LOCUS       Exported                8076 bp ds-DNA     circular SYN 05-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   serge
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 12, 2020 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8076
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    9..367
                     /label=pGLT1 -550 : -200
     promoter        416..759
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     misc_feature    760..1458
                     /label=S. Klu his3
     terminator      1462..1659
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     misc_feature    1646..1665
                     /label=4242
     misc_feature    1695..2508
                     /label=STL1 promoter
                     /note="note=""Fragment extracted from 
                     pRS303_Pstl1_qVenus.xdna [814  nt] : (SacI[2073] / 
                     BamHI[2891])"
     primer_bind     2277..2296
                     /label=160
     misc_feature    2514..3803
                     /label=24xMS2 SL
     misc_feature    3810..4114
                     /label=Glt1 +1: 465
     primer_bind     complement(4151..4170)
                     /label=T7
     primer_bind     complement(4152..4170)
                     /label=T7
     misc_feature    4174..4317
                     /label=lacZ_a
                     /note="/label=""lacZ_a"" - 
                     /translation=""MGSLAAVFPGSLDPKVPNCLERSRRYGVAPCRSTLETSRAYGLA
                      - 
                     /GCSIPGFIRSMSQDRILLDRSMPVLKSDNFDPLEEAYEGGTIQNYNDEHHLHKSWANV
                      - 
                     /IPDKRGLYDPDYEHDACGVGFVANKHGEQSHKIVTDARYLLVNMTHRGAVSSDGNGDG
                      - /AGILLGIPRANSRPLNSIRPIVSRITIHWPSFYNVVTGKTLALPNLIALQH"
     primer_bind     complement(4177..4194)
                     /label=M13-fwd
     CDS             4331..4633
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     promoter        4844..4893
                     /label=NEOKAN_promoter
                     /note="/label=""NEOKAN_promoter"" - 
                     /translation=""MGSLAAVFPGSLDPKVPNCLERSRRYGVAPCRSTLETSRAYGLA
                      - 
                     /GCSIPGFIRSMSQDRILLDRSMPVLKSDNFDPLEEAYEGGTIQNYNDEHHLHKSWANV
                      - 
                     /IPDKRGLYDPDYEHDACGVGFVANKHGEQSHKIVTDARYLLVNMTHRGAVSSDGNGDG
                      - /AGILLGIPRANSRPLNSIRPIVSRITIHWPSFYNVVTGKTLA"
     CDS             4985..5776
                     /label=Kan/neoR
     gene            complement(6026..6884)
                     /label=Ampicillin_Ampicillin
                     /note="/label=""Ampicillin"" - /gene=""Ampicillin"" - 
                     /translation=""MRKEKIPHQEIVSVNNSEELVKKAIEGDALRIGSGDTVKHEEAV
                      - 
                     /SPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILAV
                      - 
                     /GHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQP
                      - /PHCISHDGYFLGRSKVR*"" - 
com"
     rep_origin      6986..7614
                     /direction=RIGHT
                     /label=ColE1 origin
     protein_bind    7884..7905
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7920..7950
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     misc_binding    7954..7976
                     /label=LacO
     primer_bind     7982..8002
                     /label=M13-rev
     promoter        8019..8038
                     /label=T3_promoter
                     /note="/label=""T3_promoter"" - 
                     /translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                      - 
                     /IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                      - 
                     /YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                      - /DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLI"
ORIGIN
        1 ttaattaaac cgttcaaccc tatacagcgc aaatttggtc ctaatacact tcggttttaa
       61 tgcgtcaatc cgattggctc cgataagctt ttgcacattt ttcaagtcat atgtcacgac
      121 gaacgtgatg cccgcaaaac gtaaaaaaaa gaagcaataa attgcccaaa aaaaaaatat
      181 aaactgcttt tcttagtcat tcataagttc tgatggtctt ccttatctta ttgtattttt
      241 cgtcctattg tttcatttct taccaaatta atcaattctt atatcttact tgataacaca
      301 ccaaactaat cgtctccaca tcataggaag ataggaaatt gctatctcag tcctatacta
      361 cgcagacctc gagtctgttt agcttgcctc gtccccgccg ggtcacccgg ccagcgacat
      421 ggaggcccag aataccctcc ttgacagtct tgacgtgcgc agctcagggg catgatgtga
      481 ctgtcgcccg tacatttagc ccatacatcc ccatgtataa tcatttgcat ccatacattt
      541 tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg
      601 aaacgctccc ctcacagacg cgttgaattg tccccacgcc gcgcccctgt agagaaatat
      661 aaaaggttag gatttgccac tgaggttctt ctttcatata cttcctttta aaatcttgct
      721 aggatacagt tctcacatca catccgaaca taaacaacca tggcagaacc agcccaaaaa
      781 aagcaaaaac aaactgttca ggagcgcaag gcgtttatct cccgtatcac taatgaaact
      841 aaaattcaaa tcgctatttc gctgaatggt ggttatattc aaataaaaga ttcgattctt
      901 cctgcaaaga aggatgacga tgtagcttcc caagctactc agtcacaggt catcgatatt
      961 cacacaggtg ttggcttttt ggatcatatg atccatgcgt tggcaaaaca ctctggttgg
     1021 tctcttattg ttgaatgtat tggtgacctg cacattgacg atcaccatac taccgaagat
     1081 tgcggtatcg cattagggca agcgttcaaa gaagcaatgg gtgctgtccg tggtgtaaaa
     1141 agattcggta ctgggttcgc accattggat gaggcgctat cacgtgccgt agtcgattta
     1201 tctagtagac catttgctgt aatcgacctt ggattgaaga gagagatgat tggtgattta
     1261 tccactgaaa tgattccaca ctttttggaa agtttcgcgg aggcggccag aattactttg
     1321 catgttgatt gtctgagagg tttcaacgat caccacagaa gtgagagtgc gttcaaggct
     1381 ttggctgttg ccataagaga agctatttct agcaatggca ccaatgacgt tccctcaacc
     1441 aaaggtgttt tgatgtgaag tactgacaat aaaaagattc ttgttttcaa gaacttgtca
     1501 tttgtatagt ttttttatat tgtagttgtt ctattttaat caaatgttag cgtgatttat
     1561 attttttttc gcctcgacat catctgccca gatgcgaagt taagtgcgca gaaagtaata
     1621 tcatgcgtca atcgtatgtg aatgctggtc gctatactgc tgtcgattcg atactaacgc
     1681 cgccatccag agctcgcaga acaccactac tgatactcat tgccaaggct aggaggcacc
     1741 atccgtttca ttttctttga ggtaagccaa tcatgaaata gtatacacat ccataacgga
     1801 cgtacggacg aaataagtgc cgttgtccca ctattccacc gcatttggcc catttggctc
     1861 actttgactc aacttgcgtc attttaactg atatgaaggg tccgactttg tcctttttcg
     1921 gccaccgcat accccacggc gatgcctccg ctacctgcat ttgagtaaca tctccgtttc
     1981 gcggggtatt cggcgctacg tcgcctgttc gagcggctct gttcgttgca tgaaactaaa
     2041 ataagcggaa agtgtccagc catccactac gtcagaaaga aataatggtt gtacactgtt
     2101 tctcggctat ataccgtttt tggttggtta atcctcgcca ggtgcagcta ttgcgcttgg
     2161 ctgcttcgcg atagtagtaa tctgagaaag tgcagatccc ggtaagggaa acacttttgg
     2221 ttcacctttg atagggcttt cattggggca ttcgtaacaa aaaggaagta gatagagaaa
     2281 ttgagaaagc ttaagtgaga tgttttagct tcaattttgt ccccttcaac gctgcttggc
     2341 cttagagggt cagaattgca gttcaggagt agtcacactc atagtatata aacaagccct
     2401 ttattgattt tgaataatta ttttgtatac gtgttctagc atacaagtta gaataaataa
     2461 aaaatagaaa aatagaacat agaaagtttt agacctctag aactagtgga tcctacggta
     2521 cttattgcca agaaagcacg agcatcagcc gtgcctccag gtcgaatctt caaacgacga
     2581 cgatcacgcg tcgctccagt attccagggt tcatcagatc ctacggtact tattgccaag
     2641 aaagcacgag catcagccgt gcctccaggt cgaatcttca aacgacgacg atcacgcgtc
     2701 gctccagtat tccagggttc atcagatcct acggtactta ttgccaagaa agcacgagca
     2761 tcagccgtgc ctccaggtcg aatcttcaaa cgacgacgat cacgcgtcgc tccagtattc
     2821 cagggttcat cagatcctac ggtacttatt gccaagaaag cacgagcatc agccgtgcct
     2881 ccaggtcgaa tcttcaaacg acgacgatca cgcgtcgctc cagtattcca gggttcatca
     2941 gatcctacgg tacttattgc caagaaagca cgagcatcag ccgtgcctcc aggtcgaatc
     3001 ttcaaacgac gacgatcacg cgtcgctcca gtattccagg gttcatcaga tcctacggta
     3061 cttattgcca agaaagcacg agcatcagcc gtgcctccag gtcgaatctt caaacgacga
     3121 cgatcacgcg tcgctccagt attccagggt tcatcagatc ctacggtact tattgccaag
     3181 aaagcacgag catcagccgt gcctccaggt cgaatcttca aacgacgacg atcacgcgtc
     3241 gctccagtat tccagggttc atcagatcct acggtactta ttgccaagaa agcacgagca
     3301 tcagccgtgc ctccaggtcg aatcttcaaa cgacgacgat cacgcgtcgc tccagtattc
     3361 cagggttcat cagatcctac ggtacttatt gccaagaaag cacgagcatc agccgtgcct
     3421 ccaggtcgaa tcttcaaacg acgacgatca cgcgtcgctc cagtattcca gggttcatca
     3481 gatcctacgg tacttattgc caagaaagca cgagcatcag ccgtgcctcc aggtcgaatc
     3541 ttcaaacgac gacgatcacg cgtcgctcca gtattccagg gttcatcaga tcctacggta
     3601 cttattgcca agaaagcacg agcatcagcc gtgcctccag gtcgaatctt caaacgacga
     3661 cgatcacgcg tcgctccagt attccagggt tcatcagatc ctacggtact tattgccaag
     3721 aaagcacgag catcagccgt gcctccaggt cgaatcttca aacgacgacg atcacgcgtc
     3781 gctccagtat tccagggttc atcagatcta tgccagtgtt gaaatcagac aatttcgatc
     3841 cattggaaga agcttacgaa ggtgggacaa ttcaaaacta taacgatgaa caccatcttc
     3901 ataaatcttg ggcaaatgtg attccggaca aacgaggact ttacgaccct gattatgaac
     3961 atgacgcttg tggtgtcggt ttcgtagcaa ataagcatgg tgaacagtct cacaagattg
     4021 ttactgacgc tagatatctt ttagtgaata tgacacatcg tggtgccgtc tcatctgatg
     4081 ggaacggtga cggtgccggt attctgctag gtatcccaag ggcgaattcg cggccgctaa
     4141 attcaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg
     4201 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     4261 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     4321 atacgtacgg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
     4381 ggatgtacag agtgatatta ttgacacgcc ggggcgacgg atggtgatcc ccctggccag
     4441 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga
     4501 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga
     4561 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
     4621 ctggggaata taaatgtcag gcatgagatt atcaaaaagg atcttcacct agatcctttt
     4681 cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc
     4741 tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac
     4801 atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg
     4861 ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc
     4921 aaggatctga tggcgcaggg gatcaagctc tgatcaagag acaggatgag gatcgtttcg
     4981 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     5041 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     5101 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     5161 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     5221 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     5281 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     5341 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     5401 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     5461 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     5521 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     5581 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     5641 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     5701 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     5761 tgacgagttc ttctgaatta ttaacgctta caatttcctg atgcggtatt ttctccttac
     5821 gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat gtgcgcggaa
     5881 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agattatcaa
     5941 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     6001 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     6061 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     6121 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac cacgctcacc
     6181 ggctccagat ttatcagcaa taaaccagcc agccggaagg ccgagcgcag aagtggtcct
     6241 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     6301 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     6361 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     6421 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     6481 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     6541 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     6601 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     6661 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     6721 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     6781 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     6841 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     6901 tattattgaa gcatttatca gggttattgt ctcatgacca aaatccctta acgtgagttt
     6961 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     7021 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     7081 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     7141 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     7201 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     7261 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7321 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7381 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7441 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7501 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7561 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7621 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7681 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     7741 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     7801 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     7861 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     7921 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     7981 acaggaaaca gctatgacca tgattacgcc aagctcagaa ttaaccctca ctaaagggac
     8041 tagtcctgca ggtttaaacg aattcgccct tatata
//
