LOCUS       Exported                6682 bp ds-DNA     circular SYN 24-JAN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6682)
  AUTHORS   serge
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jan 24, 2020 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6682
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    4..253
                     /label=pURA3
     primer_bind     115..134
                     /label=424
                     /note="pSIV_pURA3"
     primer_bind     254..271
                     /label=M13-fwd
     primer_bind     complement(254..271)
                     /label=725
                     /note="pURA-m13fw"
     primer_bind     254..271
                     /label=404
                     /note="M13fw"
     misc_feature    272..277
                     /label=SphI
     misc_feature    284..289
                     /label=AatII
     primer_bind     complement(296..316)
                     /label=M13-rev
     primer_bind     complement(296..316)
                     /label=405
                     /note="M13rv"
     primer_bind     296..314
                     /label=791
                     /note="SIV-L"
     misc_recomb     complement(317..350)
                     /label=LoxP
     misc_feature    351..1769
                     /label=pTEF caURA3 tTEF
     primer_bind     351..370
                     /label=1279
                     /note="pTEF"
     primer_bind     complement(387..406)
                     /label=390
                     /note="TEF"
     primer_bind     717..737
                     /label=430
                     /note="Ura3"
     primer_bind     complement(1081..1109)
                     /label=398
                     /note="Gene Block MCS1 fragment"
     primer_bind     1093..1118
                     /label=399
                     /note="Gene Block MCS2 fragment"
     primer_bind     1733..1752
                     /label=763
                     /note="STE2"
     primer_bind     1733..1752
                     /label=62
                     /note="TEF"
     primer_bind     complement(1746..1769)
                     /label=1280
                     /note="pTEF"
     misc_recomb     complement(1770..1803)
                     /label=LoxP
     primer_bind     1804..1824
                     /label=212
                     /note="2xMCS Insert"
     primer_bind     1804..1823
                     /label=T7
     primer_bind     1804..1823
                     /label=11
                     /note="T7"
     primer_bind     1804..1823
                     /label=155
                     /note="AatII-T7"
     primer_bind     complement(1812..1830)
                     /label=8
                     /note="pRSxx"
     misc_feature    1837..3304
                     /label=pADH1
                     /note="note=""direct strand 
                     translation""translation=""AGIEEMMVNEIGNQGA*"""
     primer_bind     1837..1855
                     /label=149
                     /note="ADH1"
     primer_bind     2274..2291
                     /label=120
                     /note="pADH1"
     primer_bind     3043..3061
                     /label=pADH_seq
     primer_bind     complement(3288..3304)
                     /label=150
                     /note="ADH1"
     primer_bind     3305..3335
                     /label=1392
                     /note="MS2"
     misc_feature    3310..3662
                     /label=MS2
                     /note="note=""Fragment extracted from 1392/1393 PCR.xdna 
                     [353  nt] : (XbaI[7] / HindIII[364])"
     primer_bind     complement(3642..3667)
                     /label=1393
                     /note="MS2"
     primer_bind     complement(3642..3667)
                     /label=1355
                     /note="MS2"
     primer_bind     complement(3642..3661)
                     /label=1394
                     /note="MS2"
     primer_bind     3662..3693
                     /label=1109
                     /note="GFP ENVY"
     misc_feature    3668..4384
                     /label=ENVY ORF
     primer_bind     complement(4360..4390)
                     /label=1110
                     /note="GFP ENVY"
     primer_bind     complement(4361..4384)
                     /label=1345
                     /note="GFPenvy"
     primer_bind     complement(4394..4416)
                     /label=1086
                     /note="4xSZ1"
     primer_bind     4394..4416
                     /label=1008
                     /note="GLT1"
     misc_feature    4399..4652
                     /label=Cyc1 Terminator
                     /note="note=""Fragment extracted from pRS416_TEF [254  nt] 
                     : (XhoI[3258] / KpnI[3516])"
     primer_bind     complement(4399..4427)
                     /label=409
                     /note="CYC1 terminator"
     primer_bind     complement(4475..4492)
                     /label=1067
                     /note="tCYC1"
     primer_bind     complement(4475..4492)
                     /label=1066
                     /note="Far1"
     primer_bind     complement(4631..4653)
                     /label=462
                     /note="Cyc1 terminator"
     primer_bind     complement(4631..4653)
                     /label=900
                     /note="Super PKI NES 2"
     primer_bind     complement(4632..4652)
                     /label=1424
                     /note="tCyc1"
     primer_bind     complement(4635..4653)
                     /label=1186
                     /note="dPSTR"
     primer_bind     complement(4650..4686)
                     /label=72
                     /note="SynZip1"
     primer_bind     4652..4670
                     /label=9
                     /note="pRSxx"
     primer_bind     complement(4668..4687)
                     /label=T3
     primer_bind     complement(4668..4687)
                     /label=244
                     /note="SphI T3"
     primer_bind     complement(4668..4687)
                     /label=156
                     /note="AatII-T3"
     primer_bind     4669..4688
                     /label=726
                     /note="tURA-T7"
     primer_bind     complement(4669..4688)
                     /label=10
                     /note="T3"
     misc_feature    4694..4943
                     /label=tURA3
     primer_bind     complement(4899..4922)
                     /label=425
                     /note="pSIV_tURA3"
     primer_bind     4949..4974
                     /label=288
                     /note="pRS306"
     primer_bind     complement(4963..4982)
                     /label=828
                     /note="pGT"
     rep_origin      complement(4976..5564)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5003..5025)
                     /label=829
                     /note="pGT"
     CDS             complement(5738..6397)
                     /label=AmpR
     primer_bind     complement(5770..5794)
                     /label=488
                     /note="Amp"
     primer_bind     complement(5819..5837)
                     /label=209
                     /note="2xMCS Backbone"
     primer_bind     5822..5855
                     /label=210
                     /note="2xMCS Amp"
     primer_bind     6581..6600
                     /label=157
                     /note="second MCS"
     promoter        complement(6596..6659)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6637..6656
                     /label=625
                     /note="AmpProm"
     primer_bind     complement(6643..6682)
                     /label=289
                     /note="pRS306"
     primer_bind     complement(6647..6681)
                     /label=400
                     /note="bacterial part pRS"
ORIGIN
        1 taatgatgat atttcataaa taatgtaatt ctatatatgt taattacctt ttttgcgagg
       61 catatttatg gtgaaggata agttttgacc atcaaagaag gttaatgtgg ctgtggtttc
      121 agggtccata atgcttttca attcatcttt tttttttttg ttcttttttt tgattccggt
      181 ttctttgaaa tttttttgat tcggtaatct ccgagcagaa ggaagaacga aggaaggagc
      241 acagacttag atttgtaaaa cgacggccag tgcatgcgta tcggacgtct cccttcatgg
      301 tcatagctgt ttcctgataa cttcgtataa tgtatgctat acgaagttat tagcttgcct
      361 cgtccccgcc gggtcacccg gcgagcgaca tggaggccca gaataccctc cttgacagtc
      421 ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc gtacatttag cccatacatc
      481 cccatgtata atcatttgca tccatacatt ttgatggccg cacggcgcga agcaaaaatt
      541 acggctcctc gctccagacc tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt
      601 gtccccacgc cgcgcccctg tagagaaata taaaaggtta ggatttgcca ctgaggttct
      661 tctttcatat acttcctttt aaaatcttgc taggatacag ttctcacatc acatccgaac
      721 ataaacaacc atgacagtca acactaatac ctatagtgag agagcagaaa ctcatgcctc
      781 accagtagca cagcgattat ttcgattaat ggaactgaag aaaaccaatt tatgtgcatc
      841 aattgacgtt gataccacta aggagttcct tgaattaatt gataaattag gtccttatgt
      901 atgcttaatc aagactcata ttgatataat caatgatttt tcctatgaat ccactattga
      961 accattatta gaactttcac gtaaacatca atttatgatt tttgaagata gaaaatttgc
     1021 tgatattggt aataccgtaa agaaacaata tattggtgga gtttataaaa ttagtagttg
     1081 ggcagatatt accaatgctc atggtgtcac tgggaatgga gtggttgaag gattaaaaca
     1141 gggagctaaa gaaaccacca ccaaccaaga gccaagaggg ttattgatgt tagctgaatt
     1201 atcatcagtg ggatcattag catacggaga atattctcaa aaaactgttg aaattgctaa
     1261 atccgataag gaatttgtta ttggatttat tgcccaacgt gatatgggtg gccaagaaga
     1321 aggatttgat tggcttatta tgacacctgg agttggatta gatgataaag gtgatggatt
     1381 aggacaacaa tatagaactg ttgatgaagt tgttagcact ggaactgata ttatcattgt
     1441 tggtagagga ttgtttggta aaggaagaga tccagatatt gaaggtaaaa ggtatagaaa
     1501 tgctggttgg aatgcttatt tgaaaaagac tggccaatta taatcagtac tgacaataaa
     1561 aagattcttg ttttcaagaa cttgtcattt gtatagtttt tttatattgt agttgttcta
     1621 ttttaatcaa atgttagcgt gatttatatt ttttttcgcc tcgacatcat ctgcccagat
     1681 gcgaagttaa gtgcgcagaa agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct
     1741 atactgctgt cgattcgata ctaacgccga taacttcgta taatgtatgc tatacgaagt
     1801 tattaatacg actcactata gggcgaattg gagctcgccg ggatcgaaga aatgatggta
     1861 aatgaaatag gaaatcaagg agcatgaagg caaaagacaa atataagggt cgaacgaaaa
     1921 ataaagtgaa aagtgttgat atgatgtatt tggctttgcg gcgccgaaaa aacgagttta
     1981 cgcaattgca caatcatgct gactctgtgg cggacccgcg ctcttgccgg cccggcgata
     2041 acgctgggcg tgaggctgtg cccggcggag ttttttgcgc ctgcattttc caaggtttac
     2101 cctgcgctaa ggggcgagat tggagaagca ataagaatgc cggttggggt tgcgatgatg
     2161 acgaccacga caactggtgt cattatttaa gttgccgaaa gaacctgagt gcatttgcaa
     2221 catgagtata ctagaagaat gagccaagac ttgcgagacg cgagtttgcc ggtggtgcga
     2281 acaatagagc gaccatgacc ttgaaggtga gacgcgcata accgctagag tactttgaag
     2341 aggaaacagc aatagggttg ctaccagtat aaatagacag gtacatacaa cactggaaat
     2401 ggttgtctgt ttgagtacgc tttcaattca tttgggtgtg cactttatta tgttacaata
     2461 tggaagggaa ctttacactt ctcctatgca catatattaa ttaaagtcca atgctagtag
     2521 agaagggggg taacacccct ccgcgctctt ttccgatttt tttctaaacc gtggaatatt
     2581 tcggatatcc ttttgttgtt tccgggtgta caatatggac ttcctctttt ctggcaacca
     2641 aacccataca tcgggattcc tataatacct tcgttggtct ccctaacatg taggtggcgg
     2701 aggggagata tacaatagaa cagataccag acaagacata atgggctaaa caagactaca
     2761 ccaattacac tgcctcattg atggtggtac ataacgaact aatactgtag ccctagactt
     2821 gatagccatc atcatatcga agtttcacta ccctttttcc atttgccatc tattgaagta
     2881 ataataggcg catgcaactt cttttctttt tttttctttt ctctctcccc cgttgttgtc
     2941 tcaccatatc cgcaatgaca aaaaaatgat ggaagacact aaaggaaaaa attaacgaca
     3001 aagacagcac caacagatgt cgttgttcca gagctgatga ggggtatctc gaagcacacg
     3061 aaactttttc cttccttcat tcacgcacac tactctctaa tgagcaacgg tatacggcct
     3121 tccttccagt tacttgaatt tgaaataaaa aaaagtttgc tgtcttgcta tcaagtataa
     3181 atagacctgc aattattaat cttttgtttc ctcgtcattg ttctcgttcc ctttcttcct
     3241 tgtttctttt tctgcacaat atttcaagct ataccaagca tacaatcaac tccaagctgg
     3301 ccgctctaga atggcttcta actttactca gttcgttctc gtcgacaatg gcggaactgg
     3361 cgacgtgact gtcgccccaa gcaacttcgc taacgggatc gctgaatgga tcagctctaa
     3421 ctcgcgttca caggcttaca aagtaacctg tagcgttcgt cagagctctg cgcagaatcg
     3481 caaatacacc atcaaagtcg aggtgcctaa aggcgcctgg cgttcgtact taaatatgga
     3541 actaaccatt ccaattttcg ccacgaattc cgactgcgag cttattgtta aggcaatgca
     3601 aggtctccta aaagatggaa acccgattcc ctcagcaatc gcagcaaact ccggcatcta
     3661 caagcttatg tctaaaggcg aggaattgtt tacaggtgta gttcctattt tggtcgaact
     3721 agatggagat gtcaacggtc ataagttttc tgtacgtggt gaaggcgaag gtgatgctac
     3781 taatggcaaa ctgactctga agttcatctg tacgaccgga aaattgccag ttccatggcc
     3841 tacattagta acaacattat gttacggagt tcaatgcttt gctagatacc cagatcacat
     3901 gaagcagcat gactttttca aaagtgctat gccagaaggt tatgtgcagg agagaactat
     3961 ctccttcaaa gatgatggta cttataagac aagagcagag gtaaagtttg aaggcgatac
     4021 attggttaat agaattgagc ttaaaggcat tgattttaag gaagatggga atatcctcgg
     4081 acataaactt gaatacaatt tcaattcaca taatgtttat atcacagcag ataaacaaaa
     4141 gaatggtata aaagcgaact ttaagacaag acataacgtg gaagatggta gcgtccaatt
     4201 agccgaccat taccaacaaa acactcctat cggggacggg ccagttctcc ttccagacaa
     4261 ccactaccta tcaacccaat ctgttctatc taaagaccct aatgaaaaac gagatcacat
     4321 ggtcttgtta gagttcgtga ctgccgctgg tataacatta ggaatggacg aattgtacaa
     4381 ataaggatcc taactcgagt catgtaatta gttatgtcac gcttacattc acgccctccc
     4441 cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt
     4501 tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt
     4561 tttctgtaca gacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt
     4621 ttgggacgct cgaaggcttt aatttgcggc cggtacccag cttttgttcc ctttagtgag
     4681 ggttaatttc ctgaaaactg tattataagt aaatgcatgt atactaaact cacaaattag
     4741 agcttcaatt taattatatc agttattacg cgggaatctc ggtcgtaatg atttctataa
     4801 tgacgaaaaa aaaaaaattg gaaagaaaat gcttcatggc ctttataaaa aggaactatc
     4861 caatacctcg ccagaaccaa gtaacagtat tttacggggc acaaatcaag aacaataaga
     4921 caggactgta aagatggacg catttaatta attcgaaggc cgcgttgctg gcgtttttcc
     4981 ataggctcgg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     5041 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     5101 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     5161 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     5221 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     5281 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     5341 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     5401 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     5461 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     5521 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     5581 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     5641 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     5701 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     5761 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactgccc gtcgtgtaga
     5821 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     5881 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     5941 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     6001 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     6061 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     6121 gagttacatg atcccccatg ttgtgaaaaa aagcggttag ctccttcggt cctccgatcg
     6181 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     6241 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     6301 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     6361 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     6421 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     6481 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     6541 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     6601 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     6661 ttgaagtatc gttcgaatta at
//
