LOCUS       Exported                7205 bp ds-DNA     circular SYN 03-NOV-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7205)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 20, 2020 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7205
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..30
                     /label=mmD661
     misc_feature    1
                     /label=origin
     misc_recomb     118..2262
                     /label=NluI fragment from pKNOCK
                     /note="NluI fragment from pKNOCK"
     CDS             complement(156..890)
                     /label=ermG
                     /note="ermG"
     CDS             1378..2239
                     /label=beta-lactamase from pBR322
                     /note="beta-lactamase from pBR322"
     primer_bind     1837..1856
                     /label=oMT203
     primer_bind     2089..2106
                     /label=oMT1184
     primer_bind     complement(2089..2106)
                     /label=oMT1189
     misc_feature    2097
                     /label=t2096a
     misc_recomb     2277..3062
                     /label=RP4 oriT/RSK oriV
                     /note="RP4 oriT/RSK oriV"
     primer_bind     complement(2340..2359)
                     /label=oMT204
     primer_bind     2968..2987
                     /label=pNBU1_seq.FOR
     misc_feature    3062
                     /label=3062 mutation from ""g"" to ""t""
                     /note="Mutated in pMT1fix2, but will not fix, since it's at
                     the backbone of the vector and is not CDS or a region with 
                     important UTR."
     misc_feature    3063..3122
                     /label=pNBU2-Lac-dCas9-NL3 PCR Product - pNBU2-Lac-dCas9-
                     /note="Geneious type: Inserted sequence"
     terminator      3078..3107
                     /label=tL17 terminator
                     /label=tL17
     primer_bind     3123..3159
                     /label=oMT1293
     misc_feature    3123..3129
                     /label=Scar
     misc_feature    3130..3133
                     /label=CGCT
     promoter        3134..3176
                     /label=cfxA_promoter
     misc_feature    3177..3185
                     /label=AANGTANAC
     misc_feature    3186..3191
                     /label=N6
     misc_feature    3189..3192
                     /label=-33
     misc_feature    3192..3200
                     /label=GTNTACNTT
     -35_signal      3193..3198
                     /label=-35
     promoter        3201..3212
                     /label=PcfxA
     misc_feature    3213..3220
                     /label=-7
     misc_feature    3222..3225
                     /label=4bp-2
     primer_bind     complement(3223..3249)
                     /label=oMT916
     misc_feature    3225
                     /label=+1
     misc_feature    3226..3234
                     /label=AANGTANAC
     misc_feature    3235..3240
                     /label=N6
     misc_feature    3241..3249
                     /label=GTNTACNTT
     -35_signal      3242..3247
                     /label=-35
     misc_feature    3250..3269
                     /label=sgRNA-11
     misc_feature    3270..3311
                     /label=chimera tracrRNA
     primer_bind     3270..3303
                     /label=oMT915
     misc_feature    3316..3352
                     /label=S. pyogenes terminator
     primer_bind     complement(3333..3444)
                     /label=oMT1445
     primer_bind     complement(3333..3409)
                     /label=oMT1432
     misc_feature    3353..3413
                     /label=L3S3P31
     misc_feature    3354..3358
                     /label=Amp up this region
     primer_bind     3398..3550
                     /label=oMT1441
     misc_feature    3405..3408
                     /label=CGCG
     misc_feature    3409
                     /label=mutated to A
     misc_feature    3417..3424
                     /label=Restriction Sites
     primer_bind     3424..3550
                     /label=oMT914
     primer_bind     3425..3550
                     /label=oMT1446
     misc_feature    3425..3500
                     /label=PBF2884
     primer_bind     3425..3444
                     /label=oMT1006
     misc_feature    3467..3471
                     /label=-60
     primer_bind     complement(3475..3505)
                     /label=oMT1351
     primer_bind     complement(3475..3503)
                     /label=oMT1116
     misc_feature    3478..3482
                     /label=-50
     primer_bind     3484..3508
                     /label=oMT1114
     primer_bind     complement(3485..3508)
                     /label=oMT1064
     misc_feature    3497..3500
                     /label=-33
     misc_feature    3506..3507
                     /label=PAM
     misc_feature    3507..3508
                     /label=CATA
     misc_feature    3509..3528
                     /label=sgRNA-11
     misc_feature    3533..3607
                     /label=RiboJ00
     terminator      3608..3650
                     /label=rpiL*
                     /note="rpiL* termninator"
     CDS             3651..4166
                     /codon_start=1
                     /label=Luciferase Reporter
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     3651..3675
                     /label=oMT41
     primer_bind     complement(3662..3681)
                     /label=oMT386
     primer_bind     3939..3958
                     /label=oMT399
     misc_feature    4138..4166
                     /label=problematic assembly region
     primer_bind     complement(4145..4170)
                     /label=oMT1343
     primer_bind     complement(4145..4166)
                     /label=oMT1347
     primer_bind     complement(4148..4166)
                     /label=oMT1349
     primer_bind     complement(4148..4166)
                     /label=oMT1307
     primer_bind     4167..4277
                     /label=oMT1348
     primer_bind     4167..4277
                     /label=oMT1344
     primer_bind     4167..4185
                     /label=oMT1431
     misc_feature    4167..4170
                     /label=TGAA
     misc_feature    4167..4170
                     /label=Amp up this region
     misc_feature    4171..5172
                     /label=Amp up this region
     misc_feature    4174..4244
                     /label=L3S2P56
     misc_feature    4237..4238
                     /label=Restriction Sites
     promoter        4252..4534
                     /label=BT1311_promoter
     primer_bind     4430..4449
                     /label=oMT1135
     -35_signal      4495..4498
                     /label=-33
     primer_bind     4516..4535
                     /label=oMT100
     -10_signal      4521..4528
                     /label=-7
     RBS             4535..4560
                     /label=BT1311_RBS
     CDS             4561..5172
                     /codon_start=1
                     /label=VFA0359-Bt
                     /translation="MQKKLTRSQQKHLDIINAAKEEFIEFGFLAANMDRITSSAEVSKR
                     TLYRHFESKEVLFESVLTIINDSVNESISYHFDPNKSTEEQLTEIAYKEIDVLYKTYGI
                     ALARTIVMEFLRQPEMAKTLIQNIYSIRAITQWFRSAIEAKRLKDADPKLMTDVYVSLF
                     QGLFFWPQVMHLDLEPHGEELSQKIETLTTIFLQSYGVAE"
     CDS             4561..4563
                     /codon_start=1
                     /label=CmeR(NCTC11168)
                     /translation="M"
     gene            4561..4563
                     /gene="rpoD"
                     /gene_synonym="alt; ECK3057; JW3039"
                     /locus_tag="b3067"
                     /label=rpoD
                     /db_xref="EcoGene:EG10896"
                     /db_xref="GeneID:947567"
     CDS             4561..4563
                     /codon_start=1
                     /label=BreR-Bt
                     /translation="M"
     primer_bind     4830..4851
                     /label=internal seq primer
     primer_bind     4830..4851
                     /label=oMT379
     primer_bind     complement(5143..5167)
                     /label=oMT677
     primer_bind     complement(5150..5177)
                     /label=oMT1309
     CDS             5170..5172
                     /codon_start=1
                     /label=CmeR(NCTC11168)
                     /translation=""
     gene            5170..5172
                     /gene="rpoD"
                     /gene_synonym="alt; ECK3057; JW3039"
                     /locus_tag="b3067"
                     /label=rpoD
                     /db_xref="EcoGene:EG10896"
                     /db_xref="GeneID:947567"
     CDS             5170..5172
                     /codon_start=1
                     /label=BreR-Bt
                     /translation=""
     CDS             5170..5172
                     /label=CmeR(NCTC11168)
     gene            5170..5172
                     /label=rpoD
     CDS             5170..5172
                     /label=BreR-Bt
     primer_bind     5173..5197
                     /label=oMT1188
     misc_feature    5173..5182
                     /label=Amp up this region
     misc_feature    5173..5176
                     /label=CAAT
     primer_bind     5183..5197
                     /label=oMT676
     misc_feature    5183..5188
                     /label=problematic assembly region
     terminator      5189..5249
                     /label=L3S2P21 Terminator
     primer_bind     complement(5228..5268)
                     /label=oMT1272
     misc_feature    5228..5249
                     /label=Feature 67
     primer_bind     5250..5277
                     /label=oMT1259
     primer_bind     complement(5375..5395)
                     /label=pNBU1_seq.REV
     misc_feature    complement(5424..5462)
                     /label=mmD660
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    5463..7205
                     /label=intN1 Construct PCR Product - intN1 Construct P
                     /label=intN1 Construct PCR Product - intN1 Construct PCR 
                     Product 2
                     /note="Geneious type: Inserted sequence"
     misc_feature    5463..6262
                     /label=Piece1
     misc_feature    5463..5492
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
     primer_bind     5463..5491
                     /label=mmD658
     primer_bind     5484..5504
                     /label=oMT113
     misc_feature    5493..5822
                     /label=attN1 Region
     primer_bind     5493..5512
                     /label=intN1 Construct PCR Product (fwd) - pNBU2-PcfxA
                     /label=intN1 Construct PCR Product (fwd) - 
                     pNBU2-PcfxA-NanoLuc PCR Product
     misc_feature    5543..5558
                     /label=DR1
     misc_feature    5576..5590
                     /label=IR1
     misc_feature    5600..5614
                     /label=IR2
     misc_feature    5620..5633
                     /label=CC Region
     misc_feature    5632
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     misc_feature    5632
                     /label=G-->C
     misc_feature    5633
                     /label='-1'
     misc_feature    5634
                     /label='+1'
     misc_feature    5651..5666
                     /label=DR2
     primer_bind     complement(5689..5711)
                     /label=oMT114
     misc_feature    5775..5791
                     /label=DR3
     gene            5838..6262
                     /gene="intN1"
                     /label=intN1
                     /label=intN1 gene
     CDS             5838..6262
                     /codon_start=1
                     /transl_table=11
                     /gene="intN1"
                     /product="IntN1"
                     /label=intN1
                     /label=intN1 CDS
                     /note="NBU1 integrase"
                     /db_xref="GI:8308020"
                     /protein_id="AAF74437.1"
                     /translation="MKVTFIIKKAAKRYDTESMATIYVRFRNGRQLDSVAPTQLAINPN
                     LWDDKDECVKTKAVCNEEMRTHINEEIRQLKTYIEKVYQQEKEAIDKEWLKTTLDKFYH
                     PEKYFLPEEVVIKPTIGELFDEFLNKHPLSEVRKKNF"
     primer_bind     6054..6081
                     /label=oMT156
     primer_bind     complement(6054..6081)
                     /label=oMT157
     misc_feature    complement(6232..6262)
                     /label=mmD659
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    6233..7205
                     /label=Piece2
     gene            6233..7175
                     /gene="intN1"
                     /label=intN1
                     /label=intN1 gene
     CDS             6233..7175
                     /codon_start=1
                     /transl_table=11
                     /gene="intN1"
                     /product="IntN1"
                     /label=intN1
                     /label=intN1 CDS
                     /note="NBU1 integrase"
                     /db_xref="GI:8308020"
                     /protein_id="AAF74437.1"
                     /translation="MVGSTKEKFPGCQKSLTAL*TICKGYKERTKGLYP*CGFGNT*HA
                     SGYVGFLSERIPVL*TLPEHL*SHSRKEDTTAQKQKHADRLFFKNTHILPVVLR*QTHH
                     KQTFRQVSDRGVYIWYTLLYNTRRKGQDF*CRPFCHPTTGNTEGYIHISDTDRMQGERP
                     VPNDQTKCGQ*SHRIYSQENQRGESGYGTCST*RQSERNP*TLQRI*GKTVAVHIRAKV
                     Q*CHKKDIQISWS*PHRNNLRPVDAQRNQTTYL*SGKQPSGKTYVYRQYL*KSERPEPC
                     FRTVGTQGGKQSFQTIQGY*RRNEERPCKTTGL"
     misc_feature    6233..6262
                     /label=Ligation
     misc_feature    6233..6262
                     /label=Piece2
     primer_bind     6233..6252
                     /label=intN1 Construct PCR Product 2 (fwd)
     misc_feature    complement(6238..6262)
                     /label=intN1 Construct PCR Product (rev)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(7151..7177)
                     /label=intN1 Construct PCR Product 2 (rev) - pNBU2-Pcf
                     /label=intN1 Construct PCR Product 2 (rev) - 
                     pNBU2-PcfxA-NanoLuc PCR Product
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    7167
                     /label=T
                     /note="Geneious type: Editing History Replacement"
     misc_feature    complement(7169..7205)
                     /label=mmD661
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    7169
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    7176..7205
                     /label=HOMOLOGY
     misc_feature    7178..7205
                     /label=CTTGTAGTTCGTTTAATTCGCTTTTCGTTTTTACGAATTTAACGAAT
                     /label=CTTGTAGTTCGTTTAATTCGCTTTTCGTTTTTACGAATTTAACGAATTGA 
                     ATATCATTGTTTTACGATTGATTTTCATTATATATTTGCGCCACTATCCTAATCGGGG 
                     TAGTGGCTTTTCGTTTATAATACTGCCGAAACGGGAA
                     /note="Geneious type: Editing History Replacement"
ORIGIN
        1 agagtagcga tattaaaaga atccgatgag aaaagactaa tatttatcta tccattcagt
       61 ttgatttttc aggactttac atcgtcctga aagtatttgt tggtaccggt accgaggacg
      121 cgtaaacatt tacagttgca tgtggcctat tgtttttagc cgttaaatat tttataacta
      181 ttaaatagcg atacaaattg ttcgaaacta atattgttta tatcatatat tctcgcatgt
      241 tttaaagctt tattaaattg attttttgta aacagttttt cgtactcttt gttaacccat
      301 ttcattacaa aagtttcata tttttttctc tctttaaatg ccatttttgc tggctttctt
      361 tttaatacaa ttaatgtgct atccacttta ggttttggat ggaaataata cctaggaatt
      421 tttgctaata tagaaatatc tacctctgcc attaacagca atgctagtga tctgtttgta
      481 tctaataaca ttttagcaaa accatattcc actattaaat aacttattgt ggctgaactt
      541 tcaaaaacaa tttttcgaat tatatttgtg cttatgttgt aaggtatgct gccaaatatt
      601 ttatatggat tgtggctagg aaatgtaaat ttcagtatat catcatttac tatttgatag
      661 ttaggataat ttaagagctt attacgagtt acctcacata atttagaatc aatttctatc
      721 gccgttacaa aattacatct ctttaccaat ccagcagtaa aatgaccttt ccctgcacct
      781 atttcaaaga tgttatcttt ttcatctaaa cttatgcaat tcattatttt ttctatgtga
      841 tattttgaag taataaaatt ttgactatct tttatattta ctttgttcat tataacctct
      901 ccttaattta ttgcatctct tttcgaatat ttatgttttt tgagaaaaga acgtactcat
      961 ggttcatccc gatatgcgta tcggtctgta tatcagcaac tttctatgtg tttcaactac
     1021 aatagtcatc tattctcatc tttctgagtc caccccctgc aaagcccctc tttacgacat
     1081 aaaaattcgg tcggaaaagg tatgcaaaag atgtttctct ctttaagaga aactcttcgg
     1141 gatgcaaaaa tatgaaaata actccaattc accaaattat atagcgactt ttttacaaaa
     1201 tgctaaaatt tgttgatttc cgtcaagcaa ttgttgagca aaaatgtctt ttacgataaa
     1261 atgatacctc aatatcaact gtttagcaaa acgatatttc tcttaaagag agaaacacct
     1321 ttttgttcac caatccccga cttttaatcc cgcggccatg attgaaaaag gaagagtatg
     1381 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1441 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     1501 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     1561 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     1621 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     1681 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     1741 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     1801 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     1861 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     1921 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     1981 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2041 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtcacgc
     2101 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2161 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     2221 ctgattaagc attggtaact gtcagaccaa gtttactcat aacgcgtcaa ttcgaggggg
     2281 atcaattccg tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc
     2341 ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg
     2401 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct
     2461 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
     2521 ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata
     2581 atgaccccga agcagggtta tgcagcggaa aacggaattg atccggccac gatgcgtccg
     2641 gcgtagagga tctgaagatc agcagttcaa cctgttgata gtacgtacta agctctcatg
     2701 tttcacgtac taagctctca tgtttaacgt actaagctct catgtttaac gaactaaacc
     2761 ctcatggcta acgtactaag ctctcatggc taacgtacta agctctcatg tttcacgtac
     2821 taagctctca tgtttgaaca ataaaattaa tataaatcag caacttaaat agcctctaag
     2881 gttttaagtt ttataagaaa aaaaagaata tataaggctt ttaaagcttt taaggtttaa
     2941 cggttgtgga caacaagcca gggatgtaac gcactgagaa gcccttagag cctctcaaag
     3001 caattttgag tgacacagga acacttaacg gctgacatgg gaattcccct ccaccgcggt
     3061 ggattaatgc ggctgcaatt tttttgggcg gggccgccca aaaaaatcct agcaccctgc
     3121 agggtctccc gctttacaaa gaaaattcga caaactgtta tttttctatc tatttaaatg
     3181 tacacccgtt tgtttacttt actttagtta tgtacctttg tcggcaatgt acacccgaaa
     3241 gtttactttc aaagataaaa tggtttagtg ttttagagct agaaatagca agttaaaata
     3301 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttggatcccc
     3361 aattattgaa caccctaacg ggtgtttttt tttttttggt ctcccgcggc cgcgccgcgg
     3421 ccgctgctgt cataggccgg ttgtccggta gtccggatga tttaaggaag atttctcttc
     3481 ttttttcact ttctccgaac aacctccaac taaaccattt tatctttgca gtagctgtca
     3541 ccggatgtgc tttccggtct gatgagtccg tgaggacgaa acagcctcta caaataattt
     3601 tgtttaacgc attttaaaat aaaataaatt atttatttaa ttaaacgaat atggttttta
     3661 ctctggaaga ttttgttggc gattggcgtc agaccgcggg ttataatttg gatcaagtcc
     3721 tggaacaggg tggcgtaagc tctctgttcc agaacctggg tgtgagcgtg acgccgattc
     3781 agcgcatcgt tctgtccggc gagaacggtc tgaaaattga tattcatgtg atcatcccgt
     3841 acgaaggcct gagcggtgac caaatgggtc aaatcgagaa aatctttaaa gtcgtctacc
     3901 cagttgacga tcaccacttc aaggttatct tgcattacgg tacgctggtg attgatggtg
     3961 tgaccccgaa tatgattgac tatttcggcc gtccgtatga aggcattgcc gtttttgacg
     4021 gtaaaaagat caccgtcacc ggtaccctgt ggaatggcaa taagattatt gacgagcgtc
     4081 tgattaaccc ggacggcagc ctgctgttcc gcgtgaccat caacggtgtc acgggttggc
     4141 gtctgtgcga gcgcatcctg gcataatgaa ctgggatccc tcggtaccaa attttcgaaa
     4201 aaagacgctg aaaagcgtct tttttcgttt tggtccgcgg ccgccggccg ctgatctgga
     4261 agaagcaatg aaagctgctg ttaagtctcc gaatcaggta ttgttcctga caggtgtatt
     4321 cccatccggt aaacgcggat actttgcagt tgatctgact caggaataaa ttataaatta
     4381 aggtaagaag attgtaggat aagctaatga aatagaaaaa ggatgccgtc acacaacttg
     4441 tcggcattct tttttgtttt attagttgaa aatatagtga aaaagttgcc taaatatgta
     4501 tgttaacaaa ttatttgtcg taactttgca ctccaaatct gtttttaaca tatggcacta
     4561 atgcagaaaa aattgactcg ttctcagcag aaacacttgg acatcatcaa cgctgctaaa
     4621 gaagaattca tcgaattcgg tttcttggct gctaacatgg accgtatcac ttcttctgct
     4681 gaagtatcta aacgtacttt gtaccgtcac ttcgaatcta aagaagtatt gttcgaatct
     4741 gtattgacta tcatcaacga ctctgtaaac gaatctatct cttaccactt cgacccgaac
     4801 aaatctactg aagaacagtt gactgaaatc gcttacaaag aaatcgacgt attgtacaaa
     4861 acttacggta tcgctttggc tcgtactatc gtaatggaat tcttgcgtca gccggaaatg
     4921 gctaaaactt tgatccagaa catctactct atccgtgcta tcactcagtg gttccgttct
     4981 gctatcgaag ctaaacgttt gaaagacgct gacccgaaat tgatgactga cgtatacgta
     5041 tctttgttcc agggtttgtt cttctggccg caggtaatgc acttggactt ggaaccgcac
     5101 ggtgaagaat tgtctcagaa aatcgaaact ttgactacta tcttcttgca gtcttacggt
     5161 gtagctgaat aacaatgaat gggctttcct cggtaccaaa ttccagaaaa gaggcctccc
     5221 gaaagggggg ccttttttcg ttttggtccc taattgccta tcttccagtg atggaacagc
     5281 atttgtgcat tggctgcaac aatcagcctt acttgtgcct gttctatttc cgaaccgacc
     5341 gcttgtatga atccatcaaa attcgttttc tctatgttgg attccttgtt gctcatattg
     5401 tgatgataat ttctacaaat atagtcattg gtaactatct atgaaactgt ttgatacttt
     5461 tactcatcgg attcttttaa tatcgctact ctctacgttc aaccaaaaga aataatgact
     5521 tactgctata ttttttgcac gtgtggggaa aatgtgggga aaattcaagc aaaagaaaaa
     5581 gctaagtatt gaactatcaa atacttagct ttctttcttg tacccagacc ccgcatttga
     5641 aataattaaa gtggggaaaa tgtgggtaaa aagaaaaatg cggaaaaacg ccacaattac
     5701 actgtatttc aatatgttat aatcctatta aattttaatc caagtttaat cgaattgcaa
     5761 aatatttagc agatgtgggg aaaatgctgg ggaaaatatt tatatttgca gcagagtaaa
     5821 attcaacctg aaagtctatg aaagtaacct ttatcattaa aaaagcagcc aaacgatatg
     5881 atacagaatc catggctaca atctatgtcc gttttagaaa cggaaggcag ttagactccg
     5941 ttgctcctac tcagttagcc atcaatccca atctatggga tgataaagac gaatgtgtaa
     6001 aaacgaaagc tgtctgcaat gaagaaatgc gtacccatat aaatgaagag atacgccagt
     6061 tgaaaaccta tatcgagaag gtatatcaac aagaaaagga agcaatagac aaagaatggc
     6121 taaaaacaac acttgataaa ttttaccatc ctgaaaaata ttttttgccg gaggaagtgg
     6181 ttatcaagcc taccattgga gaactattcg atgaatttct aaacaagcac cctttgtcgg
     6241 aagtacgaaa gaaaaatttc cgggttgtca aaagagcctt actgcgttat gaactatatg
     6301 taagggctac aaagagagga caaaagggct ttatccttga tgtggatttg gtaacacctg
     6361 acacgcttcg ggatatgtgg gatttctttc agaacgaata ccagtattat gaactttacc
     6421 cgagcattta tgaagccatt cccgaaaaga ggacaccaca gcccagaagc aaaaacacgc
     6481 tgatagactg tttttcaaga atacgcacat tcttcctgtg gtgcttcgat aacaaacgca
     6541 ccacaaacag acctttcgac aagtttccga tagaggagtg tacatatggt acaccttatt
     6601 atataacact cgaagaaagg gacaggattt ttaatgcaga cctttctgcc accccacaac
     6661 tggcaataca gagggatata ttcatatttc agacactgat aggatgcagg gtgagcgacc
     6721 tgtaccgaat gaccaaacta aatgtggtca atgaagccat agaatatatt cccaagaaaa
     6781 ccaaagaggg gaatccggtt acggtacgtg ttccacttaa cgacaaagcg aaagaaatcc
     6841 ttgaacgcta caaagaatat gagggaaaac tgttgccgtt catatccgag caaaagtaca
     6901 atgatgccat aaaaaagata ttcaaattag ctggagttga ccgcatcgta acaatcttag
     6961 acccgttgac gcacaacgaa atcaaacgac ctatttatga agtggcaagc agccatctgg
     7021 caagacgtac gtttatcggc aatatctata aaaaagtgaa agacccgaac cttgtttccg
     7081 cactgtcggg acacaaggag ggaagcaaag ctttcagacg atacagggat attgacgaag
     7141 aaatgaagaa agaccttgta aaactactgg actgataaaa gtatcaaaca gtttcataga
     7201 tagtt
//
