LOCUS       Exported                3582 bp ds-DNA     circular SYN 26-DEC-2012
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 27, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
COMMENT     Insert from New DNA(1) 13 to 1077
            Backbone from //LS-VLA64/share/genbank format/pDONR 221.gb 2896 to 
            651
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     536..553
                     /label=M13-fwd
     misc_feature    570..651
                     /label=attP1
     misc_feature    674..1702
                     /label=Cre(stop deleted)
     misc_feature    complement(1717..1805)
                     /label=attP2
     primer_bind     complement(1843..1863)
                     /label=M13-rev
     CDS             1976..2782
                     /label=KanR
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgtcca atttactgac cgtacaccaa aatttgcctg cattaccggt
      721 cgatgcaacg agtgatgagg ttcgcaagaa cctgatggac atgttcaggg atcgccaggc
      781 gttttctgag catacctgga aaatgcttct gtccgtttgc cggtcgtggg cggcatggtg
      841 caagttgaat aaccggaaat ggtttcccgc agaacctgaa gatgttcgcg attatcttct
      901 atatcttcag gcgcgcggtc tggcagtaaa aactatccag caacatttgg gccagctaaa
      961 catgcttcat cgtcggtccg ggctgccacg accaagtgac agcaatgctg tttcactggt
     1021 tatgcggcgg atccgaaaag aaaacgttga tgccggtgaa cgtgcaaaac aggctctagc
     1081 gttcgaacgc actgatttcg accaggttcg ttcactcatg gaaaatagcg atcgctgcca
     1141 ggatatacgt aatctggcat ttctggggat tgcttataac accctgttac gtatagccga
     1201 aattgccagg atcagggtta aagatatctc acgtactgat ggtgggagaa tgttaatcca
     1261 tattggcaga acgaaaacgc tggttagcac cgcaggtgta gagaaggcac ttagcctggg
     1321 ggtaactaaa ctggtcgagc gatggatttc cgtctctggt gtagctgatg atccgaataa
     1381 ctacctgttt tgccgggtca gaaaaaatgg tgttgccgcg ccatctgcca ccagccagct
     1441 atcaactcgc gccctggaag ggatttttga agcaactcat cgattgattt acggcgctaa
     1501 ggatgactct ggtcagagat acctggcctg gtctggacac agtgcccgtg tcggagccgc
     1561 gcgagatatg gcccgcgctg gagtttcaat accggagatc atgcaagctg gtggctggac
     1621 caatgtaaat attgtcatga actatatccg taacctggat agtgaaacag gggcaatggt
     1681 gcgcctgctg gaagatggcg attagcaccc agctttcttg tacaaagttg gcattataag
     1741 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     1801 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
     1861 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     1921 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     1981 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata
     2041 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta
     2101 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     2161 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
     2221 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa
     2281 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     2341 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
     2401 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
     2461 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
     2521 acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     2581 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     2641 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     2701 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     2761 tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag
     2821 cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta
     2881 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2941 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3001 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3061 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3121 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3181 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3241 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3301 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3361 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3421 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3481 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3541 tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
