LOCUS       Exported                3127 bp ds-DNA     circular SYN 16-APR-2013
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 27, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
COMMENT     Insert from //LS-VLA64/share/genbank format/544.gb 9 to 1755
            Backbone from //LS-VLA64/share/genbank 
            format/pDONRP4-P1R.gb2830to674
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     536..553
                     /label=M13-fwd
     misc_feature    593..688
                     /label=attL4
     promoter        714..1146
                     /label=hCMV*-1
     misc_feature    1173..1329
                     /label=attR1
     primer_bind     complement(1388..1408)
                     /label=M13-rev
     CDS             1521..2327
                     /label=KanR
     misc_feature    2451..3124
                     /label=pUC ori
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgtt taaacgtcga agcttacatc gagtttacca
      721 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
      781 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
      841 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
      901 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
      961 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagctc ggtacccggg
     1021 tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca
     1081 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
     1141 cagcctccgc gggactcaga cacccgggat cccaagtttg tacaaaaaag ttgaacgaga
     1201 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
     1261 ataatactgt aaaacacaac atatgcagtc actatgaatc aactacttag atggtattag
     1321 tgacctgtag aattcgagct ctagagctgc agggcggccg cgatatcccc tatagtgagt
     1381 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat
     1441 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
     1501 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa
     1561 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
     1621 caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac
     1681 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
     1741 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
     1801 tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt
     1861 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
     1921 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
     1981 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
     2041 aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc
     2101 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     2161 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     2221 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     2281 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
     2341 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat
     2401 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     2461 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2521 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2581 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     2641 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2701 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2761 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2821 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg
     2881 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2941 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3001 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3061 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3121 acatgtt
//
