LOCUS       Exported                9402 bp ds-DNA     circular SYN 05-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 20, 2020 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9402
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..30
                     /label=mmD661
     misc_feature    1
                     /label=origin
     misc_recomb     118..2262
                     /label=NluI fragment from pKNOCK
                     /note="NluI fragment from pKNOCK"
     CDS             complement(156..890)
                     /label=ermG
                     /note="ermG"
     CDS             1378..2239
                     /label=beta-lactamase from pBR322
                     /note="beta-lactamase from pBR322"
     primer_bind     1837..1856
                     /label=oMT203
     primer_bind     complement(2089..2106)
                     /label=oMT1189
     primer_bind     2089..2106
                     /label=oMT1184
     misc_feature    2097
                     /label=t2096a
     misc_recomb     2277..3062
                     /label=RP4 oriT/RSK oriV
                     /note="RP4 oriT/RSK oriV"
     primer_bind     complement(2340..2359)
                     /label=oMT204
     primer_bind     2968..2987
                     /label=pNBU1_seq.FOR
     misc_feature    3062
                     /label=3062 mutation from ""g"" to ""t""
                     /note="Mutated in pMT1fix2, but will not fix, since it's at
                     the backbone of the vector and is not CDS or a region with 
                     important UTR."
     misc_feature    3063..3122
                     /label=pNBU2-Lac-dCas9-NL3 PCR Product - pNBU2-Lac-dCas9-
                     /note="Geneious type: Inserted sequence"
     terminator      3078..3107
                     /label=tL17 terminator
                     /label=tL17
     primer_bind     complement(3104..3133)
                     /label=oMT1185
     misc_feature    3123..3130
                     /label=L3S3P21
     misc_feature    3130..3133
                     /label=CGCT
     misc_feature    3134..3137
                     /label=Ascar
                     /label=nonstandard type: scar
     promoter        3138..3310
                     /label=P_aTc
     CDS             3311..3413
                     /label=M1
     terminator      3414..3474
                     /label=L3S3P41
     primer_bind     3469..3486
                     /label=oMT1436
     misc_feature    3475..3478
                     /label=Bscar
                     /label=nonstandard type: scar
     promoter        3479..3651
                     /label=P_aTc
     CDS             3652..3754
                     /label=M3
     terminator      3755..3825
                     /label=ECK120034435
     misc_feature    3826..3829
                     /label=Dscar
                     /label=nonstandard type: scar
     promoter        3830..3937
                     /label=PM1
     CDS             3938..4040
                     /label=M4
     terminator      4041..4107
                     /label=ECK120033736
     primer_bind     4104..4122
                     /label=oMT1437
     misc_feature    4108..4111
                     /label=Escar
                     /label=nonstandard type: scar
     promoter        4112..4219
                     /label=PM1
     CDS             4220..4322
                     /label=M3
     terminator      4323..4393
                     /label=ECK120034435
     misc_feature    4394..4397
                     /label=Fscar
                     /label=nonstandard type: scar
     promoter        4398..4513
                     /label=P_BA
     CDS             4514..4616
                     /label=M4
     terminator      4617..4683
                     /label=ECK120033736
     misc_feature    4684..4687
                     /label=Xscar
                     /label=nonstandard type: scar
     promoter        4688..4803
                     /label=P_BA
     CDS             4804..4906
                     /label=M1
     terminator      4907..4967
                     /label=L3S3P41
     misc_feature    4951
                     /label=extra T?
     primer_bind     4964..4981
                     /label=oMT1438
     misc_feature    4968..4971
                     /label=Cscar
                     /label=nonstandard type: scar
     misc_feature    4972..4975
                     /label=Ascar
                     /label=nonstandard type: scar
     promoter        4976..5083
                     /label=PM4
     primer_bind     5051..5071
                     /label=oMT1421
     primer_bind     5083..5101
                     /label=oMT1342
     misc_feature    5084..5158
                     /label=RiboJ00
     terminator      5085..5086
                     /label=rpiL*
                     /note="rpiL* termninator"
     terminator      5159..5201
                     /label=rpiL*
                     /note="rpiL* termninator"
     CDS             5202..5207
                     /codon_start=1
                     /label=Luciferase Reporter
                     /translation="M"
     misc_feature    5208..5211
                     /label=TGAA
     misc_feature    5215..5285
                     /label=L3S2P56
     misc_feature    5278..5279
                     /label=Restriction Sites
     misc_feature    5293..5296
                     /label=Bscar
                     /label=nonstandard type: scar
     promoter        5297..5404
                     /label=PM1
     primer_bind     5404..5422
                     /label=oMT1342
     misc_feature    5405..5479
                     /label=RiboJ00
     terminator      5406..5407
                     /label=rpiL*
                     /note="rpiL* termninator"
     terminator      5480..5522
                     /label=rpiL*
                     /note="rpiL* termninator"
     CDS             5523..5528
                     /codon_start=1
                     /label=Luciferase Reporter
                     /translation="M"
     misc_feature    5529..5532
                     /label=TGAA
     misc_feature    5536..5606
                     /label=L3S2P56
     misc_feature    5599..5600
                     /label=Restriction Sites
     misc_feature    5614..5617
                     /label=Dscar
                     /label=nonstandard type: scar
     promoter        5618..5725
                     /label=PM3
     primer_bind     5725..5743
                     /label=oMT1342
     misc_feature    5726..5800
                     /label=RiboJ00
     terminator      5727..5728
                     /label=rpiL*
                     /note="rpiL* termninator"
     terminator      5801..5843
                     /label=rpiL*
                     /note="rpiL* termninator"
     primer_bind     complement(5839..5868)
                     /label=oMT641
     CDS             5844..6359
                     /codon_start=1
                     /label=Luciferase Reporter
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     5844..5868
                     /label=oMT41
     primer_bind     6132..6151
                     /label=oMT399
     primer_bind     complement(6338..6363)
                     /label=oMT1343
     primer_bind     6360..6474
                     /label=oMT1344
     misc_feature    6360..6363
                     /label=TGAA
     misc_feature    6367..6437
                     /label=L3S2P56
     misc_feature    6430..6431
                     /label=Restriction Sites
     misc_feature    6445..6448
                     /label=Cscar
                     /label=nonstandard type: scar
     promoter        6449..6731
                     /label=BT1311_promoter
     -35_signal      6692..6695
                     /label=-33
     primer_bind     6713..6732
                     /label=oMT100
     -10_signal      6718..6725
                     /label=-7
     RBS             6732..6757
                     /label=BT1311_RBS
     CDS             6758..7369
                     /codon_start=1
                     /label=VFA0359-Bt
                     /translation="MQKKLTRSQQKHLDIINAAKEEFIEFGFLAANMDRITSSAEVSKR
                     TLYRHFESKEVLFESVLTIINDSVNESISYHFDPNKSTEEQLTEIAYKEIDVLYKTYGI
                     ALARTIVMEFLRQPEMAKTLIQNIYSIRAITQWFRSAIEAKRLKDADPKLMTDVYVSLF
                     QGLFFWPQVMHLDLEPHGEELSQKIETLTTIFLQSYGVAE"
     CDS             6758..6760
                     /codon_start=1
                     /label=CmeR(NCTC11168)
                     /translation="M"
     gene            6758..6760
                     /gene="rpoD"
                     /gene_synonym="alt; ECK3057; JW3039"
                     /locus_tag="b3067"
                     /label=rpoD
                     /db_xref="EcoGene:EG10896"
                     /db_xref="GeneID:947567"
     CDS             6758..6760
                     /codon_start=1
                     /label=BreR-Bt
                     /translation="M"
     primer_bind     7027..7048
                     /label=internal seq primer
     primer_bind     complement(7347..7374)
                     /label=oMT1309
     CDS             7367..7369
                     /codon_start=1
                     /label=CmeR(NCTC11168)
                     /translation=""
     gene            7367..7369
                     /gene="rpoD"
                     /gene_synonym="alt; ECK3057; JW3039"
                     /locus_tag="b3067"
                     /label=rpoD
                     /db_xref="EcoGene:EG10896"
                     /db_xref="GeneID:947567"
     CDS             7367..7369
                     /codon_start=1
                     /label=BreR-Bt
                     /translation=""
     CDS             7367..7369
                     /label=CmeR(NCTC11168)
     gene            7367..7369
                     /label=rpoD
     CDS             7367..7369
                     /label=BreR-Bt
     primer_bind     7370..7394
                     /label=oMT1188
     misc_feature    7370..7373
                     /label=CAAT
     misc_feature    7380..7385
                     /label=problematic assembly region
     terminator      7386..7446
                     /label=L3S2P21 Terminator
     primer_bind     complement(7425..7465)
                     /label=oMT1272
     misc_feature    7425..7446
                     /label=Feature 67
     primer_bind     7447..7474
                     /label=oMT1259
     primer_bind     complement(7572..7592)
                     /label=pNBU1_seq.REV
     misc_feature    complement(7621..7659)
                     /label=mmD660
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    7660..9402
                     /label=intN1 Construct PCR Product - intN1 Construct P
                     /label=intN1 Construct PCR Product - intN1 Construct PCR 
                     Product 2
                     /note="Geneious type: Inserted sequence"
     misc_feature    7660..8459
                     /label=Piece1
     misc_feature    7660..7689
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
     primer_bind     7660..7688
                     /label=mmD658
     primer_bind     7681..7701
                     /label=oMT113
     misc_feature    7690..8019
                     /label=attN1 Region
     primer_bind     7690..7709
                     /label=intN1 Construct PCR Product (fwd) - pNBU2-PcfxA
                     /label=intN1 Construct PCR Product (fwd) - 
                     pNBU2-PcfxA-NanoLuc PCR Product
     misc_feature    7740..7755
                     /label=DR1
     misc_feature    7773..7787
                     /label=IR1
     misc_feature    7797..7811
                     /label=IR2
     misc_feature    7817..7830
                     /label=CC Region
     misc_feature    7829
                     /label=G-->C
     misc_feature    7829
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     misc_feature    7830
                     /label='-1'
     misc_feature    7831
                     /label='+1'
     misc_feature    7848..7863
                     /label=DR2
     primer_bind     complement(7886..7908)
                     /label=oMT114
     misc_feature    7972..7988
                     /label=DR3
     CDS             8035..8459
                     /codon_start=1
                     /transl_table=11
                     /gene="intN1"
                     /product="IntN1"
                     /label=intN1
                     /label=intN1 CDS
                     /note="NBU1 integrase"
                     /db_xref="GI:8308020"
                     /protein_id="AAF74437.1"
                     /translation="MKVTFIIKKAAKRYDTESMATIYVRFRNGRQLDSVAPTQLAINPN
                     LWDDKDECVKTKAVCNEEMRTHINEEIRQLKTYIEKVYQQEKEAIDKEWLKTTLDKFYH
                     PEKYFLPEEVVIKPTIGELFDEFLNKHPLSEVRKKNF"
     gene            8035..8459
                     /gene="intN1"
                     /label=intN1
                     /label=intN1 gene
     primer_bind     complement(8251..8278)
                     /label=oMT157
     primer_bind     8251..8278
                     /label=oMT156
     misc_feature    complement(8429..8459)
                     /label=mmD659
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    8430..9402
                     /label=Piece2
     CDS             8430..9372
                     /codon_start=1
                     /transl_table=11
                     /gene="intN1"
                     /product="IntN1"
                     /label=intN1
                     /label=intN1 CDS
                     /note="NBU1 integrase"
                     /db_xref="GI:8308020"
                     /protein_id="AAF74437.1"
                     /translation="MVGSTKEKFPGCQKSLTAL*TICKGYKERTKGLYP*CGFGNT*HA
                     SGYVGFLSERIPVL*TLPEHL*SHSRKEDTTAQKQKHADRLFFKNTHILPVVLR*QTHH
                     KQTFRQVSDRGVYIWYTLLYNTRRKGQDF*CRPFCHPTTGNTEGYIHISDTDRMQGERP
                     VPNDQTKCGQ*SHRIYSQENQRGESGYGTCST*RQSERNP*TLQRI*GKTVAVHIRAKV
                     Q*CHKKDIQISWS*PHRNNLRPVDAQRNQTTYL*SGKQPSGKTYVYRQYL*KSERPEPC
                     FRTVGTQGGKQSFQTIQGY*RRNEERPCKTTGL"
     gene            8430..9372
                     /gene="intN1"
                     /label=intN1
                     /label=intN1 gene
     misc_feature    8430..8459
                     /label=Piece2
     misc_feature    8430..8459
                     /label=Ligation
     primer_bind     8430..8449
                     /label=intN1 Construct PCR Product 2 (fwd)
     misc_feature    complement(8435..8459)
                     /label=intN1 Construct PCR Product (rev)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(9348..9374)
                     /label=intN1 Construct PCR Product 2 (rev) - pNBU2-Pcf
                     /label=intN1 Construct PCR Product 2 (rev) - 
                     pNBU2-PcfxA-NanoLuc PCR Product
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    9364
                     /label=T
                     /note="Geneious type: Editing History Replacement"
     misc_feature    complement(9366..9402)
                     /label=mmD661
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    9366
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    9373..9402
                     /label=HOMOLOGY
     misc_feature    9375..9402
                     /label=CTTGTAGTTCGTTTAATTCGCTTTTCGTTTTTACGAATTTAACGAAT
                     /label=CTTGTAGTTCGTTTAATTCGCTTTTCGTTTTTACGAATTTAACGAATTGA 
                     ATATCATTGTTTTACGATTGATTTTCATTATATATTTGCGCCACTATCCTAATCGGGG 
                     TAGTGGCTTTTCGTTTATAATACTGCCGAAACGGGAA
                     /note="Geneious type: Editing History Replacement"
ORIGIN
        1 agagtagcga tattaaaaga atccgatgag aaaagactaa tatttatcta tccattcagt
       61 ttgatttttc aggactttac atcgtcctga aagtatttgt tggtaccggt accgaggacg
      121 cgtaaacatt tacagttgca tgtggcctat tgtttttagc cgttaaatat tttataacta
      181 ttaaatagcg atacaaattg ttcgaaacta atattgttta tatcatatat tctcgcatgt
      241 tttaaagctt tattaaattg attttttgta aacagttttt cgtactcttt gttaacccat
      301 ttcattacaa aagtttcata tttttttctc tctttaaatg ccatttttgc tggctttctt
      361 tttaatacaa ttaatgtgct atccacttta ggttttggat ggaaataata cctaggaatt
      421 tttgctaata tagaaatatc tacctctgcc attaacagca atgctagtga tctgtttgta
      481 tctaataaca ttttagcaaa accatattcc actattaaat aacttattgt ggctgaactt
      541 tcaaaaacaa tttttcgaat tatatttgtg cttatgttgt aaggtatgct gccaaatatt
      601 ttatatggat tgtggctagg aaatgtaaat ttcagtatat catcatttac tatttgatag
      661 ttaggataat ttaagagctt attacgagtt acctcacata atttagaatc aatttctatc
      721 gccgttacaa aattacatct ctttaccaat ccagcagtaa aatgaccttt ccctgcacct
      781 atttcaaaga tgttatcttt ttcatctaaa cttatgcaat tcattatttt ttctatgtga
      841 tattttgaag taataaaatt ttgactatct tttatattta ctttgttcat tataacctct
      901 ccttaattta ttgcatctct tttcgaatat ttatgttttt tgagaaaaga acgtactcat
      961 ggttcatccc gatatgcgta tcggtctgta tatcagcaac tttctatgtg tttcaactac
     1021 aatagtcatc tattctcatc tttctgagtc caccccctgc aaagcccctc tttacgacat
     1081 aaaaattcgg tcggaaaagg tatgcaaaag atgtttctct ctttaagaga aactcttcgg
     1141 gatgcaaaaa tatgaaaata actccaattc accaaattat atagcgactt ttttacaaaa
     1201 tgctaaaatt tgttgatttc cgtcaagcaa ttgttgagca aaaatgtctt ttacgataaa
     1261 atgatacctc aatatcaact gtttagcaaa acgatatttc tcttaaagag agaaacacct
     1321 ttttgttcac caatccccga cttttaatcc cgcggccatg attgaaaaag gaagagtatg
     1381 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1441 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     1501 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     1561 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     1621 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     1681 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     1741 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     1801 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     1861 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     1921 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     1981 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2041 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtcacgc
     2101 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2161 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     2221 ctgattaagc attggtaact gtcagaccaa gtttactcat aacgcgtcaa ttcgaggggg
     2281 atcaattccg tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc
     2341 ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg
     2401 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct
     2461 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
     2521 ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata
     2581 atgaccccga agcagggtta tgcagcggaa aacggaattg atccggccac gatgcgtccg
     2641 gcgtagagga tctgaagatc agcagttcaa cctgttgata gtacgtacta agctctcatg
     2701 tttcacgtac taagctctca tgtttaacgt actaagctct catgtttaac gaactaaacc
     2761 ctcatggcta acgtactaag ctctcatggc taacgtacta agctctcatg tttcacgtac
     2821 taagctctca tgtttgaaca ataaaattaa tataaatcag caacttaaat agcctctaag
     2881 gttttaagtt ttataagaaa aaaaagaata tataaggctt ttaaagcttt taaggtttaa
     2941 cggttgtgga caacaagcca gggatgtaac gcactgagaa gcccttagag cctctcaaag
     3001 caattttgag tgacacagga acacttaacg gctgacatgg gaattcccct ccaccgcggt
     3061 ggattaatgc ggctgcaatt tttttgggcg gggccgccca aaaaaatcct agcaccctgc
     3121 agggtctccc gctggagttt tgcacccgct ttccaagaga agaaagcctt gttaaattga
     3181 cttagtgtaa aagcgcagta ctgcttgacc ataagaacaa aaaaatctct atcactgata
     3241 gggataaagt ttggaagata aagctaaaag ttcttatctt tgcagtctcc ctatcagtga
     3301 tagagacgca caaagataat agaacttaag gttttagagc tagaaatagc aagttaaaat
     3361 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttggatcca
     3421 aaaaaaaaaa acaccctaac gggtgttttt ttttttttgg tctgccgcgg ccgctacgtt
     3481 ttgcacccgc tttccaagag aagaaagcct tgttaaattg acttagtgta aaagcgcagt
     3541 actgcttgac cataagaaca aaaaaatctc tatcactgat agggataaag tttggaagat
     3601 aaagctaaaa gttcttatct ttgcagtctc cctatcagtg atagagacgc acaaagataa
     3661 ttaagttaag ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
     3721 cttgaaaaag tggcaccgag tcggtgcttt ttttggatcc ctcggtacca aattccagaa
     3781 aagagacgct gaaaagcgtc ttttttcgtt ttggtccgcg gccgcaggtt gctgtcatag
     3841 gccggttgtc cggtagtccg gatgatttaa ggaagatttc tcttcttttt tcactttctc
     3901 cgaacaacct ccacttaagt tctattatct ttgcagtcaa agatacttat gttagaagtt
     3961 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     4021 accgagtcgg tgcttttttt ggatccaacg catgagaaag cccccggaag atcaccttcc
     4081 gggggctttt ttattgcgcg cggccgcgct ttgctgtcat aggccggttg tccggtagtc
     4141 cggatgattt aaggaagatt tctcttcttt tttcactttc tccgaacaac ctccacttaa
     4201 gttctattat ctttgcagtc aaagataatt aagttaaggg ttttagagct agaaatagca
     4261 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
     4321 ttggatccct cggtaccaaa ttccagaaaa gagacgctga aaagcgtctt ttttcgtttt
     4381 ggtccgcggc cgccgcttta caaagaaaat tcgacaaact gttatttttc tatctattta
     4441 aatgtacacc cgtttgttta ctttacttta gttatgtacc tttgtcggca atgtacaccc
     4501 gaaagtttac tttcaaagat acttatgtta gaagttttag agctagaaat agcaagttaa
     4561 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttggat
     4621 ccaacgcatg agaaagcccc cggaagatca ccttccgggg gcttttttat tgcgcgcggc
     4681 cgctgtctta caaagaaaat tcgacaaact gttatttttc tatctattta aatgtacacc
     4741 cgtttgttta ctttacttta gttatgtacc tttgtcggca atgtacaccc gaaagtttac
     4801 tttcaaagat aatagaactt aaggttttag agctagaaat agcaagttaa aataaggcta
     4861 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttggat ccaaaaaaaa
     4921 aaaacaccct aacgggtgtt tttttttttt tggtctgccg cggccgcaat gggagtgctg
     4981 tcataggccg gttgtccggt agtccggatg atttaaggaa gatttctctt cttttttcac
     5041 tttctccgaa caacctccat tctaacataa gtatctttgc agtagctgtc accggatgtg
     5101 ctttccggtc tgatgagtcc gtgaggacga aacagcctct acaaataatt ttgtttaacg
     5161 cattttaaaa taaaataaat tatttattta attaaacgaa tatgtaatga actgggatcc
     5221 ctcggtacca aattttcgaa aaaagacgct gaaaagcgtc ttttttcgtt ttggtccgcg
     5281 gccgccggcc gctacgtgct gtcataggcc ggttgtccgg tagtccggat gatttaagga
     5341 agatttctct tcttttttca ctttctccga acaacctcca cttaagttct attatctttg
     5401 cagtagctgt caccggatgt gctttccggt ctgatgagtc cgtgaggacg aaacagcctc
     5461 tacaaataat tttgtttaac gcattttaaa ataaaataaa ttatttattt aattaaacga
     5521 atatgtaatg aactgggatc cctcggtacc aaattttcga aaaaagacgc tgaaaagcgt
     5581 cttttttcgt tttggtccgc ggccgccggc cgcaggttgc tgtcataggc cggttgtccg
     5641 gtagtccgga tgatttaagg aagatttctc ttcttttttc actttctccg aacaacctcc
     5701 accttaactt aattatcttt gcagtagctg tcaccggatg tgctttccgg tctgatgagt
     5761 ccgtgaggac gaaacagcct ctacaaataa ttttgtttaa cgcattttaa aataaaataa
     5821 attatttatt taattaaacg aatatggttt ttactctgga agattttgtt ggcgattggc
     5881 gtcagaccgc gggttataat ttggatcaag tcctggaaca gggtggcgta agctctctgt
     5941 tccagaacct gggtgtgagc gtgacgccga ttcagcgcat cgttctgtcc ggcgagaacg
     6001 gtctgaaaat tgatattcat gtgatcatcc cgtacgaagg cctgagcggt gaccaaatgg
     6061 gtcaaatcga gaaaatcttt aaagtcgtct acccagttga cgatcaccac ttcaaggtta
     6121 tcttgcatta cggtacgctg gtgattgatg gtgtgacccc gaatatgatt gactatttcg
     6181 gccgtccgta tgaaggcatt gccgtttttg acggtaaaaa gatcaccgtc accggtaccc
     6241 tgtggaatgg caataagatt attgacgagc gtctgattaa cccggacggc agcctgctgt
     6301 tccgcgtgac catcaacggt gtcacgggtt ggcgtctgtg cgagcgcatc ctggcataat
     6361 gaactgggat ccctcggtac caaattttcg aaaaaagacg ctgaaaagcg tcttttttcg
     6421 ttttggtccg cggccgccgg ccgcaatgtg atctggaaga agcaatgaaa gctgctgtta
     6481 agtctccgaa tcaggtattg ttcctgacag gtgtattccc atccggtaaa cgcggatact
     6541 ttgcagttga tctgactcag gaataaatta taaattaagg taagaagatt gtaggataag
     6601 ctaatgaaat agaaaaagga tgccgtcaca caacttgtcg gcattctttt ttgttttatt
     6661 agttgaaaat atagtgaaaa agttgcctaa atatgtatgt taacaaatta tttgtcgtaa
     6721 ctttgcactc caaatctgtt tttaacatat ggcactaatg cagaaaaaat tgactcgttc
     6781 tcagcagaaa cacttggaca tcatcaacgc tgctaaagaa gaattcatcg aattcggttt
     6841 cttggctgct aacatggacc gtatcacttc ttctgctgaa gtatctaaac gtactttgta
     6901 ccgtcacttc gaatctaaag aagtattgtt cgaatctgta ttgactatca tcaacgactc
     6961 tgtaaacgaa tctatctctt accacttcga cccgaacaaa tctactgaag aacagttgac
     7021 tgaaatcgct tacaaagaaa tcgacgtatt gtacaaaact tacggtatcg ctttggctcg
     7081 tactatcgta atggaattct tgcgtcagcc ggaaatggct aaaactttga tccagaacat
     7141 ctactctatc cgtgctatca ctcagtggtt ccgttctgct atcgaagcta aacgtttgaa
     7201 agacgctgac ccgaaattga tgactgacgt atacgtatct ttgttccagg gtttgttctt
     7261 ctggccgcag gtaatgcact tggacttgga accgcacggt gaagaattgt ctcagaaaat
     7321 cgaaactttg actactatct tcttgcagtc ttacggtgta gctgaataac aatgaatggg
     7381 ctttcctcgg taccaaattc cagaaaagag gcctcccgaa aggggggcct tttttcgttt
     7441 tggtccctaa ttgcctatct tccagtgatg gaacagcatt tgtgcattgg ctgcaacaat
     7501 cagccttact tgtgcctgtt ctatttccga accgaccgct tgtatgaatc catcaaaatt
     7561 cgttttctct atgttggatt ccttgttgct catattgtga tgataatttc tacaaatata
     7621 gtcattggta actatctatg aaactgtttg atacttttac tcatcggatt cttttaatat
     7681 cgctactctc tacgttcaac caaaagaaat aatgacttac tgctatattt tttgcacgtg
     7741 tggggaaaat gtggggaaaa ttcaagcaaa agaaaaagct aagtattgaa ctatcaaata
     7801 cttagctttc tttcttgtac ccagaccccg catttgaaat aattaaagtg gggaaaatgt
     7861 gggtaaaaag aaaaatgcgg aaaaacgcca caattacact gtatttcaat atgttataat
     7921 cctattaaat tttaatccaa gtttaatcga attgcaaaat atttagcaga tgtggggaaa
     7981 atgctgggga aaatatttat atttgcagca gagtaaaatt caacctgaaa gtctatgaaa
     8041 gtaaccttta tcattaaaaa agcagccaaa cgatatgata cagaatccat ggctacaatc
     8101 tatgtccgtt ttagaaacgg aaggcagtta gactccgttg ctcctactca gttagccatc
     8161 aatcccaatc tatgggatga taaagacgaa tgtgtaaaaa cgaaagctgt ctgcaatgaa
     8221 gaaatgcgta cccatataaa tgaagagata cgccagttga aaacctatat cgagaaggta
     8281 tatcaacaag aaaaggaagc aatagacaaa gaatggctaa aaacaacact tgataaattt
     8341 taccatcctg aaaaatattt tttgccggag gaagtggtta tcaagcctac cattggagaa
     8401 ctattcgatg aatttctaaa caagcaccct ttgtcggaag tacgaaagaa aaatttccgg
     8461 gttgtcaaaa gagccttact gcgttatgaa ctatatgtaa gggctacaaa gagaggacaa
     8521 aagggcttta tccttgatgt ggatttggta acacctgaca cgcttcggga tatgtgggat
     8581 ttctttcaga acgaatacca gtattatgaa ctttacccga gcatttatga agccattccc
     8641 gaaaagagga caccacagcc cagaagcaaa aacacgctga tagactgttt ttcaagaata
     8701 cgcacattct tcctgtggtg cttcgataac aaacgcacca caaacagacc tttcgacaag
     8761 tttccgatag aggagtgtac atatggtaca ccttattata taacactcga agaaagggac
     8821 aggattttta atgcagacct ttctgccacc ccacaactgg caatacagag ggatatattc
     8881 atatttcaga cactgatagg atgcagggtg agcgacctgt accgaatgac caaactaaat
     8941 gtggtcaatg aagccataga atatattccc aagaaaacca aagaggggaa tccggttacg
     9001 gtacgtgttc cacttaacga caaagcgaaa gaaatccttg aacgctacaa agaatatgag
     9061 ggaaaactgt tgccgttcat atccgagcaa aagtacaatg atgccataaa aaagatattc
     9121 aaattagctg gagttgaccg catcgtaaca atcttagacc cgttgacgca caacgaaatc
     9181 aaacgaccta tttatgaagt ggcaagcagc catctggcaa gacgtacgtt tatcggcaat
     9241 atctataaaa aagtgaaaga cccgaacctt gtttccgcac tgtcgggaca caaggaggga
     9301 agcaaagctt tcagacgata cagggatatt gacgaagaaa tgaagaaaga ccttgtaaaa
     9361 ctactggact gataaaagta tcaaacagtt tcatagatag tt
//
