LOCUS       Exported                9627 bp ds-DNA     circular SYN 10-DEC-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9627)
  AUTHORS   Arthur de Jong
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 29, 2020 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9627
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     enhancer        192..571
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             789..969
                     /label=5' LTR
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1016..1141
                     /label=HIV-1 psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1634..1867
                     /label=HIV-1 RRE
                     /note="The Rev response element (RRE) of human
                     immunodeficiency virus type 1 allows for Rev-dependent mRNA
                     export from the nucleus to the cytoplasm."
     misc_feature    2394..2511
                     /label=HIV-1 cPPT
                     /note="central polypurine tract from pol gene of human 
                     immunodeficiency virus type 1"
     promoter        2577..3024
                     /label=hSyn promoter
     primer_bind     complement(3017..3106)
                     /label=AJ18020
     protein_bind    3031..3064
                     /label=lox2272
     primer_bind     3093..3182
                     /label=18021
     protein_bind    3115..3148
                     /label=loxP
     CDS             complement(3172..3429)
                     /codon_start=1
                     /label=KASH TMD
                     /translation="LRSREEEEETDSRMPHLDSPGSSQPRRSFLSRVIRAALPLQLLLL
                     LLLLLACLLPASEDDYSCTQANNFARSFYPMLRYTNGPPPT"
     primer_bind     3413..3432
                     /label=AJ18025
     CDS             complement(3436..4146)
                     /codon_start=1
                     /label=mCherry
                     /translation="MAVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMN
                     FEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGAL
                     KGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYER
                     AEGRHSTGGMDELYK"
     primer_bind     complement(3480..3498)
                     /label=oJWseq8 mCherry-C FW
     primer_bind     3517..3526
                     /label=18021
     primer_bind     4123..4135
                     /label=pEGFP-RP
                     /note="Macrogen Seq primer"
     primer_bind     complement(4132..4221)
                     /label=AJ18022
     regulatory      4147..4152
     protein_bind    complement(4159..4192)
                     /label=lox2272
     primer_bind     4204..4293
                     /label=AJ18023
     protein_bind    complement(4243..4276)
                     /label=loxP
     primer_bind     complement(4277..4305)
                     /label=AJ19027
     misc_feature    4312..4900
                     /label=WPRE
                     /note="woodchuck posttranscriptional regulatory element"
     CDS             complement(4783..4794)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4903..4919)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             5425..5605
                     /label=5' LTR
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    5637..5861
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5907..6335
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6349..6678
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6529..6664
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6726..6773
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6792..7166
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and
                     Zeocin"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7296..7417
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7466..7482)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7490..7506
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7514..7544)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7559..7580
                     /label=CAP bindin
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site;CAP binding activates transcription
                     in the presence of cAMP."
     rep_origin      complement(7868..8456)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8627..9487)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9488..9592)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
       61 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
      121 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
      181 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      241 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      301 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      361 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      421 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      481 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      541 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      601 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      661 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      721 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
      781 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      841 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      901 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      961 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     1021 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     1081 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     1141 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     1201 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     1261 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     1321 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     1381 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     1441 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     1501 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1561 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1621 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1681 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1741 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1801 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1861 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1921 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1981 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     2041 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     2101 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     2161 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     2221 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     2281 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2341 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     2401 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2461 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2521 tacagggaca gcagagatcc agtttggtta attaactgca gagggccctg cgtatgagtg
     2581 caagtgggtt ttaggaccag gatgaggcgg ggtgggggtg cctacctgac gaccgacccc
     2641 gacccactgg acaagcaccc aacccccatt ccccaaattg cgcatcccct atcagagagg
     2701 gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc accgcggaca
     2761 gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga aggcgcgctg
     2821 acgtcactcg ccggtccccc gcaaactccc cttcccggcc accttggtcg cgtccgcgcc
     2881 gccgccggcc cagccggacc gcaccacgcg aggcgcgaga taggggggca cgggcgcgac
     2941 catctgcgct gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg cagcggagga
     3001 gtcgtgtcgt gcctgagagc gcagtctaga ataacttcgt ataggatact ttatacgaag
     3061 ttatgcagaa tggtagctgg attgtagctg ctattagcaa tatgaaacct cttaataact
     3121 tcgtatagca tacattatac gaagttatac tagaccggtc ccgaattcct aggtgggagg
     3181 tggcccgttg gtgtaccgca gcatggggta gaaggatcgg gcaaagttgt tggcctgggt
     3241 gcagctgtag tcatcttcag aggcaggtag caggcaggcc aggagcagca gcagcagcag
     3301 aagcagctgc aacggtagcg ctgccctgat cacccttgag aggaaggagc gtctcggctg
     3361 ggagctgccg gggctgtcga ggtggggcat cctgctgtct gtctcctcct cctcctctcg
     3421 agatctgagt ccggacttgt acagctcgtc catgccgccg gtggagtggc ggccctcggc
     3481 gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccaacttgat
     3541 gttgacgttg taggcgccgg gcagctgcac gggcttcttg gccttgtagg tggtcttgac
     3601 ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc tgcttgatct cgcccttcag
     3661 ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc cagcccatgg tcttcttctg
     3721 cattacgggg ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt agatgaactc
     3781 gccgtcctgc agggaggagt cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat
     3841 cacgcgctcc cacttgaagc cctcggggaa ggacagcttc aagtagtcgg ggatgtcggc
     3901 ggggtgcttc acgtaggcct tggagccgta catgaactga ggggacagga tgtcccaggc
     3961 gaagggcagg gggccaccct tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg
     4021 gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg
     4081 caccttgaag cgcatgaact ccttgatgat ggccatgtta tcctcctcgc ccttgctcac
     4141 cgccatggtg gcgctagcat aacttcgtat aaagtatcct atacgaagtt atttgcctta
     4201 acccagaaat tatcactgtt attctttaga atggtgcaaa gaataacttc gtataatgta
     4261 tgctatacga agttatggat ccgttaacaa ttcgatatca agcttatcga taatcaacct
     4321 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     4381 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4441 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4501 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4561 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4621 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4681 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     4741 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4801 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4861 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     4921 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     4981 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     5041 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     5101 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     5161 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg gatcagatat
     5221 ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa
     5281 gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac
     5341 ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc
     5401 cgagagctgc atccggactg tactgggtct ctctggttag accagatctg agcctgggag
     5461 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     5521 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     5581 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt
     5641 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     5701 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     5761 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     5821 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     5881 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     5941 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     6001 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     6061 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     6121 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     6181 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     6241 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     6301 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     6361 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     6421 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     6481 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     6541 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     6601 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     6661 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     6721 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     6781 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     6841 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     6901 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     6961 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     7021 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     7081 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     7141 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
     7201 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     7261 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     7321 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     7381 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     7441 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     7501 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     7561 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7621 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7681 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7741 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7801 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7861 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7921 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7981 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8041 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8101 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8161 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8221 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8281 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     8341 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8401 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8461 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8521 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8581 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8641 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8701 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8761 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8821 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8881 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8941 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9001 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9061 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9121 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9181 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9241 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9301 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9361 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9421 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9481 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9541 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9601 cccgaaaagt gccacctgac acgacac
//
