LOCUS       pGWKS8                  8384 bp    DNA     circular SYN 11-Oct-2017
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     misc_feature    complement(1..750)
                     /note="Safe Haven (CNAG_00778 side)"
     regulatory      complement(751..770)
                     /note="spacer"
     terminator      complement(751..1018)
                     /note="TRP1 terminator"
     CDS             751..2433
                     /note="NAT cassette"
     CDS             complement(1019..1600)
     promoter        complement(1601..2433)
                     /note="ACT1 promoter"
     gene            complement(join(2570..>2783,<5364..5449))
                     /note="lacZ [Split]"
     misc_feature    join(2763..>2783,<5364..5393)
                     /note="MCS [Split]"
     regulatory      2784..4005
                     /note="TEF1 promoter"
     CDS             4006..>4713
                     /note="mTFP1"
     regulatory      4714..5357
                     /note="ADE6 terminator"
     promoter        5450..5571
                     /note="lac promoter"
     rep_origin      5791..6458
                     /note="pUC origin"
     gene            6609..7466
                     /note="bla"
     misc_feature    complement(7607..8356)
                     /note="Safe Haven (CNAG_00777 side)"
     misc_feature    complement(8357..8384)
                     /note="Linker sequence"
BASE COUNT      2028 A      2181 C      2067 G      2108 T         0 OTHER
ORIGIN      ?
        1 ACCAAACCAG TCTGTGCCAA GTGGATTGCT TATGCATCGA GTGGCGAGCC CACTGACCTC
       61 TGGGCCCCTT TTTTGAACTA TAAAAAGCGG CCAAGTCTGA TTTCGGTCTC ACAATCATCA
      121 ACATCGACTT CTCTTTTCGC CTCGATCAAC CTAAACAACG GTCACTCAGC CATGAACTGG
      181 ACAATGGAAT ATTTCGCCTT GCCTTGGATC TTTGGCCTCT AGACTCAATC AACGTCAGGA
      241 TAAAGGTGAG CATAGGTTGC TCACTCACTG TAGCCTTTTT TTCCCTTCTT CTTGGTCCAG
      301 ATGGTTCCCA AGGCTCTATA AAGTGTATCT GTCAGTTCCA CTTCCTTACT CCGAAGCGAT
      361 TCAGAGTTGT AAAGTGCGTC TGACCCCCTG ATTATATCTC AAAGTCCCTT CACAAATTCA
      421 GACATGATGC GATAAAGTGC CGGATTGTCA TTCGCGTCTT GTGACTGTAT AGAAAGTGCC
      481 GGCATCCTCT TTTCTTTTCC TCTTTGGCCG AGCGACTCAG AGTTGTGAAG CCTGCTGGTA
      541 TCTGTCTGTT CCCTTGCCCT GATAAAGAAT ATCGAAGAGC ATTTCTTTGA TTCACAATAC
      601 GTTGACGAAT GGTGTGATGG ATCCTAAGCA GCGCCAGCCC TTTGAACTTG TGTCGAAAAA
      661 AAAAAAAAAA CAGCATATGA TACGTAAAGG GAAGAAAAGG ATGGAGCTGA CTGTTTTGAC
      721 AGGCATAATT TTGAGCGTGC TTCATTGGCC GGTTTATCTG TATTAACACG GAAGAGATGT
      781 AGAAACTAGC TTCCTGGTTT CAGAGACAAG GAGCCATGAA GATCCTGAGG ATTCAACGGC
      841 GTTGCTGAGG GTGGGAAGGA TGGTATTGTT GTCTGTAGAT AGATTAATGG GCATATAATG
      901 TCAAAAAAGA TGATTGACAC GATGTCTGTC TCGAATTCGC AGTGTGCTAA AATTGTATCA
      961 CTTCTCTAAT ATGTATATAT ACACCCTCTA AGGAAAATTA ACCCCTTACC GCCTTCACGA
     1021 ATTCTTAGGG GCAGGGCATG CTCATGTAGA GCGCCTGCTC GCCGTCCGAG GCGGTGCCGT
     1081 CGTACAGGGC GGTGTCCAGG CCGCAGAGGG TGAACCCCAT CCGCCGGTAC GCGTGGATAG
     1141 CCGGTGCGTT AACGTTGGTG ACCTCCAGCC AGAGGTGCCC GGCGCCCCGC TCGCGGGCGA
     1201 ACTCCGTCGC GAGCCCCATC AACGCGCGCC CGACCCCGTG CCCCCGGTGC TCCGGGGCGA
     1261 CCTCGATGTC CTCGACGGTC AGCCGGCGGT TCCAGCCGGA GTACGAGACG ACCACGAAGC
     1321 CCGCCAGGTC GCCGTCGTCC CCGTACGCGA CGAACGTCCG GGAGTCCGGG TCGCCGTCCT
     1381 CCCCGTCGTC CGATTCGTCG TCCGATTCGT CGTCGGGGAA CACCTTGGTC AGGGGCGGGT
     1441 CCACCGGCAC CTCCCGCAGG GTGAAGCCGT CCCCGGTGGC GGTGACGCGG AAGACGGTGT
     1501 CGGTGGTGAA GGACCCATCC AGTGCCTCGA TGGCCTCGGC GTCCCCCGGG ACACTGGTGC
     1561 GGTACCGGTA AGCCGTGTCG TCAAGAGTGG CGGCCGCCAT AGACATGTTG GGCGAGTTTA
     1621 CTAATGGAAA AAGACAATTA GCAAAGAGGC TGCATATTAG GAGACGCGGT GAAGATTGGG
     1681 GAGGACAATA GGGTCCGTGC ACCAAAAAGT CCTGACGGCA GCTCTGTTCA ACGCACATCC
     1741 ATCTCTTCCA GCTATGCGTG TGGCACCAGC ACGGATGGAT TGTTGCACCA AAAGGACAAG
     1801 GCGAGATGGA TGCAGGTCGA GGCGATGCGG TGCTATCGGC CCTCGTTGTC GAACGCCACG
     1861 TCGTCCTCTC GGCGACCGTC AATCCTGGAA GGAAATGACC AAGCGTGCAC CCAGCATCCA
     1921 GCATCCAACA TCCAGCACCC AGCACCTAGC ACCCATGATC CAATGGCCGG CATGCAGATC
     1981 AGCGTGGGGA GAGCACCATC TAGCAGCCCC TGGCAGGCCC AGCATTGTGC TCGGATGTGC
     2041 GATAGGCGGT GGGTGCGATA AGGTGGATTA GTGGAGGATG GAGGACTGAG TGGCGGGCAA
     2101 GGGAAGGGGG CGGGAAGATG ACTCACCTTG GGCAGTGGGT GGGGGATGCG GTATAGGGGA
     2161 TGTATGTGGA TGCTGGCGGA GGATAGAAGC TGTAAGCTGA GCTGGTGCCA ACGGTGACGC
     2221 TGTGAGAGTG GTCTCGGCGA CCCTGACGGC GACTCGGCGT TACCCCGTGT GGCACACCGG
     2281 ACGCGCGTCG AGTGACGTTG CTGCTATTGT TGTTACCATC ATCCTCTCCT CCGACAACCA
     2341 TACACTCAGT GCACACCATG CCACTCGAAT CCTGCATGCT TATGTGAGTC CTCCCCTTCC
     2401 CGTGCGCCGC TCTCCAGCTC ACATCCTCGC AGCCATGGAC TCAACCCTAT CTCGGTCTAT
     2461 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT
     2521 TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGC TTACAATTTC CATTCGCCAT
     2581 TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC
     2641 TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT
     2701 CACGACGTTG TAAAACGACG GCCAGTGAGC GCGCGTAATA CGACTCACTA TAGGGCGAAT
     2761 TGGGTACCGG GCCCCCCCTC GAGCATTCTA TGCTATACGG TACAAGATTG TGGCTACTAT
     2821 GCAAGCTCCA TAGCGAATGA TCTTACACTG ACCTTGTCGA CCGTTCTGAA TGATTAACAA
     2881 TGGTCGCAAG TGTGGTTGAC CCACCTGCGG GCGCTTCTTA ACTATCTATC ATCGGTATGT
     2941 CAAGTATCAC TCCCTTTAAA CAAAAGAATA AGAGCATAAT ACAGCAGTCT CATCATGGCA
     3001 ACCCTTCGCG CAATGTACAA AAAGCAGCCA GAAGCCCCCA CGCACAAGCA ATAATGTCGT
     3061 GTTCTACCCT GCCATGAACA AGCGTCATGG CCATTAGCGA ACGCCCCAAT ATTCAGCCCC
     3121 ATTCGCCAAG CAAGTTGCCT CGCCCCCTTC CCAGAAGATC ATGGGCATCT CGTCTTCTTC
     3181 TAATTTCGCA TTTTGTGAAG CTCCATTCAT GCACGTCGGC TTCTGGACGA CTCTTATCAT
     3241 ATGCATTGGC CTGCTCGGTG TGAGGATGAG CCACTCCCAC ATGTTTAGAC GGCAGGGGCC
     3301 TCTTGCCACC CGTTGAATTT TTGGTGCGAA GACGTGCCTG GGAACGACCT CCACTTTAAA
     3361 TTTCTCCGTT GTTGCGGTCG CTCCATCCTC CTCACATTTC TTGCCTCCTA AGTCCCATGT
     3421 GCCACACTCG GTGAAAAACG GAGTTTGATA CGGATGAAAT TTCCAGTGAG CTTTTCACAT
     3481 TCTGGAGGTT TCGAAAAAAA TTGTGGGATT TTCTGAATTA ATCCGAGAAT CCGCCCTGCC
     3541 CATCAACCGG AAAGAATTAA ATCCCTCGCA CATTCCTTTC TCCCACCACC TTCTTTATCT
     3601 CTACATTCTT TAACCAAAAC ACCTAGGTGA GTACCCAAAG TCTTTTGACA CCTTTGAAAA
     3661 TCCGCGCCAG TCGTAGGGCA CGTAAAACAA TTCTCGCTGG TGGCTTCTGC AGGTGAAGTC
     3721 GGGGCAGAGT GGAGCTGAGC ATGGTCGACC ACCCACATCA TTCTGTCTTG TTTTTGGCGA
     3781 TGTCTTCCAT GCTTTTGCCC TCATCTGTTG CGTCGACTGC AGAGTACTGT TTTGCCACTA
     3841 CTGAGCTCAA CATGGGCTTG TGTGTGGTGA AGAAAAAAAT TTGGTGGCTC TTGAAAATTT
     3901 TCAACCCATC GGATACATGC CCTTCCAACT CACAACGCCC TAACACTTTA TTGTCCTATC
     3961 ATTGCCTCTT CGCACATGCT AACGATCTCC ACAGAAAACT TCAAAATGGT CTCCAAGGGC
     4021 GAGGAGACCA CTATGGGCGT TATCAAGCCC GACATGAAGA TCAAGCTCAA GATGGAGGGC
     4081 AACGTCAACG GCCACGCCTT CGTCATCGAG GGCGAGGGCG AGGGCAAGCC CTACGACGGC
     4141 ACCAACACCA TCAACCTCGA GGTCAAGGAG GGTGCCCCCC TCCCCTTCTC CTACGACATT
     4201 CTCACCACCG CCTTCGCCTA CGGCAACAGG GCCTTCACCA AGTACCCCGA CGACATCCCC
     4261 AACTACTTCA AGCAGTCCTT CCCCGAGGGC TACTCTTGGG AGCGTACCAT GACCTTCGAG
     4321 GACAAGGGCA TCGTCAAGGT CAAGTCCGAC ATCTCCATGG AGGAGGACTC CTTCATCTAC
     4381 GAGATCCACC TCAAGGGCGA GAACTTCCCC CCCAACGGCC CCGTCATGCA GAAGAAGACC
     4441 ACCGGCTGGG ACGCCTCCAC CGAGAGGATG TACGTCCGTG ACGGCGTCCT CAAGGGCGAC
     4501 GTCAAGCACA AGCTCCTCCT CGAGGGCGGC GGCCACCACC GTGTTGACTT CAAGACCATC
     4561 TACAGGGCCA AGAAGGCCGT CAAGCTCCCC GACTACCACT TCGTCGACCA CCGTATCGAG
     4621 ATCCTCAACC ACGACAAGGA CTACAACAAG GTCACCGTTT ACGAGTCCGC CGTCGCCCGT
     4681 AACTCCACCG ACGGCATGGA CGAGCTCTAC AAGTAGAAGG TTGAGCGCGT AATAGTGGGT
     4741 TGGTCTTGTC TCTATCCATT CATCTGTGAG ACATACTGAT TTCAACTTTT TCATCAGATA
     4801 ATAAGGATGG TGTGAGATAA GCAAGGCTGG GTTATGAGGT TTGGGACTAG ATGGTGTTTC
     4861 GCGTCTAAAG GTGAGTCTTC GTTGGTTCAA TCACATTGAT CTGATCGCTG ATCAATTGTC
     4921 AGCCTCTGAA TGTGCTCTTG AAGAAAATGG TTGTCTGTAA GAATCCAGAA CCAAAAACGG
     4981 TCATTTCAAG TATGTATCCT CCGTGAAGTT CCTTTAGCTC TTGCTAATAT TGGACTTTAG
     5041 CGTGCTTTAG CCAAAGACAG GGTCGCGAGG TTAAGAGGAT GGGCCAGGAA AAATCTAAGT
     5101 TAGATTTGTC ACACAAAGAA AAAGATTGTC TCAGTTTTGG TTTTGTTTGG TTTGATTTAT
     5161 GCATAGGAAA TATTTATGAT CGAAATCACT CATTTCCTGC TTCGTAAATA TCATCTACTA
     5221 TATGCTTCGT AAATGTCCAT CTCCTACATA TTGTGGATAT GATAAATGCA ATCCTCTCGT
     5281 CTACACTCTC GGCTTATGCC TTTGTCTTCT TTGCGCTCCT TTTAGCCTTC TTTTTATTCC
     5341 TAGTCTTCAA GCCCATCACT AGTTCTAGAG CGGCCGCCAC CGCGGTGGAG CTCCAGCTTT
     5401 TGTTCCCTTT AGTGAGGGTT AATTGCGCGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT
     5461 GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT
     5521 AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC
     5581 GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG
     5641 AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG
     5701 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA
     5761 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC
     5821 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC
     5881 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG
     5941 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC
     6001 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT
     6061 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG
     6121 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC
     6181 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT
     6241 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT
     6301 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC
     6361 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA
     6421 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC
     6481 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC
     6541 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT
     6601 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA
     6661 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT
     6721 GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA
     6781 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC
     6841 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG
     6901 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT
     6961 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA
     7021 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA
     7081 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC
     7141 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG
     7201 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA
     7261 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG
     7321 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC
     7381 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG
     7441 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT
     7501 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA
     7561 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTCATC AGATCTGCCC ATAGACTTCA
     7621 AATCATGGAA TTGGACGTTT ATCTTCTTGC TTTGGATATC CACCCTATCG TACAATCTTG
     7681 GCACTTTGGA TCATACGAGG CACTTTGCGG ACTAGGGAAT GGTTATCATC AATTTCTTGG
     7741 ACCTCTCCAT GACACCTGGC ATCGTCGAGC TACCACAGCC CCCTGAATTC TTAAAGGCGA
     7801 ACCCCCCTTC CTAGGAACCT TGAGCGCTGG ACTGGATGGC ACTCTGAGAC CGCCTATGCT
     7861 TAAGGACGCG AGCTTACCCT TCCATTGATG GAAGTCGATC ATTTATGATA ATAGAACACG
     7921 GCGGAACTTG ACTTTACAGG CCAAGGGAAG CAACAGGAGA AGAAGCTAAG GTTATAACAG
     7981 GCTCAGCATA TTTGTATATA CGGTGCCATT CAACGCTGAA CACTTTGTAC TCACGGTCTG
     8041 CCCGTTGAAT AACACCATCT GCTGATGATC ATCCCAATTT GTGAGTCTTA TTTGCCATTG
     8101 ATCCTGGTGT ATAACCGATA CTGAAGCCCT TTAGGTCTGA TCAGTTATAG ACGTGGTATA
     8161 TTCATTCTTC CTAGATTAGC TATGCCGAAA GAGATGGATA CCTTGATGTG ACTGGAATGC
     8221 CATGAAGCAG GAGATTGTAG CAGCTCAAAT TGGAGAAGGA TGTTGCAACC GTCGGCCGTG
     8281 AAGGTCTTGT CATATCTTTG TCTGCCTGGG AGAATATGTA CAAGTGCCCC GATGTTACTG
     8341 ATGGAAAACA TCGTGTGGCG CGCCACGATA CTTGTGTTAA TTAA
//
