LOCUS       pPEE42                  7485 bp    DNA     circular SYN 08-Feb-2019
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     gene            complement(129..986)
                     /note="bla"
     rep_origin      complement(1137..1804)
                     /note="pUC origin"
     promoter        complement(2024..2145)
                     /note="lac promoter"
     gene            join(2146..>2230,<6781..7026)
                     /note="lacZ [Split]"
     misc_feature    complement(join(2202..>2230,<6781..6833))
                     /note="MCS [Split]"
     CDS             2231..>2746
                     /note="mCitrine [Split]"
     regulatory      2747..3976
                     /note="TEF1 promoter"
     CDS             2747..6264
                     /note="amdS2 cassette"
     CDS             3977..5623
                     /note="amdS"
     regulatory      <5624..6264
                     /note="ADE6 terminator"
     CDS             <6265..6780
                     /note="mCitrine [Split]"
     rep_origin      complement(7159..7465)
                     /note="f1 (-) origin"
BASE COUNT      1867 A      2022 C      1840 G      1756 T         0 OTHER
ORIGIN      ?
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG
     1861 AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG
     1921 AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT
     1981 GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG
     2041 TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT
     2101 TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG
     2161 CCAAGCGCGC AATTAACCCT CACTAAAGGG AACAAAAGCT GGAGCTCCAC CGCGGTGGCG
     2221 GCCGCTCTAG ATGGTCTCCA AGGGCGAGGA GCTCTTCACC GGCGTCGTCC CCATCCTCGT
     2281 CGAGCTCGAC GGCGACGTTA ACGGCCACAA GTTCTCCGTC TCCGGCGAGG GCGAGGGCGA
     2341 CGCCACCTAC GGCAAGCTCA CCCTCAAGTT CATCTGCACC ACCGGCAAGC TCCCCGTCCC
     2401 CTGGCCCACC CTCGTCACCA CCTTCGGCTA CGGCCTCATG TGCTTCGCCC GTTACCCCGA
     2461 CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAGGGCTACG TCCAGGAGCG
     2521 TACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGT GCCGAGGTCA AGTTCGAGGG
     2581 CGACACCCTC GTCAACCGTA TCGAGCTCAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT
     2641 CCTCGGCCAC AAGCTCGAGT ACAACTACAA CTCCCACAAC GTCTACATCA TGGCCGACAA
     2701 GCAGAAGAAC GGCATCAAGG TCAACTTCAA GATCCGTCAC AACATCGCGG CCGCCATTCT
     2761 ATGCTATACG GTACAAGATT GTGGCTACTA TGCAAGCTCC ATAGCGAATG ATCTTACACT
     2821 GACCTTGTCG ACCGTTCTGA ATGATTAACA ATGGTCGCAA GTGTGGTTGA CCCACCTGCG
     2881 GGCGCTTCTT AACTATCTAT CATCGGTATG TCAAGTATCA CTCCCTTTAA ACAAAAGAAT
     2941 AAGAGCATAA TACAGCAGTC TCATCATGGC AACCCTTCGC GCAATGTACA AAAAGCAGCC
     3001 AGAAGCCCCC ACGCACAAGC AATAATGTCG TGTTCTACCC TGCCATGAAC AAGCGTCATG
     3061 GCCATTAGCG AACGCCCCAA TATTCAGCCC CATTCGCCAA GCAAGTTGCC TCGCCCCCTT
     3121 CCCAGAAGAT CATGGGCATC TCGTCTTCTT CTAATTTCGC ATTTTGTGAA GCTCCATTCA
     3181 TGCACGTCGG CTTCTGGACG ACTCTTATCA TATGCATTGG CCTGCTCGGT GTGAGGATGA
     3241 GCCACTCCCA CATGTTTAGA CGGCAGGGGC CTCTTGCCAC CCGTTGAATT TTTGGTGCGA
     3301 AGACGTGCCT GGGAACGACC TCCACTTTAA ATTTCTCCGT TGTTGCGGTC GCTCCATCCT
     3361 CCTCACATTT CTTGCCTCCT AAGTCCCATG TGCCACACTC GGTGAAAAAC GGAGTTTGAT
     3421 ACGGATGAAA TTTCCAGTGA GCTTTTCACA TTCTGGAGGT TTCGAAAAAA ATTGTGGGAT
     3481 TTTCTGAATT AATCCGAGAA TCCGCCCTGC CCATCAACCG GAAAGAATTA AATCCCTCGC
     3541 ACATTCCTTT CTCCCACCAC CTTCTTTATC TCTACATTCT TTAACCAAAA CACCTAGGTG
     3601 AGTACCCAAA GTCTTTTGAC ACCTTTGAAA ATCCGCGCCA GTCGTAGGGC ACGTAAAACA
     3661 ATTCTCGCTG GTGGCTTCTG CAGGTGAAGT CGGGGCAGAG TGGAGCTGAG CATGGTCGAC
     3721 CACCCACATC ATTCTGTCTT GTTTTTGGCG ATGTCTTCCA TGCTTTTGCC CTCATCTGTT
     3781 GCGTCGACTG CAGAGTACTG TTTTGCCACT ACTGAGCTCA ACATGGGCTT GTGTGTGGTG
     3841 AAGAAAAAAA TTTGGTGGCT CTTGAAAATT TTCAACCCAT CGGATACATG CCCTTCCAAC
     3901 TCACAACGCC CTAACACTTT ATTGTCCTAT CATTGCCTCT TCGCACATGC TAACGATCTC
     3961 CACAGAAAAC TTCAAAATGC CTCAATCCTG GGAAGAACTG GCCGCTGATA AGCGCGCCCG
     4021 CCTCGCAAAA ACCATCCCTG ATGAATGGAA AGTCCAGACG CTGCCTGCGG AAGACAGCGT
     4081 TATTGATTTC CCAAAGAAAT CGGGGATCCT TTCAGAGGCC GAACTGAAGA TCACAGAGGC
     4141 CTCCGCTGCA GATCTTGTGT CCAAGCTGGC GGCCGGAGAG TTGACCTCGG TGGAAGTTAC
     4201 GCTAGCATTC TGTAAACGGG CAGCAATCGC CCAGCAGTTA ACAAACTGCG CCCACGAGTT
     4261 CTTCCCTGAC GCCGCTCTCG CGCAGGCAAG GGAACTCGAT GAATACTACG CAAAGCACAA
     4321 GAGACCCGTT GGTCCACTCC ATGGCCTCCC CATCTCTCTC AAAGACCAGC TTCGAGTCAA
     4381 GGGCTACGAA ACATCAATGG GCTACATCTC ATGGCTAAAC AAGTACGACG AAGGGGACTC
     4441 GGTTCTGACA ACCATGCTCC GCAAAGCCGG TGCCGTCTTC TACGTCAAGA CCTCTGTCCC
     4501 GCAGACCCTG ATGGTCTGCG AGACAGTCAA CAACATCATC GGGCGCACCG TCAACCCACG
     4561 CAACAAGAAC TGGTCGTGCG GCGGCAGTTC TGGTGGTGAG GGTGCGATCG TTGGGATTCG
     4621 TGGTGGCGTC ATCGGTGTAG GAACGGATAT CGGTGGCTCG ATTCGAGTGC CGGCCGCGTT
     4681 CAACTTCCTG TACGGTCTAA GGCCGAGTCA TGGGCGGCTG CCGTATGCAA AGATGGCGAA
     4741 CAGCATGGAG GGTCAGGAGA CGGTGCACAG CGTTGTCGGG CCGATTACGC ACTCTGTTGA
     4801 GGACCTCCGC CTCTTCACCA AATCCGTCCT CGGTCAGGAG CCATGGAAAT ACGACTCCAA
     4861 GGTCATCCCC ATGCCCTGGC GCCAGTCCGA GTCGGACATT ATTGCCTCCA AGATCAAGAA
     4921 CGGCGGGCTC AATATCGGCT ACTACAACTT CGACGGCAAT GTCCTTCCAC ACCCTCCTAT
     4981 CCTGCGCGGC GTGGAAACCA CCGTCGCCGC ACTCGCCAAA GCCGGTCACA CCGTGACCCC
     5041 GTGGACGCCA TACAAGCACG ATTTCGGCCA CGATCTCATC TCCCATATCT ACGCGGCTGA
     5101 CGGCAGCGCC GACGTAATGC GCGATATCAG TGCATCCGGC GAGCCGGCGA TTCCAAATAT
     5161 CAAAGACCTA CTGAACCCGA ACATCAAAGC TGTTAACATG AACGAGCTCT GGGACACGCA
     5221 TCTCCAGAAG TGGAATTACC AGATGGAGTA CCTTGAGAAA TGGCGGGAGG CTGAAGAAAA
     5281 GGCCGGGAAG GAACTGGACG CCATCATCGC GCCGATTACG CCTACCGCTG CGGTACGGCA
     5341 TGACCAGTTC CGGTACTATG GGTATGCCTC TGTGATCAAC CTGCTGGATT TCACGAGCGT
     5401 GGTTGTTCCG GTTACCTTTG CGGATAAGAA CATCGATAAG AAGAATGAGA GTTTCAAGGC
     5461 GGTTAGTGAG CTTGATGCCC TCGTGCAGGA AGAGTATGAT CCGGAGGCGT ACCATGGGGC
     5521 ACCGGTTGCA GTGCAGGTTA TCGGACGGAG ACTCAGTGAA GAGAGGACGT TGGCGATTGC
     5581 AGAGGAAGTG GGGAAGTTGC TGGGAAATGT GGTGACTCCA TAGAAGGTTG AGCGCGTAAT
     5641 AGTGGGTTGG TCTTGTCTCT ATCCATTCAT CTGTGAGACA TACTGATTTC AACTTTTTCA
     5701 TCAGATAATA AGGATGGTGT GAGATAAGCA AGGCTGGGTT ATGAGGTTTG GGACTAGATG
     5761 GTGTTTCGCG TCTAAAGGTG AGTCTTCGTT GGTTCAATCA CATTGATCTG ATCGCTGATC
     5821 AATTGTCAGC CTCTGAATGT GCTCTTGAAG AAAATGGTTG TCTGTAAGAA TCCAGAACCA
     5881 AAAACGGTCA TTTCAAGTAT GTATCCTCCG TGAAGTTCCT TTAGCTCTTG CTAATATTGG
     5941 ACTTTAGCGT GCTTTAGCCA AAGACAGGGT CGCGAGGTTA AGAGGATGGG CCAGGAAAAA
     6001 TCTAAGTTAG ATTTGTCACA CAAAGAAAAA GATTGTCTCA GTTTTGGTTT TGTTTGGTTT
     6061 GATTTATGCA TAGGAAATAT TTATGATCGA AATCACTCAT TTCCTGCTTC GTAAATATCA
     6121 TCTACTATAT GCTTCGTAAA TGTCCATCTC CTACATATTG TGGATATGAT AAATGCAATC
     6181 CTCTCGTCTA CACTCTCGGC TTATGCCTTT GTCTTCTTTG CGCTCCTTTT AGCCTTCTTT
     6241 TTATTCCTAG TCTTCAAGCC CATCGGCCTC ATGTGCTTCG CCCGTTACCC CGACCACATG
     6301 AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAGGGCT ACGTCCAGGA GCGTACCATC
     6361 TTCTTCAAGG ACGACGGCAA CTACAAGACC CGTGCCGAGG TCAAGTTCGA GGGCGACACC
     6421 CTCGTCAACC GTATCGAGCT CAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTCGGC
     6481 CACAAGCTCG AGTACAACTA CAACTCCCAC AACGTCTACA TCATGGCCGA CAAGCAGAAG
     6541 AACGGCATCA AGGTCAACTT CAAGATCCGT CACAACATCG AGGACGGCTC CGTCCAGCTC
     6601 GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTCCTCCT CCCCGACAAC
     6661 CACTACCTCT CCTACCAGTC CGCCCTCTCC AAGGACCCCA ACGAGAAGCG TGACCACATG
     6721 GTCCTCCTCG AGTTCGTCAC CGCCGCCGGC ATCACTCTCG GCATGGACGA GCTTTACAAG
     6781 AATTCGATAT CAAGCTTATC GATACCGTCG ACCTCGAGGG GGGGCCCGGT ACCCAATTCG
     6841 CCCTATAGTG AGTCGTATTA CGCGCGCTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG
     6901 GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG
     6961 CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC
     7021 GAATGGAAAT TGTAAGCGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA
     7081 GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA
     7141 CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG
     7201 ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT
     7261 CACCCTAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG
     7321 GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA
     7381 AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA
     7441 CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTCAG
//
