LOCUS       Exported                6727 bp ds-DNA     circular SYN 24-JAN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jan 24, 2020 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
COMMENT     Ligation of : pVW273_pSIVu_pCYC1mut_PP7deltaFG_GFPenvy_tCYC1.xdna 
            [5249  nt] : (XbaI[2123] / SacI[1831]) to 
            pVW8_pSP125_pADH1_PP7_dCherry.txt.xdna [1470  nt] : (SacI[6385] / 
            XbaI[7859])
FEATURES             Location/Qualifiers
     source          1..6727
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    4..253
                     /label=pURA3
     primer_bind     254..271
                     /label=M13-fwd
     misc_feature    272..277
                     /label=SphI
     misc_feature    284..289
                     /label=AatII
     primer_bind     complement(296..316)
                     /label=M13-rev
     misc_recomb     complement(317..350)
                     /label=LoxP
     misc_feature    351..1769
                     /label=pTEF caURA3 tTEF
     misc_recomb     complement(1770..1803)
                     /label=LoxP
     primer_bind     1804..1823
                     /label=T7
     misc_feature    1837..3304
                     /label=pADH1
                     /note="note=""direct strand 
                     translation""translation=""AGIEEMMVNEIGNQGA*"""
     misc_feature    3310..3707
                     /label=PP7 ?FG
     misc_feature    3713..4429
                     /label=ENVY ORF
     misc_feature    4444..4697
                     /label=Cyc1 Terminator
                     /note="note=""Fragment extracted from pED45.xdna [254  nt] 
                     : (XhoI[2020] / KpnI[2278])"
     primer_bind     complement(4713..4732)
                     /label=T3
     misc_feature    4739..4988
                     /label=tURA3
     rep_origin      complement(5021..5609)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5780..6640)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6641..6704)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taatgatgat atttcataaa taatgtaatt ctatatatgt taattacctt ttttgcgagg
       61 catatttatg gtgaaggata agttttgacc atcaaagaag gttaatgtgg ctgtggtttc
      121 agggtccata atgcttttca attcatcttt tttttttttg ttcttttttt tgattccggt
      181 ttctttgaaa tttttttgat tcggtaatct ccgagcagaa ggaagaacga aggaaggagc
      241 acagacttag atttgtaaaa cgacggccag tgcatgcgta tcggacgtct cccttcatgg
      301 tcatagctgt ttcctgataa cttcgtataa tgtatgctat acgaagttat tagcttgcct
      361 cgtccccgcc gggtcacccg gcgagcgaca tggaggccca gaataccctc cttgacagtc
      421 ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc gtacatttag cccatacatc
      481 cccatgtata atcatttgca tccatacatt ttgatggccg cacggcgcga agcaaaaatt
      541 acggctcctc gctccagacc tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt
      601 gtccccacgc cgcgcccctg tagagaaata taaaaggtta ggatttgcca ctgaggttct
      661 tctttcatat acttcctttt aaaatcttgc taggatacag ttctcacatc acatccgaac
      721 ataaacaacc atgacagtca acactaatac ctatagtgag agagcagaaa ctcatgcctc
      781 accagtagca cagcgattat ttcgattaat ggaactgaag aaaaccaatt tatgtgcatc
      841 aattgacgtt gataccacta aggagttcct tgaattaatt gataaattag gtccttatgt
      901 atgcttaatc aagactcata ttgatataat caatgatttt tcctatgaat ccactattga
      961 accattatta gaactttcac gtaaacatca atttatgatt tttgaagata gaaaatttgc
     1021 tgatattggt aataccgtaa agaaacaata tattggtgga gtttataaaa ttagtagttg
     1081 ggcagatatt accaatgctc atggtgtcac tgggaatgga gtggttgaag gattaaaaca
     1141 gggagctaaa gaaaccacca ccaaccaaga gccaagaggg ttattgatgt tagctgaatt
     1201 atcatcagtg ggatcattag catacggaga atattctcaa aaaactgttg aaattgctaa
     1261 atccgataag gaatttgtta ttggatttat tgcccaacgt gatatgggtg gccaagaaga
     1321 aggatttgat tggcttatta tgacacctgg agttggatta gatgataaag gtgatggatt
     1381 aggacaacaa tatagaactg ttgatgaagt tgttagcact ggaactgata ttatcattgt
     1441 tggtagagga ttgtttggta aaggaagaga tccagatatt gaaggtaaaa ggtatagaaa
     1501 tgctggttgg aatgcttatt tgaaaaagac tggccaatta taatcagtac tgacaataaa
     1561 aagattcttg ttttcaagaa cttgtcattt gtatagtttt tttatattgt agttgttcta
     1621 ttttaatcaa atgttagcgt gatttatatt ttttttcgcc tcgacatcat ctgcccagat
     1681 gcgaagttaa gtgcgcagaa agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct
     1741 atactgctgt cgattcgata ctaacgccga taacttcgta taatgtatgc tatacgaagt
     1801 tattaatacg actcactata gggcgaattg gagctcgccg ggatcgaaga aatgatggta
     1861 aatgaaatag gaaatcaagg agcatgaagg caaaagacaa atataagggt cgaacgaaaa
     1921 ataaagtgaa aagtgttgat atgatgtatt tggctttgcg gcgccgaaaa aacgagttta
     1981 cgcaattgca caatcatgct gactctgtgg cggacccgcg ctcttgccgg cccggcgata
     2041 acgctgggcg tgaggctgtg cccggcggag ttttttgcgc ctgcattttc caaggtttac
     2101 cctgcgctaa ggggcgagat tggagaagca ataagaatgc cggttggggt tgcgatgatg
     2161 acgaccacga caactggtgt cattatttaa gttgccgaaa gaacctgagt gcatttgcaa
     2221 catgagtata ctagaagaat gagccaagac ttgcgagacg cgagtttgcc ggtggtgcga
     2281 acaatagagc gaccatgacc ttgaaggtga gacgcgcata accgctagag tactttgaag
     2341 aggaaacagc aatagggttg ctaccagtat aaatagacag gtacatacaa cactggaaat
     2401 ggttgtctgt ttgagtacgc tttcaattca tttgggtgtg cactttatta tgttacaata
     2461 tggaagggaa ctttacactt ctcctatgca catatattaa ttaaagtcca atgctagtag
     2521 agaagggggg taacacccct ccgcgctctt ttccgatttt tttctaaacc gtggaatatt
     2581 tcggatatcc ttttgttgtt tccgggtgta caatatggac ttcctctttt ctggcaacca
     2641 aacccataca tcgggattcc tataatacct tcgttggtct ccctaacatg taggtggcgg
     2701 aggggagata tacaatagaa cagataccag acaagacata atgggctaaa caagactaca
     2761 ccaattacac tgcctcattg atggtggtac ataacgaact aatactgtag ccctagactt
     2821 gatagccatc atcatatcga agtttcacta ccctttttcc atttgccatc tattgaagta
     2881 ataataggcg catgcaactt cttttctttt tttttctttt ctctctcccc cgttgttgtc
     2941 tcaccatatc cgcaatgaca aaaaaatgat ggaagacact aaaggaaaaa attaacgaca
     3001 aagacagcac caacagatgt cgttgttcca gagctgatga ggggtatctc gaagcacacg
     3061 aaactttttc cttccttcat tcacgcacac tactctctaa tgagcaacgg tatacggcct
     3121 tccttccagt tacttgaatt tgaaataaaa aaaagtttgc tgtcttgcta tcaagtataa
     3181 atagacctgc aattattaat cttttgtttc ctcgtcattg ttctcgttcc ctttcttcct
     3241 tgtttctttt tctgcacaat atttcaagct ataccaagca tacaatcaac tccaagctgg
     3301 ccgctctaga atgtccaaaa ccatcgttct ttcggtcggc gaggctactc gcactctgac
     3361 tgagatccag tccaccgcag accgtcagat atttgaagag aaggtcgggc ctctggtggg
     3421 tcggctgcgc ctcacggctt cgctccgtca aaacggagcc aagaccgcgt atcgcgtcaa
     3481 cctaaaactg gatcaggcgg acgtggttga tactagtctt ccgaaagtgc gctacactca
     3541 ggtatggtcg cacgacgtga caatcgttgc gaatagcacc gaggcctcgc gcaaatcgtt
     3601 gtacgatttg accaagtccc tcgtcgcgac ctcgcaggtc gaggatcttg tcgtcaacct
     3661 tgtgccgctg ggccgtggtt cccctgggct gcaggagttc gacatcaagc ttatgtctaa
     3721 aggcgaggaa ttgtttacag gtgtagttcc tattttggtc gaactagatg gagatgtcaa
     3781 cggtcataag ttttctgtac gtggtgaagg cgaaggtgat gctactaatg gcaaactgac
     3841 tctgaagttc atctgtacga ccggaaaatt gccagttcca tggcctacat tagtaacaac
     3901 attatgttac ggagttcaat gctttgctag atacccagat cacatgaagc agcatgactt
     3961 tttcaaaagt gctatgccag aaggttatgt gcaggagaga actatctcct tcaaagatga
     4021 tggtacttat aagacaagag cagaggtaaa gtttgaaggc gatacattgg ttaatagaat
     4081 tgagcttaaa ggcattgatt ttaaggaaga tgggaatatc ctcggacata aacttgaata
     4141 caatttcaat tcacataatg tttatatcac agcagataaa caaaagaatg gtataaaagc
     4201 gaactttaag acaagacata acgtggaaga tggtagcgtc caattagccg accattacca
     4261 acaaaacact cctatcgggg acgggccagt tctccttcca gacaaccact acctatcaac
     4321 ccaatctgtt ctatctaaag accctaatga aaaacgagat cacatggtct tgttagagtt
     4381 cgtgactgcc gctggtataa cattaggaat ggacgaattg tacaaataag gatcctaact
     4441 cgagtcatgt aattagttat gtcacgctta cattcacgcc ctccccccac atccgctcta
     4501 accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt
     4561 atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct gtacagacgc
     4621 gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag
     4681 gctttaattt gcggccggta cccagctttt gttcccttta gtgagggtta atttcctgaa
     4741 aactgtatta taagtaaatg catgtatact aaactcacaa attagagctt caatttaatt
     4801 atatcagtta ttacgcggga atctcggtcg taatgatttc tataatgacg aaaaaaaaaa
     4861 aattggaaag aaaatgcttc atggccttta taaaaaggaa ctatccaata cctcgccaga
     4921 accaagtaac agtattttac ggggcacaaa tcaagaacaa taagacagga ctgtaaagat
     4981 ggacgcattt aattaattcg aaggccgcgt tgctggcgtt tttccatagg ctcggccccc
     5041 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     5101 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5161 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     5221 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5281 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5341 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     5401 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5461 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5521 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     5581 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     5641 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     5701 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     5761 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     5821 gtctatttcg ttcatccata gttgcctgac tgcccgtcgt gtagataact acgatacggg
     5881 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     5941 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6001 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6061 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     6121 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6181 ccatgttgtg aaaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6241 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6301 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6361 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6421 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6481 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6541 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6601 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6661 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa gtatcgttcg
     6721 aattaat
//
