LOCUS Exported 4549 bp ds-DNA circular SYN 01-APR-2013 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4549) AUTHORS . TITLE Direct Submission JOURNAL Exported Monday, Jan 27, 2020 from SnapGene Viewer 5.0.7 https://www.snapgene.com COMMENT Insert from S:/genbank format/90926 pBSII-CreERT2.ape 2256 to 4287 Backbone from S:/genbank format/pDONR 221.gb 2896 to 651 FEATURES Location/Qualifiers source 1..4549 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 536..553 /label=M13-fwd misc_feature 570..651 /label=attP1 CDS 680..2662 /codon_start=1 /label=ORF frame 3 /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE DGDLEPSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPT RPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVW RSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSII LLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLIL SHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLAT AGSTSSHSLQKYYITGEAEGFPATA" misc_feature 680..2662 /label=CrehERT2 misc_feature 692..1687 /label=CRE_NLS /note="/translation=MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSE HTWKM LLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRS GLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRN LAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVT KLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAK DDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGA MVRLLEDGDLEPSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLE ILMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGE EFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQH QRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGAS VEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATA*" misc_feature 1709..2662 /label=hERT2 CDS complement(1996..2457) /codon_start=1 /label=ORF frame 3 /label=ORF frame 3(1) /translation="MLYRCSMPLLLMCLMWERMRRSWASRWCCCCRVRPALAIRWIKVS VILSRTRWIWSFSSRDFRVLDRNVYTPELSKIIDLRHTNSSPCRFIMRNRDDVASSMSK ISTMPSTHFPWFLSKSKLGANSSFTGCSMERQTRPIIRISSQAHSRRWT" misc_feature complement(2684..2772) /label=attP2 primer_bind complement(2810..2830) /label=M13-rev CDS 2943..3749 /label=KanR ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctct cgagccacca tgtccaattt actgaccgta caccaaaatt tgcctgcatt 721 accggtcgat gcaacgagtg atgaggttcg caagaacctg atggacatgt tcagggatcg 781 ccaggcgttt tctgagcata cctggaaaat gcttctgtcc gtttgccggt cgtgggcggc 841 atggtgcaag ttgaataacc ggaaatggtt tcccgcagaa cctgaagatg ttcgcgatta 901 tcttctatat cttcaggcgc gcggtctggc agtaaaaact atccagcaac atttgggcca 961 gctaaacatg cttcatcgtc ggtccgggct gccacgacca agtgacagca atgctgtttc 1021 actggttatg cggcggatcc gaaaagaaaa cgttgatgcc ggtgaacgtg caaaacaggc 1081 tctagcgttc gaacgcactg atttcgacca ggttcgttca ctcatggaaa atagcgatcg 1141 ctgccaggat atacgtaatc tggcatttct ggggattgct tataacaccc tgttacgtat 1201 agccgaaatt gccaggatca gggttaaaga tatctcacgt actgacggtg ggagaatgtt 1261 aatccatatt ggcagaacga aaacgctggt tagcaccgca ggtgtagaga aggcacttag 1321 cctgggggta actaaactgg tcgagcgatg gatttccgtc tctggtgtag ctgatgatcc 1381 gaataactac ctgttttgcc gggtcagaaa aaatggtgtt gccgcgccat ctgccaccag 1441 ccagctatca actcgcgccc tggaagggat ttttgaagca actcatcgat tgatttacgg 1501 cgctaaggat gactctggtc agagatacct ggcctggtct ggacacagtg cccgtgtcgg 1561 agccgcgcga gatatggccc gcgctggagt ttcaataccg gagatcatgc aagctggtgg 1621 ctggaccaat gtaaatattg tcatgaacta tatccgtaac ctggatagtg aaacaggggc 1681 aatggtgcgc ctgctggaag atggcgatct cgagccatct gctggagaca tgagagctgc 1741 caacctttgg ccaagcccgc tcatgatcaa acgctctaag aagaacagcc tggccttgtc 1801 cctgacggcc gaccagatgg tcagtgcctt gttggatgct gagcccccca tactctattc 1861 cgagtatgat cctaccagac ccttcagtga agcttcgatg atgggcttac tgaccaacct 1921 ggcagacagg gagctggttc acatgatcaa ctgggcgaag agggtgccag gctttgtgga 1981 tttgaccctc catgatcagg tccaccttct agaatgtgcc tggctagaga tcctgatgat 2041 tggtctcgtc tggcgctcca tggagcaccc agtgaagcta ctgtttgctc ctaacttgct 2101 cttggacagg aaccagggaa aatgtgtaga gggcatggtg gagatcttcg acatgctgct 2161 ggctacatca tctcggttcc gcatgatgaa tctgcaggga gaggagtttg tgtgcctcaa 2221 atctattatt ttgcttaatt ctggagtgta cacatttctg tccagcaccc tgaagtctct 2281 ggaagagaag gaccatatcc accgagtcct ggacaagatc acagacactt tgatccacct 2341 gatggccaag gcaggcctga ccctgcagca gcagcaccag cggctggccc agctcctcct 2401 catcctctcc cacatcaggc acatgagtaa caaaggcatg gagcatctgt acagcatgaa 2461 gtgcaagaac gtggtgcccc tctatgacct gctgctggag gcggcggacg cccaccgcct 2521 acatgcgccc actagccgtg gaggggcatc cgtggaggag acggaccaaa gccacttggc 2581 cactgcgggc tctacttcat cgcattcctt gcaaaagtat tacatcacgg gggaggcaga 2641 gggtttccct gccacagctt gataggcggc cgcacccagc tttcttgtac aaagttggca 2701 ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata 2761 aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag 2821 ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata 2881 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg 2941 ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg 3001 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc 3061 gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg 3121 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc 3181 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc 3241 ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg 3301 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta 3361 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg 3421 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa 3481 tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg 3541 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa 3601 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt 3661 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc 3721 agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact 3781 ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg 3841 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 3901 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 3961 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 4021 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca 4081 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 4141 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 4201 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 4261 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 4321 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 4381 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 4441 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 4501 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtt //