LOCUS       Exported               10791 bp ds-DNA     circular SYN 10-DEC-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10791)
  AUTHORS   Arthur de Jong
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 29, 2020 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10791
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     enhancer        192..571
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             789..969
                     /label=5' LTR
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1016..1141
                     /label=HIV-1 psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1634..1867
                     /label=HIV-1 RRE
                     /note="The Rev response element (RRE) of human 
                     immunodeficiency virus type 1 allows for Rev-dependent mRNA
                     export from the nucleus to the cytoplasm."
     misc_feature    2394..2511
                     /label=HIV-1 cPPT
                     /note="central polypurine tract from pol gene of human 
                     immunodeficiency virus type 1"
     promoter        2577..3024
                     /label=hSyn promoter
     primer_bind     complement(3017..3106)
                     /label=AJ18020
     protein_bind    3031..3064
                     /label=lox2272
     protein_bind    3115..3148
                     /label=loxP
     CDS             complement(3163..3186)
                     /codon_start=1
                     /product="FLAG epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     3197..3217
                     /label=Primer 2
                     /note="5' gactataaagacgacgatgacaag 3' = flag, omgekeerd = 
                     ctgatatttctgctgctactgttcGCTAGC = NHeI = omgekeerd CGATCG
                     GGT/GGC/GGG/GGA = glycine  = omgekeerd CCA, CCG, CCC, CCT
                     TGA=stop codon = ACT"
     CDS             complement(3199..5316)
                     /codon_start=1
                     /label=Synapsin-1
                     /translation="MNYLRRRLSDSNFMANLPNGYMTDLQRPQPPPPPPSAASPGATPG
                     SATASAERASTAAPVASPAAPSPGSSGGGGFFSSLSNAVKQTTAAAAATFSEQVGGGSG
                     GAGRGGAAARVLLVIDEPHTDWAKYFKGKKIHGEIDIKVEQAEFSDLNLVAHANGGFSV
                     DMEVLRNGVKVVRSLKPDFVLIRQHAFSMARNGDYRSLVIGLQYAGIPSVNSLHSVYNF
                     CDKPWVFAQMVRLHKKLGTEEFPLIDQTFYPNHKEMLSSTTYPVVVKMGHAHSGMGKVK
                     VDNQHDFQDIASVVALTKTYATAEPFIDAKYDVRVQKIGQNYKAYMRTSVSGNWKTNTG
                     SAMLEQIAMSDRYKLWVDTCSEIFGGLDICAVEALHGKDGRDHIIEVVGSSMPLIGDHQ
                     DEDKQLIVELVVNKMTQALPRQPQRDASPGRGSHSQSSSPGALTLGRQTSQQPAGPPAQ
                     QRPPPQGGPPQPGPGPQRQGPPLQQRPPPQGQQHLSGLGPPAGSPLPQRLPSPTAAPQQ
                     SASQATPVTQGQGRQSRPVAGGPGAPPAARPPASPSPQRQAGAPQATRQASISGPAPTK
                     ASGAPPGGQQRQGPPQKPPGPAGPTRQASQAGPGPRTGPPTTQQPRPSGPGPAGRPAKP
                     QLAQKPSQDVPPPITAAAGGPPHPQLNKSQSLTNAFNLPEPAPPRPSLSQDEVKAETIR
                     SLRKSFASLFSD"
     protein_bind    complement(5323..5356)
                     /label=lox2272
     primer_bind     5368..5457
                     /label=AJ18023
     protein_bind    complement(5407..5440)
                     /label=loxP
     primer_bind     complement(5441..5469)
                     /label=AJ19027
     misc_feature    5476..6064
                     /label=WPRE
                     /note="woodchuck posttranscriptional regulatory element"
     CDS             complement(5947..5958)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6067..6083)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             6589..6769
                     /label=5' LTR
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6801..7025
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7071..7499
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7513..7842
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7693..7828
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7890..7937
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7956..8330
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8460..8581
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8630..8646)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8654..8670
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8678..8708)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8723..8744
                     /label=CAP bindin
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site;CAP binding activates transcription
                     in the presence of cAMP."
     rep_origin      complement(9032..9620)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9791..10651)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10652..10756)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
       61 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
      121 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
      181 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      241 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      301 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      361 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      421 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      481 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      541 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      601 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      661 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      721 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
      781 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      841 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      901 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      961 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     1021 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     1081 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     1141 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     1201 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     1261 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     1321 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     1381 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     1441 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     1501 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1561 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1621 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1681 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1741 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1801 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1861 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1921 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1981 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     2041 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     2101 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     2161 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     2221 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     2281 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2341 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     2401 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2461 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2521 tacagggaca gcagagatcc agtttggtta attaactgca gagggccctg cgtatgagtg
     2581 caagtgggtt ttaggaccag gatgaggcgg ggtgggggtg cctacctgac gaccgacccc
     2641 gacccactgg acaagcaccc aacccccatt ccccaaattg cgcatcccct atcagagagg
     2701 gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc accgcggaca
     2761 gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga aggcgcgctg
     2821 acgtcactcg ccggtccccc gcaaactccc cttcccggcc accttggtcg cgtccgcgcc
     2881 gccgccggcc cagccggacc gcaccacgcg aggcgcgaga taggggggca cgggcgcgac
     2941 catctgcgct gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg cagcggagga
     3001 gtcgtgtcgt gcctgagagc gcagtctaga ataacttcgt ataggatact ttatacgaag
     3061 ttatgcagaa tggtagctgg attgtagctg ctattagcaa tatgaaacct cttaataact
     3121 tcgtatagca tacattatac gaagttatac tagaccggtc tacttgtcat cgtcgtcttt
     3181 atagtctgaa ccagatccgt cggagaagag gctggcgaaa gacttcctca ggctgcggat
     3241 ggtctcagct ttcacctcgt cctggctaag gctgggcctg ggaggggctg gctctggaag
     3301 gttgaaggca ttggtcagag actgggattt gttgagctgg gggtgcgggg gtcccccggc
     3361 agcggcggtg atgggtggtg gcacatcctg gctgggtttc tgggccagct gtggtttggc
     3421 gggacgtcca gcaggacctg ggccgctggg ccggggctgc tgtgtggtgg gaggcccagt
     3481 gcgaggtccg ggacctgcct gactggcctg acgagtggga ccagcagggc ctgggggttt
     3541 ttggggaggg ccctggcgct gctgccctcc gggtggggct cctgaggcct tcgttggagc
     3601 tggaccagag atagatgcct gacgggtagc ctgcggggcc cccgcctgac gctgtggaga
     3661 tggggaggct ggtgggcgcg ctgctggagg tgctccaggg cctcctgcca ctggccgcga
     3721 ctggcggcct tgaccctggg tcactggtgt ggcctgagag gcagactgct gaggtgctgc
     3781 ggtgggactt ggtaggcgct gaggcagagg gctgccagct ggcggtccaa ggccagaaag
     3841 atgttgctgg ccttgtgggg gtgggcgctg ctgcagcggg ggtccctggc gctgaggtcc
     3901 tgggcctggc tgtggagggc ctccctgggg tgggggtcgt tgttgagcag gaggacctgc
     3961 aggctgctgg gaggtctggc ggcccaaggt cagggctcct ggggatgaag actggctgtg
     4021 ggagcccctg ccaggggaag catcccgctg cggctgccga ggcagagcct gagtcatctt
     4081 gttgaccaca agttccacga tgagctgctt gtcttcatcc tggtgatcac caatgagtgg
     4141 catggaggag cccaccacct caataatgtg atcccttccg tccttgccat gcagcgcttc
     4201 cactgcgcag atgtcaagtc ccccaaaaat ctctgagcac gtgtctaccc acaacttgta
     4261 cctgtcagac atggcaatct gctcaagcat agcagaacct gtattggtct tccagttacc
     4321 cgacactgat gtcctcatgt aggccttgta gttctgccca atcttctgga cacgcacatc
     4381 atatttagca tcaatgaagg gctcagcagt ggcatatgtc ttagtcagtg ccacaacact
     4441 tgcaatatcc tggaagtcat gttggttgtc taccttgacc ttgcccatcc cagaatgtgc
     4501 gtggcccatc ttcacaacca cagggtatgt tgtgctgctg agcatctctt tgtgattagg
     4561 atagaaagtc tgatcaatca gagggaattc ctctgttcca agcttcttgt gtagtcgaac
     4621 catctgggca aacacccagg gtttgtcaca gaagttgtag acagaatgca aagagtttac
     4681 actggggatt ccagcatact gcagcccaat gaccaaactt cggtagtctc cattacgtgc
     4741 catgctgaag gcatgctggc ggatcagcac aaagtctggc ttcagagacc tcacaacttt
     4801 gactccattc cgaagaactt ccatgtccac agagaatcca ccattggcat gagccacaag
     4861 attgagatca gagaattcag cttgctctac tttaatgtca atttctccat ggatcttctt
     4921 ccctttgaag tattttgccc agtcggtgtg cggttcgtcg atgaccagca gcaccctggc
     4981 ggcggcgccc ccgcggcctg cgcccccaga gccaccgccc acctgctcgc tgaaggtggc
     5041 ggctgcggct gctgtggttt gcttgaccgc gttagacagc gacgagaaga agccgccgcc
     5101 ccccgaggac ccaggactag gggctgctgg agaagccact ggagcagctg tggaggccct
     5161 ctcggcagag gctgtcgcgg agccgggcgt ggccccaggg ctggcggccg agggaggcgg
     5221 cgggggcggt tgcgggcgct gcaggtctgt catgtaccca ttcggcagat tggccatgaa
     5281 gttgctgtcc gacaggcggc gccgcaggta gttcatgcta gcataacttc gtataaagta
     5341 tcctatacga agttatttgc cttaacccag aaattatcac tgttattctt tagaatggtg
     5401 caaagaataa cttcgtataa tgtatgctat acgaagttat ggatccgtta acaattcgat
     5461 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     5521 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     5581 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     5641 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     5701 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     5761 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     5821 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     5881 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     5941 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     6001 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     6061 ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
     6121 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     6181 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     6241 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     6301 gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac
     6361 acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca
     6421 gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct
     6481 gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc
     6541 cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg
     6601 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6661 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     6721 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta
     6781 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     6841 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     6901 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     6961 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     7021 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     7081 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     7141 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     7201 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     7261 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     7321 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     7381 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     7441 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     7501 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     7561 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     7621 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     7681 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7741 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     7801 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     7861 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
     7921 gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc
     7981 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
     8041 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
     8101 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
     8161 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
     8221 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
     8281 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac
     8341 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     8401 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     8461 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     8521 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     8581 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     8641 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     8701 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     8761 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8821 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     8881 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8941 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     9001 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     9061 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     9121 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     9181 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     9241 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     9301 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     9361 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     9421 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     9481 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     9541 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     9601 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9661 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9721 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9781 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9841 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9901 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9961 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    10021 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    10081 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    10141 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    10201 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    10261 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    10321 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    10381 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    10441 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    10501 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    10561 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    10621 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    10681 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10741 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacacgaca c
//
