LOCUS       Exported                3244 bp ds-DNA     circular SYN 17-APR-2013
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 27, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
COMMENT     Insert from S:/genbank format/90710 pL451.gb 808 to 1429
            Backbone from S:/genbank format/pDONR P4-P1R.gb 2830 to 674
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     536..553
                     /label=M13-fwd
     misc_feature    complement(593..674)
                     /label=attP4
     gene            join(702..769,771..973,975..1216)
                     /label=PGK promoter
                     /note="PGK promoter"
     gene            1219..1284
                     /label=EM7 promoter
                     /note="EM7 promoter"
     misc_feature    complement(1297..1446)
                     /label=attP1R
     primer_bind     complement(1505..1525)
                     /label=M13-rev
     CDS             1638..2444
                     /label=KanR
     misc_feature    2568..3241
                     /label=pUC ori
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgtt taaacgtcga ctctaccggg taggggaggc
      721 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
      781 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
      841 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
      901 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
      961 cgtgcagatg gacggcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     1021 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     1081 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     1141 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     1201 cctttcgacc tgcagcctgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     1261 tacgacaagg tgaggaacta aaccgaattc aagtttgtac aaaaaagttg aacgagaaac
     1321 gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata
     1381 atactgtaaa acacaacata tgcagtcact atgaatcaac tacttagatg gtattagtga
     1441 cctgtagaat tcgagctcta gagctgcagg gcggccgcga tatcccctat agtgagtcgt
     1501 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     1561 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
     1621 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt
     1681 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     1741 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     1801 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     1861 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     1921 tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag
     1981 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     2041 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga
     2101 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     2161 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg
     2221 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     2281 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     2341 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     2401 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta
     2461 attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac
     2521 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     2581 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2641 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2701 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     2761 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2821 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2881 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2941 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
     3001 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3061 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3121 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3181 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3241 tgtt
//
