LOCUS       Exported                7204 bp ds-DNA     circular SYN 20-JUL-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 20, 2020 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..30
                     /label=mmD661
     misc_feature    1
                     /label=origin
     misc_recomb     118..2262
                     /label=NluI fragment from pKNOCK
                     /note="NluI fragment from pKNOCK"
     CDS             complement(156..890)
                     /label=ermG
                     /note="ermG"
     CDS             1378..2239
                     /label=beta-lactamase from pBR322
                     /note="beta-lactamase from pBR322"
     primer_bind     2089..2106
                     /label=oMT1184
     primer_bind     complement(2089..2106)
                     /label=oMT1189
     CDS             2097
                     /label=beta-lactamase from pBR322
                     /note="beta-lactamase from pBR322"
     misc_recomb     2097
                     /label=NluI fragment from pKNOCK
                     /note="NluI fragment from pKNOCK"
     misc_feature    2097
                     /label=t2096a
     misc_recomb     2277..3062
                     /label=RP4 oriT/RSK oriV
                     /note="RP4 oriT/RSK oriV"
     primer_bind     2968..2987
                     /label=oMT25
     primer_bind     2968..2987
                     /label=pNBU1_seq.FOR
     primer_bind     complement(3040..3057)
                     /label=oMT1267
     misc_feature    3062
                     /label=3062 mutation from ""g"" to ""t""
                     /note="Mutated in pMT1fix2, but will not fix, since it's at
                     the backbone of the vector and is not CDS or a region with 
                     important UTR."
     misc_feature    3063..3122
                     /label=pNBU2-Lac-dCas9-NL3 PCR Product - pNBU2-Lac-dCas9-
                     /note="Geneious type: Inserted sequence"
     terminator      3078..3107
                     /label=tL17 terminator
                     /label=tL17
     primer_bind     complement(3099..3122)
                     /label=oMT912
     misc_feature    3103..3122
                     /label=Feature 84
     primer_bind     3123..3159
                     /label=oMT1293
     misc_feature    3130..3133
                     /label=CGCT
     promoter        3134..3176
                     /label=cfxA_promoter
     misc_feature    3177..3185
                     /label=AANGTANAC
     misc_feature    3186..3191
                     /label=N6
     misc_feature    3189..3192
                     /label=-33
     misc_feature    3192..3200
                     /label=GTNTACNTT
     -35_signal      3193..3198
                     /label=-35
     promoter        3201..3212
                     /label=PcfxA
     misc_feature    3213..3220
                     /label=-7
     misc_feature    3222..3225
                     /label=4bp-2
     primer_bind     complement(3223..3249)
                     /label=oMT916
     misc_feature    3225
                     /label=+1
     misc_feature    3226..3234
                     /label=AANGTANAC
     misc_feature    3235..3240
                     /label=N6
     misc_feature    3241..3249
                     /label=GTNTACNTT
     -35_signal      3242..3247
                     /label=-35
     misc_feature    3250..3269
                     /label=sgRNA-9
     primer_bind     3269..3303
                     /label=oMT915
     misc_feature    3270..3311
                     /label=chimera tracrRNA
     misc_feature    3316..3352
                     /label=S. pyogenes terminator
     primer_bind     complement(3333..3440)
                     /label=oMT1313
     primer_bind     complement(3333..3440)
                     /label=oMT1278
     misc_feature    3353..3358
                     /label=Amp up this region
     misc_feature    3359..3415
                     /label=ECK120034435
     misc_feature    3416..3423
                     /label=Restriction Sites
     misc_feature    3420..3421
                     /label=mutated from CC to TG
     primer_bind     3423..3549
                     /label=oMT914
     misc_feature    3424..3507
                     /label=PBF2884
     primer_bind     3424..3443
                     /label=oMT1006
     misc_feature    3424..3440
                     /label=Feature 88
     misc_feature    3466..3470
                     /label=-60
     primer_bind     complement(3474..3502)
                     /label=oMT1116
     misc_feature    3477..3481
                     /label=-50
     primer_bind     3483..3507
                     /label=oMT1114
     primer_bind     complement(3484..3507)
                     /label=oMT1064
     misc_feature    3496..3499
                     /label=-33
     misc_feature    3500..3507
                     /label=20bp
     misc_feature    3505..3506
                     /label=PAM
     misc_feature    3508..3527
                     /label=sgRNA-9
     misc_feature    3532..3606
                     /label=RiboJ00
     terminator      3607..3649
                     /label=rpiL*
                     /note="rpiL* termninator"
     CDS             3650..4165
                     /codon_start=1
                     /label=Luciferase Reporter
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     3650..3674
                     /label=oMT41
     primer_bind     complement(3661..3680)
                     /label=oMT386
     primer_bind     3938..3957
                     /label=oMT399
     misc_feature    4137..5187
                     /label=problematic assembly region
     primer_bind     complement(4144..4169)
                     /label=oMT1343
     primer_bind     complement(4146..4165)
                     /label=oMT1295
     misc_feature    4166..5171
                     /label=Amp up this region
     primer_bind     4166..4276
                     /label=oMT1344
     misc_feature    4166..4250
                     /label=Amp up this region
     misc_feature    4166..4169
                     /label=TGAA
     misc_feature    4173..4243
                     /label=L3S2P56
     misc_feature    4236..4237
                     /label=Restriction Sites
     promoter        4251..4533
                     /label=BT1311_promoter
     primer_bind     4251..4274
                     /label=oMT1308
     primer_bind     4429..4448
                     /label=oMT1135
     -35_signal      4494..4497
                     /label=-33
     primer_bind     4515..4534
                     /label=oMT100
     -10_signal      4520..4527
                     /label=-7
     RBS             4534..4559
                     /label=BT1311_RBS
     CDS             4560..5171
                     /codon_start=1
                     /label=VFA0359-Bt
                     /translation="MQKKLTRSQQKHLDIINAAKEEFIEFGFLAANMDRITSSAEVSKR
                     TLYRHFESKEVLFESVLTIINDSVNESISYHFDPNKSTEEQLTEIAYKEIDVLYKTYGI
                     ALARTIVMEFLRQPEMAKTLIQNIYSIRAITQWFRSAIEAKRLKDADPKLMTDVYVSLF
                     QGLFFWPQVMHLDLEPHGEELSQKIETLTTIFLQSYGVAE"
     CDS             4560..4562
                     /codon_start=1
                     /label=CmeR(NCTC11168)
                     /translation="M"
     gene            4560..4562
                     /gene="rpoD"
                     /gene_synonym="alt; ECK3057; JW3039"
                     /locus_tag="b3067"
                     /label=rpoD
                     /db_xref="EcoGene:EG10896"
                     /db_xref="GeneID:947567"
     CDS             4560..4562
                     /codon_start=1
                     /label=BreR-Bt
                     /translation="M"
     primer_bind     4829..4850
                     /label=internal seq primer
     primer_bind     4829..4850
                     /label=oMT379
     primer_bind     complement(5142..5166)
                     /label=oMT677
     primer_bind     complement(5149..5176)
                     /label=oMT1309
     CDS             5169..5171
                     /codon_start=1
                     /label=CmeR(NCTC11168)
                     /translation=""
     gene            5169..5171
                     /gene="rpoD"
                     /gene_synonym="alt; ECK3057; JW3039"
                     /locus_tag="b3067"
                     /label=rpoD
                     /db_xref="EcoGene:EG10896"
                     /db_xref="GeneID:947567"
     CDS             5169..5171
                     /codon_start=1
                     /label=BreR-Bt
                     /translation=""
     CDS             5169..5171
                     /label=CmeR(NCTC11168)
     gene            5169..5171
                     /label=rpoD
     CDS             5169..5171
                     /label=BreR-Bt
     misc_feature    5172..5175
                     /label=CAAT
     misc_feature    5172..5175
                     /label=problematic assembly region
     primer_bind     5182..5196
                     /label=oMT676
     misc_feature    5182..5187
                     /label=problematic assembly region
     terminator      5188..5248
                     /label=L3S2P21 Terminator
     primer_bind     complement(5227..5267)
                     /label=oMT1272
     primer_bind     5249..5276
                     /label=oMT1259
     primer_bind     complement(5374..5394)
                     /label=pNBU1_seq.REV
     misc_feature    complement(5423..5461)
                     /label=mmD660
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    5462..7204
                     /label=intN1 Construct PCR Product - intN1 Construct P
                     /label=intN1 Construct PCR Product - intN1 Construct PCR 
                     Product 2
                     /note="Geneious type: Inserted sequence"
     misc_feature    5462..6261
                     /label=Piece1
     misc_feature    5462..5491
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
     primer_bind     5462..5490
                     /label=mmD658
     primer_bind     5483..5503
                     /label=oMT113
     misc_feature    5492..5821
                     /label=attN1 Region
     primer_bind     5492..5511
                     /label=intN1 Construct PCR Product (fwd) - pNBU2-PcfxA
                     /label=intN1 Construct PCR Product (fwd) - 
                     pNBU2-PcfxA-NanoLuc PCR Product
     misc_feature    5542..5557
                     /label=DR1
     misc_feature    5575..5589
                     /label=IR1
     misc_feature    5599..5613
                     /label=IR2
     misc_feature    5619..5632
                     /label=CC Region
     misc_feature    5631
                     /label=G-->C
     misc_feature    5631
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     misc_feature    5632
                     /label='-1'
     misc_feature    5633
                     /label='+1'
     misc_feature    5650..5665
                     /label=DR2
     primer_bind     complement(5688..5710)
                     /label=oMT114
     misc_feature    5774..5790
                     /label=DR3
     CDS             5837..6261
                     /codon_start=1
                     /transl_table=11
                     /gene="intN1"
                     /product="IntN1"
                     /label=intN1
                     /label=intN1 CDS
                     /note="NBU1 integrase"
                     /db_xref="GI:8308020"
                     /protein_id="AAF74437.1"
                     /translation="MKVTFIIKKAAKRYDTESMATIYVRFRNGRQLDSVAPTQLAINPN
                     LWDDKDECVKTKAVCNEEMRTHINEEIRQLKTYIEKVYQQEKEAIDKEWLKTTLDKFYH
                     PEKYFLPEEVVIKPTIGELFDEFLNKHPLSEVRKKNF"
     gene            5837..6261
                     /gene="intN1"
                     /label=intN1
                     /label=intN1 gene
     misc_feature    complement(6231..6261)
                     /label=mmD659
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    6232..7204
                     /label=Piece2
     CDS             6232..7174
                     /codon_start=1
                     /transl_table=11
                     /gene="intN1"
                     /product="IntN1"
                     /label=intN1
                     /label=intN1 CDS
                     /note="NBU1 integrase"
                     /db_xref="GI:8308020"
                     /protein_id="AAF74437.1"
                     /translation="MVGSTKEKFPGCQKSLTAL*TICKGYKERTKGLYP*CGFGNT*HA
                     SGYVGFLSERIPVL*TLPEHL*SHSRKEDTTAQKQKHADRLFFKNTHILPVVLR*QTHH
                     KQTFRQVSDRGVYIWYTLLYNTRRKGQDF*CRPFCHPTTGNTEGYIHISDTDRMQGERP
                     VPNDQTKCGQ*SHRIYSQENQRGESGYGTCST*RQSERNP*TLQRI*GKTVAVHIRAKV
                     Q*CHKKDIQISWS*PHRNNLRPVDAQRNQTTYL*SGKQPSGKTYVYRQYL*KSERPEPC
                     FRTVGTQGGKQSFQTIQGY*RRNEERPCKTTGL"
     gene            6232..7174
                     /gene="intN1"
                     /label=intN1
                     /label=intN1 gene
     misc_feature    6232..6261
                     /label=Piece2
     misc_feature    6232..6261
                     /label=Ligation
     primer_bind     6232..6251
                     /label=intN1 Construct PCR Product 2 (fwd)
     misc_feature    complement(6237..6261)
                     /label=intN1 Construct PCR Product (rev)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(7150..7176)
                     /label=intN1 Construct PCR Product 2 (rev) - pNBU2-Pcf
                     /label=intN1 Construct PCR Product 2 (rev) - 
                     pNBU2-PcfxA-NanoLuc PCR Product
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    7166
                     /label=T
                     /note="Geneious type: Editing History Replacement"
     misc_feature    complement(7168..7204)
                     /label=mmD661
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    7168
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    7175..7204
                     /label=HOMOLOGY
     misc_feature    7177..7204
                     /label=CTTGTAGTTCGTTTAATTCGCTTTTCGTTTTTACGAATTTAACGAAT
                     /label=CTTGTAGTTCGTTTAATTCGCTTTTCGTTTTTACGAATTTAACGAATTGA 
                     ATATCATTGTTTTACGATTGATTTTCATTATATATTTGCGCCACTATCCTAATCGGGG 
                     TAGTGGCTTTTCGTTTATAATACTGCCGAAACGGGAA
                     /note="Geneious type: Editing History Replacement"
ORIGIN
        1 agagtagcga tattaaaaga atccgatgag aaaagactaa tatttatcta tccattcagt
       61 ttgatttttc aggactttac atcgtcctga aagtatttgt tggtaccggt accgaggacg
      121 cgtaaacatt tacagttgca tgtggcctat tgtttttagc cgttaaatat tttataacta
      181 ttaaatagcg atacaaattg ttcgaaacta atattgttta tatcatatat tctcgcatgt
      241 tttaaagctt tattaaattg attttttgta aacagttttt cgtactcttt gttaacccat
      301 ttcattacaa aagtttcata tttttttctc tctttaaatg ccatttttgc tggctttctt
      361 tttaatacaa ttaatgtgct atccacttta ggttttggat ggaaataata cctaggaatt
      421 tttgctaata tagaaatatc tacctctgcc attaacagca atgctagtga tctgtttgta
      481 tctaataaca ttttagcaaa accatattcc actattaaat aacttattgt ggctgaactt
      541 tcaaaaacaa tttttcgaat tatatttgtg cttatgttgt aaggtatgct gccaaatatt
      601 ttatatggat tgtggctagg aaatgtaaat ttcagtatat catcatttac tatttgatag
      661 ttaggataat ttaagagctt attacgagtt acctcacata atttagaatc aatttctatc
      721 gccgttacaa aattacatct ctttaccaat ccagcagtaa aatgaccttt ccctgcacct
      781 atttcaaaga tgttatcttt ttcatctaaa cttatgcaat tcattatttt ttctatgtga
      841 tattttgaag taataaaatt ttgactatct tttatattta ctttgttcat tataacctct
      901 ccttaattta ttgcatctct tttcgaatat ttatgttttt tgagaaaaga acgtactcat
      961 ggttcatccc gatatgcgta tcggtctgta tatcagcaac tttctatgtg tttcaactac
     1021 aatagtcatc tattctcatc tttctgagtc caccccctgc aaagcccctc tttacgacat
     1081 aaaaattcgg tcggaaaagg tatgcaaaag atgtttctct ctttaagaga aactcttcgg
     1141 gatgcaaaaa tatgaaaata actccaattc accaaattat atagcgactt ttttacaaaa
     1201 tgctaaaatt tgttgatttc cgtcaagcaa ttgttgagca aaaatgtctt ttacgataaa
     1261 atgatacctc aatatcaact gtttagcaaa acgatatttc tcttaaagag agaaacacct
     1321 ttttgttcac caatccccga cttttaatcc cgcggccatg attgaaaaag gaagagtatg
     1381 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1441 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     1501 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     1561 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     1621 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     1681 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     1741 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     1801 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     1861 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     1921 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     1981 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2041 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtcacgc
     2101 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2161 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     2221 ctgattaagc attggtaact gtcagaccaa gtttactcat aacgcgtcaa ttcgaggggg
     2281 atcaattccg tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc
     2341 ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg
     2401 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct
     2461 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
     2521 ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata
     2581 atgaccccga agcagggtta tgcagcggaa aacggaattg atccggccac gatgcgtccg
     2641 gcgtagagga tctgaagatc agcagttcaa cctgttgata gtacgtacta agctctcatg
     2701 tttcacgtac taagctctca tgtttaacgt actaagctct catgtttaac gaactaaacc
     2761 ctcatggcta acgtactaag ctctcatggc taacgtacta agctctcatg tttcacgtac
     2821 taagctctca tgtttgaaca ataaaattaa tataaatcag caacttaaat agcctctaag
     2881 gttttaagtt ttataagaaa aaaaagaata tataaggctt ttaaagcttt taaggtttaa
     2941 cggttgtgga caacaagcca gggatgtaac gcactgagaa gcccttagag cctctcaaag
     3001 caattttgag tgacacagga acacttaacg gctgacatgg gaattcccct ccaccgcggt
     3061 ggattaatgc ggctgcaatt tttttgggcg gggccgccca aaaaaatcct agcaccctgc
     3121 agggtctccc gctttacaaa gaaaattcga caaactgtta tttttctatc tatttaaatg
     3181 tacacccgtt tgtttacttt actttagtta tgtacctttg tcggcaatgt acacccgaaa
     3241 gtttactttc aaagataatt aagttaaggg ttttagagct agaaatagca agttaaaata
     3301 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttggatccct
     3361 cggtaccaaa ttccagaaaa gagacgctga aaagcgtctt ttttcgtttt ggtccgcggc
     3421 cgctgctgtc ataggccggt tgtccggtag tccggatgat ttaaggaaga tttctcttct
     3481 tttttcactt tctccgaaca acctccacct taacttaatt atctttgcag tagctgtcac
     3541 cggatgtgct ttccggtctg atgagtccgt gaggacgaaa cagcctctac aaataatttt
     3601 gtttaacgca ttttaaaata aaataaatta tttatttaat taaacgaata tggtttttac
     3661 tctggaagat tttgttggcg attggcgtca gaccgcgggt tataatttgg atcaagtcct
     3721 ggaacagggt ggcgtaagct ctctgttcca gaacctgggt gtgagcgtga cgccgattca
     3781 gcgcatcgtt ctgtccggcg agaacggtct gaaaattgat attcatgtga tcatcccgta
     3841 cgaaggcctg agcggtgacc aaatgggtca aatcgagaaa atctttaaag tcgtctaccc
     3901 agttgacgat caccacttca aggttatctt gcattacggt acgctggtga ttgatggtgt
     3961 gaccccgaat atgattgact atttcggccg tccgtatgaa ggcattgccg tttttgacgg
     4021 taaaaagatc accgtcaccg gtaccctgtg gaatggcaat aagattattg acgagcgtct
     4081 gattaacccg gacggcagcc tgctgttccg cgtgaccatc aacggtgtca cgggttggcg
     4141 tctgtgcgag cgcatcctgg cataatgaac tgggatccct cggtaccaaa ttttcgaaaa
     4201 aagacgctga aaagcgtctt ttttcgtttt ggtccgcggc cgccggccgc tgatctggaa
     4261 gaagcaatga aagctgctgt taagtctccg aatcaggtat tgttcctgac aggtgtattc
     4321 ccatccggta aacgcggata ctttgcagtt gatctgactc aggaataaat tataaattaa
     4381 ggtaagaaga ttgtaggata agctaatgaa atagaaaaag gatgccgtca cacaacttgt
     4441 cggcattctt ttttgtttta ttagttgaaa atatagtgaa aaagttgcct aaatatgtat
     4501 gttaacaaat tatttgtcgt aactttgcac tccaaatctg tttttaacat atggcactaa
     4561 tgcagaaaaa attgactcgt tctcagcaga aacacttgga catcatcaac gctgctaaag
     4621 aagaattcat cgaattcggt ttcttggctg ctaacatgga ccgtatcact tcttctgctg
     4681 aagtatctaa acgtactttg taccgtcact tcgaatctaa agaagtattg ttcgaatctg
     4741 tattgactat catcaacgac tctgtaaacg aatctatctc ttaccacttc gacccgaaca
     4801 aatctactga agaacagttg actgaaatcg cttacaaaga aatcgacgta ttgtacaaaa
     4861 cttacggtat cgctttggct cgtactatcg taatggaatt cttgcgtcag ccggaaatgg
     4921 ctaaaacttt gatccagaac atctactcta tccgtgctat cactcagtgg ttccgttctg
     4981 ctatcgaagc taaacgtttg aaagacgctg acccgaaatt gatgactgac gtatacgtat
     5041 ctttgttcca gggtttgttc ttctggccgc aggtaatgca cttggacttg gaaccgcacg
     5101 gtgaagaatt gtctcagaaa atcgaaactt tgactactat cttcttgcag tcttacggtg
     5161 tagctgaata acaatgaatg ggctttcctc ggtaccaaat tccagaaaag aggcctcccg
     5221 aaaggggggc cttttttcgt tttggtccct aattgcctat cttccagtga tggaacagca
     5281 tttgtgcatt ggctgcaaca atcagcctta cttgtgcctg ttctatttcc gaaccgaccg
     5341 cttgtatgaa tccatcaaaa ttcgttttct ctatgttgga ttccttgttg ctcatattgt
     5401 gatgataatt tctacaaata tagtcattgg taactatcta tgaaactgtt tgatactttt
     5461 actcatcgga ttcttttaat atcgctactc tctacgttca accaaaagaa ataatgactt
     5521 actgctatat tttttgcacg tgtggggaaa atgtggggaa aattcaagca aaagaaaaag
     5581 ctaagtattg aactatcaaa tacttagctt tctttcttgt acccagaccc cgcatttgaa
     5641 ataattaaag tggggaaaat gtgggtaaaa agaaaaatgc ggaaaaacgc cacaattaca
     5701 ctgtatttca atatgttata atcctattaa attttaatcc aagtttaatc gaattgcaaa
     5761 atatttagca gatgtgggga aaatgctggg gaaaatattt atatttgcag cagagtaaaa
     5821 ttcaacctga aagtctatga aagtaacctt tatcattaaa aaagcagcca aacgatatga
     5881 tacagaatcc atggctacaa tctatgtccg ttttagaaac ggaaggcagt tagactccgt
     5941 tgctcctact cagttagcca tcaatcccaa tctatgggat gataaagacg aatgtgtaaa
     6001 aacgaaagct gtctgcaatg aagaaatgcg tacccatata aatgaagaga tacgccagtt
     6061 gaaaacctat atcgagaagg tatatcaaca agaaaaggaa gcaatagaca aagaatggct
     6121 aaaaacaaca cttgataaat tttaccatcc tgaaaaatat tttttgccgg aggaagtggt
     6181 tatcaagcct accattggag aactattcga tgaatttcta aacaagcacc ctttgtcgga
     6241 agtacgaaag aaaaatttcc gggttgtcaa aagagcctta ctgcgttatg aactatatgt
     6301 aagggctaca aagagaggac aaaagggctt tatccttgat gtggatttgg taacacctga
     6361 cacgcttcgg gatatgtggg atttctttca gaacgaatac cagtattatg aactttaccc
     6421 gagcatttat gaagccattc ccgaaaagag gacaccacag cccagaagca aaaacacgct
     6481 gatagactgt ttttcaagaa tacgcacatt cttcctgtgg tgcttcgata acaaacgcac
     6541 cacaaacaga cctttcgaca agtttccgat agaggagtgt acatatggta caccttatta
     6601 tataacactc gaagaaaggg acaggatttt taatgcagac ctttctgcca ccccacaact
     6661 ggcaatacag agggatatat tcatatttca gacactgata ggatgcaggg tgagcgacct
     6721 gtaccgaatg accaaactaa atgtggtcaa tgaagccata gaatatattc ccaagaaaac
     6781 caaagagggg aatccggtta cggtacgtgt tccacttaac gacaaagcga aagaaatcct
     6841 tgaacgctac aaagaatatg agggaaaact gttgccgttc atatccgagc aaaagtacaa
     6901 tgatgccata aaaaagatat tcaaattagc tggagttgac cgcatcgtaa caatcttaga
     6961 cccgttgacg cacaacgaaa tcaaacgacc tatttatgaa gtggcaagca gccatctggc
     7021 aagacgtacg tttatcggca atatctataa aaaagtgaaa gacccgaacc ttgtttccgc
     7081 actgtcggga cacaaggagg gaagcaaagc tttcagacga tacagggata ttgacgaaga
     7141 aatgaagaaa gaccttgtaa aactactgga ctgataaaag tatcaaacag tttcatagat
     7201 agtt
//
