LOCUS       Exported                7200 bp ds-DNA     circular SYN 15-NOV-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7200)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 20, 2020 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7200
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..30
                     /label=mmD661
     misc_feature    1
                     /label=origin
     misc_recomb     118..2262
                     /label=NluI fragment from pKNOCK
                     /note="NluI fragment from pKNOCK"
     CDS             complement(156..890)
                     /label=ermG
                     /note="ermG"
     CDS             1378..2239
                     /label=beta-lactamase from pBR322
                     /note="beta-lactamase from pBR322"
     primer_bind     2089..2106
                     /label=oMT1184
     primer_bind     complement(2089..2106)
                     /label=oMT1189
     CDS             2097
                     /label=beta-lactamase from pBR322
                     /note="beta-lactamase from pBR322"
     misc_recomb     2097
                     /label=NluI fragment from pKNOCK
                     /note="NluI fragment from pKNOCK"
     misc_feature    2097
                     /label=t2096a
     misc_recomb     2277..3062
                     /label=RP4 oriT/RSK oriV
                     /note="RP4 oriT/RSK oriV"
     primer_bind     2968..2987
                     /label=oMT25
     primer_bind     2968..2987
                     /label=pNBU1_seq.FOR
     misc_feature    3062
                     /label=3062 mutation from ""g"" to ""t""
                     /note="Mutated in pMT1fix2, but will not fix, since it's at
                     the backbone of the vector and is not CDS or a region with 
                     important UTR."
     misc_feature    3063..3122
                     /label=pNBU2-Lac-dCas9-NL3 PCR Product - pNBU2-Lac-dCas9-
                     /note="Geneious type: Inserted sequence"
     terminator      3078..3107
                     /label=tL17 terminator
                     /label=tL17
     primer_bind     complement(3099..3122)
                     /label=oMT912
     misc_feature    3103..3122
                     /label=Feature 84
     primer_bind     3123..3159
                     /label=oMT1293
     misc_feature    3130..3133
                     /label=CGCT
     promoter        3134..3176
                     /label=cfxA_promoter
     misc_feature    3177..3185
                     /label=AANGTANAC
     misc_feature    3186..3191
                     /label=N6
     misc_feature    3189..3192
                     /label=-33
     misc_feature    3192..3200
                     /label=GTNTACNTT
     -35_signal      3193..3198
                     /label=-35
     promoter        3201..3212
                     /label=PcfxA
     misc_feature    3213..3220
                     /label=-7
     misc_feature    3222..3225
                     /label=4bp-2
     primer_bind     complement(3223..3259)
                     /label=oMT916
     misc_feature    3225
                     /label=+1
     misc_feature    3226..3234
                     /label=AANGTANAC
     misc_feature    3235..3240
                     /label=N6
     misc_feature    3241..3249
                     /label=GTNTACNTT
     -35_signal      3242..3247
                     /label=-35
     misc_feature    3250..3269
                     /label=sgRNA-12
     misc_feature    3270..3311
                     /label=chimera tracrRNA
     primer_bind     3270..3303
                     /label=oMT915
     misc_feature    3316..3352
                     /label=S. pyogenes terminator
     primer_bind     complement(3333..3437)
                     /label=oMT1279
     misc_feature    3353..3411
                     /label=ECK120033736
     misc_feature    3354..3358
                     /label=Amp up this region
     misc_feature    3411
                     /label=CGCG
     misc_feature    3412..3419
                     /label=L3S2P56
     misc_feature    3412..3413
                     /label=Restriction Sites
     primer_bind     3419..3545
                     /label=oMT914
     misc_feature    3420..3503
                     /label=PBF2884
     primer_bind     3420..3439
                     /label=oMT1006
     primer_bind     3420..3438
                     /label=oMT1394
     misc_feature    3462..3466
                     /label=-60
     primer_bind     complement(3470..3498)
                     /label=oMT1116
     misc_feature    3473..3477
                     /label=-50
     primer_bind     3479..3503
                     /label=oMT1114
     primer_bind     complement(3480..3503)
                     /label=oMT1064
     misc_feature    3492..3495
                     /label=-33
     misc_feature    3496..3503
                     /label=20bp
     misc_feature    3501..3502
                     /label=PAM
     misc_feature    3504..3523
                     /label=sgRNA-12
     misc_feature    3528..3602
                     /label=RiboJ00
     terminator      3603..3645
                     /label=rpiL*
                     /note="rpiL* termninator"
     CDS             3646..4161
                     /codon_start=1
                     /label=Luciferase Reporter
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     3646..3670
                     /label=oMT41
     primer_bind     complement(3657..3676)
                     /label=oMT386
     primer_bind     3934..3953
                     /label=oMT399
     misc_feature    4133..5183
                     /label=problematic assembly region
     primer_bind     complement(4140..4165)
                     /label=oMT1343
     primer_bind     complement(4143..4161)
                     /label=oMT1307
     misc_feature    4162..5167
                     /label=Amp up this region
     primer_bind     4162..4272
                     /label=oMT1344
     misc_feature    4162..4246
                     /label=Amp up this region
     misc_feature    4162..4165
                     /label=TGAA
     misc_feature    4169..4239
                     /label=L3S2P56
     misc_feature    4232..4233
                     /label=Restriction Sites
     promoter        4247..4529
                     /label=BT1311_promoter
     primer_bind     4425..4444
                     /label=oMT1135
     -35_signal      4490..4493
                     /label=-33
     primer_bind     4511..4530
                     /label=oMT100
     -10_signal      4516..4523
                     /label=-7
     RBS             4530..4555
                     /label=BT1311_RBS
     CDS             4556..5167
                     /codon_start=1
                     /label=VFA0359-Bt
                     /translation="MQKKLTRSQQKHLDIINAAKEEFIEFGFLAANMDRITSSAEVSKR
                     TLYRHFESKEVLFESVLTIINDSVNESISYHFDPNKSTEEQLTEIAYKEIDVLYKTYGI
                     ALARTIVMEFLRQPEMAKTLIQNIYSIRAITQWFRSAIEAKRLKDADPKLMTDVYVSLF
                     QGLFFWPQVMHLDLEPHGEELSQKIETLTTIFLQSYGVAE"
     CDS             4556..4558
                     /codon_start=1
                     /label=BreR-Bt
                     /translation="M"
     gene            4556..4558
                     /gene="rpoD"
                     /gene_synonym="alt; ECK3057; JW3039"
                     /locus_tag="b3067"
                     /label=rpoD
                     /db_xref="EcoGene:EG10896"
                     /db_xref="GeneID:947567"
     CDS             4556..4558
                     /codon_start=1
                     /label=CmeR(NCTC11168)
                     /translation="M"
     primer_bind     4825..4846
                     /label=internal seq primer
     primer_bind     4825..4846
                     /label=oMT379
     primer_bind     complement(5138..5162)
                     /label=oMT677
     primer_bind     complement(5145..5172)
                     /label=oMT1309
     CDS             5165..5167
                     /label=BreR-Bt
     gene            5165..5167
                     /label=rpoD
     CDS             5165..5167
                     /label=CmeR(NCTC11168)
     CDS             5165..5167
                     /codon_start=1
                     /label=BreR-Bt
                     /translation=""
     gene            5165..5167
                     /gene="rpoD"
                     /gene_synonym="alt; ECK3057; JW3039"
                     /locus_tag="b3067"
                     /label=rpoD
                     /db_xref="EcoGene:EG10896"
                     /db_xref="GeneID:947567"
     CDS             5165..5167
                     /codon_start=1
                     /label=CmeR(NCTC11168)
                     /translation=""
     misc_feature    5168..5177
                     /label=problematic assembly region
     misc_feature    5168..5171
                     /label=CAAT
     misc_feature    5168..5171
                     /label=problematic assembly region
     primer_bind     5178..5192
                     /label=oMT676
     misc_feature    5178..5183
                     /label=problematic assembly region
     misc_feature    5178..5183
                     /label=Feature 69
     terminator      5184..5244
                     /label=L3S2P21 Terminator
     primer_bind     complement(5223..5263)
                     /label=oMT1272
     primer_bind     5245..5272
                     /label=oMT1259
     primer_bind     complement(5370..5390)
                     /label=pNBU1_seq.REV
     misc_feature    complement(5419..5457)
                     /label=mmD660
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    5458..7200
                     /label=intN1 Construct PCR Product - intN1 Construct P
                     /label=intN1 Construct PCR Product - intN1 Construct PCR 
                     Product 2
                     /note="Geneious type: Inserted sequence"
     misc_feature    5458..6257
                     /label=Piece1
     misc_feature    5458..5487
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
     primer_bind     5458..5486
                     /label=mmD658
     primer_bind     5479..5499
                     /label=oMT113
     misc_feature    5488..5817
                     /label=attN1 Region
     primer_bind     5488..5507
                     /label=intN1 Construct PCR Product (fwd) - pNBU2-PcfxA
                     /label=intN1 Construct PCR Product (fwd) - 
                     pNBU2-PcfxA-NanoLuc PCR Product
     misc_feature    5538..5553
                     /label=DR1
     misc_feature    5571..5585
                     /label=IR1
     misc_feature    5595..5609
                     /label=IR2
     misc_feature    5615..5628
                     /label=CC Region
     misc_feature    5627
                     /label=G-->C
     misc_feature    5627
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     misc_feature    5628
                     /label='-1'
     misc_feature    5629
                     /label='+1'
     misc_feature    5646..5661
                     /label=DR2
     primer_bind     complement(5684..5706)
                     /label=oMT114
     misc_feature    5770..5786
                     /label=DR3
     CDS             5833..6257
                     /codon_start=1
                     /transl_table=11
                     /gene="intN1"
                     /product="IntN1"
                     /label=intN1
                     /label=intN1 CDS
                     /note="NBU1 integrase"
                     /db_xref="GI:8308020"
                     /protein_id="AAF74437.1"
                     /translation="MKVTFIIKKAAKRYDTESMATIYVRFRNGRQLDSVAPTQLAINPN
                     LWDDKDECVKTKAVCNEEMRTHINEEIRQLKTYIEKVYQQEKEAIDKEWLKTTLDKFYH
                     PEKYFLPEEVVIKPTIGELFDEFLNKHPLSEVRKKNF"
     gene            5833..6257
                     /gene="intN1"
                     /label=intN1
                     /label=intN1 gene
     primer_bind     complement(6049..6076)
                     /label=oMT157
     primer_bind     6049..6076
                     /label=oMT156
     misc_feature    complement(6227..6257)
                     /label=mmD659
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    6228..7200
                     /label=Piece2
     CDS             6228..7170
                     /codon_start=1
                     /transl_table=11
                     /gene="intN1"
                     /product="IntN1"
                     /label=intN1
                     /label=intN1 CDS
                     /note="NBU1 integrase"
                     /db_xref="GI:8308020"
                     /protein_id="AAF74437.1"
                     /translation="MVGSTKEKFPGCQKSLTAL*TICKGYKERTKGLYP*CGFGNT*HA
                     SGYVGFLSERIPVL*TLPEHL*SHSRKEDTTAQKQKHADRLFFKNTHILPVVLR*QTHH
                     KQTFRQVSDRGVYIWYTLLYNTRRKGQDF*CRPFCHPTTGNTEGYIHISDTDRMQGERP
                     VPNDQTKCGQ*SHRIYSQENQRGESGYGTCST*RQSERNP*TLQRI*GKTVAVHIRAKV
                     Q*CHKKDIQISWS*PHRNNLRPVDAQRNQTTYL*SGKQPSGKTYVYRQYL*KSERPEPC
                     FRTVGTQGGKQSFQTIQGY*RRNEERPCKTTGL"
     gene            6228..7170
                     /gene="intN1"
                     /label=intN1
                     /label=intN1 gene
     misc_feature    6228..6257
                     /label=Piece2
     misc_feature    6228..6257
                     /label=Ligation
     primer_bind     6228..6247
                     /label=intN1 Construct PCR Product 2 (fwd)
     misc_feature    complement(6233..6257)
                     /label=intN1 Construct PCR Product (rev)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(7146..7172)
                     /label=intN1 Construct PCR Product 2 (rev) - pNBU2-Pcf
                     /label=intN1 Construct PCR Product 2 (rev) - 
                     pNBU2-PcfxA-NanoLuc PCR Product
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    7162
                     /label=T
                     /note="Geneious type: Editing History Replacement"
     misc_feature    complement(7164..7200)
                     /label=mmD661
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    7164
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    7171..7200
                     /label=HOMOLOGY
     misc_feature    7173..7200
                     /label=CTTGTAGTTCGTTTAATTCGCTTTTCGTTTTTACGAATTTAACGAAT
                     /label=CTTGTAGTTCGTTTAATTCGCTTTTCGTTTTTACGAATTTAACGAATTGA 
                     ATATCATTGTTTTACGATTGATTTTCATTATATATTTGCGCCACTATCCTAATCGGGG 
                     TAGTGGCTTTTCGTTTATAATACTGCCGAAACGGGAA
                     /note="Geneious type: Editing History Replacement"
ORIGIN
        1 agagtagcga tattaaaaga atccgatgag aaaagactaa tatttatcta tccattcagt
       61 ttgatttttc aggactttac atcgtcctga aagtatttgt tggtaccggt accgaggacg
      121 cgtaaacatt tacagttgca tgtggcctat tgtttttagc cgttaaatat tttataacta
      181 ttaaatagcg atacaaattg ttcgaaacta atattgttta tatcatatat tctcgcatgt
      241 tttaaagctt tattaaattg attttttgta aacagttttt cgtactcttt gttaacccat
      301 ttcattacaa aagtttcata tttttttctc tctttaaatg ccatttttgc tggctttctt
      361 tttaatacaa ttaatgtgct atccacttta ggttttggat ggaaataata cctaggaatt
      421 tttgctaata tagaaatatc tacctctgcc attaacagca atgctagtga tctgtttgta
      481 tctaataaca ttttagcaaa accatattcc actattaaat aacttattgt ggctgaactt
      541 tcaaaaacaa tttttcgaat tatatttgtg cttatgttgt aaggtatgct gccaaatatt
      601 ttatatggat tgtggctagg aaatgtaaat ttcagtatat catcatttac tatttgatag
      661 ttaggataat ttaagagctt attacgagtt acctcacata atttagaatc aatttctatc
      721 gccgttacaa aattacatct ctttaccaat ccagcagtaa aatgaccttt ccctgcacct
      781 atttcaaaga tgttatcttt ttcatctaaa cttatgcaat tcattatttt ttctatgtga
      841 tattttgaag taataaaatt ttgactatct tttatattta ctttgttcat tataacctct
      901 ccttaattta ttgcatctct tttcgaatat ttatgttttt tgagaaaaga acgtactcat
      961 ggttcatccc gatatgcgta tcggtctgta tatcagcaac tttctatgtg tttcaactac
     1021 aatagtcatc tattctcatc tttctgagtc caccccctgc aaagcccctc tttacgacat
     1081 aaaaattcgg tcggaaaagg tatgcaaaag atgtttctct ctttaagaga aactcttcgg
     1141 gatgcaaaaa tatgaaaata actccaattc accaaattat atagcgactt ttttacaaaa
     1201 tgctaaaatt tgttgatttc cgtcaagcaa ttgttgagca aaaatgtctt ttacgataaa
     1261 atgatacctc aatatcaact gtttagcaaa acgatatttc tcttaaagag agaaacacct
     1321 ttttgttcac caatccccga cttttaatcc cgcggccatg attgaaaaag gaagagtatg
     1381 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1441 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     1501 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     1561 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     1621 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     1681 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     1741 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     1801 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     1861 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     1921 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     1981 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2041 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtcacgc
     2101 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2161 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     2221 ctgattaagc attggtaact gtcagaccaa gtttactcat aacgcgtcaa ttcgaggggg
     2281 atcaattccg tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc
     2341 ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg
     2401 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct
     2461 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
     2521 ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata
     2581 atgaccccga agcagggtta tgcagcggaa aacggaattg atccggccac gatgcgtccg
     2641 gcgtagagga tctgaagatc agcagttcaa cctgttgata gtacgtacta agctctcatg
     2701 tttcacgtac taagctctca tgtttaacgt actaagctct catgtttaac gaactaaacc
     2761 ctcatggcta acgtactaag ctctcatggc taacgtacta agctctcatg tttcacgtac
     2821 taagctctca tgtttgaaca ataaaattaa tataaatcag caacttaaat agcctctaag
     2881 gttttaagtt ttataagaaa aaaaagaata tataaggctt ttaaagcttt taaggtttaa
     2941 cggttgtgga caacaagcca gggatgtaac gcactgagaa gcccttagag cctctcaaag
     3001 caattttgag tgacacagga acacttaacg gctgacatgg gaattcccct ccaccgcggt
     3061 ggattaatgc ggctgcaatt tttttgggcg gggccgccca aaaaaatcct agcaccctgc
     3121 agggtctccc gctttacaaa gaaaattcga caaactgtta tttttctatc tatttaaatg
     3181 tacacccgtt tgtttacttt actttagtta tgtacctttg tcggcaatgt acacccgaaa
     3241 gtttactttc aaagatactt atgttagaag ttttagagct agaaatagca agttaaaata
     3301 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttggatccaa
     3361 cgcatgagaa agcccccgga agatcacctt ccgggggctt ttttattgcg cgcggccgct
     3421 gctgtcatag gccggttgtc cggtagtccg gatgatttaa ggaagatttc tcttcttttt
     3481 tcactttctc cgaacaacct ccattctaac ataagtatct ttgcagtagc tgtcaccgga
     3541 tgtgctttcc ggtctgatga gtccgtgagg acgaaacagc ctctacaaat aattttgttt
     3601 aacgcatttt aaaataaaat aaattattta tttaattaaa cgaatatggt ttttactctg
     3661 gaagattttg ttggcgattg gcgtcagacc gcgggttata atttggatca agtcctggaa
     3721 cagggtggcg taagctctct gttccagaac ctgggtgtga gcgtgacgcc gattcagcgc
     3781 atcgttctgt ccggcgagaa cggtctgaaa attgatattc atgtgatcat cccgtacgaa
     3841 ggcctgagcg gtgaccaaat gggtcaaatc gagaaaatct ttaaagtcgt ctacccagtt
     3901 gacgatcacc acttcaaggt tatcttgcat tacggtacgc tggtgattga tggtgtgacc
     3961 ccgaatatga ttgactattt cggccgtccg tatgaaggca ttgccgtttt tgacggtaaa
     4021 aagatcaccg tcaccggtac cctgtggaat ggcaataaga ttattgacga gcgtctgatt
     4081 aacccggacg gcagcctgct gttccgcgtg accatcaacg gtgtcacggg ttggcgtctg
     4141 tgcgagcgca tcctggcata atgaactggg atccctcggt accaaatttt cgaaaaaaga
     4201 cgctgaaaag cgtctttttt cgttttggtc cgcggccgcc ggccgctgat ctggaagaag
     4261 caatgaaagc tgctgttaag tctccgaatc aggtattgtt cctgacaggt gtattcccat
     4321 ccggtaaacg cggatacttt gcagttgatc tgactcagga ataaattata aattaaggta
     4381 agaagattgt aggataagct aatgaaatag aaaaaggatg ccgtcacaca acttgtcggc
     4441 attctttttt gttttattag ttgaaaatat agtgaaaaag ttgcctaaat atgtatgtta
     4501 acaaattatt tgtcgtaact ttgcactcca aatctgtttt taacatatgg cactaatgca
     4561 gaaaaaattg actcgttctc agcagaaaca cttggacatc atcaacgctg ctaaagaaga
     4621 attcatcgaa ttcggtttct tggctgctaa catggaccgt atcacttctt ctgctgaagt
     4681 atctaaacgt actttgtacc gtcacttcga atctaaagaa gtattgttcg aatctgtatt
     4741 gactatcatc aacgactctg taaacgaatc tatctcttac cacttcgacc cgaacaaatc
     4801 tactgaagaa cagttgactg aaatcgctta caaagaaatc gacgtattgt acaaaactta
     4861 cggtatcgct ttggctcgta ctatcgtaat ggaattcttg cgtcagccgg aaatggctaa
     4921 aactttgatc cagaacatct actctatccg tgctatcact cagtggttcc gttctgctat
     4981 cgaagctaaa cgtttgaaag acgctgaccc gaaattgatg actgacgtat acgtatcttt
     5041 gttccagggt ttgttcttct ggccgcaggt aatgcacttg gacttggaac cgcacggtga
     5101 agaattgtct cagaaaatcg aaactttgac tactatcttc ttgcagtctt acggtgtagc
     5161 tgaataacaa tgaatgggct ttcctcggta ccaaattcca gaaaagaggc ctcccgaaag
     5221 gggggccttt tttcgttttg gtccctaatt gcctatcttc cagtgatgga acagcatttg
     5281 tgcattggct gcaacaatca gccttacttg tgcctgttct atttccgaac cgaccgcttg
     5341 tatgaatcca tcaaaattcg ttttctctat gttggattcc ttgttgctca tattgtgatg
     5401 ataatttcta caaatatagt cattggtaac tatctatgaa actgtttgat acttttactc
     5461 atcggattct tttaatatcg ctactctcta cgttcaacca aaagaaataa tgacttactg
     5521 ctatattttt tgcacgtgtg gggaaaatgt ggggaaaatt caagcaaaag aaaaagctaa
     5581 gtattgaact atcaaatact tagctttctt tcttgtaccc agaccccgca tttgaaataa
     5641 ttaaagtggg gaaaatgtgg gtaaaaagaa aaatgcggaa aaacgccaca attacactgt
     5701 atttcaatat gttataatcc tattaaattt taatccaagt ttaatcgaat tgcaaaatat
     5761 ttagcagatg tggggaaaat gctggggaaa atatttatat ttgcagcaga gtaaaattca
     5821 acctgaaagt ctatgaaagt aacctttatc attaaaaaag cagccaaacg atatgataca
     5881 gaatccatgg ctacaatcta tgtccgtttt agaaacggaa ggcagttaga ctccgttgct
     5941 cctactcagt tagccatcaa tcccaatcta tgggatgata aagacgaatg tgtaaaaacg
     6001 aaagctgtct gcaatgaaga aatgcgtacc catataaatg aagagatacg ccagttgaaa
     6061 acctatatcg agaaggtata tcaacaagaa aaggaagcaa tagacaaaga atggctaaaa
     6121 acaacacttg ataaatttta ccatcctgaa aaatattttt tgccggagga agtggttatc
     6181 aagcctacca ttggagaact attcgatgaa tttctaaaca agcacccttt gtcggaagta
     6241 cgaaagaaaa atttccgggt tgtcaaaaga gccttactgc gttatgaact atatgtaagg
     6301 gctacaaaga gaggacaaaa gggctttatc cttgatgtgg atttggtaac acctgacacg
     6361 cttcgggata tgtgggattt ctttcagaac gaataccagt attatgaact ttacccgagc
     6421 atttatgaag ccattcccga aaagaggaca ccacagccca gaagcaaaaa cacgctgata
     6481 gactgttttt caagaatacg cacattcttc ctgtggtgct tcgataacaa acgcaccaca
     6541 aacagacctt tcgacaagtt tccgatagag gagtgtacat atggtacacc ttattatata
     6601 acactcgaag aaagggacag gatttttaat gcagaccttt ctgccacccc acaactggca
     6661 atacagaggg atatattcat atttcagaca ctgataggat gcagggtgag cgacctgtac
     6721 cgaatgacca aactaaatgt ggtcaatgaa gccatagaat atattcccaa gaaaaccaaa
     6781 gaggggaatc cggttacggt acgtgttcca cttaacgaca aagcgaaaga aatccttgaa
     6841 cgctacaaag aatatgaggg aaaactgttg ccgttcatat ccgagcaaaa gtacaatgat
     6901 gccataaaaa agatattcaa attagctgga gttgaccgca tcgtaacaat cttagacccg
     6961 ttgacgcaca acgaaatcaa acgacctatt tatgaagtgg caagcagcca tctggcaaga
     7021 cgtacgttta tcggcaatat ctataaaaaa gtgaaagacc cgaaccttgt ttccgcactg
     7081 tcgggacaca aggagggaag caaagctttc agacgataca gggatattga cgaagaaatg
     7141 aagaaagacc ttgtaaaact actggactga taaaagtatc aaacagtttc atagatagtt
//
