LOCUS       Exported                4369 bp ds-DNA     circular SYN 20-APR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 27, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
COMMENT     Insert from //LS-VLA64/share/genbank format/544.gb 9 to 1755
            Backbone from //LS-VLA64/share/genbank format/pDONR P4-P1R.gb2830to 
            674
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     536..553
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     misc_feature    593..688
                     /locus_tag="attL4"
                     /label=attL4
     enhancer        702..1060
                     /locus_tag="HCMVIEE"
                     /label=HCMVIEE
     promoter        1072..2313
                     /locus_tag="CAG pro"
                     /label=CAG pro
     misc_feature    1072..2313
                     /locus_tag="chicken beta actin -50~1292 position"
                     /label=chicken beta actin -50~1292 position
     misc_feature    2321..2361
                     /locus_tag="rabbit bglobin intron3'"
                     /label=rabbit bglobin intron3'
     misc_feature    2362..2414
                     /locus_tag="rabbit bglobin exon"
                     /label=rabbit bglobin exon
     misc_feature    2415..2571
                     /locus_tag="attR1"
                     /label=attR1
     primer_bind     complement(2630..2650)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     CDS             2763..3569
                     /locus_tag="KanR"
                     /label=KanR
     misc_feature    3693..4366
                     /locus_tag="pUC ori"
                     /label=pUC ori
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgtt taaacgtcga ctagttatta atagtaatca
      721 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      781 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      841 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      901 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      961 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     1021 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc
     1081 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt
     1141 attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggggcg cgcgccaggc
     1201 ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat
     1261 cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat
     1321 aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc
     1381 tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg
     1441 agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt
     1501 gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg
     1561 gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc
     1621 gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt
     1681 gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg
     1741 aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg
     1801 gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg
     1861 ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg
     1921 caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg
     1981 cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt
     2041 ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg
     2101 aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc
     2161 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc
     2221 cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg
     2281 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat
     2341 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
     2401 tttggcaaag aattcaagtt tgtacaaaaa agttgaacga gaaacgtaaa atgatataaa
     2461 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
     2521 acatatgcag tcactatgaa tcaactactt agatggtatt agtgacctgt agaattcgag
     2581 ctctagagct gcagggcggc cgcgatatcc cctatagtga gtcgtattac atggtcatag
     2641 ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata
     2701 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
     2761 ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg
     2821 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc
     2881 gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg
     2941 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc
     3001 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc
     3061 ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg
     3121 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta
     3181 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg
     3241 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa
     3301 tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg
     3361 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa
     3421 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt
     3481 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc
     3541 agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact
     3601 ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg
     3661 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3721 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3781 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3841 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     3901 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3961 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4021 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4081 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     4141 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4201 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4261 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4321 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtt
//
