LOCUS pGWKS9 8393 bp DNA circular SYN 10-Aug-2018 DEFINITION - ACCESSION - KEYWORDS - SOURCE - FEATURES Location/Qualifiers misc_feature complement(1..750) /note="Safe Haven (CNAG_00778 side)" regulatory complement(751..770) /note="spacer" terminator complement(751..1018) /note="TRP1 terminator" CDS 751..2433 /note="NAT cassette" CDS complement(1019..1600) promoter complement(1601..2433) /note="ACT1 promoter" gene complement(join(2570..>2783,<5373..5458)) /note="lacZ [Split]" misc_feature join(2763..>2783,<5373..5402) /note="MCS [Split]" regulatory 2784..4005 /note="TEF1 promoter" CDS 4006..4722 /note="mTurquoise2" regulatory 4723..5366 /note="ADE6 terminator" promoter 5459..5580 /note="lac promoter" rep_origin 5800..6467 /note="pUC origin" gene 6618..7475 /note="bla" misc_feature complement(7616..8365) /note="Safe Haven (CNAG_00777 side)" misc_feature complement(8366..8393) /note="Linker sequence" BASE COUNT 2015 A 2210 C 2058 G 2110 T 0 OTHER ORIGIN ? 1 ACCAAACCAG TCTGTGCCAA GTGGATTGCT TATGCATCGA GTGGCGAGCC CACTGACCTC 61 TGGGCCCCTT TTTTGAACTA TAAAAAGCGG CCAAGTCTGA TTTCGGTCTC ACAATCATCA 121 ACATCGACTT CTCTTTTCGC CTCGATCAAC CTAAACAACG GTCACTCAGC CATGAACTGG 181 ACAATGGAAT ATTTCGCCTT GCCTTGGATC TTTGGCCTCT AGACTCAATC AACGTCAGGA 241 TAAAGGTGAG CATAGGTTGC TCACTCACTG TAGCCTTTTT TTCCCTTCTT CTTGGTCCAG 301 ATGGTTCCCA AGGCTCTATA AAGTGTATCT GTCAGTTCCA CTTCCTTACT CCGAAGCGAT 361 TCAGAGTTGT AAAGTGCGTC TGACCCCCTG ATTATATCTC AAAGTCCCTT CACAAATTCA 421 GACATGATGC GATAAAGTGC CGGATTGTCA TTCGCGTCTT GTGACTGTAT AGAAAGTGCC 481 GGCATCCTCT TTTCTTTTCC TCTTTGGCCG AGCGACTCAG AGTTGTGAAG CCTGCTGGTA 541 TCTGTCTGTT CCCTTGCCCT GATAAAGAAT ATCGAAGAGC ATTTCTTTGA TTCACAATAC 601 GTTGACGAAT GGTGTGATGG ATCCTAAGCA GCGCCAGCCC TTTGAACTTG TGTCGAAAAA 661 AAAAAAAAAA CAGCATATGA TACGTAAAGG GAAGAAAAGG ATGGAGCTGA CTGTTTTGAC 721 AGGCATAATT TTGAGCGTGC TTCATTGGCC GGTTTATCTG TATTAACACG GAAGAGATGT 781 AGAAACTAGC TTCCTGGTTT CAGAGACAAG GAGCCATGAA GATCCTGAGG ATTCAACGGC 841 GTTGCTGAGG GTGGGAAGGA TGGTATTGTT GTCTGTAGAT AGATTAATGG GCATATAATG 901 TCAAAAAAGA TGATTGACAC GATGTCTGTC TCGAATTCGC AGTGTGCTAA AATTGTATCA 961 CTTCTCTAAT ATGTATATAT ACACCCTCTA AGGAAAATTA ACCCCTTACC GCCTTCACGA 1021 ATTCTTAGGG GCAGGGCATG CTCATGTAGA GCGCCTGCTC GCCGTCCGAG GCGGTGCCGT 1081 CGTACAGGGC GGTGTCCAGG CCGCAGAGGG TGAACCCCAT CCGCCGGTAC GCGTGGATAG 1141 CCGGTGCGTT AACGTTGGTG ACCTCCAGCC AGAGGTGCCC GGCGCCCCGC TCGCGGGCGA 1201 ACTCCGTCGC GAGCCCCATC AACGCGCGCC CGACCCCGTG CCCCCGGTGC TCCGGGGCGA 1261 CCTCGATGTC CTCGACGGTC AGCCGGCGGT TCCAGCCGGA GTACGAGACG ACCACGAAGC 1321 CCGCCAGGTC GCCGTCGTCC CCGTACGCGA CGAACGTCCG GGAGTCCGGG TCGCCGTCCT 1381 CCCCGTCGTC CGATTCGTCG TCCGATTCGT CGTCGGGGAA CACCTTGGTC AGGGGCGGGT 1441 CCACCGGCAC CTCCCGCAGG GTGAAGCCGT CCCCGGTGGC GGTGACGCGG AAGACGGTGT 1501 CGGTGGTGAA GGACCCATCC AGTGCCTCGA TGGCCTCGGC GTCCCCCGGG ACACTGGTGC 1561 GGTACCGGTA AGCCGTGTCG TCAAGAGTGG CGGCCGCCAT AGACATGTTG GGCGAGTTTA 1621 CTAATGGAAA AAGACAATTA GCAAAGAGGC TGCATATTAG GAGACGCGGT GAAGATTGGG 1681 GAGGACAATA GGGTCCGTGC ACCAAAAAGT CCTGACGGCA GCTCTGTTCA ACGCACATCC 1741 ATCTCTTCCA GCTATGCGTG TGGCACCAGC ACGGATGGAT TGTTGCACCA AAAGGACAAG 1801 GCGAGATGGA TGCAGGTCGA GGCGATGCGG TGCTATCGGC CCTCGTTGTC GAACGCCACG 1861 TCGTCCTCTC GGCGACCGTC AATCCTGGAA GGAAATGACC AAGCGTGCAC CCAGCATCCA 1921 GCATCCAACA TCCAGCACCC AGCACCTAGC ACCCATGATC CAATGGCCGG CATGCAGATC 1981 AGCGTGGGGA GAGCACCATC TAGCAGCCCC TGGCAGGCCC AGCATTGTGC TCGGATGTGC 2041 GATAGGCGGT GGGTGCGATA AGGTGGATTA GTGGAGGATG GAGGACTGAG TGGCGGGCAA 2101 GGGAAGGGGG CGGGAAGATG ACTCACCTTG GGCAGTGGGT GGGGGATGCG GTATAGGGGA 2161 TGTATGTGGA TGCTGGCGGA GGATAGAAGC TGTAAGCTGA GCTGGTGCCA ACGGTGACGC 2221 TGTGAGAGTG GTCTCGGCGA CCCTGACGGC GACTCGGCGT TACCCCGTGT GGCACACCGG 2281 ACGCGCGTCG AGTGACGTTG CTGCTATTGT TGTTACCATC ATCCTCTCCT CCGACAACCA 2341 TACACTCAGT GCACACCATG CCACTCGAAT CCTGCATGCT TATGTGAGTC CTCCCCTTCC 2401 CGTGCGCCGC TCTCCAGCTC ACATCCTCGC AGCCATGGAC TCAACCCTAT CTCGGTCTAT 2461 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 2521 TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGC TTACAATTTC CATTCGCCAT 2581 TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC 2641 TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT 2701 CACGACGTTG TAAAACGACG GCCAGTGAGC GCGCGTAATA CGACTCACTA TAGGGCGAAT 2761 TGGGTACCGG GCCCCCCCTC GAGCATTCTA TGCTATACGG TACAAGATTG TGGCTACTAT 2821 GCAAGCTCCA TAGCGAATGA TCTTACACTG ACCTTGTCGA CCGTTCTGAA TGATTAACAA 2881 TGGTCGCAAG TGTGGTTGAC CCACCTGCGG GCGCTTCTTA ACTATCTATC ATCGGTATGT 2941 CAAGTATCAC TCCCTTTAAA CAAAAGAATA AGAGCATAAT ACAGCAGTCT CATCATGGCA 3001 ACCCTTCGCG CAATGTACAA AAAGCAGCCA GAAGCCCCCA CGCACAAGCA ATAATGTCGT 3061 GTTCTACCCT GCCATGAACA AGCGTCATGG CCATTAGCGA ACGCCCCAAT ATTCAGCCCC 3121 ATTCGCCAAG CAAGTTGCCT CGCCCCCTTC CCAGAAGATC ATGGGCATCT CGTCTTCTTC 3181 TAATTTCGCA TTTTGTGAAG CTCCATTCAT GCACGTCGGC TTCTGGACGA CTCTTATCAT 3241 ATGCATTGGC CTGCTCGGTG TGAGGATGAG CCACTCCCAC ATGTTTAGAC GGCAGGGGCC 3301 TCTTGCCACC CGTTGAATTT TTGGTGCGAA GACGTGCCTG GGAACGACCT CCACTTTAAA 3361 TTTCTCCGTT GTTGCGGTCG CTCCATCCTC CTCACATTTC TTGCCTCCTA AGTCCCATGT 3421 GCCACACTCG GTGAAAAACG GAGTTTGATA CGGATGAAAT TTCCAGTGAG CTTTTCACAT 3481 TCTGGAGGTT TCGAAAAAAA TTGTGGGATT TTCTGAATTA ATCCGAGAAT CCGCCCTGCC 3541 CATCAACCGG AAAGAATTAA ATCCCTCGCA CATTCCTTTC TCCCACCACC TTCTTTATCT 3601 CTACATTCTT TAACCAAAAC ACCTAGGTGA GTACCCAAAG TCTTTTGACA CCTTTGAAAA 3661 TCCGCGCCAG TCGTAGGGCA CGTAAAACAA TTCTCGCTGG TGGCTTCTGC AGGTGAAGTC 3721 GGGGCAGAGT GGAGCTGAGC ATGGTCGACC ACCCACATCA TTCTGTCTTG TTTTTGGCGA 3781 TGTCTTCCAT GCTTTTGCCC TCATCTGTTG CGTCGACTGC AGAGTACTGT TTTGCCACTA 3841 CTGAGCTCAA CATGGGCTTG TGTGTGGTGA AGAAAAAAAT TTGGTGGCTC TTGAAAATTT 3901 TCAACCCATC GGATACATGC CCTTCCAACT CACAACGCCC TAACACTTTA TTGTCCTATC 3961 ATTGCCTCTT CGCACATGCT AACGATCTCC ACAGAAAACT TCAAAATGGT CTCCAAGGGC 4021 GAGGAGCTCT TCACCGGCGT CGTCCCCATC CTCGTCGAGC TCGACGGCGA CGTTAACGGC 4081 CACAAGTTCT CCGTCTCCGG CGAGGGCGAG GGCGACGCCA CCTACGGCAA GCTCACCCTC 4141 AAGTTCATCT GCACCACCGG CAAGCTCCCC GTCCCCTGGC CCACCCTCGT CACCACCCTC 4201 TCCTGGGGCG TCCAGTGCTT CGCCCGTTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC 4261 AAGTCCGCCA TGCCCGAGGG CTACGTCCAG GAGCGTACCA TCTTCTTCAA GGACGACGGC 4321 AACTACAAGA CCCGTGCCGA GGTCAAGTTC GAGGGCGACA CCCTCGTCAA CCGTATCGAG 4381 CTCAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTCG GCCACAAGCT CGAGTACAAC 4441 TACTTCTCCG ACAACGTCTA CATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC 4501 TTCAAGATCC GTCACAACAT CGAGGACGGC GGCGTCCAGC TCGCCGACCA CTACCAGCAG 4561 AACACCCCCA TCGGCGACGG CCCCGTCCTC CTCCCCGACA ACCACTACCT CTCCACCCAG 4621 TCCAAGCTCT CCAAGGACCC CAACGAGAAG CGTGACCACA TGGTCCTCCT CGAGTTCGTC 4681 ACCGCCGCCG GCATCACTCT CGGCATGGAC GAGCTCTACA AGTAGAAGGT TGAGCGCGTA 4741 ATAGTGGGTT GGTCTTGTCT CTATCCATTC ATCTGTGAGA CATACTGATT TCAACTTTTT 4801 CATCAGATAA TAAGGATGGT GTGAGATAAG CAAGGCTGGG TTATGAGGTT TGGGACTAGA 4861 TGGTGTTTCG CGTCTAAAGG TGAGTCTTCG TTGGTTCAAT CACATTGATC TGATCGCTGA 4921 TCAATTGTCA GCCTCTGAAT GTGCTCTTGA AGAAAATGGT TGTCTGTAAG AATCCAGAAC 4981 CAAAAACGGT CATTTCAAGT ATGTATCCTC CGTGAAGTTC CTTTAGCTCT TGCTAATATT 5041 GGACTTTAGC GTGCTTTAGC CAAAGACAGG GTCGCGAGGT TAAGAGGATG GGCCAGGAAA 5101 AATCTAAGTT AGATTTGTCA CACAAAGAAA AAGATTGTCT CAGTTTTGGT TTTGTTTGGT 5161 TTGATTTATG CATAGGAAAT ATTTATGATC GAAATCACTC ATTTCCTGCT TCGTAAATAT 5221 CATCTACTAT ATGCTTCGTA AATGTCCATC TCCTACATAT TGTGGATATG ATAAATGCAA 5281 TCCTCTCGTC TACACTCTCG GCTTATGCCT TTGTCTTCTT TGCGCTCCTT TTAGCCTTCT 5341 TTTTATTCCT AGTCTTCAAG CCCATCACTA GTTCTAGAGC GGCCGCCACC GCGGTGGAGC 5401 TCCAGCTTTT GTTCCCTTTA GTGAGGGTTA ATTGCGCGCT TGGCGTAATC ATGGTCATAG 5461 CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC 5521 ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC 5581 TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA 5641 CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG 5701 CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG 5761 TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG 5821 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC 5881 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA 5941 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT 6001 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC 6061 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC 6121 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA 6181 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT 6241 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA 6301 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT 6361 TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT 6421 ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT 6481 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC 6541 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA 6601 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA 6661 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC 6721 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT 6781 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA 6841 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT 6901 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT 6961 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG 7021 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC 7081 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC 7141 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG 7201 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA 7261 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA 7321 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT 7381 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG 7441 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA 7501 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 7561 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTCATCA GATCTGCCCA 7621 TAGACTTCAA ATCATGGAAT TGGACGTTTA TCTTCTTGCT TTGGATATCC ACCCTATCGT 7681 ACAATCTTGG CACTTTGGAT CATACGAGGC ACTTTGCGGA CTAGGGAATG GTTATCATCA 7741 ATTTCTTGGA CCTCTCCATG ACACCTGGCA TCGTCGAGCT ACCACAGCCC CCTGAATTCT 7801 TAAAGGCGAA CCCCCCTTCC TAGGAACCTT GAGCGCTGGA CTGGATGGCA CTCTGAGACC 7861 GCCTATGCTT AAGGACGCGA GCTTACCCTT CCATTGATGG AAGTCGATCA TTTATGATAA 7921 TAGAACACGG CGGAACTTGA CTTTACAGGC CAAGGGAAGC AACAGGAGAA GAAGCTAAGG 7981 TTATAACAGG CTCAGCATAT TTGTATATAC GGTGCCATTC AACGCTGAAC ACTTTGTACT 8041 CACGGTCTGC CCGTTGAATA ACACCATCTG CTGATGATCA TCCCAATTTG TGAGTCTTAT 8101 TTGCCATTGA TCCTGGTGTA TAACCGATAC TGAAGCCCTT TAGGTCTGAT CAGTTATAGA 8161 CGTGGTATAT TCATTCTTCC TAGATTAGCT ATGCCGAAAG AGATGGATAC CTTGATGTGA 8221 CTGGAATGCC ATGAAGCAGG AGATTGTAGC AGCTCAAATT GGAGAAGGAT GTTGCAACCG 8281 TCGGCCGTGA AGGTCTTGTC ATATCTTTGT CTGCCTGGGA GAATATGTAC AAGTGCCCCG 8341 ATGTTACTGA TGGAAAACAT CGTGTGGCGC GCCACGATAC TTGTGTTAAT TAA //