LOCUS       Exported                3974 bp ds-DNA     circular SYN 14-JUN-2012
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 27, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
COMMENT     Insert from //LS-VLA64/share/genbank format/niwa lab 
            plasmid/10681.gb 3231 to 4586
            Backbone from //LS-VLA64/share/genbank format/pDONR P2R-P3.gb 2889 
            to 733
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     536..553
                     /label=M13-fwd
     misc_feature    537..552
                     /label=M13 Forward
     misc_feature    591..733
                     /label=attP2R
     misc_feature    776..1348
                     /label=IRES
     CDS             1352..1744
                     /label=BSD
     polyA_signal    1782..1993
                     /label=SV40 pA
     misc_feature    2090..2178
                     /label=attP3
     primer_bind     complement(2235..2255)
                     /label=M13-rev
     CDS             2368..3174
                     /label=KanR
     misc_feature    3298..3901
                     /label=pUC ori
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac
      601 taataccatc taagtagttg attcatagtg actgcatatg ttgtgtttta cagtattatg
      661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      721 ctcgttcaac tttcttgtac aaagtgggtt taaaccgccc cccccccctc tccctccccc
      781 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
      841 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
      901 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
      961 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     1021 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     1081 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     1141 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     1201 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     1261 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     1321 tgaaaaacac gatgataata tggccacaac catgcctttg tctcaagaag aatccaccct
     1381 cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc
     1441 cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac
     1501 tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa
     1561 cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg
     1621 gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga
     1681 tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg
     1741 ttaactagat aagtaatgat cataatcagc catatcacat ctgtagaggt tttacttgct
     1801 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     1861 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     1921 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     1981 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     2041 tgcgtattgg gcgctcttcc gcttcctcgc tcacggcgcg cccaacttta ttatacaaag
     2101 ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
     2161 aaaataaaat cattatttgg agctccatgg tagcgttaac gcggccgcga tatcccctat
     2221 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat
     2281 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct
     2341 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg
     2401 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
     2461 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt
     2521 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     2581 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     2641 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat
     2701 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     2761 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     2821 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     2881 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     2941 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     3001 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     3061 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     3121 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     3181 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca
     3241 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     3301 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3361 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3421 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     3481 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3541 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3601 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3661 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga
     3721 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3781 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3841 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3901 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3961 tttgctcaca tgtt
//
