LOCUS       Exported                4895 bp ds-DNA     circular SYN 05-JUN-2012
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 27, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(273..457)
                     /direction=LEFT
                     /label=Polyoma Ori'
     misc_feature    718..751
                     /label=loxP
                     /label=loxP(1)
     primer_bind     764..784
                     /label=M13-rev
     misc_feature    800..924
                     /label=attR4
     misc_feature    complement(1271..1576)
                     /label=ccdB
     misc_feature    complement(1921..2579)
                     /label=CmR
     misc_feature    2827..2951
                     /label=attR3
     primer_bind     complement(2959..2975)
                     /label=M13-fwd
     misc_feature    complement(2988..3021)
                     /label=loxP
     rep_origin      complement(3241..3923)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(4021..4680)
                     /label=AmpR
ORIGIN
        1 gaccgctttg gaaaaacaaa gactgtattt cctggaaatt aatgtttatt caataaactg
       61 tgtattcagc tatattcaca tagtggtgag gctgaaatga ggcgggaaga ggcggttggg
      121 gcttaattat atcaatttgg gtggccccac agcgcctcca aggcgccagt cctgttttga
      181 caagttgcct ctggaagcct ctcttctttt tctccagagt aagcggaggc caggggcccc
      241 cggcctctgc ttaatactaa aaaaaacagc tgttgtcata gtaatgattg ggtggaaaca
      301 ttccaggcct gggtggagag gctttttgct tcctcttgca aaaccacact gacattccag
      361 gcctgggtgg agaggctttt tgcttcctct tgcaaaacca cactgccctc tggagggcag
      421 ttgcctagca actaattaaa agaggatgtc gcacggccag ctgcggtcag ttagtcactt
      481 cctgcttaac tgacttgaca ttttctattt taagagtcgg gaggaaaatt actgtgttgg
      541 aggccctccg ccatcttctg aagctgaatc gaattaactt gtttattgca gcttataatg
      601 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
      661 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatatcata
      721 acttcgtata gcatacatta tacgaagtta tgtcgacact agtcaggaaa cagctatgac
      781 catgattacg ccaagctatc aactttgtat agaaaagttg aacgagaaac gtaaaatgat
      841 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa
      901 acacaacata tccagtcact atggtcgacc gacagccttc caaatgttct tctcaaacgg
      961 aatcgtcgta tccagcctac tcgctattgt cctcaatgcc gtattaaatc ataaaaagaa
     1021 ataagaaaaa gaggtgcgag cctctttttt gtgtgacaaa ataaaaacat ctacctattc
     1081 atatacgcta gtgtcatagt cctgaaaatc atctgcatca agaacaattt cacaactctt
     1141 atacttttct cttacaagtc gttcggcttc atctggattt tcagcctcta tacttactaa
     1201 acgtgataaa gtttctgtaa tttctactgt atcgacctgc agactggctg tgtataaggg
     1261 agcctgacat ttatattccc cagaacatca ggttaatggc gtttttgatg tcattttcgc
     1321 ggtggctgag atcagccact tcttccccga taacggagac cggcacactg gccatatcgg
     1381 tggtcatcat gcgccagctt tcatccccga tatgcaccac cgggtaaagt tcacgggaga
     1441 ctttatctga cagcagacgt gcactggcca gggggatcac catccgtcgc ccgggcgtgt
     1501 caataatatc actctgtaca tccacaaaca gacgataacg gctctctctt ttataggtgt
     1561 aaaccttaaa ctgcatttca ccagcccctg ttctcgtcag caaaagagcc gttcatttca
     1621 ataaaccggg cgacctcagc catcccttcc tgattttccg ctttccagcg ttcggcacgc
     1681 agacgacggg cttcattctg catggttgtg cttaccagac cggagatatt gacatcatat
     1741 atgccttgag caactgatag ctgtcgctgt caactgtcac tgtaatacgc tgcttcatag
     1801 catacctctt tttgacatac ttcgggtata catatcagta tatattctta taccgcaaaa
     1861 atcagcgcgc aaatacgcat actgttatct ggcttttagt aagccggatc cacgcggcgt
     1921 ttacgccccc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
     1981 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     2041 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     2101 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc
     2161 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     2221 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
     2281 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     2341 ccgtctttca ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga
     2401 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     2461 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     2521 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     2581 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     2641 atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccaa
     2701 aagttggccc agggcttccc ggtatcaaca gggacaccag gatttattta ttctgcgaag
     2761 tgatcttccg tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc tgccaactta
     2821 cggccgccat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg
     2881 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt caactttatt
     2941 atacatagtt gataattcac tggccgtcgt tttacctcga gggatccata acttcgtata
     3001 gcatacatta tacgaagtta tgctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3061 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3121 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3181 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3241 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3301 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3361 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3421 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3481 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3541 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3601 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3661 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     3721 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3781 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3841 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3901 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     3961 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     4021 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     4081 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     4141 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     4201 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     4261 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     4321 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     4381 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     4441 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     4501 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     4561 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     4621 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     4681 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     4741 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     4801 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     4861 gaaatgttga atactcatac tcttcctttt tcaat
//
