LOCUS       Exported                6536 bp ds-DNA     circular SYN 22-Jan-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    p_SNIPR_sensor_LacZ
SOURCE      synthetic DNA construct
ORGANISM    synthetic DNA construct
REFERENCE   1  (bases 1 to 4142)
  AUTHORS     ALEXANDER A. GREEN
FEATURES             Location/Qualifiers
     promoter        6520..2
                     /note="T7 promoter"
     promoter        complement(6383..6460)
                     /note="lacI promoter"
     CDS             127..3201
                     /note="lacZ"
     terminator      3266..3312
                     /note="T7 terminator"
     CDS             complement(3542..4357)
                     /note="KanR"
     promoter        complement(4358..4449)
                     /note="AmpR promoter"
     rep_origin      complement(4467..5102)
                     /note="ColA ori"
     CDS             complement(5300..6382)
                     /note="lacI"
     misc_feature    1..117
                     /note="SNIPR"
ORIGIN
         1 GGGTAGAATG AAATGAATAA GACAATAACA GTAACATGGT AGGTAAGTGT GATAACATCA
        61 TAGAGGAGAT ACAGAATGTT ATCACACTTA CCTACCGAAC AAGAACTCCG ACAAATCATG
       121 CGTAAAATGA CCATGATTAC GGATTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA
       181 AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT
       241 AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA
       301 TGGCGCTTTG CCTGGTTTCC GGCACCAGAA GCGGTGCCGG AAAGCTGGCT GGAGTGCGAT
       361 CTTCCTGAGG CCGATACTGT CGTCGTCCCC TCAAACTGGC AGATGCACGG TTACGATGCG
       421 CCCATCTACA CCAACGTGAC CTATCCCATT ACGGTCAATC CGCCGTTTGT TCCCACGGAG
       481 AATCCGACGG GTTGTTACTC GCTCACATTT AATGTTGATG AAAGCTGGCT ACAGGAAGGC
       541 CAGACGCGAA TTATTTTTGA TGGCGTTAAC TCGGCGTTTC ATCTGTGGTG CAACGGGCGC
       601 TGGGTCGGTT ACGGCCAGGA CAGTCGTTTG CCGTCTGAAT TTGACCTGAG CGCATTTTTA
       661 CGCGCCGGAG AAAACCGCCT CGCGGTGATG GTGCTGCGCT GGAGTGACGG CAGTTATCTG
       721 GAAGATCAGG ATATGTGGCG GATGAGCGGC ATTTTCCGTG ACGTCTCGTT GCTGCATAAA
       781 CCGACTACAC AAATCAGCGA TTTCCATGTT GCCACTCGCT TTAATGATGA TTTCAGCCGC
       841 GCTGTACTGG AGGCTGAAGT TCAGATGTGC GGCGAGTTGC GTGACTACCT ACGGGTAACA
       901 GTTTCTTTAT GGCAGGGTGA AACGCAGGTC GCCAGCGGCA CCGCGCCTTT CGGCGGTGAA
       961 ATTATCGATG AGCGTGGTGG TTATGCCGAT CGCGTCACAC TACGTCTGAA CGTCGAAAAC
      1021 CCGAAACTGT GGAGCGCCGA AATCCCGAAT CTCTATCGTG CGGTGGTTGA ACTGCACACC
      1081 GCCGACGGCA CGCTGATTGA AGCAGAAGCC TGCGATGTCG GTTTCCGCGA GGTGCGGATT
      1141 GAAAATGGTC TGCTGCTGCT GAACGGCAAG CCGTTGCTGA TTCGAGGCGT TAACCGTCAC
      1201 GAGCATCATC CTCTGCATGG TCAGGTCATG GATGAGCAGA CGATGGTGCA GGATATCCTG
      1261 CTGATGAAGC AGAACAACTT TAACGCCGTG CGCTGTTCGC ATTATCCGAA CCATCCGCTG
      1321 TGGTACACGC TGTGCGACCG CTACGGCCTG TATGTGGTGG ATGAAGCCAA TATTGAAACC
      1381 CACGGCATGG TGCCAATGAA TCGTCTGACC GATGATCCGC GCTGGCTACC GGCGATGAGC
      1441 GAACGCGTAA CGCGAATGGT GCAGCGCGAT CGTAATCACC CGAGTGTGAT CATCTGGTCG
      1501 CTGGGGAATG AATCAGGCCA CGGCGCTAAT CACGACGCGC TGTATCGCTG GATCAAATCT
      1561 GTCGATCCTT CCCGCCCGGT GCAGTATGAA GGCGGCGGAG CCGACACCAC GGCCACCGAT
      1621 ATTATTTGCC CGATGTACGC GCGCGTGGAT GAAGACCAGC CCTTCCCGGC TGTGCCGAAA
      1681 TGGTCCATCA AAAAATGGCT TTCGCTACCT GGAGAGACGC GCCCGCTGAT CCTTTGCGAA
      1741 TACGCCCACG CGATGGGTAA CAGTCTTGGC GGTTTCGCTA AATACTGGCA GGCGTTTCGT
      1801 CAGTATCCCC GTTTACAGGG CGGCTTCGTC TGGGACTGGG TGGATCAGTC GCTGATTAAA
      1861 TATGATGAAA ACGGCAACCC GTGGTCGGCT TACGGCGGTG ATTTTGGCGA TACGCCGAAC
      1921 GATCGCCAGT TCTGTATGAA CGGTCTGGTC TTTGCCGACC GCACGCCGCA TCCAGCGCTG
      1981 ACGGAAGCAA AACACCAGCA GCAGTTTTTC CAGTTCCGTT TATCCGGGCA AACCATCGAA
      2041 GTGACCAGCG AATACCTGTT CCGTCATAGC GATAACGAGC TCCTGCACTG GATGGTGGCG
      2101 CTGGATGGTA AGCCGCTGGC AAGCGGTGAA GTGCCTCTGG ATGTCGCTCC ACAAGGTAAA
      2161 CAGTTGATTG AACTGCCTGA ACTACCGCAG CCGGAGAGCG CCGGGCAACT CTGGCTCACA
      2221 GTACGCGTAG TGCAACCGAA CGCGACCGCA TGGTCAGAAG CCGGGCACAT CAGCGCCTGG
      2281 CAGCAGTGGC GTCTGGCGGA AAACCTCAGT GTGACGCTCC CCGCCGCGTC CCACGCCATC
      2341 CCGCATCTGA CCACCAGCGA AATGGATTTT TGCATCGAGC TGGGTAATAA GCGTTGGCAA
      2401 TTTAACCGCC AGTCAGGCTT TCTTTCACAG ATGTGGATTG GCGATAAAAA ACAACTGCTG
      2461 ACGCCGCTGC GCGATCAGTT CACCCGTGCA CCGCTGGATA ACGACATTGG CGTAAGTGAA
      2521 GCGACCCGCA TTGACCCTAA CGCCTGGGTC GAACGCTGGA AGGCGGCGGG CCATTACCAG
      2581 GCCGAAGCAG CGTTGTTGCA GTGCACGGCA GATACACTTG CTGATGCGGT GCTGATTACG
      2641 ACCGCTCACG CGTGGCAGCA TCAGGGGAAA ACCTTATTTA TCAGCCGGAA AACCTACCGG
      2701 ATTGATGGTA GTGGTCAAAT GGCGATTACC GTTGATGTTG AAGTGGCGAG CGATACACCG
      2761 CATCCGGCGC GGATTGGCCT GAACTGCCAG CTGGCGCAGG TAGCAGAGCG GGTAAACTGG
      2821 CTCGGATTAG GGCCGCAAGA AAACTATCCC GACCGCCTTA CTGCCGCCTG TTTTGACCGC
      2881 TGGGATCTGC CATTGTCAGA CATGTATACC CCGTACGTCT TCCCGAGCGA AAACGGTCTG
      2941 CGCTGCGGGA CGCGCGAATT GAATTATGGC CCACACCAGT GGCGCGGCGA CTTCCAGTTC
      3001 AACATCAGCC GCTACAGTCA ACAGCAACTG ATGGAAACCA GCCATCGCCA TCTGCTGCAC
      3061 GCGGAAGAAG GCACATGGCT GAATATCGAC GGTTTCCATA TGGGGATTGG TGGCGACGAC
      3121 TCCTGGAGCC CGTCAGTATC GGCGGAATTC CAGCTGAGCG CCGGTCGCTA CCATTACCAG
      3181 TTGGTCTGGT GTCAAAAATA AGATAAACCA GAGCGGCACG GCAAGCAGAG TATACGAGAT
      3241 TCGGTAGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG
      3301 AGGGGTTTTT TGCTGAAACC TCAGGCATTT GAGAAGCACA CGGTCACACT GCTTCCGGTA
      3361 GTCAATAAAC CGGTAAACCA GCAATAGACA TAAGCGGCTA TTTAACGACC CTGCCCTGAA
      3421 CCGACGACAA GCTGACGACC GGGTCTCCGC AAGTGGCACT TTTCGGGGAA ATGTGCGCGG
      3481 AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAATTAATT
      3541 CTTAGAAAAA CTCATCGAGC ATCAAATGAA ACTGCAATTT ATTCATATCA GGATTATCAA
      3601 TACCATATTT TTGAAAAAGC CGTTTCTGTA ATGAAGGAGA AAACTCACCG AGGCAGTTCC
      3661 ATAGGATGGC AAGATCCTGG TATCGGTCTG CGATTCCGAC TCGTCCAACA TCAATACAAC
      3721 CTATTAATTT CCCCTCGTCA AAAATAAGGT TATCAAGTGA GAAATCACCA TGAGTGACGA
      3781 CTGAATCCGG TGAGAATGGC AAAAGTTTAT GCATTTCTTT CCAGACTTGT TCAACAGGCC
      3841 AGCCATTACG CTCGTCATCA AAATCACTCG CATCAACCAA ACCGTTATTC ATTCGTGATT
      3901 GCGCCTGAGC GAGACGAAAT ACGCGGTCGC TGTTAAAAGG ACAATTACAA ACAGGAATCG
      3961 AATGCAACCG GCGCAGGAAC ACTGCCAGCG CATCAACAAT ATTTTCACCT GAATCAGGAT
      4021 ATTCTTCTAA TACCTGGAAT GCTGTTTTCC CGGGGATCGC AGTGGTGAGT AACCATGCAT
      4081 CATCAGGAGT ACGGATAAAA TGCTTGATGG TCGGAAGAGG CATAAATTCC GTCAGCCAGT
      4141 TTAGTCTGAC CATCTCATCT GTAACATCAT TGGCAACGCT ACCTTTGCCA TGTTTCAGAA
      4201 ACAACTCTGG CGCATCGGGC TTCCCATACA ATCGATAGAT TGTCGCACCT GATTGCCCGA
      4261 CATTATCGCG AGCCCATTTA TACCCATATA AATCAGCATC CATGTTGGAA TTTAATCGCG
      4321 GCCTAGAGCA AGACGTTTCC CGTTGAATAT GGCTCATACT CTTCCTTTTT CAATATTATT
      4381 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA
      4441 ATAAACAAAT AGGCATGCTA GCGCAGAAAC GTCCTAGAAG ATGCCAGGAG GATACTTAGC
      4501 AGAGAGACAA TAAGGCCGGA GCGAAGCCGT TTTTCCATAG GCTCCGCCCC CCTGACGAAC
      4561 ATCACGAAAT CTGACGCTCA AATCAGTGGT GGCGAAACCC GACAGGACTA TAAAGATACC
      4621 AGGCGTTTCC CCCTGATGGC TCCCTCTTGC GCTCTCCTGT TCCCGTCCTG CGGCGTCCGT
      4681 GTTGTGGTGG AGGCTTTACC CAAATCACCA CGTCCCGTTC CGTGTAGACA GTTCGCTCCA
      4741 AGCTGGGCTG TGTGCAAGAA CCCCCCGTTC AGCCCGACTG CTGCGCCTTA TCCGGTAACT
      4801 ATCATCTTGA GTCCAACCCG GAAAGACACG ACAAAACGCC ACTGGCAGCA GCCATTGGTA
      4861 ACTGAGAATT AGTGGATTTA GATATCGAGA GTCTTGAAGT GGTGGCCTAA CAGAGGCTAC
      4921 ACTGAAAGGA CAGTATTTGG TATCTGCGCT CCACTAAAGC CAGTTACCAG GTTAAGCAGT
      4981 TCCCCAACTG ACTTAACCTT CGATCAAACC GCCTCCCCAG GCGGTTTTTT CGTTTACAGA
      5041 GCAGGAGATT ACGACGATCG TAAAAGGATC TCAAGAAGAT CCTTTACGGA TTCCCGACAC
      5101 CATCACTCTA GATTTCAGTG CAATTTATCT CTTCAAATGT AGCACCTGAA GTCAGCCCCA
      5161 TACGATATAA GTTGTAATTC TCATGTTAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC
      5221 TGGGTTGAAG GCTCTCAAGG GCATCGGTCG AGATCCCGGT GCCTAATGAG TGAGCTAACT
      5281 TACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT
      5341 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCCAGGGTGG
      5401 TTTTTCTTTT CACCAGTGAG ACGGGCAACA GCTGATTGCC CTTCACCGCC TGGCCCTGAG
      5461 AGAGTTGCAG CAAGCGGTCC ACGCTGGTTT GCCCCAGCAG GCGAAAATCC TGTTTGATGG
      5521 TGGTTAACGG CGGGATATAA CATGAGCTGT CTTCGGTATC GTCGTATCCC ACTACCGAGA
      5581 TGTCCGCACC AACGCGCAGC CCGGACTCGG TAATGGCGCG CATTGCGCCC AGCGCCATCT
      5641 GATCGTTGGC AACCAGCATC GCAGTGGGAA CGATGCCCTC ATTCAGCATT TGCATGGTTT
      5701 GTTGAAAACC GGACATGGCA CTCCAGTCGC CTTCCCGTTC CGCTATCGGC TGAATTTGAT
      5761 TGCGAGTGAG ATATTTATGC CAGCCAGCCA GACGCAGACG CGCCGAGACA GAACTTAATG
      5821 GGCCCGCTAA CAGCGCGATT TGCTGGTGAC CCAATGCGAC CAGATGCTCC ACGCCCAGTC
      5881 GCGTACCGTC TTCATGGGAG AAAATAATAC TGTTGATGGG TGTCTGGTCA GAGACATCAA
      5941 GAAATAACGC CGGAACATTA GTGCAGGCAG CTTCCACAGC AATGGCATCC TGGTCATCCA
      6001 GCGGATAGTT AATGATCAGC CCACTGACGC GTTGCGCGAG AAGATTGTGC ACCGCCGCTT
      6061 TACAGGCTTC GACGCCGCTT CGTTCTACCA TCGACACCAC CACGCTGGCA CCCAGTTGAT
      6121 CGGCGCGAGA TTTAATCGCC GCGACAATTT GCGACGGCGC GTGCAGGGCC AGACTGGAGG
      6181 TGGCAACGCC AATCAGCAAC GACTGTTTGC CCGCCAGTTG TTGTGCCACG CGGTTGGGAA
      6241 TGTAATTCAG CTCCGCCATC GCCGCTTCCA CTTTTTCCCG CGTTTTCGCA GAAACGTGGC
      6301 TGGCCTGGTT CACCACGCGG GAAACGGTCT GATAAGAGAC ACCGGCATAC TCTGCGACAT
      6361 CGTATAACGT TACTGGTTTC ACATTCACCA CCCTGAATTG ACTCTCTTCC GGGCGCTATC
      6421 ATGCCATACC GCGAAAGGTT TTGCGCCATT CGATGGTGTC CGGGATCTCG ACGCTCTCCC
      6481 TTATGCTAGT AAATTCGCGT TTCTACGGTA GCCGGGCGCT AATACGACTC ACTATA
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