LOCUS       Exported                5524 bp ds-DNA     circular SYN 02-JUL-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5524)
  AUTHORS   Birchler Lab
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 29, 2020 from SnapGene 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5524
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(76..95)
                     /label=pSP72R
                     /note="336"
                     /note="color: black; sequence: GAGTCAGTGAGCGAGGAAGC"
     misc_feature    242..1015
                     /label=Only_once
                     /note="color: #ffa552; direction: RIGHT"
     rep_origin      complement(252..842)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(271..290)
                     /label=pBlueMCSR1
                     /note="TM-59.84 Sequence into MCS of pBluescriptIIKS+"
                     /note="color: black; sequence: TTTTTGTGATGCTCGTCAGG"
     misc_feature    1016..2438
                     /label=Contig 2(1&gt;4774)
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    1016..2438
                     /label=Below threshold
                     /note="color: #625fff; direction: RIGHT"
     misc_feature    1016..1610
                     /label=Only_once
                     /note="color: #ffa552; direction: RIGHT"
     CDS             complement(1224..2084)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 1224 .. 2015 / #ccffcc
                        2: 2016 .. 2084 / #ccffcc / signal sequence
                     Cleavage site after base 2015"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1279..1298)
                     /label=pWY56cWalk2
                     /note="TM-60.07 Walk into pWY56c from pWY56cBackbone 
                     primer"
                     /note="color: black; sequence: GGGAGTCAGGCAACTATGGA"
     misc_feature    1611..1792
                     /label=One_strand
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    1793..1940
                     /label=Only_once
                     /note="color: #ffa552; direction: RIGHT"
     primer_bind     complement(1847..1866)
                     /label=pWY56cBackbone F
                     /note="TM-59.98 Walk into pWY56c to connect contigs"
                     /note="color: black; sequence: GCTATGTGGCGCGGTATTAT"
     misc_feature    1941..2289
                     /label=One_strand
                     /note="color: #ff0000; direction: RIGHT"
     promoter        complement(2085..2156)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     2118..2141
                     /label=SacI-Nos
                     /note="Tm-60.2 2374bp with HindII-Ubi"
                     /note="color: black; sequence: 
                     ATTGCATTCGAGCTCAGGGTTATTGTCTCATGAGCGG"
     primer_bind     2173..2204
                     /label=IntExc_rev
                     /note="TM-62.1 NEBuilder primers for LongampTaq"
                     /note="color: black; sequence: 
                     gtacccggggatcctATTCCCGATCTAGTAACATAGATGACACCGCG"
     terminator      2179..2431
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
                     /note="color: #ffffff"
     primer_bind     complement(2188..2206)
                     /label=RandomPCRF1
                     /note="color: black; sequence: CGCGCGGTGTCATCTATGT"
     primer_bind     complement(2260..2283)
                     /label=BarnosSeq3.1
                     /note="TM-59.89"
                     /note="color: black; sequence: TGATTAGAGTCCCGCAATTATACA"
     primer_bind     2267..2289
                     /label=Contig4Fwd2
                     /note="TM-59.98"
                     /note="color: black; sequence: TTGCGGGACTCTAATCATAAAAA"
     primer_bind     2375..2394
                     /label=NOSRev1
                     /note="TM-59.6 In Nos Reverse"
                     /note="color: black; sequence: AAGACCGGCAACAGGATTCA"
     primer_bind     2375..2393
                     /label=Contig4Fwd1
                     /note="TM-60.07"
                     /note="color: black; sequence: AAGACCGGCAACAGGATTC"
     primer_bind     2407..2428
                     /label=pTFPhiR1
                     /note="TM-59.88 Amplify ubi-int-nos for probe (Not full)"
                     /note="color: black; sequence: tttattgccaaatgtttgaacg"
     primer_bind     complement(2410..2429)
                     /label=BarNosSeq2.1
                     /note="TM-59.02"
                     /note="color: black; sequence: TCGTTCAAACATTTGGCAAT"
     misc_feature    2420..2438
                     /label=MCS
                     /note="MCS"
                     /note="color: #0080ff"
     misc_feature    complement(2440..2489)
                     /label=attR
                     /note="color: #ff0000; direction: LEFT"
     CDS             2490..3167
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     misc_feature    3168..3217
                     /label=attL
                     /note="color: #000080; direction: RIGHT"
     misc_feature    3218..5322
                     /label=Contig 2(1&gt;4774)
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    3218..4107
                     /label=Below threshold
                     /note="color: #625fff; direction: RIGHT"
     misc_feature    3218..3224
                     /label=MCS
                     /note="MCS"
                     /note="color: #0080ff"
     promoter        complement(3225..5215)
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(3235..3258)
                     /label=Ubi47-F
                     /note="Bob Designed Primer"
                     /note="color: black; sequence: GATGCTCACCCTGTTGTTTGGTGT"
     misc_feature    3402..3514
                     /label=DNA_JOIN
                     /note="joined contig Contig 2 with Contig 1"
                     /note="color: #0000ff; direction: RIGHT"
     primer_bind     complement(3532..3557)
                     /label=Ubi1660F
                     /note="Bob Designed Primer"
                     /note="color: black; sequence: GGAACTGTATGTGTGTGTCATACATC"
     primer_bind     3538..3560
                     /label=Contig4Fwd3
                     /note="TM-59.01"
                     /note="color: black; sequence: TGACACACACATACAGTTCCAAA"
     primer_bind     3636..3655
                     /label=pWY56cUbiR
                     /note="TM-59.97"
                     /note="color: black; sequence: CCACATCATCACAACCAAGC"
     primer_bind     3636..3655
                     /label=pWY56cWalk1
                     /note="TM-59.97 Walk into pWY56c from end of NOS"
                     /note="color: black; sequence: CCACATCATCACAACCAAGC"
     primer_bind     3636..3655
                     /label=Ubi1592R
                     /note="Bob Designed Primer"
                     /note="color: black; sequence: CCACATCATCACAACCAAGC"
     primer_bind     3769..3788
                     /label=Contig4Fwd3
                     /note="TM-59.01"
                     /note="color: black; sequence: TGACACACACATACAGTTCCAAA"
     misc_feature    3867..3996
                     /label=Only_once
                     /note="color: #ffa552; direction: RIGHT"
     primer_bind     complement(3883..3902)
                     /label=P3F1
                     /note="TM-60 Sequence from back half of Ubi"
                     /note="color: black; sequence: CGGGTCATCTTTTCATGCTT"
     primer_bind     complement(3883..3902)
                     /label=UbiIntF1
                     /note="TM-60.07 Ubi Forward Primer designed for making INT 
                     Fish Probe"
                     /note="color: black; sequence: cgggtcatcttttcatgctt"
     primer_bind     complement(3945..3964)
                     /label=IntegraseProbe3F
                     /note="TM-59.9 Second Half Ubiquitin into Integrase"
                     /note="color: black; sequence: cgttgcatagggtttggttt"
     primer_bind     complement(3945..3964)
                     /label=UbiIntF2
                     /note="TM-59.86 Ubi Forward Primer designed for making INT 
                     Fish Probe"
                     /note="color: black; sequence: cgttgcatagggtttggttt"
     primer_bind     4024..4043
                     /label=Contig6Fwd1
                     /note="TM-60.08"
                     /note="color: black; sequence: TCCCCAAAGAGAAACACTGG"
     primer_bind     4024..4043
                     /label=IntegraseProbe2R
                     /note="TM-60.08 First Half of Ubiquitin"
                     /note="color: black; sequence: tccccaaagagaaacactgg"
     primer_bind     4039..4061
                     /label=Contig6Fwd1 (2)
                     /note="TM-59.84"
                     /note="color: black; sequence: ACTGGCAAGTTAGCAATCAGAAC"
     misc_feature    4108..4109
                     /label=Above threshold
                     /note="color: #00ff00; direction: RIGHT"
     misc_feature    4110..5322
                     /label=Below threshold
                     /note="color: #625fff; direction: RIGHT"
     primer_bind     complement(4146..4165)
                     /label=Ubi1063F
                     /note="Bob Designed Primer"
                     /note="color: black; sequence: GGGCCCGGTAGTTCTACTTC"
     misc_feature    4224..4451
                     /label=Only_once
                     /note="color: #ffa552; direction: RIGHT"
     primer_bind     4259..4278
                     /label=IntegraseProbe2R2
                     /note="Tm-60.1 First Half of Ubiquitin"
                     /note="color: black; sequence: gatttgggggagatctggtt"
     misc_feature    4452..5322
                     /label=One_strand
                     /note="color: #ff0000; direction: RIGHT"
     primer_bind     complement(4467..4486)
                     /label=Contig2Rvs1
                     /note="TM-61.57"
                     /note="color: black; sequence: CTGTCGGCATCCAGAAATTG"
     primer_bind     complement(4594..4615)
                     /label=Contig2Rvs2
                     /note="TM-60.96"
                     /note="color: black; sequence: GAGTCTAACGGACACCAACCAG"
     primer_bind     4917..4940
                     /label=UbiPRev1
                     /note="color: black; sequence: AAAAAGGAGAACACATGCACACTA"
     primer_bind     complement(4917..4938)
                     /label=pTFPhiF1
                     /note="TM-59.15 Amplify ubi-int-nos for probe (Not full)"
                     /note="color: black; sequence: gtgtgcatgtgttctccttttt"
     primer_bind     complement(4917..4938)
                     /label=pTFPhiF2
                     /note="TM-59.15 Amplify ubi-int-nos for probe (Not full)"
                     /note="color: black; sequence: gtgtgcatgtgttctccttttt"
     primer_bind     complement(4951..4970)
                     /label=Ubi2-F
                     /note="color: black; sequence: TGACAACAGGACTCTACAGT"
     primer_bind     complement(5105..5126)
                     /label=UbiF2
                     /note="TM-58.48 Primer of UBI before primer begins on GZUS"
                     /note="color: black; sequence: GTCACACTTGTTTGAAGTGCAG"
     primer_bind     complement(5107..5128)
                     /label=Ubi1F1
                     /note="color: black; sequence: TTGTCACACTTGTTTGAAGTGC"
     primer_bind     complement(5107..5128)
                     /label=UbiF1
                     /note="TM-58.88 Primer of UBI before primer begins on GZUS"
                     /note="color: black; sequence: TTGTCACACTTGTTTGAAGTGC"
     primer_bind     complement(5153..5170)
                     /label=IntegraseProbe2F
                     /note="Tm-58.95 First Half of Ubiquitin"
                     /note="color: black; sequence: gcgcattgcatgtctaagtt"
     primer_bind     complement(5165..5185)
                     /label=Ubi1F
                     /note="color: black; sequence: CTCTAGAGATAATGAGCATTG"
     primer_bind     complement(5184..5203)
                     /label=IntExc_fwd
                     /note="TM-62.1 NEBuilder primers for LongampTaq"
                     /note="color: black; sequence: 
                     caggcatgcaagctcaTGACCCGGTCGTGCCCCTCT"
     primer_bind     complement(5199..5216)
                     /label=HIIInoBUbi56GFP-F
                     /note="Amplify Ubi-EGFP-Nos from p56EGFP to insert into 
                     pZY101"
                     /note="color: black; sequence: 
                     GCTTAAAGCTTATTATGCAGTGCAGCGTGACC"
     misc_feature    5201..5322
                     /label=pMECA
                     /note="pMECA"
                     /note="color: #0080ff"
     primer_bind     complement(5206..5218)
                     /label=UbiFullNoHINDIII
                     /note="TM-61.15 Full Ubi No HINDIII"
                     /note="color: black; sequence: GCTTGCATGCCTGCAGTGCAG"
     primer_bind     complement(5227..5249)
                     /label=HindIII-Ubi
                     /note="Tm-60.7 2374bp with SacI-Nos Insert Ubi Nos into 
                     pTF"
                     /note="color: black; sequence: 
                     GATGATTAAAGCTTaCGTTTAAACAGTCGACGTCGCCC"
     primer_bind     5227..5245
                     /label=pMECAControl64
                     /note="TM-58 Screen for pMECA insert into pNG2 65bp Product
                     with Screen1, only for use with pWY82-Full"
                     /note="color: black; sequence: gggcgacgtcgactgttta"
     primer_bind     complement(5270..5291)
                     /label=pMECAScreen1
                     /note="TM-60 Screen for pMECA insert into pNG2 100bp to 
                     FSE1"
                     /note="color: black; sequence: gatctagacgtacgtggccaat"
     primer_bind     complement(5280..5298)
                     /label=pMECARvsBsaAI
                     /note="TM-60.13 Primer Rvs in pMECA BsaAI-BssHii"
                     /note="color: black; sequence: cgcgccagatctagacgta"
     primer_bind     complement(5280..5298)
                     /label=pMECAScreen2
                     /note="TM-59.1 Screen for pMECA insert into pNG2 107bp to 
                     FSE1"
                     /note="color: black; sequence: cgcgccagatctagacgta"
     misc_feature    join(5323..5524,1..241)
                     /label=One_strand
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    5323
                     /label=Below threshold
                     /note="This forward directional feature has 3 segments:
                        1: 5323 .. 1013 / #a6acb3
                        2: 1014 .. 1015 / #------
                        3: 1016 .. 5323 / #a6acb3"
     misc_feature    5323
                     /label=Contig 2(1&gt;4774)
                     /note="This forward directional feature has 3 segments:
                        1: 5323 .. 1013 / #a6acb3
                        2: 1014 .. 1015 / #------
                        3: 1016 .. 5323 / #a6acb3"
     promoter        complement(5335..5353)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(5374..5395)
                     /label=m13R-23mer
                     /note="color: black; sequence: TCACACAGGAAACAGCTATGAC"
     primer_bind     complement(5374..5390)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(5374..5390)
                     /label=m13R-17mer
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     protein_bind    5398..5414
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     complement(5402..5421)
                     /label=pGEMScreenR
                     /note="TM-60.07 Screen for pGEMT or Easy Inserts 163 to 
                     Insert (Easy)"
                     /note="color: black; sequence: TGTGGAATTGTGAGCGGATA"
     primer_bind     complement(5402..5421)
                     /label=topo2.1ScreenF
                     /note="TM-60.07 Sequence across topo insert 232 without 
                     insert"
                     /note="color: black; sequence: TGTGGAATTGTGAGCGGATA"
     primer_bind     complement(5408..5430)
                     /label=ptf101TeloR
                     /note="TM-60.33 Sequence into Telo"
                     /note="color: black; sequence: CGTATGTTGTGTGGAATTGTGAG"
     primer_bind     complement(5410..5432)
                     /label=ptf101TeloR2
                     /note="TM- 60.33 Sequence into Telo of ptf101.1 and 
                     derivatives from RB into EcoRI site"
                     /note="color: black; sequence: CTCGTATGTTGTGTGGAATTGTG"
     promoter        complement(5422..5452)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 5422 .. 5428 / #ffffff / -10
                        2: 5429 .. 5446 / #ffffff
                        3: 5447 .. 5452 / #ffffff / -35"
     protein_bind    5467..5488
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
ORIGIN
        1 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
       61 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      121 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      181 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      241 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      301 agtcagaggt ggcgaaaacc cgacaggact ataaagatac caggcgtttt ccccctggaa
      361 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      421 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      481 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      541 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      601 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      661 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      721 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      781 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      841 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
      901 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
      961 aatgaagttt taaatcaatc taaagtatat atgagtaaaa cttggtctga cagttgcaag
     1021 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1081 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     1141 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     1201 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     1261 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     1321 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     1381 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     1441 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     1501 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     1561 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     1621 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     1681 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     1741 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     1801 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     1861 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     1921 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     1981 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     2041 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     2101 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtata
     2161 gcttgatatc gaattcccga tctagtaaca tagatgacac cgcgcgcgat aatttatcct
     2221 agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa
     2281 tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa
     2341 cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta
     2401 agaaacttta ttgccaaatg tttgaacgat cggggatccg tggagtacgc gcccggggag
     2461 cccaaaggtt accccagttg gggcactaca tggcctcctc cgagaacgtc atcaccgagt
     2521 tcatgcgctt caaggtgcgc atggagggca ccgtgaacgg ccacgagttc gagatcgagg
     2581 gcgagggcga gggccgcccc tacgagggcc acaacaccgt gaagctgaag gtgaccaagg
     2641 gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttccagtac ggctccaagg
     2701 tgtacgtgaa gcaccccgcc gacatccccg actacaagaa gctgtccttc cccgagggct
     2761 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggcgaccgtg acccaggact
     2821 cctccctgca ggacggctgc ttcatctaca aggtgaagtt catcggcgtg aacttcccct
     2881 ccgacggccc cgtgatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt
     2941 acccccgcga cggcgtgctg aagggcgaga cccacaaggc cctgaagctg aaggacggcg
     3001 gccactacct ggtggagttc aagtccatct acatggccaa gaagcccgtg cagctgcccg
     3061 gctactacta cgtggacgcc aagctggaca tcacctccca caacgaggac tacaccatcg
     3121 tggagcagta cgagcgcacc gagggccgcc accacctgtt cctgtagcgg tgcgggtgcc
     3181 agggcgtgcc cttgagttct ctcagttggg ggcgtagatt taaatgcaga agtaacacca
     3241 aacaacaggg tgagcatcga caaaagaaac agtaccaagc aaataaatag cgtatgaagg
     3301 cagggctaaa aaaatccaca tatagctgct gcatatgcca tcatccaagt atatcaagat
     3361 caaaataatt ataaaacata cttgtttatt ataatagata ggtactcaag gttagagcat
     3421 atgaatagat gctgcatatg ccatcatgta tatgcatcag taaaacccac atcaacatgt
     3481 atacctatcc tagatcgata tttccatcca tcttaaactc gtaactatga agatgtatga
     3541 cacacacata cagttccaaa attaataaat acaccaggta gtttgaaaca gtattctact
     3601 ccgatctaga acgaatgaac gaccgcccaa ccacaccaca tcatcacaac caagcgaaca
     3661 aaaagcatct ctgtatatgc atcagtaaaa cccgcatcaa catgtatacc tatcctagat
     3721 cgatatttcc atccatcatc ttcaattcgt aactatgaat atgtatggca cacacataca
     3781 gatccaaaat taataaatcc accaggtagt ttgaaacaga attctactcc gatctagaac
     3841 gaccgcccaa ccagaccaca tcatcacaac caagacaaaa aaaagcatga aaagatgacc
     3901 cgacaaacaa gtgcacggca tatattgaaa taaaggaaaa gggcaaacca aaccctatgc
     3961 aacgaaacaa aaaaaatcat gaaatcgatc ccgtctgcgg aacggctaga gccatcccag
     4021 gattccccaa agagaaacac tggcaagtta gcaatcagaa cgtgtctgac gtacaggtcg
     4081 catccgtgta cgaacgctag cagcacggat ctaacacaaa cacggatcta acacaaacat
     4141 gaacagaagt agaactaccg ggccctaacc atggaccgga acgccgatct agagaaggta
     4201 gagagggggg gggggggagg acgagcggcg taccttgaag cggaggtgcc gacgggtgga
     4261 tttgggggag atctggttgt gtgtgtgtgc gctccgaaca acacgaggtt ggggaaagag
     4321 ggtgtggagg gggtgtctat ttattacggc gggcgaggaa gggaaagcga aggagcggtg
     4381 ggaaaggaat cccccgtagc tgccggtgcc gtgagaggag gaggaggccg cctgccgtgc
     4441 cggctcacgt ctgccgctcc gccacgcaat ttctggatgc cgacagcgga gcaagtccaa
     4501 cggtggagcg gaactctcga gaggggtcca gaggcagcga cagagatgcc gtgccgtctg
     4561 cttcgcttgg cccgacgcga cgctgctggt tcgctggttg gtgtccgtta gactcgtcga
     4621 cggcgtttaa caggctggca ttatctactc gaaacaagaa aaatgtttcc ttagtttttt
     4681 taatttctta aagggtattt gtttaatttt tagtcacttt attttattct attttatatc
     4741 taaattatta aataaaaaaa ctaaaataga gttttagttt tcttaattta gaggctaaaa
     4801 tagaataaaa tagatgtact aaaaaaatta gtctataaaa accattaacc ctaaacccta
     4861 aatggatgta ctaataaaat ggatgaagta ttatataggt gaagctattt gcaaaaaaaa
     4921 aggagaacac atgcacacta aaaagataaa actgtagagt cctgttgtca aaatactcaa
     4981 ttgtccttta gaccatgtct aactgttcat ttatatgatt ctctaaaaca ctgatattat
     5041 tgtagtacta tagattatat tattcgtaga gtaaagttta aatatatgta taaagataga
     5101 taaactgcac ttcaaacaag tgtgacaaaa aaaatatgtg gtaatttttt ataacttaga
     5161 catgcaatgc tcattatctc tagagagggg cacgaccggg tcacgctgca ctgcatgctg
     5221 cagcccgggc gacgtcgact gtttaaacga ggccttaatt aagggcccaa ttggccacgt
     5281 acgtctagat ctggcgcgcc tcgaggatcg atccatggta cccagctttt gttcccttta
     5341 gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     5401 ttatccgctc acaattccac acaacatacg agccgggagc ataaagtgta aagcctgggg
     5461 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     5521 ggga
//
