701 atatgttata atcctattaa attttaatcc aagtttaatc gaattgcaaa atatttagca
     5761 gatgtgggga aaatgctggg gaaaatattt atatttgcag cagagtaaaa ttcaacctga
     5821 aagtctatga aagtaacctt tatcattaaa aaagcagcca aacgatatga tacagaatcc
     5881 atggctacaa tctatgtccg ttttagaaac ggaaggcagt tagactccgt tgctcctact
     5941 cagttagcca tcaatcccaa tctatgggat gataaagacg aatgtgtaaa aacgaaagct
     6001 gtctgcaatg aagaaatgcg tacccatata aatgaagaga tacgccagtt gaaaacctat
     6061 atcgagaagg tatatcaaca agaaaaggaa gcaatagaca aagaatggct aaaaacaaca
     6121 cttgataaat tttaccatcc tgaaaaatat tttttgccgg aggaagtggt tatcaagcct
     6181 accattggag aactattcga tgaatttcta aacaagcacc ctttgtcgga agtacgaaag
     6241 aaaaatttcc gggttgtcaa aagagcctta ctgcgttatg aactatatgt aagggctaca
     6301 aagagaggac aaaagggctt tatccttgat gtggatttgg taacacctga cacgcttcgg
     6361 gatatgtggg atttctttca gaacgaatac cagtattatg aactttaccc gagcatttat
     6421 gaagccattc ccgaaaagag gacaccacag cccagaagca aaaacacgct gatagactgt
     6481 ttttcaagaa tacgcacatt cttcctgtgg tgcttcgata acaaacgcac cacaaacaga
     6541 cctttcgaca agtttccgat agaggagtgt acatatggta caccttatta tataacactc
     6601 gaagaaaggg acaggatttt taatgcagac ctttctgcca ccccacaact ggcaatacag
     6661 agggatatat tcatatttca gacactgata ggatgcaggg tgagcgacct gtaccgaatg
     6721 accaaactaa atgtggtcaa tgaagccata gaatatattc ccaagaaaac caaagagggg
     6781 aatccggtta cggtacgtgt tccacttaac gacaaagcga aagaaatcct tgaacgctac
     6841 aaagaatatg agggaaaact gttgccgttc atatccgagc aaaagtacaa tgatgccata
     6901 aaaaagatat tcaaattagc tggagttgac cgcatcgtaa caatcttaga cccgttgacg
     6961 cacaacgaaa tcaaacgacc tatttatgaa gtggcaagca gccatctggc aagacgtacg
     7021 tttatcggca atatctataa aaaagtgaaa gacccgaacc ttgtttccgc actgtcggga
     7081 cacaaggagg gaagcaaagc tttcagacga tacagggata ttgacgaaga aatgaagaaa
     7141 gaccttgtaa aactactgga ctgataaaag tatcaaacag tttcatagat agtt
//
