LOCUS       Exported                7191 bp ds-DNA     circular SYN 03-NOV-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 20, 2020 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..30
                     /label=mmD661
     misc_feature    1
                     /label=origin
     misc_recomb     118..2262
                     /label=NluI fragment from pKNOCK
                     /note="NluI fragment from pKNOCK"
     CDS             complement(156..890)
                     /label=ermG
                     /note="ermG"
     CDS             1378..2239
                     /label=beta-lactamase from pBR322
                     /note="beta-lactamase from pBR322"
     primer_bind     1837..1856
                     /label=oMT203
     primer_bind     2089..2106
                     /label=oMT1184
     primer_bind     complement(2089..2106)
                     /label=oMT1189
     misc_feature    2097
                     /label=t2096a
     misc_recomb     2277..3062
                     /label=RP4 oriT/RSK oriV
                     /note="RP4 oriT/RSK oriV"
     primer_bind     complement(2340..2359)
                     /label=oMT204
     primer_bind     2968..2987
                     /label=pNBU1_seq.FOR
     misc_feature    3062
                     /label=3062 mutation from ""g"" to ""t""
                     /note="Mutated in pMT1fix2, but will not fix, since it's at
                     the backbone of the vector and is not CDS or a region with 
                     important UTR."
     misc_feature    3063..3122
                     /label=pNBU2-Lac-dCas9-NL3 PCR Product - pNBU2-Lac-dCas9-
                     /note="Geneious type: Inserted sequence"
     terminator      3078..3107
                     /label=tL17 terminator
                     /label=tL17
     primer_bind     complement(3105..3139)
                     /label=oMT1452
     primer_bind     3122..3148
                     /label=oMT1449
     primer_bind     3123..3159
                     /label=oMT1293
     misc_feature    3123..3129
                     /label=Scar
     misc_feature    3130..3133
                     /label=CGCT
     promoter        3134..3176
                     /label=cfxA_promoter
     misc_feature    3177..3185
                     /label=AANGTANAC
     misc_feature    3186..3191
                     /label=N6
     misc_feature    3189..3192
                     /label=-33
     misc_feature    3192..3200
                     /label=GTNTACNTT
     -35_signal      3193..3198
                     /label=-35
     promoter        3201..3212
                     /label=PcfxA
     misc_feature    3213..3220
                     /label=-7
     misc_feature    3222..3225
                     /label=4bp-2
     primer_bind     complement(3223..3249)
                     /label=oMT916
     misc_feature    3225
                     /label=+1
     misc_feature    3226..3234
                     /label=AANGTANAC
     misc_feature    3235..3240
                     /label=N6
     misc_feature    3241..3249
                     /label=GTNTACNTT
     -35_signal      3242..3247
                     /label=-35
     misc_feature    3250..3269
                     /label=sgRNA-10
     misc_feature    3270..3311
                     /label=chimera tracrRNA
     primer_bind     3270..3303
                     /label=oMT915
     misc_feature    3316..3352
                     /label=S. pyogenes terminator
     primer_bind     complement(3333..3430)
                     /label=oMT1443
     misc_feature    3353..3399
                     /label=ECK125109870
     primer_bind     3392..3536
                     /label=oMT1440
     misc_feature    3403..3410
                     /label=Restriction Sites
     primer_bind     3410..3536
                     /label=oMT914
     primer_bind     3411..3536
                     /label=oMT1444
     misc_feature    3411..3486
                     /label=PBF2884
     primer_bind     3411..3430
                     /label=oMT1006
     misc_feature    3453..3457
                     /label=-60
     primer_bind     complement(3461..3491)
                     /label=oMT1351
     primer_bind     complement(3461..3489)
                     /label=oMT1116
     misc_feature    3464..3468
                     /label=-50
     primer_bind     3470..3494
                     /label=oMT1114
     primer_bind     complement(3471..3494)
                     /label=oMT1064
     misc_feature    3483..3486
                     /label=-33
     misc_feature    3492..3493
                     /label=PAM
     misc_feature    3493..3494
                     /label=CATA
     misc_feature    3495..3514
                     /label=sgRNA-10
     misc_feature    3519..3593
                     /label=RiboJ00
     terminator      3594..3636
                     /label=rpiL*
                     /note="rpiL* termninator"
     CDS             3637..4152
                     /codon_start=1
                     /label=Luciferase Reporter
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     3637..3661
                     /label=oMT41
     primer_bind     complement(3648..3667)
                     /label=oMT386
     primer_bind     3925..3944
                     /label=oMT399
     misc_feature    4124..4152
                     /label=problematic assembly region
     primer_bind     complement(4131..4156)
                     /label=oMT1343
     primer_bind     complement(4134..4152)
                     /label=oMT1307
     primer_bind     4153..4263
                     /label=oMT1348
     primer_bind     4153..4263
                     /label=oMT1344
     primer_bind     4153..4171
                     /label=oMT1431
     misc_feature    4153..4156
                     /label=TGAA
     misc_feature    4157..5158
                     /label=Amp up this region
     misc_feature    4160..4230
                     /label=L3S2P56
     misc_feature    4223..4224
                     /label=Restriction Sites
     promoter        4238..4520
                     /label=BT1311_promoter
     primer_bind     4416..4435
                     /label=oMT1135
     -35_signal      4481..4484
                     /label=-33
     primer_bind     4502..4521
                     /label=oMT100
     -10_signal      4507..4514
                     /label=-7
     RBS             4521..4546
                     /label=BT1311_RBS
     CDS             4547..5158
                     /codon_start=1
                     /label=VFA0359-Bt
                     /translation="MQKKLTRSQQKHLDIINAAKEEFIEFGFLAANMDRITSSAEVSKR
                     TLYRHFESKEVLFESVLTIINDSVNESISYHFDPNKSTEEQLTEIAYKEIDVLYKTYGI
                     ALARTIVMEFLRQPEMAKTLIQNIYSIRAITQWFRSAIEAKRLKDADPKLMTDVYVSLF
                     QGLFFWPQVMHLDLEPHGEELSQKIETLTTIFLQSYGVAE"
     CDS             4547..4549
                     /codon_start=1
                     /label=BreR-Bt
                     /translation="M"
     gene            4547..4549
                     /gene="rpoD"
                     /gene_synonym="alt; ECK3057; JW3039"
                     /locus_tag="b3067"
                     /label=rpoD
                     /db_xref="EcoGene:EG10896"
                     /db_xref="GeneID:947567"
     CDS             4547..4549
                     /codon_start=1
                     /label=CmeR(NCTC11168)
                     /translation="M"
     primer_bind     4816..4837
                     /label=internal seq primer
     primer_bind     4816..4837
                     /label=oMT379
     primer_bind     complement(5129..5153)
                     /label=oMT677
     primer_bind     complement(5131..5158)
                     /label=oMT1450
     primer_bind     complement(5136..5163)
                     /label=oMT1309
     primer_bind     5139..5179
                     /label=oMT1451
     CDS             5156..5158
                     /label=BreR-Bt
     gene            5156..5158
                     /label=rpoD
     CDS             5156..5158
                     /label=CmeR(NCTC11168)
     CDS             5156..5158
                     /codon_start=1
                     /label=BreR-Bt
                     /translation=""
     gene            5156..5158
                     /gene="rpoD"
                     /gene_synonym="alt; ECK3057; JW3039"
                     /locus_tag="b3067"
                     /label=rpoD
                     /db_xref="EcoGene:EG10896"
                     /db_xref="GeneID:947567"
     CDS             5156..5158
                     /codon_start=1
                     /label=CmeR(NCTC11168)
                     /translation=""
     misc_feature    5159..5168
                     /label=Amp up this region
     misc_feature    5159..5162
                     /label=CAAT
     primer_bind     5169..5183
                     /label=oMT676
     misc_feature    5169..5174
                     /label=problematic assembly region
     terminator      5175..5235
                     /label=L3S2P21 Terminator
     primer_bind     complement(5214..5254)
                     /label=oMT1272
     misc_feature    5214..5235
                     /label=Feature 67
     primer_bind     5236..5263
                     /label=oMT1259
     primer_bind     complement(5361..5381)
                     /label=pNBU1_seq.REV
     misc_feature    complement(5410..5448)
                     /label=mmD660
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    5449..7191
                     /label=intN1 Construct PCR Product - intN1 Construct P
                     /label=intN1 Construct PCR Product - intN1 Construct PCR 
                     Product 2
                     /note="Geneious type: Inserted sequence"
     misc_feature    5449..6248
                     /label=Piece1
     misc_feature    5449..5478
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
     primer_bind     5449..5477
                     /label=mmD658
     primer_bind     5470..5490
                     /label=oMT113
     misc_feature    5479..5808
                     /label=attN1 Region
     primer_bind     5479..5498
                     /label=intN1 Construct PCR Product (fwd) - pNBU2-PcfxA
                     /label=intN1 Construct PCR Product (fwd) - 
                     pNBU2-PcfxA-NanoLuc PCR Product
     misc_feature    5529..5544
                     /label=DR1
     misc_feature    5562..5576
                     /label=IR1
     misc_feature    5586..5600
                     /label=IR2
     misc_feature    5606..5619
                     /label=CC Region
     misc_feature    5618
                     /label=G-->C
     misc_feature    5618
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     misc_feature    5619
                     /label='-1'
     misc_feature    5620
                     /label='+1'
     misc_feature    5637..5652
                     /label=DR2
     primer_bind     complement(5675..5697)
                     /label=oMT114
     misc_feature    5761..5777
                     /label=DR3
     CDS             5824..6248
                     /codon_start=1
                     /transl_table=11
                     /gene="intN1"
                     /product="IntN1"
                     /label=intN1
                     /label=intN1 CDS
                     /note="NBU1 integrase"
                     /db_xref="GI:8308020"
                     /protein_id="AAF74437.1"
                     /translation="MKVTFIIKKAAKRYDTESMATIYVRFRNGRQLDSVAPTQLAINPN
                     LWDDKDECVKTKAVCNEEMRTHINEEIRQLKTYIEKVYQQEKEAIDKEWLKTTLDKFYH
                     PEKYFLPEEVVIKPTIGELFDEFLNKHPLSEVRKKNF"
     gene            5824..6248
                     /gene="intN1"
                     /label=intN1
                     /label=intN1 gene
     primer_bind     6040..6067
                     /label=oMT156
     primer_bind     complement(6040..6067)
                     /label=oMT157
     misc_feature    complement(6218..6248)
                     /label=mmD659
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    6219..7191
                     /label=Piece2
     CDS             6219..7161
                     /codon_start=1
                     /transl_table=11
                     /gene="intN1"
                     /product="IntN1"
                     /label=intN1
                     /label=intN1 CDS
                     /note="NBU1 integrase"
                     /db_xref="GI:8308020"
                     /protein_id="AAF74437.1"
                     /translation="MVGSTKEKFPGCQKSLTAL*TICKGYKERTKGLYP*CGFGNT*HA
                     SGYVGFLSERIPVL*TLPEHL*SHSRKEDTTAQKQKHADRLFFKNTHILPVVLR*QTHH
                     KQTFRQVSDRGVYIWYTLLYNTRRKGQDF*CRPFCHPTTGNTEGYIHISDTDRMQGERP
                     VPNDQTKCGQ*SHRIYSQENQRGESGYGTCST*RQSERNP*TLQRI*GKTVAVHIRAKV
                     Q*CHKKDIQISWS*PHRNNLRPVDAQRNQTTYL*SGKQPSGKTYVYRQYL*KSERPEPC
                     FRTVGTQGGKQSFQTIQGY*RRNEERPCKTTGL"
     gene            6219..7161
                     /gene="intN1"
                     /label=intN1
                     /label=intN1 gene
     misc_feature    6219..6248
                     /label=Piece2
     misc_feature    6219..6248
                     /label=Ligation
     primer_bind     6219..6238
                     /label=intN1 Construct PCR Product 2 (fwd)
     misc_feature    complement(6224..6248)
                     /label=intN1 Construct PCR Product (rev)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(7137..7163)
                     /label=intN1 Construct PCR Product 2 (rev) - pNBU2-Pcf
                     /label=intN1 Construct PCR Product 2 (rev) - 
                     pNBU2-PcfxA-NanoLuc PCR Product
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    7153
                     /label=T
                     /note="Geneious type: Editing History Replacement"
     misc_feature    complement(7155..7191)
                     /label=mmD661
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    7155
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    7162..7191
                     /label=HOMOLOGY
     misc_feature    7164..7191
                     /label=CTTGTAGTTCGTTTAATTCGCTTTTCGTTTTTACGAATTTAACGAAT
                     /label=CTTGTAGTTCGTTTAATTCGCTTTTCGTTTTTACGAATTTAACGAATTGA 
                     ATATCATTGTTTTACGATTGATTTTCATTATATATTTGCGCCACTATCCTAATCGGGG 
                     TAGTGGCTTTTCGTTTATAATACTGCCGAAACGGGAA
                     /note="Geneious type: Editing History Replacement"
ORIGIN
        1 agagtagcga tattaaaaga atccgatgag aaaagactaa tatttatcta tccattcagt
       61 ttgatttttc aggactttac atcgtcctga aagtatttgt tggtaccggt accgaggacg
      121 cgtaaacatt tacagttgca tgtggcctat tgtttttagc cgttaaatat tttataacta
      181 ttaaatagcg atacaaattg ttcgaaacta atattgttta tatcatatat tctcgcatgt
      241 tttaaagctt tattaaattg attttttgta aacagttttt cgtactcttt gttaacccat
      301 ttcattacaa aagtttcata tttttttctc tctttaaatg ccatttttgc tggctttctt
      361 tttaatacaa ttaatgtgct atccacttta ggttttggat ggaaataata cctaggaatt
      421 tttgctaata tagaaatatc tacctctgcc attaacagca atgctagtga tctgtttgta
      481 tctaataaca ttttagcaaa accatattcc actattaaat aacttattgt ggctgaactt
      541 tcaaaaacaa tttttcgaat tatatttgtg cttatgttgt aaggtatgct gccaaatatt
      601 ttatatggat tgtggctagg aaatgtaaat ttcagtatat catcatttac tatttgatag
      661 ttaggataat ttaagagctt attacgagtt acctcacata atttagaatc aatttctatc
      721 gccgttacaa aattacatct ctttaccaat ccagcagtaa aatgaccttt ccctgcacct
      781 atttcaaaga tgttatcttt ttcatctaaa cttatgcaat tcattatttt ttctatgtga
      841 tattttgaag taataaaatt ttgactatct tttatattta ctttgttcat tataacctct
      901 ccttaattta ttgcatctct tttcgaatat ttatgttttt tgagaaaaga acgtactcat
      961 ggttcatccc gatatgcgta tcggtctgta tatcagcaac tttctatgtg tttcaactac
     1021 aatagtcatc tattctcatc tttctgagtc caccccctgc aaagcccctc tttacgacat
     1081 aaaaattcgg tcggaaaagg tatgcaaaag atgtttctct ctttaagaga aactcttcgg
     1141 gatgcaaaaa tatgaaaata actccaattc accaaattat atagcgactt ttttacaaaa
     1201 tgctaaaatt tgttgatttc cgtcaagcaa ttgttgagca aaaatgtctt ttacgataaa
     1261 atgatacctc aatatcaact gtttagcaaa acgatatttc tcttaaagag agaaacacct
     1321 ttttgttcac caatccccga cttttaatcc cgcggccatg attgaaaaag gaagagtatg
     1381 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1441 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     1501 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     1561 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     1621 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     1681 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     1741 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     1801 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     1861 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     1921 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     1981 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2041 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtcacgc
     2101 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2161 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     2221 ctgattaagc attggtaact gtcagaccaa gtttactcat aacgcgtcaa ttcgaggggg
     2281 atcaattccg tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc
     2341 ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg
     2401 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct
     2461 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
     2521 ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata
     2581 atgaccccga agcagggtta tgcagcggaa aacggaattg atccggccac gatgcgtccg
     2641 gcgtagagga tctgaagatc agcagttcaa cctgttgata gtacgtacta agctctcatg
     2701 tttcacgtac taagctctca tgtttaacgt actaagctct catgtttaac gaactaaacc
     2761 ctcatggcta acgtactaag ctctcatggc taacgtacta agctctcatg tttcacgtac
     2821 taagctctca tgtttgaaca ataaaattaa tataaatcag caacttaaat agcctctaag
     2881 gttttaagtt ttataagaaa aaaaagaata tataaggctt ttaaagcttt taaggtttaa
     2941 cggttgtgga caacaagcca gggatgtaac gcactgagaa gcccttagag cctctcaaag
     3001 caattttgag tgacacagga acacttaacg gctgacatgg gaattcccct ccaccgcggt
     3061 ggattaatgc ggctgcaatt tttttgggcg gggccgccca aaaaaatcct agcaccctgc
     3121 agggtctccc gctttacaaa gaaaattcga caaactgtta tttttctatc tatttaaatg
     3181 tacacccgtt tgtttacttt actttagtta tgtacctttg tcggcaatgt acacccgaaa
     3241 gtttactttc aaagatagaa taagtctttg ttttagagct agaaatagca agttaaaata
     3301 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttccaattat
     3361 tgaacaccct aacgggtgtt tttttgtttc tggtctcccg ccgcggccgc tgctgtcata
     3421 ggccggttgt ccggtagtcc ggatgattta aggaagattt ctcttctttt ttcactttct
     3481 ccgaacaacc tccaaaagac ttattctatc tttgcagtag ctgtcaccgg atgtgctttc
     3541 cggtctgatg agtccgtgag gacgaaacag cctctacaaa taattttgtt taacgcattt
     3601 taaaataaaa taaattattt atttaattaa acgaatatgg tttttactct ggaagatttt
     3661 gttggcgatt ggcgtcagac cgcgggttat aatttggatc aagtcctgga acagggtggc
     3721 gtaagctctc tgttccagaa cctgggtgtg agcgtgacgc cgattcagcg catcgttctg
     3781 tccggcgaga acggtctgaa aattgatatt catgtgatca tcccgtacga aggcctgagc
     3841 ggtgaccaaa tgggtcaaat cgagaaaatc tttaaagtcg tctacccagt tgacgatcac
     3901 cacttcaagg ttatcttgca ttacggtacg ctggtgattg atggtgtgac cccgaatatg
     3961 attgactatt tcggccgtcc gtatgaaggc attgccgttt ttgacggtaa aaagatcacc
     4021 gtcaccggta ccctgtggaa tggcaataag attattgacg agcgtctgat taacccggac
     4081 ggcagcctgc tgttccgcgt gaccatcaac ggtgtcacgg gttggcgtct gtgcgagcgc
     4141 atcctggcat aatgaactgg gatccctcgg taccaaattt tcgaaaaaag acgctgaaaa
     4201 gcgtcttttt tcgttttggt ccgcggccgc cggccgctga tctggaagaa gcaatgaaag
     4261 ctgctgttaa gtctccgaat caggtattgt tcctgacagg tgtattccca tccggtaaac
     4321 gcggatactt tgcagttgat ctgactcagg aataaattat aaattaaggt aagaagattg
     4381 taggataagc taatgaaata gaaaaaggat gccgtcacac aacttgtcgg cattcttttt
     4441 tgttttatta gttgaaaata tagtgaaaaa gttgcctaaa tatgtatgtt aacaaattat
     4501 ttgtcgtaac tttgcactcc aaatctgttt ttaacatatg gcactaatgc agaaaaaatt
     4561 gactcgttct cagcagaaac acttggacat catcaacgct gctaaagaag aattcatcga
     4621 attcggtttc ttggctgcta acatggaccg tatcacttct tctgctgaag tatctaaacg
     4681 tactttgtac cgtcacttcg aatctaaaga agtattgttc gaatctgtat tgactatcat
     4741 caacgactct gtaaacgaat ctatctctta ccacttcgac ccgaacaaat ctactgaaga
     4801 acagttgact gaaatcgctt acaaagaaat cgacgtattg tacaaaactt acggtatcgc
     4861 tttggctcgt actatcgtaa tggaattctt gcgtcagccg gaaatggcta aaactttgat
     4921 ccagaacatc tactctatcc gtgctatcac tcagtggttc cgttctgcta tcgaagctaa
     4981 acgtttgaaa gacgctgacc cgaaattgat gactgacgta tacgtatctt tgttccaggg
     5041 tttgttcttc tggccgcagg taatgcactt ggacttggaa ccgcacggtg aagaattgtc
     5101 tcagaaaatc gaaactttga ctactatctt cttgcagtct tacggtgtag ctgaataaca
     5161 atgaatgggc tttcctcggt accaaattcc agaaaagagg cctcccgaaa ggggggcctt
     5221 ttttcgtttt ggtccctaat tgcctatctt ccagtgatgg aacagcattt gtgcattggc
     5281 tgcaacaatc agccttactt gtgcctgttc tatttccgaa ccgaccgctt gtatgaatcc
     5341 atcaaaattc gttttctcta tgttggattc cttgttgctc atattgtgat gataatttct
     5401 acaaatatag tcattggtaa ctatctatga aactgtttga tacttttact catcggattc
     5461 ttttaatatc gctactctct acgttcaacc aaaagaaata atgacttact gctatatttt
     5521 ttgcacgtgt ggggaaaatg tggggaaaat tcaagcaaaa gaaaaagcta agtattgaac
     5581 tatcaaatac ttagctttct ttcttgtacc cagaccccgc atttgaaata attaaagtgg
     5641 ggaaaatgtg ggtaaaaaga aaaatgcgga aaaacgccac aattacactg tatttcaata
     5701 tgttataatc ctattaaatt ttaatccaag tttaatcgaa ttgcaaaata tttagcagat
     5761 gtggggaaaa tgctggggaa aatatttata tttgcagcag agtaaaattc aacctgaaag
     5821 tctatgaaag taacctttat cattaaaaaa gcagccaaac gatatgatac agaatccatg
     5881 gctacaatct atgtccgttt tagaaacgga aggcagttag actccgttgc tcctactcag
     5941 ttagccatca atcccaatct atgggatgat aaagacgaat gtgtaaaaac gaaagctgtc
     6001 tgcaatgaag aaatgcgtac ccatataaat gaagagatac gccagttgaa aacctatatc
     6061 gagaaggtat atcaacaaga aaaggaagca atagacaaag aatggctaaa aacaacactt
     6121 gataaatttt accatcctga aaaatatttt ttgccggagg aagtggttat caagcctacc
     6181 attggagaac tattcgatga atttctaaac aagcaccctt tgtcggaagt acgaaagaaa
     6241 aatttccggg ttgtcaaaag agccttactg cgttatgaac tatatgtaag ggctacaaag
     6301 agaggacaaa agggctttat ccttgatgtg gatttggtaa cacctgacac gcttcgggat
     6361 atgtgggatt tctttcagaa cgaataccag tattatgaac tttacccgag catttatgaa
     6421 gccattcccg aaaagaggac accacagccc agaagcaaaa acacgctgat agactgtttt
     6481 tcaagaatac gcacattctt cctgtggtgc ttcgataaca aacgcaccac aaacagacct
     6541 ttcgacaagt ttccgataga ggagtgtaca tatggtacac cttattatat aacactcgaa
     6601 gaaagggaca ggatttttaa tgcagacctt tctgccaccc cacaactggc aatacagagg
     6661 gatatattca tatttcagac actgatagga tgcagggtga gcgacctgta ccgaatgacc
     6721 aaactaaatg tggtcaatga agccatagaa tatattccca agaaaaccaa agaggggaat
     6781 ccggttacgg tacgtgttcc acttaacgac aaagcgaaag aaatccttga acgctacaaa
     6841 gaatatgagg gaaaactgtt gccgttcata tccgagcaaa agtacaatga tgccataaaa
     6901 aagatattca aattagctgg agttgaccgc atcgtaacaa tcttagaccc gttgacgcac
     6961 aacgaaatca aacgacctat ttatgaagtg gcaagcagcc atctggcaag acgtacgttt
     7021 atcggcaata tctataaaaa agtgaaagac ccgaaccttg tttccgcact gtcgggacac
     7081 aaggagggaa gcaaagcttt cagacgatac agggatattg acgaagaaat gaagaaagac
     7141 cttgtaaaac tactggactg ataaaagtat caaacagttt catagatagt t
//
