LOCUS       Exported                5175 bp ds-DNA     circular SYN 28-JUN-2012
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 27, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
COMMENT     Insert from New DNA 13 to 2563
            Backbone from //LS-VLA64/share/genbank format/pDONR P2R-P3.gb2889to 
            733
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     536..553
                     /label=M13-fwd
     misc_feature    537..552
                     /label=M13 Forward
     misc_feature    591..733
                     /label=attP2R
     misc_feature    774..1349
                     /label=IRES
     misc_feature    1350..1946
                     /label=Pac
     misc_feature    1953..2948
                     /label=DeltaTK
     misc_feature    2949..3283
                     /label=polyA
     misc_feature    3291..3379
                     /label=attP3
     primer_bind     complement(3436..3456)
                     /label=M13-rev
     CDS             3569..4375
                     /label=KanR
     misc_feature    4499..5102
                     /label=pUC ori
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac
      601 taataccatc taagtagttg attcatagtg actgcatatg ttgtgtttta cagtattatg
      661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      721 ctcgttcaac tttcttgtac aaagtgggaa ttccgccccc cccccctctc cctccccccc
      781 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
      841 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
      901 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
      961 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     1021 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     1081 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     1141 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     1201 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     1261 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     1321 aaaaacacga tgataagctt gccacaacca tgaccgagta caagcccacg gtgcgcctcg
     1381 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     1441 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     1501 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     1561 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     1621 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     1681 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     1741 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     1801 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     1861 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     1921 ggtgcatgac ccgcaagccc ggtgccggat ccatgcccac gctactgcgg gtttatatag
     1981 acggtcccca cgggatgggg aaaaccacca ccacgcaact gctggtggcc ctgggttcgc
     2041 gcgacgatat cgtctacgta cccgagccga tgacttactg gcgggtgctg ggggcttccg
     2101 agacaatcgc gaacatctac accacacaac accgcctcga ccagggtgag atatcggccg
     2161 gggacgcggc ggtggtaatg acaagcgccc agataacaat gggcatgcct tatgccgtga
     2221 ccgacgccgt tctggctcct catatcgggg gggaggctgg gagctcacat gccccgcccc
     2281 cggccctcac cctcatcttc gaccgccatc ccatcgccgc cctcctgtgc tacccggccg
     2341 cgcggtacct tatgggcagc atgacccccc aggccgtgct ggcgttcgtg gccctcatcc
     2401 cgccgacctt gcccggcacc aacatcgtgc ttggggccct tccggaggac agacacatcg
     2461 accgcctggc caaacgccag cgccccggcg agcggctgga cctggctatg ctggctgcga
     2521 ttcgccgcgt ttacgggcta cttgccaata cggtgcggta tctgcagtgc ggcgggtcgt
     2581 ggcgggagga ctggggacag ctttcgggga cggccgtgcc gccccagggt gccgagcccc
     2641 agagcaacgc gggcccacga ccccatatcg gggacacgtt atttaccctg tttcgggccc
     2701 ccgagttgct ggcccccaac ggcgacctgt ataacgtgtt tgcctgggcc ttggacgtct
     2761 tggccaaacg cctccgttcc atgcacgtct ttatcctgga ttacgaccaa tcgcccgccg
     2821 gctgccggga cgccctgctg caacttacct ccgggatggt ccagacccac gtcaccaccc
     2881 ccggctccat accgacgata tgcgacctgg cgcgcacgtt tgcccgggag atgggggagg
     2941 ctaactgagc tctagcttat cgactcgccg atagtggaaa ccgacgcccc agcactcgtc
     3001 cgagggcaaa ggaatagtcg agaaattgat gatctattaa acaataaaga tgtccacatg
     3061 gaagtttttc ctgtcatact ttgttaagaa gggtgagaac agagtaccta cattttgaat
     3121 ggaaggattg gagctacggg ggtgggggtg gggtgggatt agataaatgc ctgctcttta
     3181 ctgaaggctc tttactattg ctttatgata atgtttcata gttggatatc ataatttaaa
     3241 caagcaaaac caaattaagg gccagctcat tcctcccact catcaacttt attatacaaa
     3301 gttggcatta taaaaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     3361 caaaataaaa tcattatttg gagctccatg gtagcgttaa cgcggccgcg atatccccta
     3421 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
     3481 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc
     3541 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc
     3601 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     3661 cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt
     3721 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     3781 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     3841 tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata
     3901 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     3961 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
     4021 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     4081 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt
     4141 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     4201 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     4261 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     4321 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     4381 agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc
     4441 aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
     4501 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4561 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4621 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     4681 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4741 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4801 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4861 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg
     4921 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4981 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5041 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5101 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     5161 ttttgctcac atgtt
//
