LOCUS       Exported                5824 bp ds-DNA     circular SYN 04-SEP-2012
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 27, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..21
                     /label=M13-rev
     misc_feature    37..161
                     /label=attR4
     misc_feature    complement(508..813)
                     /label=ccdB
     misc_feature    complement(1158..1816)
                     /label=CmR
     misc_feature    2064..2188
                     /label=attR3
     primer_bind     complement(2196..2213)
                     /label=M13-fwd
     CDS             2284..2350
                     /label=LacZ alpha
     promoter        join(2376..2452,2454..2637)
                     /label=MC1
     misc_feature    2653..3309
                     /label=DTA ORF
     polyA_signal    3447..3652
                     /label=PGKpA
     misc_feature    4060..4920
                     /label=AmpR
     misc_feature    5065..5738
                     /label=pUC ori
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga aaagttgaac
       61 gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga
      121 ctacataata ctgtaaaaca caacatatcc agtcactatg gtcgaccgac agccttccaa
      181 atgttcttct caaacggaat cgtcgtatcc agcctactcg ctattgtcct caatgccgta
      241 ttaaatcata aaaagaaata agaaaaagag gtgcgagcct cttttttgtg tgacaaaata
      301 aaaacatcta cctattcata tacgctagtg tcatagtcct gaaaatcatc tgcatcaaga
      361 acaatttcac aactcttata cttttctctt acaagtcgtt cggcttcatc tggattttca
      421 gcctctatac ttactaaacg tgataaagtt tctgtaattt ctactgtatc gacctgcaga
      481 ctggctgtgt ataagggagc ctgacattta tattccccag aacatcaggt taatggcgtt
      541 tttgatgtca ttttcgcggt ggctgagatc agccacttct tccccgataa cggagaccgg
      601 cacactggcc atatcggtgg tcatcatgcg ccagctttca tccccgatat gcaccaccgg
      661 gtaaagttca cgggagactt tatctgacag cagacgtgca ctggccaggg ggatcaccat
      721 ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc acaaacagac gataacggct
      781 ctctctttta taggtgtaaa ccttaaactg catttcacca gcccctgttc tcgtcagcaa
      841 aagagccgtt catttcaata aaccgggcga cctcagccat cccttcctga ttttccgctt
      901 tccagcgttc ggcacgcaga cgacgggctt cattctgcat ggttgtgctt accagaccgg
      961 agatattgac atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt
     1021 aatacgctgc ttcatagcat acctcttttt gacatacttc gggtatacat atcagtatat
     1081 attcttatac cgcaaaaatc agcgcgcaaa tacgcatact gttatctggc ttttagtaag
     1141 ccggatccac gcggcgttta cgccccccct gccactcatc gcagtactgt tgtaattcat
     1201 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg
     1261 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
     1321 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
     1381 agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
     1441 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
     1501 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
     1561 cccatatcac cagctcaccg tctttcattg ccatacggaa ttccggatga gcattcatca
     1621 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
     1681 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
     1741 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
     1801 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
     1861 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa
     1921 cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg acaccaggat
     1981 ttatttattc tgcgaagtga tcttccgtca caggtattta ttcggcgcaa agtgcgtcgg
     2041 gtgatgctgc caacttacgg ccgccatagt gactggatat gttgtgtttt acagtattat
     2101 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
     2161 tctcgttcaa ctttattata catagttgat aattcactgg ccgtcgtttt acaacgtcgt
     2221 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     2281 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     2341 aatggcgaat ggcgccgctt atcgataccg tcgagcagtg tggttttcaa gaggaagcaa
     2401 aaagcctctc cacccaggcc tggaatgttt ccacccaatg tcgagcagtg tgatttgcaa
     2461 gaggaagcaa aaagcctctc cacccaggcc tggaatgttt ccacccaatg tcgagcaaac
     2521 cccgcccagc gtcttgtcat tggcgaattc gaacacgcag atgcagtcgg ggcggcgcgg
     2581 tcccaggtcc acttcgcata ttaaggtgac gcgtgtggcc tcgaacaccg agcgaccctg
     2641 caggtcctcg ccatggatcc tgatgatgtt gttgattctt ctaaatcttt tgtgatggaa
     2701 aacttttctt cgtaccacgg gactaaacct ggttatgtag attccattca aaaaggtata
     2761 caaaagccaa aatctggtac acaaggaaat tatgacgatg attggaaagg gttttatagt
     2821 accgacaata aatacgacgc tgcgggatac tctgtagata atgaaaaccc gctctctgga
     2881 aaagctggag gcgtggtcaa agtgacgtat ccaggactga cgaaggttct cgcactaaaa
     2941 gtggataatg ccgaaactat taagaaagag ttaggtttaa gtctcactga accgttgatg
     3001 gagcaagtcg gaacggaaga gtttatcaaa aggttcggtg atggtgcttc gcgtgtagtg
     3061 ctcagccttc ccttcgctga ggggagttct agcgttgaat atattaataa ctgggaacag
     3121 gcgaaagcgt taagcgtaga acttgagatt aattttgaaa cccgtggaaa acgtggccaa
     3181 gatgcgatgt atgagtatat ggctcaagcc tgtgcaggaa atcgtgtcag gcgatctctt
     3241 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
     3301 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
     3361 tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
     3421 agatccacta gagtcgaggg atgcagaaat tgatgatcta ttaaacaata aagatgtcca
     3481 ctaaaatgga agtttttcct gtcatacttt gttaagaagg gtgagaacag agtacctaca
     3541 ttttgaatgg aaggattgga gctacggggg tgggggtggg gtgggattag ataaatgcct
     3601 gctctttact gaaggctctt tactattgct ttatgataat gtttcatagt tggatatcat
     3661 aatttaaaca acatatggtg cactctcagt gcaatctgct ctgatgccgc atagttaagc
     3721 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     3781 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     3841 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     3901 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     3961 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4021 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4081 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4141 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4201 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4261 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     4321 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4381 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4441 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4501 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4561 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     4621 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4681 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4741 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4801 gggtcagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4861 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4921 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     4981 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5041 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5101 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5161 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5221 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     5281 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5341 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5401 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5461 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5521 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5581 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5641 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5701 ttacggttcc tggtcttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     5761 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccagcgg ataacaattt
     5821 caca
//
