LOCUS       Exported                8139 bp ds-DNA     circular SYN 03-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 12, 2020 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Ligation of : pSP264_pSTL1_PP7-SL.gb [7443  nt] : (BamHI[2508] / 
            SacI[1690]) to pVW288_pRS305_pALD3_UbiY_2xNLS_SZ3.xdna [695  nt] : 
            (SacI[34] / BamHI[733])
FEATURES             Location/Qualifiers
     source          1..8139
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    9..367
                     /label=pGLT1 -550 : -200
     misc_feature    760..1458
                     /label=S. Klu his3
     misc_feature    1646..1665
                     /label=4242
     misc_feature    1696..2369
                     /label=pALD3
     misc_feature    2383..3712
                     /label=24xPP7 SL
     misc_feature    3873..4177
                     /label=Glt1 +1: 465
     primer_bind     complement(4214..4233)
                     /label=T7
     CDS             4484..4693
                     /label=ccdB
     promoter        4907..4956
                     /label=/label
     CDS             5048..5839
                     /label=Kan/neoR
     gene            complement(6089..6947)
                     /label=Ampicillin
     rep_origin      7049..7677
                     /direction=RIGHT
                     /label=ColE1 origin
     promoter        7983..8012
                     /label=lac
     misc_binding    8017..8039
                     /label=LacO
     primer_bind     8045..8065
                     /label=M13-rev
     primer_bind     8083..8102
                     /label=T3
ORIGIN
        1 ttaattaaac cgttcaaccc tatacagcgc aaatttggtc ctaatacact tcggttttaa
       61 tgcgtcaatc cgattggctc cgataagctt ttgcacattt ttcaagtcat atgtcacgac
      121 gaacgtgatg cccgcaaaac gtaaaaaaaa gaagcaataa attgcccaaa aaaaaaatat
      181 aaactgcttt tcttagtcat tcataagttc tgatggtctt ccttatctta ttgtattttt
      241 cgtcctattg tttcatttct taccaaatta atcaattctt atatcttact tgataacaca
      301 ccaaactaat cgtctccaca tcataggaag ataggaaatt gctatctcag tcctatacta
      361 cgcagacctc gagtctgttt agcttgcctc gtccccgccg ggtcacccgg ccagcgacat
      421 ggaggcccag aataccctcc ttgacagtct tgacgtgcgc agctcagggg catgatgtga
      481 ctgtcgcccg tacatttagc ccatacatcc ccatgtataa tcatttgcat ccatacattt
      541 tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg
      601 aaacgctccc ctcacagacg cgttgaattg tccccacgcc gcgcccctgt agagaaatat
      661 aaaaggttag gatttgccac tgaggttctt ctttcatata cttcctttta aaatcttgct
      721 aggatacagt tctcacatca catccgaaca taaacaacca tggcagaacc agcccaaaaa
      781 aagcaaaaac aaactgttca ggagcgcaag gcgtttatct cccgtatcac taatgaaact
      841 aaaattcaaa tcgctatttc gctgaatggt ggttatattc aaataaaaga ttcgattctt
      901 cctgcaaaga aggatgacga tgtagcttcc caagctactc agtcacaggt catcgatatt
      961 cacacaggtg ttggcttttt ggatcatatg atccatgcgt tggcaaaaca ctctggttgg
     1021 tctcttattg ttgaatgtat tggtgacctg cacattgacg atcaccatac taccgaagat
     1081 tgcggtatcg cattagggca agcgttcaaa gaagcaatgg gtgctgtccg tggtgtaaaa
     1141 agattcggta ctgggttcgc accattggat gaggcgctat cacgtgccgt agtcgattta
     1201 tctagtagac catttgctgt aatcgacctt ggattgaaga gagagatgat tggtgattta
     1261 tccactgaaa tgattccaca ctttttggaa agtttcgcgg aggcggccag aattactttg
     1321 catgttgatt gtctgagagg tttcaacgat caccacagaa gtgagagtgc gttcaaggct
     1381 ttggctgttg ccataagaga agctatttct agcaatggca ccaatgacgt tccctcaacc
     1441 aaaggtgttt tgatgtgaag tactgacaat aaaaagattc ttgttttcaa gaacttgtca
     1501 tttgtatagt ttttttatat tgtagttgtt ctattttaat caaatgttag cgtgatttat
     1561 attttttttc gcctcgacat catctgccca gatgcgaagt taagtgcgca gaaagtaata
     1621 tcatgcgtca atcgtatgtg aatgctggtc gctatactgc tgtcgattcg atactaacgc
     1681 cgccatccag agctcttatg taagtgcgta agtacctaaa tagcttagcc tgacgacgga
     1741 tgaatatgtt gacagtctag caaacagtag tagtccacta ctcgatataa aactgtaagc
     1801 accattattt cagcatgcgg aacaattgtt gttactaggt tctagaaatc ccttaagatg
     1861 gtaaaaaaat agagctataa actcagtgag atttattttt gtgtcaagaa attatgcttg
     1921 cgtgttagtt ttctctattc ccaccattac gtagatctcc cgtctttttt ttttcttttt
     1981 ctgcatatga cgtctgttct tccttccccc cctggagtac aaggaaagta tcactattag
     2041 aactctacag tacctttctt ccacgccctt tagtgcattt agggattttc attactgcta
     2101 atccaagact tgaaccggat tgttgatcag cccccttctt aagctgattg cgaaactccc
     2161 taagggttaa ggaattagta agtttaggga ggagacaggg agattgttca aacgaatagc
     2221 gtagatttaa ctgatcatac ctcagaaact aattgtataa aatggcaaat ctggatggga
     2281 ataaatttga ctcatgccag gtaattatac cttggacttg tatcatagag atatagatta
     2341 tacacattta gaaaatagcc aaaagaaaat ctagaactag tggatcctaa ggtacctaat
     2401 tgcctagaaa ggagcagacg atatggcgtc gctccctgca ggtcgactct agaaaccagc
     2461 agagcatatg ggctcgctgg ctgcagtatt cccgggttca ttagatccta aggtacctaa
     2521 ttgcctagaa aggagcagac gatatggcgt cgctccctgc aggtcgactc tagaaaccag
     2581 cagagcatat gggctcgctg gctgcagtat tcccgggttc attagatcct aaggtaccta
     2641 attgcctaga aaggagcaga cgatatggcg tcgctccctg caggtcgact ctagaaacca
     2701 gcagagcata tgggctcgct ggctgcagta ttcccgggtt cattagatcc taaggtacct
     2761 aattgcctag aaaggagcag acgatatggc gtcgctccct gcaggtcgac tctagaaacc
     2821 agcagagcat atgggctcgc tggctgcagt attcccgggt tcattagatc ctaaggtacc
     2881 taattgccta gaaaggagca gacgatatgg cgtcgctccc tgcaggtcga ctctagaaac
     2941 cagcagagca tatgggctcg ctggctgcag tattcccggg ttcattagat cctaaggtac
     3001 ctaattgcct agaaaggagc agacgatatg gcgtcgctcc ctgcaggtcg actctagaaa
     3061 ccagcagagc atatgggctc gctggctgca gtattcccgg gttcattaga tcctaaggta
     3121 cctaattgcc tagaaaggag cagacgatat ggcgtcgctc cctgcaggtc gactctagaa
     3181 accagcagag catatgggct cgctggctgc agtattcccg ggttcattag atcctaaggt
     3241 acctaattgc ctagaaagga gcagacgata tggcgtcgct ccctgcaggt cgactctaga
     3301 aaccagcaga gcatatgggc tcgctggctg cagtattccc gggttcatta gatcctaagg
     3361 tacctaattg cctagaaagg agcagacgat atggcgtcgc tccctgcagg tcgactctag
     3421 aaaccagcag agcatatggg ctcgctggct gcagtattcc cgggttcatt agatcctaag
     3481 gtacctaatt gcctagaaag gagcagacga tatggcgtcg ctccctgcag gtcgactcta
     3541 gaaaccagca gagcatatgg gctcgctggc tgcagtattc ccgggttcat tagatcctaa
     3601 ggtacctaat tgcctagaaa ggagcagacg atatggcgtc gctccctgca ggtcgactct
     3661 agaaaccagc agagcatatg ggctcgctgg ctgcagtatt cccgggttca ttagatccta
     3721 aggtacctaa ttgcctagaa aggagcagac gatatggcgt cgctccctgc aggtcgactc
     3781 tagaaaccag cagagcatat gggctcgctg gctgcagtat tcccgggttc attagatcca
     3841 tgtcccagga tagaattttg ttagatagat ctatgccagt gttgaaatca gacaatttcg
     3901 atccattgga agaagcttac gaaggtggga caattcaaaa ctataacgat gaacaccatc
     3961 ttcataaatc ttgggcaaat gtgattccgg acaaacgagg actttacgac cctgattatg
     4021 aacatgacgc ttgtggtgtc ggtttcgtag caaataagca tggtgaacag tctcacaaga
     4081 ttgttactga cgctagatat cttttagtga atatgacaca tcgtggtgcc gtctcatctg
     4141 atgggaacgg tgacggtgcc ggtattctgc taggtatccc aagggcgaat tcgcggccgc
     4201 taaattcaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg
     4261 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     4321 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     4381 cctatacgta cggcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt
     4441 tgtggatgta cagagtgata ttattgacac gccggggcga cggatggtga tccccctggc
     4501 cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg
     4561 ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg
     4621 ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat
     4681 gttctgggga atataaatgt caggcatgag attatcaaaa aggatcttca cctagatcct
     4741 tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg tcagctactg
     4801 ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt gcagtgggct
     4861 tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg aattgccagc
     4921 tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc
     4981 gccaaggatc tgatggcgca ggggatcaag ctctgatcaa gagacaggat gaggatcgtt
     5041 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     5101 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     5161 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     5221 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     5281 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     5341 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     5401 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     5461 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     5521 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
     5581 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     5641 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     5701 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     5761 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     5821 tcttgacgag ttcttctgaa ttattaacgc ttacaatttc ctgatgcggt attttctcct
     5881 tacgcatctg tgcggtattt cacaccgcat caggtggcac ttttcgggga aatgtgcgcg
     5941 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagattat
     6001 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6061 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6121 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6181 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gaccacgctc
     6241 accggctcca gatttatcag caataaacca gccagccgga aggccgagcg cagaagtggt
     6301 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     6361 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     6421 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     6481 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     6541 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     6601 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     6661 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     6721 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     6781 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     6841 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     6901 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     6961 caatattatt gaagcattta tcagggttat tgtctcatga ccaaaatccc ttaacgtgag
     7021 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     7081 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7141 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7201 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     7261 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7321 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7381 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7441 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7501 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7561 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7621 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7681 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7741 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7801 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     7861 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     7921 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     7981 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     8041 cacacaggaa acagctatga ccatgattac gccaagctca gaattaaccc tcactaaagg
     8101 gactagtcct gcaggtttaa acgaattcgc ccttatata
//
