LOCUS       Exported               13160 bp ds-DNA     circular SYN 29-JAN-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13160)
  AUTHORS   Birchler Lab
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 29, 2020 from SnapGene 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13160
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(114..136)
                     /label=RBO-R
                     /note="color: black; sequence: GTCGCGTAACTTAGGACTTGTGC"
     primer_bind     complement(114..136)
                     /label=3'RBO - BOB
                     /note="color: black; sequence: gtcgcgtaacttaggacttgtgc"
     CDS             1217..1846
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /label=pVS1 StaA
                     /note="color: #993366"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2280..3347
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid 
                     pVS1"
                     /label=pVS1 RepA
                     /note="color: #993366"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     misc_feature    3951..4091
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
     rep_origin      complement(4277..4865)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(5109..5900)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /note="color: #ccffcc"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     6090..6112
                     /label=5'LBO F - BOB
                     /note="color: black; sequence: cggtcaaggttctggaccagttg"
     primer_bind     6360..6379
                     /label=LBO - BOB
                     /note="color: black; sequence: gatgggctgcctgtatcgag"
     misc_feature    6428..6452
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     primer_bind     6477..6496
                     /label=pZY101-LB-1 (604) F
                     /note="color: black; sequence: aacacattgcggacgttttt"
     primer_bind     6505..6524
                     /label=pTF52fromLB
                     /note="color: black; sequence: gaattaacgccgaattgctc"
     primer_bind     6528..6539
                     /label=PZY101 Bkbone R
                     /note="color: black; sequence: ctcaagctcattcgccattc"
     primer_bind     6575..6593
                     /label=V2 puc8-Ubi-FLPe-NOS R 2
                     /note="color: black; sequence: ggcctcttcgctattacgc"
     primer_bind     complement(6613..6633)
                     /label=5'RBO F - BOB
                     /note="color: black; sequence: aacttaatcgccttgcagcac"
     primer_bind     6640..6663
                     /label=M13-23merF
                     /note="color: black; sequence: cgccagggttttcccagtcacgac"
     primer_bind     6642..6660
                     /label=V2 puc8-Ubi-FLPe-NOS R
                     /note="color: black; sequence: ccagggttttcccagtcac"
     primer_bind     6647..6666
                     /label=FLPe Screen R
                     /note="color: black; sequence: gttttcccagtcacgacgtt"
     primer_bind     6647..6663
                     /label=M13-17merF
                     /note="color: black; sequence: gttttcccagtcacgac"
     primer_bind     6898..6917
                     
                     /note="color: black; sequence: agacgacgtcgattttctgc"
     primer_bind     complement(7061..7080)
                     
                     /note="color: black; sequence: aatcttctgcccttgttcca"
     CDS             complement(7251..7801)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /note="color: #ccffcc"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     primer_bind     7285..7303
                     /label=FRT1 Scr R
                     /note="color: black; sequence: GTACCGGCAGGCTGAAGTC"
     primer_bind     complement(7429..7448)
                     /label=BarEndF
                     /note="color: black; sequence: CTTCAAGAGCGTGGTCGCTG"
     primer_bind     7475..7495
                     /label=BarNosInsRa
                     /note="color: black; sequence: GTGGGTGTAGAGCGTGGAGCC"
     primer_bind     7475..7495
                     /label=BarNosInsR
                     /note="color: black; sequence: gtgggtgtagagcgtggagcc"
     primer_bind     7481..7499
                     /label=Bar35S (559) F
                     /note="color: black; sequence: gtagagcgtggagcccagt"
     primer_bind     complement(7545..7563)
                     /label=pZY101-LB-2 (666) R
                     /note="color: black; sequence: gcaacgcctacgactggac"
     primer_bind     complement(7583..7603)
                     /label=BarMidF
                     /note="color: black; sequence: GAGGTCGCCGGCATCGCCTAC"
     primer_bind     7724..7742
                     /label=FLPe Screen F
                     /note="color: black; sequence: gttgacgatggtgcagacc"
     primer_bind     complement(7788..7804)
                     /label=BAMHI-FRT-BAR-NOS F
                     /note="color: black; sequence: 
                     TCAAGGATCCAGCCAGAAGTTCCTATTCtctagaaaGTATAGGAACTTCGTAACCATGA
                     GCCCAGAACG"
     promoter        complement(7953..8297)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     7997..8016
                     /label=35S-Ubi (698) F
                     /note="color: black; sequence: ttgcgaaggatagtgggatt"
     primer_bind     complement(8020..8039)
                     /label=Bar35S (559) R
                     /note="color: black; sequence: cactgacgtaagggatgacg"
     primer_bind     8299..8318
                     /label=puc8-Ubi-FLPe-NOS F
                     /note="color: black; sequence: ttgccctttggtcttctgag"
     primer_bind     8302..8325
                     /label=ptf101TeloF
                     /note="color: black; sequence: ccctttggtcttctgagactgtat"
     primer_bind     complement(8338..8357)
                     /label=IntegraseProbe1R
                     /note="color: black; sequence: cgacacgcttgtctactcca"
     primer_bind     8343..8362
                     /label=V2 puc8-Ubi-FLPe-NOS F
                     /note="color: black; sequence: tagacaagcgtgtcgtgctc"
     primer_bind     8343..8362
                     /label=PZY101 MCS F
                     /note="color: black; sequence: tagacaagcgtgtcgtgctc"
     promoter        8395..10391
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     8426..8446
                     /label=Ubi1F
                     /note="color: black; sequence: CTCTAGAGATAATGAGCATTG"
     primer_bind     8441..8458
                     /label=IntegraseProbe2F
                     /note="color: black; sequence: gcgcattgcatgtctaagtt"
     primer_bind     8483..8504
                     /label=Ubi1F1
                     /note="color: black; sequence: TTGTCACACTTGTTTGAAGTGC"
     primer_bind     8612..8635
                     /label=Ubi-1 (619) F
                     /note="color: black; sequence: GACATGGTCTAAAGGACAATTGAG"
     primer_bind     8641..8660
                     /label=Ubi2-F
                     /note="color: black; sequence: TGACAACAGGACTCTACAGT"
     primer_bind     complement(8671..8694)
                     /label=UbiPR1
                     /note="color: black; sequence: AAAAAGGAGAACACATGCACACTA"
     primer_bind     complement(8674..8694)
                     /label=35S-Ubi (698) R
                     /note="color: black; sequence: AAAAAGGAGAACACATGCACA"
     primer_bind     8996..9017
                     /label=Contig2Rvs2
                     /note="color: black; sequence: GAGTCTAACGGACACCAACCAG"
     primer_bind     9125..9144
                     /label=Contig2Rvs1
                     /note="color: black; sequence: CTGTCGGCATCCAGAAATTG"
     primer_bind     9182..9200
                     /label=Ubi-2 (435) F
                     /note="color: black; sequence: GGCCTCCTCCTCCTCTCAC"
     primer_bind     complement(9212..9230)
                     /label=Ubi-1 (619) R
                     /note="color: black; sequence: GGAAAGGAATCCCCCGTAG"
     primer_bind     complement(9333..9352)
                     /label=IntegraseProbe2R2
                     /note="color: black; sequence: gatttgggggagatctggtt"
     primer_bind     9446..9465
                     /label=Ubi1063F
                     /note="color: black; sequence: GGGCCCGGTAGTTCTACTTC"
     primer_bind     complement(9550..9572)
                     /label=Contig6Fwd1
                     /note="color: black; sequence: ACTGGCAAGTTAGCAATCAGAAC"
     primer_bind     complement(9568..9587)
                     /label=IntegraseProbe2R
                     /note="color: black; sequence: tccccaaagagaaacactgg"
     primer_bind     9585..9604
                     /label=Ubi-3 (430) F
                     /note="color: black; sequence: GGAATCCTGGGATGGCTCTA"
     primer_bind     complement(9615..9634)
                     /label=Ubi-2 (453) R
                     /note="color: black; sequence: TCATGAAATCGATCCCGTCT"
     primer_bind     9647..9666
                     /label=IntegraseProbe3F
                     /note="color: black; sequence: cgttgcatagggtttggttt"
     primer_bind     9709..9728
                     /label=Primer3Forward1
                     /note="color: black; sequence: CGGGTCATCTTTTCATGCTT"
     primer_bind     complement(9827..9846)
                     /label=Contig4Fwd3
                     /note="color: black; sequence: TGACACACACATACAGTTCCAAA"
     primer_bind     9952..9971
                     /label=Ubi-4 (448) F
                     /note="color: black; sequence: TTTTGTTCGCTTGGTTGTGA"
     primer_bind     complement(9960..9979)
                     /label=Ubi1592R
                     /note="color: black; sequence: CCACATCATCACAACCAAGC"
     primer_bind     complement(9994..10014)
                     /label=Ubi-3 (430) R
                     /note="color: black; sequence: CCGATCTAGAACGAATGAACG"
     primer_bind     complement(10055..10077)
                     /label=Contig4Fwd3
                     /note="color: black; sequence: TGACACACACATACAGTTCCAAA"
     primer_bind     10058..10083
                     /label=Ubi1660F
                     /note="color: black; sequence: GGAACTGTATGTGTGTGTCATACATC"
     primer_bind     10357..10380
                     /label=Ubi47-F
                     /note="color: black; sequence: GATGCTCACCCTGTTGTTTGGTGT"
     primer_bind     10357..10376
                     /label=Ubi-Flpe (574) F
                     /note="color: black; sequence: GATGCTCACCCTGTTGTTTG"
     primer_bind     complement(10378..10399)
                     /label=Ubi-4 (448) R
                     /note="color: black; sequence: GAGTCGACCTGCAGAAGTAACA"
     primer_bind     complement(10379..10400)
                     /label=Ubi3'R
                     /note="color: black; sequence: AGAGTCGACCTGCAGAAGTAAC"
     CDS             10421..11689
                     /codon_start=1
                     /label=FLPe
                     /note="color: #3366ff"
                     /translation="MSQFDILCKTPPKVLVRQFVERFERPSGEKIASCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYNGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYPIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     primer_bind     10454..10473
                     /label=FLPe F
                     /note="color: black; sequence: CCTAAGGTCCTGGTTCGTCA"
     primer_bind     10866..10885
                     /label=Flpe (560) F
                     /note="color: black; sequence: GTGAGGGTGAAAGCATCTGG"
     primer_bind     complement(10906..10930)
                     /label=Ubi-Flpe (574) R
                     /note="color: black; sequence: TCTCGAGGTATACTCAAACGAATTT"
     primer_bind     11369..11388
                     /label=Flpe-NOS1 (797) F
                     /note="color: black; sequence: ATGAAGGGCCTAACGGAGTT"
     primer_bind     complement(11406..11425)
                     /label=Flpe (560) R
                     /note="color: black; sequence: AGCACGCTTATCGCTCCAAT"
     primer_bind     11622..11641
                     /label=FLPe Right F
                     /note="color: black; sequence: CCGCATGGAATGGGATAATA"
     primer_bind     complement(11624..11644)
                     /label=FLPe R
                     /note="color: black; sequence: TGATATTATCCCATTCCATGC"
     primer_bind     complement(11803..11822)
                     /label=FLPe Seq R
                     /note="color: black; sequence: AACGTGGGTAGCACCAAAAC"
     primer_bind     11867..11882
                     /label=AscI-AttP-NOS F
                     /note="color: black; sequence: 
                     GAATGGCGCGCCAATGACCCCAACTGGGGTAACCTTTGAGTTCTCTCAGTTGGGGGAAC
                     CTGCAGATCGTTCAAACA"
     terminator      11870..12122
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
                     /note="color: #ffffff"
     primer_bind     11872..11891
                     /label=BarNosSeq2.1
                     /note="color: black; sequence: TCGTTCAAACATTTGGCAAT"
     primer_bind     complement(11908..11926)
                     /label=Contig4Fwd1
                     /note="color: black; sequence: AAGACCGGCAACAGGATTC"
     primer_bind     complement(12012..12034)
                     /label=Contig4Fwd2
                     /note="color: black; sequence: TTGCGGGACTCTAATCATAAAAA"
     primer_bind     12018..12041
                     /label=BarNosSeq3.1
                     /note="color: black; sequence: TGATTAGAGTCCCGCAATTATACA"
     primer_bind     complement(12057..12077)
                     /label=NosTerm238R
                     /note="color: black; sequence: TTGCGCGCTATATTTTGTTTT"
     primer_bind     complement(12146..12165)
                     /label=Flpe-NOS1 (797) R
                     /note="color: black; sequence: CTCTCGATTGCTCATCGTCA"
     primer_bind     12461..12478
                     /label=NOS2-LB (465) F
                     /note="color: black; sequence: AcCCGAGCTCGAatTTCC"
     terminator      12481..12733
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
                     /note="color: #ffffff"
     primer_bind     12481..12493
                     /label=AscI-AttP-NOS F
                     /note="color: black; sequence: 
                     GAATGGCGCGCCAATGACCCCAACTGGGGTAACCTTTGAGTTCTCTCAGTTGGGGGAAC
                     CTGCAGATCGTTCAAACA"
     primer_bind     12483..12502
                     /label=BarNosSeq2.1
                     /note="color: black; sequence: TCGTTCAAACATTTGGCAAT"
     primer_bind     complement(12519..12537)
                     /label=Contig4Fwd1
                     /note="color: black; sequence: AAGACCGGCAACAGGATTC"
     primer_bind     complement(12623..12645)
                     /label=Contig4Fwd2
                     /note="color: black; sequence: TTGCGGGACTCTAATCATAAAAA"
     primer_bind     12629..12652
                     /label=BarNosSeq3.1
                     /note="color: black; sequence: TGATTAGAGTCCCGCAATTATACA"
     primer_bind     complement(12668..12688)
                     /label=NosTerm238R
                     /note="color: black; sequence: TTGCGCGCTATATTTTGTTTT"
     primer_bind     complement(12906..12925)
                     /label=NOS2-LB (465) R
                     /note="color: black; sequence: ATTAATGCAGCTGGCACGAC"
     primer_bind     complement(12977..12996)
                     /label=puc8-Ubi-FLPe-NOS R
                     /note="color: black; sequence: gggaaacgacaatctgatcc"
     primer_bind     complement(12991..13010)
                     /label=puc8-Ubi-FLPe-NOS R 2
                     /note="color: black; sequence: tttaaactgaaggcgggaaa"
     primer_bind     complement(12991..13010)
                     /label=IntegraseProbe4R
                     /note="color: black; sequence: tttaaactgaaggcgggaaa"
     misc_feature    13022..13046
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     primer_bind     complement(13086..13105)
                     /label=PZY101 MCS R
                     /note="color: black; sequence: aaaccttttcacgccctttt"
     primer_bind     complement(13122..13142)
                     /label=RBO - BOB
                     /note="color: black; sequence: ctgtggttggcatgcacatac"
ORIGIN
        1 agtactttaa agtactttaa agtactttaa agtactttga tccaacccct ccgctgctat
       61 agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag
      121 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt
      181 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag
      241 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc
      301 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc
      361 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac
      421 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt
      481 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
      541 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
      601 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
      661 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
      721 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
      781 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
      841 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
      901 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
      961 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct
     1021 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
     1081 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     1141 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
     1201 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     1261 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     1321 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     1381 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     1441 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     1501 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     1561 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     1621 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     1681 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     1741 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     1801 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     1861 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     1921 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     1981 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     2041 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
     2101 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     2161 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     2221 ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgacg
     2281 gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga
     2341 agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg
     2401 aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg
     2461 gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga
     2521 tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc
     2581 tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg
     2641 tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga
     2701 tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc
     2761 ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg
     2821 gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg
     2881 ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag
     2941 ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga
     3001 tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga
     3061 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg
     3121 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca
     3181 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa
     3241 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca
     3301 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga
     3361 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata
     3421 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc
     3481 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag
     3541 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct
     3601 gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt
     3661 cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa
     3721 aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac
     3781 tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa
     3841 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     3901 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     3961 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     4021 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     4081 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4141 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4201 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4261 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4321 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4381 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4441 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4501 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4561 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4621 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4681 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     4741 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4801 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4861 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4921 gttaagggat tttggtcatg catgatatat ctcccaattt gtgtagggct tattatgcac
     4981 gcttaaaaat aataaaagca gacttgacct gatagtttgg ctgtgagcaa ttatgtgctt
     5041 agtgcatcta atcgcttgag ttaacgccgg cgaagcggcg tcggcttgaa cgaatttcta
     5101 gctagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg gacaaattct
     5161 tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata agcctgtcta
     5221 gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg
     5281 acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc
     5341 actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt taaggtttca
     5401 tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga
     5461 cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg
     5521 atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc
     5581 agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact
     5641 tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg
     5701 atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata
     5761 tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac
     5821 gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg
     5881 gcgatcaccg cttcccccat gatgtttaac tttgttttag ggcgactgcc ctgctgcgta
     5941 acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg
     6001 gatgcccgag gcatagactg taccccaaaa aaacatgtca taacaagaag ccatgaaaac
     6061 cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgacgg
     6121 cagttacgct acttgcatta cagcttacga accgaacgag gcttatgtcc actgggttcg
     6181 tgcccgaatt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc
     6241 cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg
     6301 gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg
     6361 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc
     6421 tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca
     6481 cattgcggac gtttttaatg tactgaatta acgccgaatt gctctagcat tcgccattca
     6541 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     6601 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     6661 gacgttgtaa aacgacggcc agtgccaagc taattcgctt caagacgtgc tcaaatcact
     6721 atttccacac ccctatattt ctattgcact cccttttaac tgttttttat tacaaaaatg
     6781 ccctggaaaa tgcactccct ttttgtgttt gtttttttgt gaaacgatgt tgtcaggtaa
     6841 tttatttgtc agtctactat ggtggcccat tatattaata gcaactgtcg gtccaataga
     6901 cgacgtcgat tttctgcatt tgtttaacca cgtggatttt atgacatttt atattagtta
     6961 atttgtaaaa cctacccaat taaagacctc atatgttcta aagactaata cttaatgata
     7021 acaattttct tttagtgaag aaagggataa ttagtaaata tggaacaagg gcagaagatt
     7081 tattaaagcc gcgtaagaga caacaagtag gtacgtggag tgtcttaggt gacttaccca
     7141 cataacataa agtgacatta acaaacatag ctaatgctcc tatttgaata gtgcatatca
     7201 gcatacctta ttacatatag ataggagcaa actctagcta gattgttgag cagatctcgg
     7261 tgacgggcag gaccggacgg ggcggtaccg gcaggctgaa gtccagctgc cagaaaccca
     7321 cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag catgccgcgg ggggcatatc
     7381 cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca gcgaccacgc
     7441 tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg gagcccagtc
     7501 ccgtccgctg gtggcggggg gagacgtaca cggtcgactc ggccgtccag tcgtaggcgt
     7561 tgcgtgcctt ccaggggccc gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga
     7621 cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct
     7681 gcggctcggt acggaagttg accgtgcttg tctcgatgta gtggttgacg atggtgcaga
     7741 ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt tctgggctca
     7801 tggtagatcc cccgttcgta aatggtgaaa attttcagaa aattgctttt gctttaaaag
     7861 aaatgattta aattgctgca atagaagtag aatgcttgat tgcttgagat tcgtttgttt
     7921 tgtatatgtt gtgttgagaa ttaattctcg aggtcctctc caaatgaaat gaacttcctt
     7981 atatagagga agggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg
     8041 agatatcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga
     8101 tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttcaacga
     8161 tggcctttcc tttatcgcaa tgatggcatt tgtaggagcc accttccttt tccactatct
     8221 tcacaataaa gtgacagata gctgggcaat ggaatccgag gaggtttccg gatattaccc
     8281 tttgttgaaa agtctcaatt gccctttggt cttctgagac tgtatctttg atatttttgg
     8341 agtagacaag tgtgtcgtgc tccaccatgt tgaccggcat gcaagcttgc atgcctgcag
     8401 tgcagcgtga cccggtcgtg cccctctcta gagataatga gcattgcatg tctaagttat
     8461 aaaaaattac cacatatttt ttttgtcaca cttgtttgaa gtgcagttta tctatcttta
     8521 tacatatatt taaactttac tctacgaata atataatcta tagtactaca ataatatcag
     8581 tgttttagag aatcatataa atgaacagtt agacatggtc taaaggacaa ttgagtattt
     8641 tgacaacagg actctacagt tttatctttt tagtgtgcat gtgttctcct ttttttttgc
     8701 aaatagcttc acctatataa tacttcatcc attttattag tacatccatt tagggtttag
     8761 ggttaatggt ttttatagac taattttttt agtacatcta ttttattcta ttttagcctc
     8821 taaattaaga aaactaaaac tctattttag tttttttatt taataattta gatataaaat
     8881 agaataaaat aaagtgacta aaaattaaac aaataccctt taagaaatta aaaaaactaa
     8941 ggaaacattt ttcttgtttc gagtagataa tgccagcctg ttaaacgccg tcgacgagtc
     9001 taacggacac caaccagcga accagcagcg tcgcgtcggg ccaagcgaag cagacggcac
     9061 ggcatctctg tcgctgcctc tggacccctc tcgagagttc cgctccaccg ttggacttgc
     9121 tccgctgtcg gcatccagaa attgcgtggc ggagcggcag acgtgagccg gcacggcagg
     9181 cggcctcctc ctcctctcac ggcaccggca gctacggggg attcctttcc caccgctcct
     9241 tcgctttccc ttcctcgccc gccgtaataa atagacaccc cctccacacc ctctttcccc
     9301 aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa tccacccgtc
     9361 ggcacctccg cttcaaggta cgccgctcgt cctccccccc cccccctctc taccttctct
     9421 agatcggcgt tccggtccat ggttagggcc cggtagttct acttctgttc atgtttgtgt
     9481 tagatccgtg tttgtgttag atccgtgctg ctagcgttcg tacacggatg cgacctgtac
     9541 gtcagacacg ttctgattgc taacttgcca gtgtttctct ttggggaatc ctgggatggc
     9601 tctagccgtt ccgcagacgg gatcgatttc atgatttttt ttgtttcgtt gcatagggtt
     9661 tggtttgccc ttttccttta tttcaatata tgccgtgcac ttgtttgtcg ggtcatcttt
     9721 tcatgctttt ttttgtcttg gttgtgatga tgtggtctgg ttgggcggtc gttctagatc
     9781 ggagtagaat taattctgtt tcaaactacc tggtggattt attaattttg gatctgtatg
     9841 tgtgtgccat acatattcat agttacgaat tgaagatgat ggatggaaat atcgatctag
     9901 gataggtata catgttgatg cgggttttac tgatgcatat acagagatgc tttttgttcg
     9961 cttggttgtg atgatgtggt gtggttgggc ggtcgttcat tcgttctaga tcggagtaga
    10021 atactgtttc aaactacctg gtgtatttat taattttgga actgtatgtg tgtgtcatac
    10081 atcttcatag ttacgagttt aagatggatg gaaatatcga tctaggatag gtatacatgt
    10141 tgatgtgggt tttactgatg catatacatg atggcatatg cagcatctat tcatatgctc
    10201 taaccttgag tacctatcta ttataataaa caagtatgtt ttataattat tttgatcttg
    10261 atatacttgg atgatggcat atgcagcagc tatatgtgga tttttttagc cctgccttca
    10321 tacgctattt atttgcttgg tactgtttct tttgtcgatg ctcaccctgt tgtttggtgt
    10381 tacttctgca ggtcgactct agaggatccc ccgcgccacc atgagccaat ttgatatatt
    10441 atgtaaaaca ccacctaagg tcctggttcg tcagtttgtg gaaaggtttg aaagaccttc
    10501 aggggaaaaa atagcatcat gtgctgctga actaacctat ttatgttgga tgattactca
    10561 taacggaaca gcaatcaaga gagccacatt catgagctat aatactatca taagcaattc
    10621 gctgagtttc gatattgtca acaaatcact ccagtttaaa tacaagacgc aaaaagcaac
    10681 aattctggaa gcctcattaa agaaattaat tcctgcttgg gaatttacaa ttattcctta
    10741 caatggacaa aaacatcaat ctgatatcac tgatattgta agtagtttgc aattacagtt
    10801 cgaatcatcg gaagaagcag ataagggaaa tagccacagt aaaaaaatgc ttaaagcact
    10861 tctaagtgag ggtgaaagca tctgggagat cactgagaaa atactaaatt cgtttgagta
    10921 tacctcgaga tttacaaaaa caaaaacttt ataccaattc ctcttcctag ctactttcat
    10981 caattgtgga agattcagcg atattaagaa cgttgatccg aaatcattta aattagtcca
    11041 aaataagtat ctgggagtaa taatccagtg tttagtgaca gagacaaaga caagcgttag
    11101 taggcacata tacttcttta gcgcaagggg taggatcgat ccacttgtat atttggatga
    11161 atttttgagg aactctgaac cagtcctaaa acgagtaaat aggaccggca attcttcaag
    11221 caacaaacag gaataccaat tattaaaaga taacttagtc agatcgtaca acaaggcttt
    11281 gaagaaaaat gcgccttatc caatctttgc tataaagaat ggcccaaaat ctcacattgg
    11341 aagacatttg atgacctcat ttctgtcaat gaagggccta acggagttga ctaatgttgt
    11401 gggaaattgg agcgataagc gtgcttctgc cgtggccagg acaacgtata ctcatcagat
    11461 aacagcaata cctgatcact acttcgcact agtttctcgg tactatgcat atgatccaat
    11521 atcaaaggaa atgatagcat tgaaggatga gactaatcca attgaggagt ggcagcatat
    11581 agaacagcta aagggtagtg ctgaaggaag catacgatac cccgcatgga atgggataat
    11641 atcacaggag gtactagact acctttcatc ctacataaat agacgcatag gatgagctag
    11701 tgcatgcatt catcaatatt attcatgcgg ggaaaggcaa gattaatcca actggcaaat
    11761 catccagcgt gattggtaac ttcagttcca gcgacttgat tcgttttggt gctacccacg
    11821 ttttcaataa ggacgagatg gtggagtaaa gaaggagtgc gtcgaagcag atcgttcaaa
    11881 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
    11941 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
    12001 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
    12061 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
    12121 tcgatcaaac ttcggcactg tgtaatgacg atgagcaatc gagaggctga ctaacaaaag
    12181 gtatgcccaa aaacaacctc tccaaactgt ttcgaattgg aagtttctgc tcatgccgac
    12241 aggcataact tagatattcg cgggctattc ccactaattc gtcctgctgg tttgcgccaa
    12301 gataaatcag tgcatctcct tacaagttcc tctgtcttgt gaaatgaact gctgactgcc
    12361 ccccaagaaa gcctcctcat ctcccagttg gcggcggctg atacaccatc gaaaacccac
    12421 gtccgaacac ttgatacatg tgcctgagaa ataggggtcg acccgagctc gaatttcccc
    12481 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
    12541 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
    12601 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
    12661 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
    12721 atgttactag atcgggaatt cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat
    12781 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
    12841 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
    12901 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
    12961 attggagctt gagcttggat cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt
    13021 ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga
    13081 tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac
    13141 agggttcccc tcgggatcaa
//
