LOCUS       Exported                6724 bp ds-DNA     circular SYN 24-JAN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jan 24, 2020 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
COMMENT     Ligation of : pVW284_pSIVu_pADH1_PP7dFG_GFPenvy_tCYC1.xdna [5733  
            nt] : (KpnI[4697] / HindIII[3707]) to 
            pVW212_pSIVh_pCYC1_PP7_mCherry_tCYC1.xdna [983  nt] : (HindIII[4241]
            / KpnI[5228])
FEATURES             Location/Qualifiers
     source          1..6724
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    4..253
                     /label=pURA3
     primer_bind     254..271
                     /label=M13-fwd
     misc_feature    272..277
                     /label=SphI
     misc_feature    284..289
                     /label=AatII
     primer_bind     complement(296..316)
                     /label=M13-rev
     misc_recomb     complement(317..350)
                     /label=LoxP
     misc_feature    351..1769
                     /label=pTEF caURA3 tTEF
     misc_recomb     complement(1770..1803)
                     /label=LoxP
     primer_bind     1804..1823
                     /label=T7
     misc_feature    1837..3304
                     /label=pADH1
                     /note="note=""direct strand 
                     translation""translation=""AGIEEMMVNEIGNQGA*"""
     misc_feature    3310..3707
                     /label=PP7 ?FG
     CDS             3725..4426
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="SKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQ
                     TAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFED
                     GGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGE
                     IKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEG
                     RHSTGGMDELYK"
     misc_feature    4441..4694
                     /label=Cyc1 Terminator
                     /note="note=""Fragment extracted from pRS416_TEF [254  nt] 
                     : (XhoI[3258] / KpnI[3516])"
     primer_bind     complement(4710..4729)
                     /label=T3
     misc_feature    4736..4985
                     /label=tURA3
     rep_origin      complement(5018..5606)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5777..6637)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6638..6701)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taatgatgat atttcataaa taatgtaatt ctatatatgt taattacctt ttttgcgagg
       61 catatttatg gtgaaggata agttttgacc atcaaagaag gttaatgtgg ctgtggtttc
      121 agggtccata atgcttttca attcatcttt tttttttttg ttcttttttt tgattccggt
      181 ttctttgaaa tttttttgat tcggtaatct ccgagcagaa ggaagaacga aggaaggagc
      241 acagacttag atttgtaaaa cgacggccag tgcatgcgta tcggacgtct cccttcatgg
      301 tcatagctgt ttcctgataa cttcgtataa tgtatgctat acgaagttat tagcttgcct
      361 cgtccccgcc gggtcacccg gcgagcgaca tggaggccca gaataccctc cttgacagtc
      421 ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc gtacatttag cccatacatc
      481 cccatgtata atcatttgca tccatacatt ttgatggccg cacggcgcga agcaaaaatt
      541 acggctcctc gctccagacc tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt
      601 gtccccacgc cgcgcccctg tagagaaata taaaaggtta ggatttgcca ctgaggttct
      661 tctttcatat acttcctttt aaaatcttgc taggatacag ttctcacatc acatccgaac
      721 ataaacaacc atgacagtca acactaatac ctatagtgag agagcagaaa ctcatgcctc
      781 accagtagca cagcgattat ttcgattaat ggaactgaag aaaaccaatt tatgtgcatc
      841 aattgacgtt gataccacta aggagttcct tgaattaatt gataaattag gtccttatgt
      901 atgcttaatc aagactcata ttgatataat caatgatttt tcctatgaat ccactattga
      961 accattatta gaactttcac gtaaacatca atttatgatt tttgaagata gaaaatttgc
     1021 tgatattggt aataccgtaa agaaacaata tattggtgga gtttataaaa ttagtagttg
     1081 ggcagatatt accaatgctc atggtgtcac tgggaatgga gtggttgaag gattaaaaca
     1141 gggagctaaa gaaaccacca ccaaccaaga gccaagaggg ttattgatgt tagctgaatt
     1201 atcatcagtg ggatcattag catacggaga atattctcaa aaaactgttg aaattgctaa
     1261 atccgataag gaatttgtta ttggatttat tgcccaacgt gatatgggtg gccaagaaga
     1321 aggatttgat tggcttatta tgacacctgg agttggatta gatgataaag gtgatggatt
     1381 aggacaacaa tatagaactg ttgatgaagt tgttagcact ggaactgata ttatcattgt
     1441 tggtagagga ttgtttggta aaggaagaga tccagatatt gaaggtaaaa ggtatagaaa
     1501 tgctggttgg aatgcttatt tgaaaaagac tggccaatta taatcagtac tgacaataaa
     1561 aagattcttg ttttcaagaa cttgtcattt gtatagtttt tttatattgt agttgttcta
     1621 ttttaatcaa atgttagcgt gatttatatt ttttttcgcc tcgacatcat ctgcccagat
     1681 gcgaagttaa gtgcgcagaa agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct
     1741 atactgctgt cgattcgata ctaacgccga taacttcgta taatgtatgc tatacgaagt
     1801 tattaatacg actcactata gggcgaattg gagctcgccg ggatcgaaga aatgatggta
     1861 aatgaaatag gaaatcaagg agcatgaagg caaaagacaa atataagggt cgaacgaaaa
     1921 ataaagtgaa aagtgttgat atgatgtatt tggctttgcg gcgccgaaaa aacgagttta
     1981 cgcaattgca caatcatgct gactctgtgg cggacccgcg ctcttgccgg cccggcgata
     2041 acgctgggcg tgaggctgtg cccggcggag ttttttgcgc ctgcattttc caaggtttac
     2101 cctgcgctaa ggggcgagat tggagaagca ataagaatgc cggttggggt tgcgatgatg
     2161 acgaccacga caactggtgt cattatttaa gttgccgaaa gaacctgagt gcatttgcaa
     2221 catgagtata ctagaagaat gagccaagac ttgcgagacg cgagtttgcc ggtggtgcga
     2281 acaatagagc gaccatgacc ttgaaggtga gacgcgcata accgctagag tactttgaag
     2341 aggaaacagc aatagggttg ctaccagtat aaatagacag gtacatacaa cactggaaat
     2401 ggttgtctgt ttgagtacgc tttcaattca tttgggtgtg cactttatta tgttacaata
     2461 tggaagggaa ctttacactt ctcctatgca catatattaa ttaaagtcca atgctagtag
     2521 agaagggggg taacacccct ccgcgctctt ttccgatttt tttctaaacc gtggaatatt
     2581 tcggatatcc ttttgttgtt tccgggtgta caatatggac ttcctctttt ctggcaacca
     2641 aacccataca tcgggattcc tataatacct tcgttggtct ccctaacatg taggtggcgg
     2701 aggggagata tacaatagaa cagataccag acaagacata atgggctaaa caagactaca
     2761 ccaattacac tgcctcattg atggtggtac ataacgaact aatactgtag ccctagactt
     2821 gatagccatc atcatatcga agtttcacta ccctttttcc atttgccatc tattgaagta
     2881 ataataggcg catgcaactt cttttctttt tttttctttt ctctctcccc cgttgttgtc
     2941 tcaccatatc cgcaatgaca aaaaaatgat ggaagacact aaaggaaaaa attaacgaca
     3001 aagacagcac caacagatgt cgttgttcca gagctgatga ggggtatctc gaagcacacg
     3061 aaactttttc cttccttcat tcacgcacac tactctctaa tgagcaacgg tatacggcct
     3121 tccttccagt tacttgaatt tgaaataaaa aaaagtttgc tgtcttgcta tcaagtataa
     3181 atagacctgc aattattaat cttttgtttc ctcgtcattg ttctcgttcc ctttcttcct
     3241 tgtttctttt tctgcacaat atttcaagct ataccaagca tacaatcaac tccaagctgg
     3301 ccgctctaga atgtccaaaa ccatcgttct ttcggtcggc gaggctactc gcactctgac
     3361 tgagatccag tccaccgcag accgtcagat atttgaagag aaggtcgggc ctctggtggg
     3421 tcggctgcgc ctcacggctt cgctccgtca aaacggagcc aagaccgcgt atcgcgtcaa
     3481 cctaaaactg gatcaggcgg acgtggttga tactagtctt ccgaaagtgc gctacactca
     3541 ggtatggtcg cacgacgtga caatcgttgc gaatagcacc gaggcctcgc gcaaatcgtt
     3601 gtacgatttg accaagtccc tcgtcgcgac ctcgcaggtc gaggatcttg tcgtcaacct
     3661 tgtgccgctg ggccgtggtt cccctgggct gcaggagttc gacatcaagc ttatgagatc
     3721 tgctagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     3781 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     3841 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     3901 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     3961 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     4021 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     4081 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     4141 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     4201 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     4261 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     4321 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     4381 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagggat cctaactcga
     4441 gtcatgtaat tagttatgtc acgcttacat tcacgccctc cccccacatc cgctctaacc
     4501 gaaaaggaag gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg
     4561 ttagtattaa gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg
     4621 tacgcatgta acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct
     4681 ttaatttgcg gccggtaccc agcttttgtt ccctttagtg agggttaatt tcctgaaaac
     4741 tgtattataa gtaaatgcat gtatactaaa ctcacaaatt agagcttcaa tttaattata
     4801 tcagttatta cgcgggaatc tcggtcgtaa tgatttctat aatgacgaaa aaaaaaaaat
     4861 tggaaagaaa atgcttcatg gcctttataa aaaggaacta tccaatacct cgccagaacc
     4921 aagtaacagt attttacggg gcacaaatca agaacaataa gacaggactg taaagatgga
     4981 cgcatttaat taattcgaag gccgcgttgc tggcgttttt ccataggctc ggcccccctg
     5041 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5101 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5161 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5221 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5281 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5341 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     5401 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     5461 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5521 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5581 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5641 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5701 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     5761 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     5821 tatttcgttc atccatagtt gcctgactgc ccgtcgtgta gataactacg atacgggagg
     5881 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     5941 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6001 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     6061 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     6121 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     6181 tgttgtgaaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     6241 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     6301 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     6361 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     6421 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     6481 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     6541 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     6601 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     6661 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaagta tcgttcgaat
     6721 taat
//
