LOCUS       Exported                3303 bp ds-DNA     circular SYN 03-OCT-2013
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 27, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
COMMENT     Insert from 
            /Volumes/share/genbankformat/UnderConstruction/MouseCbx5_Cbx1/pORF( 
            5-2)mCherry-stop.ape 1to 773Backbone from 
            /Volumes/share/genbankformat/UnderConstruction/MouseCbx5_Cbx1/pDONR 
            P5-P2.ape 2896 to 651
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     539..556
                     /label=M13-fwd
     misc_feature    573..654
                     /label=attP5 recombination site
     misc_feature    677..1414
                     /label=mCherry_stop
     misc_feature    1395..1414
                     /label=mCherry_end(stop)
     misc_feature    1428..1516
                     /label=attP2 recombination site
     primer_bind     complement(1547..1568)
                     /label=T7
     primer_bind     complement(1567..1587)
                     /label=M13-rev
     CDS             1700..2506
                     /label=KanR
     misc_feature    2630..3303
                     /label=pUC origin
ORIGIN
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
       61 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      121 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      181 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac
      241 cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc
      301 ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc
      361 ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac
      421 aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc
      481 ctggcagttc cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg
      541 taaaacgacg gccagtctta agctcgggcc ccaaataatg attttatttt gactgatagt
      601 gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa ctttgtatac
      661 aaaagttgta ggcaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
      721 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
      781 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
      841 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
      901 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
      961 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     1021 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     1081 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     1141 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     1201 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     1261 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     1321 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     1381 caagggatat ccatcacact ggcggccgct cgagtaaacc cagctttctt gtacaaagtt
     1441 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     1501 aataaaatca ttatttgcca tccagctgca gggcggccgc gatatcccct atagtgagtc
     1561 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     1621 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
     1681 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa
     1741 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     1801 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     1861 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     1921 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     1981 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc
     2041 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     2101 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     2161 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     2221 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     2281 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     2341 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     2401 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     2461 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt
     2521 taattggttg taacactggc agagcattac gctgacttga cgggacggcg caagctcatg
     2581 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa
     2641 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     2701 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2761 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     2821 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2881 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2941 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3001 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     3061 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3121 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3181 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3241 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3301 cat
//
