LOCUS       pGWKS5                  8393 bp    DNA     circular SYN 11-Oct-2017
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     misc_feature    complement(1..750)
                     /note="Safe Haven (CNAG_00778 side)"
     CDS             complement(751..770)
                     /note="spacer"
     terminator      complement(751..1018)
                     /note="TRP1 terminator"
     CDS             751..2433
                     /note="NAT cassette"
     CDS             complement(1019..1600)
     promoter        complement(1601..2433)
                     /note="ACT1 promoter"
     gene            complement(join(2570..>2783,<5373..5458))
                     /note="lacZ [Split]"
     misc_feature    join(2763..>2783,<5373..5402)
                     /note="MCS [Split]"
     regulatory      2784..4005
                     /note="TEF1promoter"
     CDS             4006..4722
                     /note="mCitrine codon optimised"
     regulatory      4723..5366
                     /note="ADE6 terminator"
     misc_feature    <5367..>5372
     promoter        5459..5580
                     /note="lac promoter"
     rep_origin      5800..6467
                     /note="pUC origin"
     gene            6618..7475
                     /note="bla"
     misc_feature    complement(7616..8365)
                     /note="Safe Haven (CNAG_00777 side)"
     misc_feature    complement(8366..8393)
                     /note="Linker sequence"
BASE COUNT      2016 A      2208 C      2055 G      2114 T         0 OTHER
ORIGIN      ?
        1 ACCAAACCAG TCTGTGCCAA GTGGATTGCT TATGCATCGA GTGGCGAGCC CACTGACCTC
       61 TGGGCCCCTT TTTTGAACTA TAAAAAGCGG CCAAGTCTGA TTTCGGTCTC ACAATCATCA
      121 ACATCGACTT CTCTTTTCGC CTCGATCAAC CTAAACAACG GTCACTCAGC CATGAACTGG
      181 ACAATGGAAT ATTTCGCCTT GCCTTGGATC TTTGGCCTCT AGACTCAATC AACGTCAGGA
      241 TAAAGGTGAG CATAGGTTGC TCACTCACTG TAGCCTTTTT TTCCCTTCTT CTTGGTCCAG
      301 ATGGTTCCCA AGGCTCTATA AAGTGTATCT GTCAGTTCCA CTTCCTTACT CCGAAGCGAT
      361 TCAGAGTTGT AAAGTGCGTC TGACCCCCTG ATTATATCTC AAAGTCCCTT CACAAATTCA
      421 GACATGATGC GATAAAGTGC CGGATTGTCA TTCGCGTCTT GTGACTGTAT AGAAAGTGCC
      481 GGCATCCTCT TTTCTTTTCC TCTTTGGCCG AGCGACTCAG AGTTGTGAAG CCTGCTGGTA
      541 TCTGTCTGTT CCCTTGCCCT GATAAAGAAT ATCGAAGAGC ATTTCTTTGA TTCACAATAC
      601 GTTGACGAAT GGTGTGATGG ATCCTAAGCA GCGCCAGCCC TTTGAACTTG TGTCGAAAAA
      661 AAAAAAAAAA CAGCATATGA TACGTAAAGG GAAGAAAAGG ATGGAGCTGA CTGTTTTGAC
      721 AGGCATAATT TTGAGCGTGC TTCATTGGCC GGTTTATCTG TATTAACACG GAAGAGATGT
      781 AGAAACTAGC TTCCTGGTTT CAGAGACAAG GAGCCATGAA GATCCTGAGG ATTCAACGGC
      841 GTTGCTGAGG GTGGGAAGGA TGGTATTGTT GTCTGTAGAT AGATTAATGG GCATATAATG
      901 TCAAAAAAGA TGATTGACAC GATGTCTGTC TCGAATTCGC AGTGTGCTAA AATTGTATCA
      961 CTTCTCTAAT ATGTATATAT ACACCCTCTA AGGAAAATTA ACCCCTTACC GCCTTCACGA
     1021 ATTCTTAGGG GCAGGGCATG CTCATGTAGA GCGCCTGCTC GCCGTCCGAG GCGGTGCCGT
     1081 CGTACAGGGC GGTGTCCAGG CCGCAGAGGG TGAACCCCAT CCGCCGGTAC GCGTGGATAG
     1141 CCGGTGCGTT AACGTTGGTG ACCTCCAGCC AGAGGTGCCC GGCGCCCCGC TCGCGGGCGA
     1201 ACTCCGTCGC GAGCCCCATC AACGCGCGCC CGACCCCGTG CCCCCGGTGC TCCGGGGCGA
     1261 CCTCGATGTC CTCGACGGTC AGCCGGCGGT TCCAGCCGGA GTACGAGACG ACCACGAAGC
     1321 CCGCCAGGTC GCCGTCGTCC CCGTACGCGA CGAACGTCCG GGAGTCCGGG TCGCCGTCCT
     1381 CCCCGTCGTC CGATTCGTCG TCCGATTCGT CGTCGGGGAA CACCTTGGTC AGGGGCGGGT
     1441 CCACCGGCAC CTCCCGCAGG GTGAAGCCGT CCCCGGTGGC GGTGACGCGG AAGACGGTGT
     1501 CGGTGGTGAA GGACCCATCC AGTGCCTCGA TGGCCTCGGC GTCCCCCGGG ACACTGGTGC
     1561 GGTACCGGTA AGCCGTGTCG TCAAGAGTGG CGGCCGCCAT AGACATGTTG GGCGAGTTTA
     1621 CTAATGGAAA AAGACAATTA GCAAAGAGGC TGCATATTAG GAGACGCGGT GAAGATTGGG
     1681 GAGGACAATA GGGTCCGTGC ACCAAAAAGT CCTGACGGCA GCTCTGTTCA ACGCACATCC
     1741 ATCTCTTCCA GCTATGCGTG TGGCACCAGC ACGGATGGAT TGTTGCACCA AAAGGACAAG
     1801 GCGAGATGGA TGCAGGTCGA GGCGATGCGG TGCTATCGGC CCTCGTTGTC GAACGCCACG
     1861 TCGTCCTCTC GGCGACCGTC AATCCTGGAA GGAAATGACC AAGCGTGCAC CCAGCATCCA
     1921 GCATCCAACA TCCAGCACCC AGCACCTAGC ACCCATGATC CAATGGCCGG CATGCAGATC
     1981 AGCGTGGGGA GAGCACCATC TAGCAGCCCC TGGCAGGCCC AGCATTGTGC TCGGATGTGC
     2041 GATAGGCGGT GGGTGCGATA AGGTGGATTA GTGGAGGATG GAGGACTGAG TGGCGGGCAA
     2101 GGGAAGGGGG CGGGAAGATG ACTCACCTTG GGCAGTGGGT GGGGGATGCG GTATAGGGGA
     2161 TGTATGTGGA TGCTGGCGGA GGATAGAAGC TGTAAGCTGA GCTGGTGCCA ACGGTGACGC
     2221 TGTGAGAGTG GTCTCGGCGA CCCTGACGGC GACTCGGCGT TACCCCGTGT GGCACACCGG
     2281 ACGCGCGTCG AGTGACGTTG CTGCTATTGT TGTTACCATC ATCCTCTCCT CCGACAACCA
     2341 TACACTCAGT GCACACCATG CCACTCGAAT CCTGCATGCT TATGTGAGTC CTCCCCTTCC
     2401 CGTGCGCCGC TCTCCAGCTC ACATCCTCGC AGCCATGGAC TCAACCCTAT CTCGGTCTAT
     2461 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT
     2521 TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGC TTACAATTTC CATTCGCCAT
     2581 TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC
     2641 TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT
     2701 CACGACGTTG TAAAACGACG GCCAGTGAGC GCGCGTAATA CGACTCACTA TAGGGCGAAT
     2761 TGGGTACCGG GCCCCCCCTC GAGCATTCTA TGCTATACGG TACAAGATTG TGGCTACTAT
     2821 GCAAGCTCCA TAGCGAATGA TCTTACACTG ACCTTGTCGA CCGTTCTGAA TGATTAACAA
     2881 TGGTCGCAAG TGTGGTTGAC CCACCTGCGG GCGCTTCTTA ACTATCTATC ATCGGTATGT
     2941 CAAGTATCAC TCCCTTTAAA CAAAAGAATA AGAGCATAAT ACAGCAGTCT CATCATGGCA
     3001 ACCCTTCGCG CAATGTACAA AAAGCAGCCA GAAGCCCCCA CGCACAAGCA ATAATGTCGT
     3061 GTTCTACCCT GCCATGAACA AGCGTCATGG CCATTAGCGA ACGCCCCAAT ATTCAGCCCC
     3121 ATTCGCCAAG CAAGTTGCCT CGCCCCCTTC CCAGAAGATC ATGGGCATCT CGTCTTCTTC
     3181 TAATTTCGCA TTTTGTGAAG CTCCATTCAT GCACGTCGGC TTCTGGACGA CTCTTATCAT
     3241 ATGCATTGGC CTGCTCGGTG TGAGGATGAG CCACTCCCAC ATGTTTAGAC GGCAGGGGCC
     3301 TCTTGCCACC CGTTGAATTT TTGGTGCGAA GACGTGCCTG GGAACGACCT CCACTTTAAA
     3361 TTTCTCCGTT GTTGCGGTCG CTCCATCCTC CTCACATTTC TTGCCTCCTA AGTCCCATGT
     3421 GCCACACTCG GTGAAAAACG GAGTTTGATA CGGATGAAAT TTCCAGTGAG CTTTTCACAT
     3481 TCTGGAGGTT TCGAAAAAAA TTGTGGGATT TTCTGAATTA ATCCGAGAAT CCGCCCTGCC
     3541 CATCAACCGG AAAGAATTAA ATCCCTCGCA CATTCCTTTC TCCCACCACC TTCTTTATCT
     3601 CTACATTCTT TAACCAAAAC ACCTAGGTGA GTACCCAAAG TCTTTTGACA CCTTTGAAAA
     3661 TCCGCGCCAG TCGTAGGGCA CGTAAAACAA TTCTCGCTGG TGGCTTCTGC AGGTGAAGTC
     3721 GGGGCAGAGT GGAGCTGAGC ATGGTCGACC ACCCACATCA TTCTGTCTTG TTTTTGGCGA
     3781 TGTCTTCCAT GCTTTTGCCC TCATCTGTTG CGTCGACTGC AGAGTACTGT TTTGCCACTA
     3841 CTGAGCTCAA CATGGGCTTG TGTGTGGTGA AGAAAAAAAT TTGGTGGCTC TTGAAAATTT
     3901 TCAACCCATC GGATACATGC CCTTCCAACT CACAACGCCC TAACACTTTA TTGTCCTATC
     3961 ATTGCCTCTT CGCACATGCT AACGATCTCC ACAGAAAACT TCAAAATGGT CTCCAAGGGC
     4021 GAGGAGCTCT TCACCGGCGT CGTCCCCATC CTCGTCGAGC TCGACGGCGA CGTTAACGGC
     4081 CACAAGTTCT CCGTCTCCGG CGAGGGCGAG GGCGACGCCA CCTACGGCAA GCTCACCCTC
     4141 AAGTTCATCT GCACCACCGG CAAGCTCCCC GTCCCCTGGC CCACCCTCGT CACCACCTTC
     4201 GGCTACGGCC TCATGTGCTT CGCCCGTTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
     4261 AAGTCCGCCA TGCCCGAGGG CTACGTCCAG GAGCGTACCA TCTTCTTCAA GGACGACGGC
     4321 AACTACAAGA CCCGTGCCGA GGTCAAGTTC GAGGGCGACA CCCTCGTCAA CCGTATCGAG
     4381 CTCAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTCG GCCACAAGCT CGAGTACAAC
     4441 TACAACTCCC ACAACGTCTA CATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTCAAC
     4501 TTCAAGATCC GTCACAACAT CGAGGACGGC TCCGTCCAGC TCGCCGACCA CTACCAGCAG
     4561 AACACCCCCA TCGGCGACGG CCCCGTCCTC CTCCCCGACA ACCACTACCT CTCCTACCAG
     4621 TCCGCCCTCT CCAAGGACCC CAACGAGAAG CGTGACCACA TGGTCCTCCT CGAGTTCGTC
     4681 ACCGCCGCCG GCATCACTCT CGGCATGGAC GAGCTTTACA AGTAGAAGGT TGAGCGCGTA
     4741 ATAGTGGGTT GGTCTTGTCT CTATCCATTC ATCTGTGAGA CATACTGATT TCAACTTTTT
     4801 CATCAGATAA TAAGGATGGT GTGAGATAAG CAAGGCTGGG TTATGAGGTT TGGGACTAGA
     4861 TGGTGTTTCG CGTCTAAAGG TGAGTCTTCG TTGGTTCAAT CACATTGATC TGATCGCTGA
     4921 TCAATTGTCA GCCTCTGAAT GTGCTCTTGA AGAAAATGGT TGTCTGTAAG AATCCAGAAC
     4981 CAAAAACGGT CATTTCAAGT ATGTATCCTC CGTGAAGTTC CTTTAGCTCT TGCTAATATT
     5041 GGACTTTAGC GTGCTTTAGC CAAAGACAGG GTCGCGAGGT TAAGAGGATG GGCCAGGAAA
     5101 AATCTAAGTT AGATTTGTCA CACAAAGAAA AAGATTGTCT CAGTTTTGGT TTTGTTTGGT
     5161 TTGATTTATG CATAGGAAAT ATTTATGATC GAAATCACTC ATTTCCTGCT TCGTAAATAT
     5221 CATCTACTAT ATGCTTCGTA AATGTCCATC TCCTACATAT TGTGGATATG ATAAATGCAA
     5281 TCCTCTCGTC TACACTCTCG GCTTATGCCT TTGTCTTCTT TGCGCTCCTT TTAGCCTTCT
     5341 TTTTATTCCT AGTCTTCAAG CCCATCACTA GTTCTAGAGC GGCCGCCACC GCGGTGGAGC
     5401 TCCAGCTTTT GTTCCCTTTA GTGAGGGTTA ATTGCGCGCT TGGCGTAATC ATGGTCATAG
     5461 CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC
     5521 ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC
     5581 TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA
     5641 CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG
     5701 CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG
     5761 TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG
     5821 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC
     5881 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA
     5941 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT
     6001 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC
     6061 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC
     6121 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA
     6181 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT
     6241 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA
     6301 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT
     6361 TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT
     6421 ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT
     6481 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC
     6541 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA
     6601 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA
     6661 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC
     6721 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT
     6781 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA
     6841 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT
     6901 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT
     6961 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG
     7021 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC
     7081 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC
     7141 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG
     7201 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA
     7261 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA
     7321 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT
     7381 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG
     7441 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA
     7501 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT
     7561 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTCATCA GATCTGCCCA
     7621 TAGACTTCAA ATCATGGAAT TGGACGTTTA TCTTCTTGCT TTGGATATCC ACCCTATCGT
     7681 ACAATCTTGG CACTTTGGAT CATACGAGGC ACTTTGCGGA CTAGGGAATG GTTATCATCA
     7741 ATTTCTTGGA CCTCTCCATG ACACCTGGCA TCGTCGAGCT ACCACAGCCC CCTGAATTCT
     7801 TAAAGGCGAA CCCCCCTTCC TAGGAACCTT GAGCGCTGGA CTGGATGGCA CTCTGAGACC
     7861 GCCTATGCTT AAGGACGCGA GCTTACCCTT CCATTGATGG AAGTCGATCA TTTATGATAA
     7921 TAGAACACGG CGGAACTTGA CTTTACAGGC CAAGGGAAGC AACAGGAGAA GAAGCTAAGG
     7981 TTATAACAGG CTCAGCATAT TTGTATATAC GGTGCCATTC AACGCTGAAC ACTTTGTACT
     8041 CACGGTCTGC CCGTTGAATA ACACCATCTG CTGATGATCA TCCCAATTTG TGAGTCTTAT
     8101 TTGCCATTGA TCCTGGTGTA TAACCGATAC TGAAGCCCTT TAGGTCTGAT CAGTTATAGA
     8161 CGTGGTATAT TCATTCTTCC TAGATTAGCT ATGCCGAAAG AGATGGATAC CTTGATGTGA
     8221 CTGGAATGCC ATGAAGCAGG AGATTGTAGC AGCTCAAATT GGAGAAGGAT GTTGCAACCG
     8281 TCGGCCGTGA AGGTCTTGTC ATATCTTTGT CTGCCTGGGA GAATATGTAC AAGTGCCCCG
     8341 ATGTTACTGA TGGAAAACAT CGTGTGGCGC GCCACGATAC TTGTGTTAAT TAA
//
