LOCUS       Exported                4252 bp ds-DNA     circular SYN 03-APR-2013
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 27, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
COMMENT     Insert from S:/genbank format/niwa lab plasmid/10683.gb 13 to 1683
            Backbone from S:/genbank 
            format/vender'splasmid/pDONR221-P1P5r.str2835 to 651
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    537..552
                     /label=M13-F
     misc_feature    570..651
                     /label=attP1
     misc_feature    complement(671..704)
                     /label=loxP
     CDS             708..1733
                     /label=HygroR
     polyA_signal    2060..2271
                     /label=SV40 pA
     misc_feature    complement(2280..2312)
                     /label=loxP
                     /label=loxP(1)
     misc_feature    2322..2471
                     /label=attP5r
     misc_feature    complement(2517..2533)
                     /label=M13-R
     misc_feature    2646..3455
                     /label=Kanamycin resistant gene
     misc_feature    3576..4249
                     /label=pUC origin
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctta ataacttcgt atagcataca ttatacgaag ttataccatg aaaaagcctg
      721 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc
      781 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
      841 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgttcatc
      901 ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg
      961 agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg
     1021 aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg
     1081 ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca
     1141 ctacgtggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg
     1201 tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg
     1261 ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc
     1321 tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt
     1381 cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc
     1441 agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt
     1501 atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg
     1561 atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg
     1621 ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac
     1681 tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagtcgagaa
     1741 attgatgatc tattaagcaa taaagacgtc cactaaaatg gaagtttttc ctgtcatact
     1801 ttgttaagaa gggtgagaac agagtaccta cattttgaat ggaaggattg gagctacggg
     1861 ggtgggggtg gggtgggatt agataaatgc ctgctcttta ctgaaggctc tttactattg
     1921 ctttatgata atgtttcata gttggatatc ataatttaaa caagcaaaac caaattaagg
     1981 gccagctcat tcctccactc acgatctata gatccactag ttctagataa gtaatgatca
     2041 taatcagcca tatcacatct gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     2101 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     2161 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     2221 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcccaa
     2281 taacttcgta tagcatacat tatacgaagt tatacaactt tgtatacaaa agttgaacga
     2341 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
     2401 acataatact gtaaaacaca acatatccag tcactatgaa tcaactactt agatggtatt
     2461 agtgacctgt actgcagggc ggccgcgata tcccctatag tgagtcgtat tacatggtca
     2521 tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag
     2581 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg
     2641 gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg
     2701 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
     2761 atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg
     2821 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
     2881 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
     2941 tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
     3001 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
     3061 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
     3121 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
     3181 aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
     3241 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
     3301 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
     3361 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
     3421 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac
     3481 actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta
     3541 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3601 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3661 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3721 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3781 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3841 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3901 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3961 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4021 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4081 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4141 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4201 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
