LOCUS       Exported                5498 bp ds-DNA     circular SYN 09-JAN-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Birchler Lab
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 29, 2020 from SnapGene 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     protein_bind    37..58
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        73..103
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1:  73 ..  78 / #ffffff / -35
                        2:  79 ..  96 / #ffffff
                        3:  97 .. 103 / #ffffff / -10"
     primer_bind     93..115
                     /label=ptf101TeloR2
                     /note="TM- 60.33 Sequence into Telo of ptf101.1 and 
                     derivatives from RB into EcoRI site"
                     /note="color: black; sequence: CTCGTATGTTGTGTGGAATTGTG"
     primer_bind     95..117
                     /label=ptf101TeloR
                     /note="TM-60.33 Sequence into Telo"
                     /note="color: black; sequence: CGTATGTTGTGTGGAATTGTGAG"
     primer_bind     104..123
                     /label=pGEMScreenR
                     /note="TM-60.07 Screen for pGEMT or Easy Inserts 163 to 
                     Insert (Easy)"
                     /note="color: black; sequence: TGTGGAATTGTGAGCGGATA"
     primer_bind     104..123
                     /label=topo2.1ScreenF
                     /note="TM-60.07 Sequence across topo insert 232 without 
                     insert"
                     /note="color: black; sequence: TGTGGAATTGTGAGCGGATA"
     protein_bind    111..127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     130..151
                     /label=m13R-23mer
                     /note="color: black; sequence: TCACACAGGAAACAGCTATGAC"
     primer_bind     135..151
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     135..151
                     /label=m13R-17mer
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     promoter        172..190
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    complement(202)
                     /label=Below threshold
                     /note="This reverse directional feature has 3 segments:
                        1:  202 .. 4483 / #a6acb3
                        2: 4484 .. 4485 / #------
                        3: 4486 ..  202 / #a6acb3"
     misc_feature    complement(202)
                     /label=Contig 2(1&gt;4774)
                     /note="This reverse directional feature has 3 segments:
                        1:  202 .. 4483 / #a6acb3
                        2: 4484 .. 4485 / #------
                        3: 4486 ..  202 / #a6acb3"
     misc_feature    complement(203..2303)
                     /label=Contig 2(1&gt;4774)
                     /note="color: #0000ff; direction: LEFT"
     misc_feature    complement(203..1415)
                     /label=Below threshold
                     /note="color: #625fff; direction: LEFT"
     misc_feature    complement(203..1073)
                     /label=One_strand
                     /note="color: #ff0000; direction: LEFT"
     misc_feature    203..324
                     /label=pMECA
                     /note="pMECA"
                     /note="color: #0080ff"
     primer_bind     227..245
                     /label=pMECARvsBsaAI
                     /note="TM-60.13 Primer Rvs in pMECA BsaAI-BssHii"
                     /note="color: black; sequence: cgcgccagatctagacgta"
     primer_bind     227..245
                     /label=pMECAScreen2
                     /note="TM-59.1 Screen for pMECA insert into pNG2 107bp to 
                     FSE1"
                     /note="color: black; sequence: cgcgccagatctagacgta"
     primer_bind     234..255
                     /label=pMECAScreen1
                     /note="TM-60 Screen for pMECA insert into pNG2 100bp to 
                     FSE1"
                     /note="color: black; sequence: gatctagacgtacgtggccaat"
     primer_bind     276..298
                     /label=HindIII-Ubi
                     /note="Tm-60.7 2374bp with SacI-Nos Insert Ubi Nos into 
                     pTF"
                     /note="color: black; sequence: 
                     GATGATTAAAGCTTaCGTTTAAACAGTCGACGTCGCCC"
     primer_bind     complement(280..298)
                     /label=pMECAControl64
                     /note="TM-58 Screen for pMECA insert into pNG2 65bp Product
                     with Screen1, only for use with pWY82-Full"
                     /note="color: black; sequence: gggcgacgtcgactgttta"
     primer_bind     307..319
                     /label=UbiFullNoHINDIII
                     /note="TM-61.15 Full Ubi No HINDIII"
                     /note="color: black; sequence: GCTTGCATGCCTGCAGTGCAG"
     primer_bind     309..326
                     /label=HIIInoBUbi56GFP-F
                     /note="Amplify Ubi-EGFP-Nos from p56EGFP to insert into 
                     pZY101"
                     /note="color: black; sequence: 
                     GCTTAAAGCTTATTATGCAGTGCAGCGTGACC"
     promoter        310..2300
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     322..341
                     /label=IntExc_fwd
                     /note="TM-62.1 NEBuilder primers for LongampTaq"
                     /note="color: black; sequence: 
                     caggcatgcaagctcaTGACCCGGTCGTGCCCCTCT"
     primer_bind     340..360
                     /label=Ubi1F
                     /note="color: black; sequence: CTCTAGAGATAATGAGCATTG"
     primer_bind     355..372
                     /label=IntegraseProbe2F
                     /note="Tm-58.95 First Half of Ubiquitin"
                     /note="color: black; sequence: gcgcattgcatgtctaagtt"
     primer_bind     397..418
                     /label=Ubi1F1
                     /note="color: black; sequence: TTGTCACACTTGTTTGAAGTGC"
     primer_bind     397..418
                     /label=UbiF1
                     /note="TM-58.88 Primer of UBI before primer begins on GZUS"
                     /note="color: black; sequence: TTGTCACACTTGTTTGAAGTGC"
     primer_bind     399..420
                     /label=UbiF2
                     /note="TM-58.48 Primer of UBI before primer begins on GZUS"
                     /note="color: black; sequence: GTCACACTTGTTTGAAGTGCAG"
     primer_bind     555..574
                     /label=Ubi2-F
                     /note="color: black; sequence: TGACAACAGGACTCTACAGT"
     primer_bind     complement(585..608)
                     /label=UbiPRev1
                     /note="color: black; sequence: AAAAAGGAGAACACATGCACACTA"
     primer_bind     587..608
                     /label=pTFPhiF1
                     /note="TM-59.15 Amplify ubi-int-nos for probe (Not full)"
                     /note="color: black; sequence: gtgtgcatgtgttctccttttt"
     primer_bind     587..608
                     /label=pTFPhiF2
                     /note="TM-59.15 Amplify ubi-int-nos for probe (Not full)"
                     /note="color: black; sequence: gtgtgcatgtgttctccttttt"
     primer_bind     910..931
                     /label=Contig2Rvs2
                     /note="TM-60.96"
                     /note="color: black; sequence: GAGTCTAACGGACACCAACCAG"
     primer_bind     1039..1058
                     /label=Contig2Rvs1
                     /note="TM-61.57"
                     /note="color: black; sequence: CTGTCGGCATCCAGAAATTG"
     misc_feature    complement(1074..1301)
                     /label=Only_once
                     /note="color: #ffa552; direction: LEFT"
     primer_bind     complement(1247..1266)
                     /label=IntegraseProbe2R2
                     /note="Tm-60.1 First Half of Ubiquitin"
                     /note="color: black; sequence: gatttgggggagatctggtt"
     primer_bind     1360..1379
                     /label=Ubi1063F
                     /note="Bob Designed Primer"
                     /note="color: black; sequence: GGGCCCGGTAGTTCTACTTC"
     misc_feature    complement(1416..1417)
                     /label=Above threshold
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    complement(1418..2303)
                     /label=Below threshold
                     /note="color: #625fff; direction: LEFT"
     primer_bind     complement(1464..1486)
                     /label=Contig6Fwd1 (2)
                     /note="TM-59.84"
                     /note="color: black; sequence: ACTGGCAAGTTAGCAATCAGAAC"
     primer_bind     complement(1482..1501)
                     /label=Contig6Fwd1
                     /note="TM-60.08"
                     /note="color: black; sequence: TCCCCAAAGAGAAACACTGG"
     primer_bind     complement(1482..1501)
                     /label=IntegraseProbe2R
                     /note="TM-60.08 First Half of Ubiquitin"
                     /note="color: black; sequence: tccccaaagagaaacactgg"
     misc_feature    complement(1529..1658)
                     /label=Only_once
                     /note="color: #ffa552; direction: LEFT"
     primer_bind     1561..1580
                     /label=IntegraseProbe3F
                     /note="TM-59.9 Second Half Ubiquitin into Integrase"
                     /note="color: black; sequence: cgttgcatagggtttggttt"
     primer_bind     1561..1580
                     /label=UbiIntF2
                     /note="TM-59.86 Ubi Forward Primer designed for making INT 
                     Fish Probe"
                     /note="color: black; sequence: cgttgcatagggtttggttt"
     primer_bind     1623..1642
                     /label=P3F1
                     /note="TM-60 Sequence from back half of Ubi"
                     /note="color: black; sequence: CGGGTCATCTTTTCATGCTT"
     primer_bind     1623..1642
                     /label=UbiIntF1
                     /note="TM-60.07 Ubi Forward Primer designed for making INT 
                     Fish Probe"
                     /note="color: black; sequence: cgggtcatcttttcatgctt"
     primer_bind     complement(1737..1756)
                     /label=Contig4Fwd3
                     /note="TM-59.01"
                     /note="color: black; sequence: TGACACACACATACAGTTCCAAA"
     primer_bind     complement(1870..1889)
                     /label=pWY56cUbiR
                     /note="TM-59.97"
                     /note="color: black; sequence: CCACATCATCACAACCAAGC"
     primer_bind     complement(1870..1889)
                     /label=pWY56cWalk1
                     /note="TM-59.97 Walk into pWY56c from end of NOS"
                     /note="color: black; sequence: CCACATCATCACAACCAAGC"
     primer_bind     complement(1870..1889)
                     /label=Ubi1592R
                     /note="Bob Designed Primer"
                     /note="color: black; sequence: CCACATCATCACAACCAAGC"
     primer_bind     complement(1965..1987)
                     /label=Contig4Fwd3
                     /note="TM-59.01"
                     /note="color: black; sequence: TGACACACACATACAGTTCCAAA"
     primer_bind     1968..1993
                     /label=Ubi1660F
                     /note="Bob Designed Primer"
                     /note="color: black; sequence: GGAACTGTATGTGTGTGTCATACATC"
     misc_feature    complement(2011..2123)
                     /label=DNA_JOIN
                     /note="joined contig Contig 2 with Contig 1"
                     /note="color: #0000ff; direction: LEFT"
     primer_bind     2267..2290
                     /label=Ubi47-F
                     /note="Bob Designed Primer"
                     /note="color: black; sequence: GATGCTCACCCTGTTGTTTGGTGT"
     misc_feature    2301..2303
                     /label=MCS
                     /note="MCS"
                     /note="color: #0080ff"
     protein_bind    2308..2341
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="loxP mutant. Cre-mediated recombination occurs in 
                     the 8-bp core sequence (GCATACAT)."
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    2308..2312
                     /label=Mutant 77 Site
                     /note="color: #ff0000"
     CDS             complement(2342..3019)
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     protein_bind    complement(3020..3053)
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="loxP mutant. Cre-mediated recombination occurs in 
                     the 8-bp core sequence (GCATACAT)."
                     /note="color: #31849b; direction: LEFT"
     misc_feature    3020..3024
                     /label=Mutant 66 Site
                     /note="color: #ff0000"
     misc_feature    complement(3055..4483)
                     /label=Contig 2(1&gt;4774)
                     /note="color: #0000ff; direction: LEFT"
     misc_feature    complement(3055..4483)
                     /label=Below threshold
                     /note="color: #625fff; direction: LEFT"
     misc_feature    3055..3079
                     /label=MCS
                     /note="MCS"
                     /note="color: #0080ff"
     terminator      3068..3320
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
                     /note="color: #ffffff"
     primer_bind     3070..3089
                     /label=BarNosSeq2.1
                     /note="TM-59.02"
                     /note="color: black; sequence: TCGTTCAAACATTTGGCAAT"
     primer_bind     complement(3071..3092)
                     /label=pTFPhiR1
                     /note="TM-59.88 Amplify ubi-int-nos for probe (Not full)"
                     /note="color: black; sequence: tttattgccaaatgtttgaacg"
     primer_bind     complement(3105..3124)
                     /label=NOSRev1
                     /note="TM-59.6 In Nos Reverse"
                     /note="color: black; sequence: AAGACCGGCAACAGGATTCA"
     primer_bind     complement(3106..3124)
                     /label=Contig4Fwd1
                     /note="TM-60.07"
                     /note="color: black; sequence: AAGACCGGCAACAGGATTC"
     misc_feature    complement(3210..3558)
                     /label=One_strand
                     /note="color: #ff0000; direction: LEFT"
     primer_bind     complement(3210..3232)
                     /label=Contig4Fwd2
                     /note="TM-59.98"
                     /note="color: black; sequence: TTGCGGGACTCTAATCATAAAAA"
     primer_bind     3216..3239
                     /label=BarnosSeq3.1
                     /note="TM-59.89"
                     /note="color: black; sequence: TGATTAGAGTCCCGCAATTATACA"
     primer_bind     3293..3311
                     /label=RandomPCRF1
                     /note="color: black; sequence: CGCGCGGTGTCATCTATGT"
     primer_bind     complement(3295..3326)
                     /label=IntExc_rev
                     /note="TM-62.1 NEBuilder primers for LongampTaq"
                     /note="color: black; sequence: 
                     gtacccggggatcctATTCCCGATCTAGTAACATAGATGACACCGCG"
     promoter        3343..3414
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     complement(3358..3381)
                     /label=SacI-Nos
                     /note="Tm-60.2 2374bp with HindII-Ubi"
                     /note="color: black; sequence: 
                     ATTGCATTCGAGCTCAGGGTTATTGTCTCATGAGCGG"
     CDS             3415..4275
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 3415 .. 3483 / #ccffcc / signal sequence
                        2: 3484 .. 4275 / #ccffcc
                     Cleavage site after base 3483"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    complement(3559..3706)
                     /label=Only_once
                     /note="color: #ffa552; direction: LEFT"
     primer_bind     3633..3652
                     /label=pWY56cBackbone F
                     /note="TM-59.98 Walk into pWY56c to connect contigs"
                     /note="color: black; sequence: GCTATGTGGCGCGGTATTAT"
     misc_feature    complement(3707..3888)
                     /label=One_strand
                     /note="color: #ff0000; direction: LEFT"
     misc_feature    complement(3889..4483)
                     /label=Only_once
                     /note="color: #ffa552; direction: LEFT"
     primer_bind     4201..4220
                     /label=pWY56cWalk2
                     /note="TM-60.07 Walk into pWY56c from pWY56cBackbone 
                     primer"
                     /note="color: black; sequence: GGGAGTCAGGCAACTATGGA"
     misc_feature    complement(4484..5257)
                     /label=Only_once
                     /note="color: #ffa552; direction: LEFT"
     rep_origin      4657..5247
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     5209..5228
                     /label=pBlueMCSR1
                     /note="TM-59.84 Sequence into MCS of pBluescriptIIKS+"
                     /note="color: black; sequence: TTTTTGTGATGCTCGTCAGG"
     misc_feature    complement(join(5258..5498,1..202))
                     /label=One_strand
                     /note="color: #ff0000; direction: LEFT"
     primer_bind     5404..5423
                     /label=pSP72R
                     /note="336"
                     /note="color: black; sequence: GAGTCAGTGAGCGAGGAAGC"
ORIGIN
        1 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
       61 ggcaccccag gctttacact ttatgctccc ggctcgtatg ttgtgtggaa ttgtgagcgg
      121 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
      181 tcactaaagg gaacaaaagc tgggtaccat ggatcgatcc tcgaggcgcg ccagatctag
      241 acgtacgtgg ccaattgggc ccttaattaa ggcctcgttt aaacagtcga cgtcgcccgg
      301 gctgcagcat gcagtgcagc gtgacccggt cgtgcccctc tctagagata atgagcattg
      361 catgtctaag ttataaaaaa ttaccacata ttttttttgt cacacttgtt tgaagtgcag
      421 tttatctatc tttatacata tatttaaact ttactctacg aataatataa tctatagtac
      481 tacaataata tcagtgtttt agagaatcat ataaatgaac agttagacat ggtctaaagg
      541 acaattgagt attttgacaa caggactcta cagttttatc tttttagtgt gcatgtgttc
      601 tccttttttt ttgcaaatag cttcacctat ataatacttc atccatttta ttagtacatc
      661 catttagggt ttagggttaa tggtttttat agactaattt ttttagtaca tctattttat
      721 tctattttag cctctaaatt aagaaaacta aaactctatt ttagtttttt tatttaataa
      781 tttagatata aaatagaata aaataaagtg actaaaaatt aaacaaatac cctttaagaa
      841 attaaaaaaa ctaaggaaac atttttcttg tttcgagtag ataatgccag cctgttaaac
      901 gccgtcgacg agtctaacgg acaccaacca gcgaaccagc agcgtcgcgt cgggccaagc
      961 gaagcagacg gcacggcatc tctgtcgctg cctctggacc cctctcgaga gttccgctcc
     1021 accgttggac ttgctccgct gtcggcatcc agaaattgcg tggcggagcg gcagacgtga
     1081 gccggcacgg caggcggcct cctcctcctc tcacggcacc ggcagctacg ggggattcct
     1141 ttcccaccgc tccttcgctt tcccttcctc gcccgccgta ataaatagac accccctcca
     1201 caccctcttt ccccaacctc gtgttgttcg gagcgcacac acacacaacc agatctcccc
     1261 caaatccacc cgtcggcacc tccgcttcaa ggtacgccgc tcgtcctccc cccccccccc
     1321 tctctacctt ctctagatcg gcgttccggt ccatggttag ggcccggtag ttctacttct
     1381 gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt gctgctagcg ttcgtacacg
     1441 gatgcgacct gtacgtcaga cacgttctga ttgctaactt gccagtgttt ctctttgggg
     1501 aatcctggga tggctctagc cgttccgcag acgggatcga tttcatgatt ttttttgttt
     1561 cgttgcatag ggtttggttt gcccttttcc tttatttcaa tatatgccgt gcacttgttt
     1621 gtcgggtcat cttttcatgc ttttttttgt cttggttgtg atgatgtggt ctggttgggc
     1681 ggtcgttcta gatcggagta gaattctgtt tcaaactacc tggtggattt attaattttg
     1741 gatctgtatg tgtgtgccat acatattcat agttacgaat tgaagatgat ggatggaaat
     1801 atcgatctag gataggtata catgttgatg cgggttttac tgatgcatat acagagatgc
     1861 tttttgttcg cttggttgtg atgatgtggt gtggttgggc ggtcgttcat tcgttctaga
     1921 tcggagtaga atactgtttc aaactacctg gtgtatttat taattttgga actgtatgtg
     1981 tgtgtcatac atcttcatag ttacgagttt aagatggatg gaaatatcga tctaggatag
     2041 gtatacatgt tgatgtgggt tttactgatg catatacatg atggcatatg cagcatctat
     2101 tcatatgctc taaccttgag tacctatcta ttataataaa caagtatgtt ttataattat
     2161 tttgatcttg atatacttgg atgatggcat atgcagcagc tatatgtgga tttttttagc
     2221 cctgccttca tacgctattt atttgcttgg tactgtttct tttgtcgatg ctcaccctgt
     2281 tgtttggtgt tacttctgca tttaaattac cgttcgtata gcatacatta tacgaagtta
     2341 tctacaggaa caggtggtgg cggccctcgg tgcgctcgta ctgctccacg atggtgtagt
     2401 cctcgttgtg ggaggtgatg tccagcttgg cgtccacgta gtagtagccg ggcagctgca
     2461 cgggcttctt ggccatgtag atggacttga actccaccag gtagtggccg ccgtccttca
     2521 gcttcagggc cttgtgggtc tcgcccttca gcacgccgtc gcgggggtac aggcgctcgg
     2581 tggaggcctc ccagcccatg gtcttcttct gcatcacggg gccgtcggag gggaagttca
     2641 cgccgatgaa cttcaccttg tagatgaagc agccgtcctg cagggaggag tcctgggtca
     2701 cggtcgccac gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccctcgggga
     2761 aggacagctt cttgtagtcg gggatgtcgg cggggtgctt cacgtacacc ttggagccgt
     2821 actggaactg gggggacagg atgtcccagg cgaagggcag ggggccgccc ttggtcacct
     2881 tcagcttcac ggtgttgtgg ccctcgtagg ggcggccctc gccctcgccc tcgatctcga
     2941 actcgtggcc gttcacggtg ccctccatgc gcaccttgaa gcgcatgaac tcggtgatga
     3001 cgttctcgga ggaggccatt accgttcgta taatgtatgc tatacgaagt tatactagtg
     3061 gatccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg
     3121 tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat
     3181 gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat
     3241 ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt
     3301 gtcatctatg ttactagatc gggaattcga tatcaagcta tacattcaaa tatgtatccg
     3361 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     3421 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     3481 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     3541 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     3601 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     3661 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     3721 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     3781 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     3841 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     3901 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     3961 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     4021 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     4081 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     4141 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     4201 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     4261 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     4321 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     4381 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4441 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaactgtc agaccaagtt
     4501 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     4561 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     4621 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4681 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4741 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4801 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4861 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4921 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4981 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     5041 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     5101 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa aacgcctggt
     5161 atctttatag tcctgtcggg ttttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5221 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5281 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     5341 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     5401 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     5461 cgttggccga ttcattaatg cagctggcac gacaggtt
//
