LOCUS       Exported                7237 bp ds-DNA     circular SYN 25-JUN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7237)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 20, 2020 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7237
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..30
                     /label=mmD661
     misc_feature    1
                     /label=origin
     misc_recomb     118..2262
                     /label=NluI fragment from pKNOCK
                     /note="NluI fragment from pKNOCK"
     CDS             complement(156..890)
                     /label=ermG
                     /note="ermG"
     CDS             1378..2239
                     /label=beta-lactamase from pBR322
                     /note="beta-lactamase from pBR322"
     primer_bind     1837..1856
                     /label=oMT203
     primer_bind     complement(2089..2106)
                     /label=oMT1189
     primer_bind     2089..2106
                     /label=oMT1184
     misc_feature    2097
                     /label=t2096a
     misc_recomb     2097
                     /label=NluI fragment from pKNOCK
                     /note="NluI fragment from pKNOCK"
     CDS             2097
                     /label=beta-lactamase from pBR322
                     /note="beta-lactamase from pBR322"
     misc_recomb     2277..3062
                     /label=RP4 oriT/RSK oriV
                     /note="RP4 oriT/RSK oriV"
     primer_bind     complement(2340..2359)
                     /label=oMT204
     primer_bind     2968..2987
                     /label=oMT25
     primer_bind     2968..2987
                     /label=pNBU1_seq.FOR
     primer_bind     complement(3040..3057)
                     /label=oMT1267
     misc_feature    3062
                     /label=3062 mutation from ""g"" to ""t""
                     /note="Mutated in pMT1fix2, but will not fix, since it's at
                     the backbone of the vector and is not CDS or a region with 
                     important UTR."
     misc_feature    3063..3122
                     /label=pNBU2-Lac-dCas9-NL3 PCR Product - pNBU2-Lac-dCas9-
                     /note="Geneious type: Inserted sequence"
     terminator      3078..3107
                     /label=tL17 terminator
                     /label=tL17
     primer_bind     complement(3099..3122)
                     /label=oMT912
     misc_feature    3103..3122
                     /label=Feature 84
     primer_bind     3123..3159
                     /label=oMT1293
     misc_feature    3130..3133
                     /label=CGCT
     promoter        3134..3176
                     /label=cfxA_promoter
     misc_feature    3177..3185
                     /label=AANGTANAC
     misc_feature    3186..3191
                     /label=N6
     misc_feature    3189..3192
                     /label=-33
     misc_feature    3192..3200
                     /label=GTNTACNTT
     -35_signal      3193..3198
                     /label=-35
     promoter        3201..3212
                     /label=PcfxA
     misc_feature    3213..3220
                     /label=-7
     misc_feature    3222..3225
                     /label=4bp-2
     primer_bind     complement(3223..3249)
                     /label=oMT916
     misc_feature    3225
                     /label=+1
     misc_feature    3226..3234
                     /label=AANGTANAC
     misc_feature    3235..3240
                     /label=N6
     misc_feature    3241..3249
                     /label=GTNTACNTT
     -35_signal      3242..3247
                     /label=-35
     misc_feature    3250..3269
                     /label=sgRNA-8
     misc_feature    3270..3311
                     /label=chimera tracrRNA
     primer_bind     3270..3303
                     /label=oMT915
     misc_feature    3316..3352
                     /label=S. pyogenes terminator
     primer_bind     complement(3333..3473)
                     /label=oMT1277
     misc_feature    3353..3448
                     /label=ECK120029600
     misc_feature    3354..3380
                     /label=Feature 73
     misc_feature    3354..3358
                     /label=Amp up this region
     misc_feature    3364
                     /label=C to T mutation
     misc_feature    3449..3456
                     /label=ECK120029600
     misc_feature    3449..3450
                     /label=Restriction Sites
     misc_feature    3453..3456
                     /label=Feature 72
     primer_bind     3456..3582
                     /label=oMT914
     misc_feature    3457..3540
                     /label=PBF2884
     primer_bind     3457..3476
                     /label=oMT1006
     misc_feature    3499..3503
                     /label=-60
     primer_bind     complement(3507..3535)
                     /label=oMT1116
     misc_feature    3510..3514
                     /label=-50
     primer_bind     3516..3540
                     /label=oMT1114
     primer_bind     complement(3517..3540)
                     /label=oMT1064
     misc_feature    3529..3532
                     /label=-33
     misc_feature    3533..3540
                     /label=20bp
     misc_feature    3538..3539
                     /label=PAM
     misc_feature    3541..3560
                     /label=sgRNA-8
     misc_feature    3565..3639
                     /label=RiboJ00
     terminator      3640..3682
                     /label=rpiL*
                     /note="rpiL* termninator"
     CDS             3683..4198
                     /codon_start=1
                     /label=Luciferase Reporter
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     3683..3707
                     /label=oMT41
     primer_bind     complement(3694..3713)
                     /label=oMT386
     primer_bind     3971..3990
                     /label=oMT399
     primer_bind     complement(4177..4202)
                     /label=oMT1343
     misc_feature    4199..5204
                     /label=Amp up this region
     primer_bind     4199..4309
                     /label=oMT1344
     misc_feature    4199..4283
                     /label=Amp up this region
     misc_feature    4199..4202
                     /label=TGAA
     misc_feature    4206..4276
                     /label=L3S2P56
     misc_feature    4269..4270
                     /label=Restriction Sites
     promoter        4284..4566
                     /label=BT1311_promoter
     primer_bind     4284..4307
                     /label=oMT1308
     primer_bind     4462..4481
                     /label=oMT1135
     -35_signal      4527..4530
                     /label=-33
     primer_bind     4548..4567
                     /label=oMT100
     -10_signal      4553..4560
                     /label=-7
     RBS             4567..4592
                     /label=BT1311_RBS
     CDS             4593..5204
                     /codon_start=1
                     /label=VFA0359-Bt
                     /translation="MQKKLTRSQQKHLDIINAAKEEFIEFGFLAANMDRITSSAEVSKR
                     TLYRHFESKEVLFESVLTIINDSVNESISYHFDPNKSTEEQLTEIAYKEIDVLYKTYGI
                     ALARTIVMEFLRQPEMAKTLIQNIYSIRAITQWFRSAIEAKRLKDADPKLMTDVYVSLF
                     QGLFFWPQVMHLDLEPHGEELSQKIETLTTIFLQSYGVAE"
     CDS             4593..4595
                     /codon_start=1
                     /label=CmeR(NCTC11168)
                     /translation="M"
     gene            4593..4595
                     /gene="rpoD"
                     /gene_synonym="alt; ECK3057; JW3039"
                     /locus_tag="b3067"
                     /label=rpoD
                     /db_xref="EcoGene:EG10896"
                     /db_xref="GeneID:947567"
     CDS             4593..4595
                     /codon_start=1
                     /label=BreR-Bt
                     /translation="M"
     primer_bind     4862..4883
                     /label=internal seq primer
     primer_bind     4862..4883
                     /label=oMT379
     primer_bind     complement(5175..5199)
                     /label=oMT677
     primer_bind     complement(5182..5209)
                     /label=oMT1309
     CDS             5202..5204
                     /codon_start=1
                     /label=CmeR(NCTC11168)
                     /translation=""
     gene            5202..5204
                     /gene="rpoD"
                     /gene_synonym="alt; ECK3057; JW3039"
                     /locus_tag="b3067"
                     /label=rpoD
                     /db_xref="EcoGene:EG10896"
                     /db_xref="GeneID:947567"
     CDS             5202..5204
                     /codon_start=1
                     /label=BreR-Bt
                     /translation=""
     CDS             5202..5204
                     /label=CmeR(NCTC11168)
     gene            5202..5204
                     /label=rpoD
     CDS             5202..5204
                     /label=BreR-Bt
     misc_feature    5205..5214
                     /label=Amp up this region
     misc_feature    5205..5208
                     /label=CAAT
     primer_bind     5215..5229
                     /label=oMT676
     misc_feature    5215..5220
                     /label=problematic assembly region
     misc_feature    5215..5220
                     /label=Feature 69
     terminator      5221..5281
                     /label=L3S2P21 Terminator
     primer_bind     complement(5260..5300)
                     /label=oMT1272
     primer_bind     5282..5309
                     /label=oMT1259
     primer_bind     complement(5407..5427)
                     /label=pNBU1_seq.REV
     misc_feature    complement(5456..5494)
                     /label=mmD660
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    5495..7237
                     /label=intN1 Construct PCR Product - intN1 Construct P
                     /label=intN1 Construct PCR Product - intN1 Construct PCR 
                     Product 2
                     /note="Geneious type: Inserted sequence"
     misc_feature    5495..6294
                     /label=Piece1
     misc_feature    5495..5524
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
     primer_bind     5495..5523
                     /label=mmD658
     primer_bind     5516..5536
                     /label=oMT113
     misc_feature    5525..5854
                     /label=attN1 Region
     primer_bind     5525..5544
                     /label=intN1 Construct PCR Product (fwd) - pNBU2-PcfxA
                     /label=intN1 Construct PCR Product (fwd) - 
                     pNBU2-PcfxA-NanoLuc PCR Product
     misc_feature    5575..5590
                     /label=DR1
     misc_feature    5608..5622
                     /label=IR1
     misc_feature    5632..5646
                     /label=IR2
     misc_feature    5652..5665
                     /label=CC Region
     misc_feature    5664
                     /label=G-->C
     misc_feature    5664
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     misc_feature    5665
                     /label='-1'
     misc_feature    5666
                     /label='+1'
     misc_feature    5683..5698
                     /label=DR2
     primer_bind     complement(5721..5743)
                     /label=oMT114
     misc_feature    5807..5823
                     /label=DR3
     CDS             5870..6294
                     /codon_start=1
                     /transl_table=11
                     /gene="intN1"
                     /product="IntN1"
                     /label=intN1
                     /label=intN1 CDS
                     /note="NBU1 integrase"
                     /db_xref="GI:8308020"
                     /protein_id="AAF74437.1"
                     /translation="MKVTFIIKKAAKRYDTESMATIYVRFRNGRQLDSVAPTQLAINPN
                     LWDDKDECVKTKAVCNEEMRTHINEEIRQLKTYIEKVYQQEKEAIDKEWLKTTLDKFYH
                     PEKYFLPEEVVIKPTIGELFDEFLNKHPLSEVRKKNF"
     gene            5870..6294
                     /gene="intN1"
                     /label=intN1
                     /label=intN1 gene
     primer_bind     complement(6086..6113)
                     /label=oMT157
     primer_bind     6086..6113
                     /label=oMT156
     misc_feature    complement(6264..6294)
                     /label=mmD659
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    6265..7237
                     /label=Piece2
     CDS             6265..7207
                     /codon_start=1
                     /transl_table=11
                     /gene="intN1"
                     /product="IntN1"
                     /label=intN1
                     /label=intN1 CDS
                     /note="NBU1 integrase"
                     /db_xref="GI:8308020"
                     /protein_id="AAF74437.1"
                     /translation="MVGSTKEKFPGCQKSLTAL*TICKGYKERTKGLYP*CGFGNT*HA
                     SGYVGFLSERIPVL*TLPEHL*SHSRKEDTTAQKQKHADRLFFKNTHILPVVLR*QTHH
                     KQTFRQVSDRGVYIWYTLLYNTRRKGQDF*CRPFCHPTTGNTEGYIHISDTDRMQGERP
                     VPNDQTKCGQ*SHRIYSQENQRGESGYGTCST*RQSERNP*TLQRI*GKTVAVHIRAKV
                     Q*CHKKDIQISWS*PHRNNLRPVDAQRNQTTYL*SGKQPSGKTYVYRQYL*KSERPEPC
                     FRTVGTQGGKQSFQTIQGY*RRNEERPCKTTGL"
     gene            6265..7207
                     /gene="intN1"
                     /label=intN1
                     /label=intN1 gene
     misc_feature    6265..6294
                     /label=Piece2
     misc_feature    6265..6294
                     /label=Ligation
     primer_bind     6265..6284
                     /label=intN1 Construct PCR Product 2 (fwd)
     misc_feature    complement(6270..6294)
                     /label=intN1 Construct PCR Product (rev)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(7183..7209)
                     /label=intN1 Construct PCR Product 2 (rev) - pNBU2-Pcf
                     /label=intN1 Construct PCR Product 2 (rev) - 
                     pNBU2-PcfxA-NanoLuc PCR Product
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    7199
                     /label=T
                     /note="Geneious type: Editing History Replacement"
     misc_feature    complement(7201..7237)
                     /label=mmD661
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    7201
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    7208..7237
                     /label=HOMOLOGY
     misc_feature    7210..7237
                     /label=CTTGTAGTTCGTTTAATTCGCTTTTCGTTTTTACGAATTTAACGAAT
                     /label=CTTGTAGTTCGTTTAATTCGCTTTTCGTTTTTACGAATTTAACGAATTGA 
                     ATATCATTGTTTTACGATTGATTTTCATTATATATTTGCGCCACTATCCTAATCGGGG 
                     TAGTGGCTTTTCGTTTATAATACTGCCGAAACGGGAA
                     /note="Geneious type: Editing History Replacement"
ORIGIN
        1 agagtagcga tattaaaaga atccgatgag aaaagactaa tatttatcta tccattcagt
       61 ttgatttttc aggactttac atcgtcctga aagtatttgt tggtaccggt accgaggacg
      121 cgtaaacatt tacagttgca tgtggcctat tgtttttagc cgttaaatat tttataacta
      181 ttaaatagcg atacaaattg ttcgaaacta atattgttta tatcatatat tctcgcatgt
      241 tttaaagctt tattaaattg attttttgta aacagttttt cgtactcttt gttaacccat
      301 ttcattacaa aagtttcata tttttttctc tctttaaatg ccatttttgc tggctttctt
      361 tttaatacaa ttaatgtgct atccacttta ggttttggat ggaaataata cctaggaatt
      421 tttgctaata tagaaatatc tacctctgcc attaacagca atgctagtga tctgtttgta
      481 tctaataaca ttttagcaaa accatattcc actattaaat aacttattgt ggctgaactt
      541 tcaaaaacaa tttttcgaat tatatttgtg cttatgttgt aaggtatgct gccaaatatt
      601 ttatatggat tgtggctagg aaatgtaaat ttcagtatat catcatttac tatttgatag
      661 ttaggataat ttaagagctt attacgagtt acctcacata atttagaatc aatttctatc
      721 gccgttacaa aattacatct ctttaccaat ccagcagtaa aatgaccttt ccctgcacct
      781 atttcaaaga tgttatcttt ttcatctaaa cttatgcaat tcattatttt ttctatgtga
      841 tattttgaag taataaaatt ttgactatct tttatattta ctttgttcat tataacctct
      901 ccttaattta ttgcatctct tttcgaatat ttatgttttt tgagaaaaga acgtactcat
      961 ggttcatccc gatatgcgta tcggtctgta tatcagcaac tttctatgtg tttcaactac
     1021 aatagtcatc tattctcatc tttctgagtc caccccctgc aaagcccctc tttacgacat
     1081 aaaaattcgg tcggaaaagg tatgcaaaag atgtttctct ctttaagaga aactcttcgg
     1141 gatgcaaaaa tatgaaaata actccaattc accaaattat atagcgactt ttttacaaaa
     1201 tgctaaaatt tgttgatttc cgtcaagcaa ttgttgagca aaaatgtctt ttacgataaa
     1261 atgatacctc aatatcaact gtttagcaaa acgatatttc tcttaaagag agaaacacct
     1321 ttttgttcac caatccccga cttttaatcc cgcggccatg attgaaaaag gaagagtatg
     1381 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1441 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     1501 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     1561 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     1621 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     1681 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     1741 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     1801 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     1861 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     1921 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     1981 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2041 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtcacgc
     2101 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2161 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     2221 ctgattaagc attggtaact gtcagaccaa gtttactcat aacgcgtcaa ttcgaggggg
     2281 atcaattccg tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc
     2341 ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg
     2401 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct
     2461 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
     2521 ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata
     2581 atgaccccga agcagggtta tgcagcggaa aacggaattg atccggccac gatgcgtccg
     2641 gcgtagagga tctgaagatc agcagttcaa cctgttgata gtacgtacta agctctcatg
     2701 tttcacgtac taagctctca tgtttaacgt actaagctct catgtttaac gaactaaacc
     2761 ctcatggcta acgtactaag ctctcatggc taacgtacta agctctcatg tttcacgtac
     2821 taagctctca tgtttgaaca ataaaattaa tataaatcag caacttaaat agcctctaag
     2881 gttttaagtt ttataagaaa aaaaagaata tataaggctt ttaaagcttt taaggtttaa
     2941 cggttgtgga caacaagcca gggatgtaac gcactgagaa gcccttagag cctctcaaag
     3001 caattttgag tgacacagga acacttaacg gctgacatgg gaattcccct ccaccgcggt
     3061 ggattaatgc ggctgcaatt tttttgggcg gggccgccca aaaaaatcct agcaccctgc
     3121 agggtctccc gctttacaaa gaaaattcga caaactgtta tttttctatc tatttaaatg
     3181 tacacccgtt tgtttacttt actttagtta tgtacctttg tcggcaatgt acacccgaaa
     3241 gtttactttc aaagataaat cagagaaaag ttttagagct agaaatagca agttaaaata
     3301 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttggatcctt
     3361 cagccaaaaa acttaagacc gccggtcttg tccactacct tgcagtaatg cggtggacag
     3421 gatcggcggt tttcttttct cttctcaagc ggccgctgct gtcataggcc ggttgtccgg
     3481 tagtccggat gatttaagga agatttctct tcttttttca ctttctccga acaacctcca
     3541 ttttctctga tttatctttg cagtagctgt caccggatgt gctttccggt ctgatgagtc
     3601 cgtgaggacg aaacagcctc tacaaataat tttgtttaac gcattttaaa ataaaataaa
     3661 ttatttattt aattaaacga atatggtttt tactctggaa gattttgttg gcgattggcg
     3721 tcagaccgcg ggttataatt tggatcaagt cctggaacag ggtggcgtaa gctctctgtt
     3781 ccagaacctg ggtgtgagcg tgacgccgat tcagcgcatc gttctgtccg gcgagaacgg
     3841 tctgaaaatt gatattcatg tgatcatccc gtacgaaggc ctgagcggtg accaaatggg
     3901 tcaaatcgag aaaatcttta aagtcgtcta cccagttgac gatcaccact tcaaggttat
     3961 cttgcattac ggtacgctgg tgattgatgg tgtgaccccg aatatgattg actatttcgg
     4021 ccgtccgtat gaaggcattg ccgtttttga cggtaaaaag atcaccgtca ccggtaccct
     4081 gtggaatggc aataagatta ttgacgagcg tctgattaac ccggacggca gcctgctgtt
     4141 ccgcgtgacc atcaacggtg tcacgggttg gcgtctgtgc gagcgcatcc tggcataatg
     4201 aactgggatc cctcggtacc aaattttcga aaaaagacgc tgaaaagcgt cttttttcgt
     4261 tttggtccgc ggccgccggc cgctgatctg gaagaagcaa tgaaagctgc tgttaagtct
     4321 ccgaatcagg tattgttcct gacaggtgta ttcccatccg gtaaacgcgg atactttgca
     4381 gttgatctga ctcaggaata aattataaat taaggtaaga agattgtagg ataagctaat
     4441 gaaatagaaa aaggatgccg tcacacaact tgtcggcatt cttttttgtt ttattagttg
     4501 aaaatatagt gaaaaagttg cctaaatatg tatgttaaca aattatttgt cgtaactttg
     4561 cactccaaat ctgtttttaa catatggcac taatgcagaa aaaattgact cgttctcagc
     4621 agaaacactt ggacatcatc aacgctgcta aagaagaatt catcgaattc ggtttcttgg
     4681 ctgctaacat ggaccgtatc acttcttctg ctgaagtatc taaacgtact ttgtaccgtc
     4741 acttcgaatc taaagaagta ttgttcgaat ctgtattgac tatcatcaac gactctgtaa
     4801 acgaatctat ctcttaccac ttcgacccga acaaatctac tgaagaacag ttgactgaaa
     4861 tcgcttacaa agaaatcgac gtattgtaca aaacttacgg tatcgctttg gctcgtacta
     4921 tcgtaatgga attcttgcgt cagccggaaa tggctaaaac tttgatccag aacatctact
     4981 ctatccgtgc tatcactcag tggttccgtt ctgctatcga agctaaacgt ttgaaagacg
     5041 ctgacccgaa attgatgact gacgtatacg tatctttgtt ccagggtttg ttcttctggc
     5101 cgcaggtaat gcacttggac ttggaaccgc acggtgaaga attgtctcag aaaatcgaaa
     5161 ctttgactac tatcttcttg cagtcttacg gtgtagctga ataacaatga atgggctttc
     5221 ctcggtacca aattccagaa aagaggcctc ccgaaagggg ggcctttttt cgttttggtc
     5281 cctaattgcc tatcttccag tgatggaaca gcatttgtgc attggctgca acaatcagcc
     5341 ttacttgtgc ctgttctatt tccgaaccga ccgcttgtat gaatccatca aaattcgttt
     5401 tctctatgtt ggattccttg ttgctcatat tgtgatgata atttctacaa atatagtcat
     5461 tggtaactat ctatgaaact gtttgatact tttactcatc ggattctttt aatatcgcta
     5521 ctctctacgt tcaaccaaaa gaaataatga cttactgcta tattttttgc acgtgtgggg
     5581 aaaatgtggg gaaaattcaa gcaaaagaaa aagctaagta ttgaactatc aaatacttag
     5641 ctttctttct tgtacccaga ccccgcattt gaaataatta aagtggggaa aatgtgggta
     5701 aaaagaaaaa tgcggaaaaa cgccacaatt acactgtatt tcaatatgtt ataatcctat
     5761 taaattttaa tccaagttta atcgaattgc aaaatattta gcagatgtgg ggaaaatgct
     5821 ggggaaaata tttatatttg cagcagagta aaattcaacc tgaaagtcta tgaaagtaac
     5881 ctttatcatt aaaaaagcag ccaaacgata tgatacagaa tccatggcta caatctatgt
     5941 ccgttttaga aacggaaggc agttagactc cgttgctcct actcagttag ccatcaatcc
     6001 caatctatgg gatgataaag acgaatgtgt aaaaacgaaa gctgtctgca atgaagaaat
     6061 gcgtacccat ataaatgaag agatacgcca gttgaaaacc tatatcgaga aggtatatca
     6121 acaagaaaag gaagcaatag acaaagaatg gctaaaaaca acacttgata aattttacca
     6181 tcctgaaaaa tattttttgc cggaggaagt ggttatcaag cctaccattg gagaactatt
     6241 cgatgaattt ctaaacaagc accctttgtc ggaagtacga aagaaaaatt tccgggttgt
     6301 caaaagagcc ttactgcgtt atgaactata tgtaagggct acaaagagag gacaaaaggg
     6361 ctttatcctt gatgtggatt tggtaacacc tgacacgctt cgggatatgt gggatttctt
     6421 tcagaacgaa taccagtatt atgaacttta cccgagcatt tatgaagcca ttcccgaaaa
     6481 gaggacacca cagcccagaa gcaaaaacac gctgatagac tgtttttcaa gaatacgcac
     6541 attcttcctg tggtgcttcg ataacaaacg caccacaaac agacctttcg acaagtttcc
     6601 gatagaggag tgtacatatg gtacacctta ttatataaca ctcgaagaaa gggacaggat
     6661 ttttaatgca gacctttctg ccaccccaca actggcaata cagagggata tattcatatt
     6721 tcagacactg ataggatgca gggtgagcga cctgtaccga atgaccaaac taaatgtggt
     6781 caatgaagcc atagaatata ttcccaagaa aaccaaagag gggaatccgg ttacggtacg
     6841 tgttccactt aacgacaaag cgaaagaaat ccttgaacgc tacaaagaat atgagggaaa
     6901 actgttgccg ttcatatccg agcaaaagta caatgatgcc ataaaaaaga tattcaaatt
     6961 agctggagtt gaccgcatcg taacaatctt agacccgttg acgcacaacg aaatcaaacg
     7021 acctatttat gaagtggcaa gcagccatct ggcaagacgt acgtttatcg gcaatatcta
     7081 taaaaaagtg aaagacccga accttgtttc cgcactgtcg ggacacaagg agggaagcaa
     7141 agctttcaga cgatacaggg atattgacga agaaatgaag aaagaccttg taaaactact
     7201 ggactgataa aagtatcaaa cagtttcata gatagtt
//
