LOCUS Exported 3974 bp ds-DNA circular SYN 14-JUN-2012 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3974) AUTHORS . TITLE Direct Submission JOURNAL Exported Monday, Jan 27, 2020 from SnapGene Viewer 5.0.7 https://www.snapgene.com COMMENT Insert from //LS-VLA64/share/genbank format/niwa lab plasmid/10681.gb 3231 to 4586 Backbone from //LS-VLA64/share/genbank format/pDONR P2R-P3.gb 2889 to 733 FEATURES Location/Qualifiers source 1..3974 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 536..553 /label=M13-fwd misc_feature 537..552 /label=M13 Forward misc_feature 591..733 /label=attP2R misc_feature 776..1348 /label=IRES CDS 1352..1744 /label=BSD polyA_signal 1782..1993 /label=SV40 pA misc_feature 2090..2178 /label=attP3 primer_bind complement(2235..2255) /label=M13-rev CDS 2368..3174 /label=KanR misc_feature 3298..3901 /label=pUC ori ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac 601 taataccatc taagtagttg attcatagtg actgcatatg ttgtgtttta cagtattatg 661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt 721 ctcgttcaac tttcttgtac aaagtgggtt taaaccgccc cccccccctc tccctccccc 781 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg 841 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc 901 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg 961 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg 1021 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca 1081 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata 1141 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc 1201 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt 1261 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt 1321 tgaaaaacac gatgataata tggccacaac catgcctttg tctcaagaag aatccaccct 1381 cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc 1441 cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac 1501 tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa 1561 cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg 1621 gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga 1681 tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg 1741 ttaactagat aagtaatgat cataatcagc catatcacat ctgtagaggt tttacttgct 1801 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt 1861 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 1921 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 1981 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 2041 tgcgtattgg gcgctcttcc gcttcctcgc tcacggcgcg cccaacttta ttatacaaag 2101 ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc 2161 aaaataaaat cattatttgg agctccatgg tagcgttaac gcggccgcga tatcccctat 2221 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat 2281 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct 2341 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg 2401 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc 2461 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt 2521 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac 2581 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat 2641 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat 2701 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg 2761 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa 2821 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg 2881 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc 2941 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt 3001 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg 3061 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt 3121 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca 3181 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca 3241 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc 3301 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 3361 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 3421 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg 3481 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 3541 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 3601 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 3661 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga 3721 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 3781 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 3841 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 3901 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 3961 tttgctcaca tgtt //