LOCUS       Exported                5498 bp ds-DNA     circular SYN 09-JAN-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Birchler Lab
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 29, 2020 from SnapGene 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(76..95)
                     /label=pSP72R
                     /note="336"
                     /note="color: black; sequence: GAGTCAGTGAGCGAGGAAGC"
     misc_feature    242..1015
                     /label=Only_once
                     /note="color: #ffa552; direction: RIGHT"
     rep_origin      complement(252..842)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(271..290)
                     /label=pBlueMCSR1
                     /note="TM-59.84 Sequence into MCS of pBluescriptIIKS+"
                     /note="color: black; sequence: TTTTTGTGATGCTCGTCAGG"
     misc_feature    1016..2440
                     /label=Contig 2(1&gt;4774)
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    1016..2440
                     /label=Below threshold
                     /note="color: #625fff; direction: RIGHT"
     misc_feature    1016..1610
                     /label=Only_once
                     /note="color: #ffa552; direction: RIGHT"
     CDS             complement(1224..2084)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 1224 .. 2015 / #ccffcc
                        2: 2016 .. 2084 / #ccffcc / signal sequence
                     Cleavage site after base 2015"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1279..1298)
                     /label=pWY56cWalk2
                     /note="TM-60.07 Walk into pWY56c from pWY56cBackbone 
                     primer"
                     /note="color: black; sequence: GGGAGTCAGGCAACTATGGA"
     misc_feature    1611..1792
                     /label=One_strand
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    1793..1940
                     /label=Only_once
                     /note="color: #ffa552; direction: RIGHT"
     primer_bind     complement(1847..1866)
                     /label=pWY56cBackbone F
                     /note="TM-59.98 Walk into pWY56c to connect contigs"
                     /note="color: black; sequence: GCTATGTGGCGCGGTATTAT"
     misc_feature    1941..2289
                     /label=One_strand
                     /note="color: #ff0000; direction: RIGHT"
     promoter        complement(2085..2156)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     2118..2141
                     /label=SacI-Nos
                     /note="Tm-60.2 2374bp with HindII-Ubi"
                     /note="color: black; sequence: 
                     ATTGCATTCGAGCTCAGGGTTATTGTCTCATGAGCGG"
     primer_bind     2173..2204
                     /label=IntExc_rev
                     /note="TM-62.1 NEBuilder primers for LongampTaq"
                     /note="color: black; sequence: 
                     gtacccggggatcctATTCCCGATCTAGTAACATAGATGACACCGCG"
     terminator      2179..2431
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
                     /note="color: #ffffff"
     primer_bind     complement(2188..2206)
                     /label=RandomPCRF1
                     /note="color: black; sequence: CGCGCGGTGTCATCTATGT"
     primer_bind     complement(2260..2283)
                     /label=BarnosSeq3.1
                     /note="TM-59.89"
                     /note="color: black; sequence: TGATTAGAGTCCCGCAATTATACA"
     primer_bind     2267..2289
                     /label=Contig4Fwd2
                     /note="TM-59.98"
                     /note="color: black; sequence: TTGCGGGACTCTAATCATAAAAA"
     primer_bind     2375..2394
                     /label=NOSRev1
                     /note="TM-59.6 In Nos Reverse"
                     /note="color: black; sequence: AAGACCGGCAACAGGATTCA"
     primer_bind     2375..2393
                     /label=Contig4Fwd1
                     /note="TM-60.07"
                     /note="color: black; sequence: AAGACCGGCAACAGGATTC"
     primer_bind     2407..2428
                     /label=pTFPhiR1
                     /note="TM-59.88 Amplify ubi-int-nos for probe (Not full)"
                     /note="color: black; sequence: tttattgccaaatgtttgaacg"
     primer_bind     complement(2410..2429)
                     /label=BarNosSeq2.1
                     /note="TM-59.02"
                     /note="color: black; sequence: TCGTTCAAACATTTGGCAAT"
     misc_feature    2420..2440
                     /label=MCS
                     /note="MCS"
                     /note="color: #0080ff"
     protein_bind    2446..2479
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
                     /note="color: #31849b; direction: RIGHT"
     CDS             2480..3157
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     protein_bind    complement(3158..3191)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
                     /note="color: #31849b; direction: LEFT"
     misc_feature    3196..5296
                     /label=Contig 2(1&gt;4774)
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    3196..4081
                     /label=Below threshold
                     /note="color: #625fff; direction: RIGHT"
     misc_feature    3196..3198
                     /label=MCS
                     /note="MCS"
                     /note="color: #0080ff"
     promoter        complement(3199..5189)
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(3209..3232)
                     /label=Ubi47-F
                     /note="Bob Designed Primer"
                     /note="color: black; sequence: GATGCTCACCCTGTTGTTTGGTGT"
     misc_feature    3376..3488
                     /label=DNA_JOIN
                     /note="joined contig Contig 2 with Contig 1"
                     /note="color: #0000ff; direction: RIGHT"
     primer_bind     complement(3506..3531)
                     /label=Ubi1660F
                     /note="Bob Designed Primer"
                     /note="color: black; sequence: GGAACTGTATGTGTGTGTCATACATC"
     primer_bind     3512..3534
                     /label=Contig4Fwd3
                     /note="TM-59.01"
                     /note="color: black; sequence: TGACACACACATACAGTTCCAAA"
     primer_bind     3610..3629
                     /label=pWY56cUbiR
                     /note="TM-59.97"
                     /note="color: black; sequence: CCACATCATCACAACCAAGC"
     primer_bind     3610..3629
                     /label=pWY56cWalk1
                     /note="TM-59.97 Walk into pWY56c from end of NOS"
                     /note="color: black; sequence: CCACATCATCACAACCAAGC"
     primer_bind     3610..3629
                     /label=Ubi1592R
                     /note="Bob Designed Primer"
                     /note="color: black; sequence: CCACATCATCACAACCAAGC"
     primer_bind     3743..3762
                     /label=Contig4Fwd3
                     /note="TM-59.01"
                     /note="color: black; sequence: TGACACACACATACAGTTCCAAA"
     misc_feature    3841..3970
                     /label=Only_once
                     /note="color: #ffa552; direction: RIGHT"
     primer_bind     complement(3857..3876)
                     /label=P3F1
                     /note="TM-60 Sequence from back half of Ubi"
                     /note="color: black; sequence: CGGGTCATCTTTTCATGCTT"
     primer_bind     complement(3857..3876)
                     /label=UbiIntF1
                     /note="TM-60.07 Ubi Forward Primer designed for making INT 
                     Fish Probe"
                     /note="color: black; sequence: cgggtcatcttttcatgctt"
     primer_bind     complement(3919..3938)
                     /label=IntegraseProbe3F
                     /note="TM-59.9 Second Half Ubiquitin into Integrase"
                     /note="color: black; sequence: cgttgcatagggtttggttt"
     primer_bind     complement(3919..3938)
                     /label=UbiIntF2
                     /note="TM-59.86 Ubi Forward Primer designed for making INT 
                     Fish Probe"
                     /note="color: black; sequence: cgttgcatagggtttggttt"
     primer_bind     3998..4017
                     /label=Contig6Fwd1
                     /note="TM-60.08"
                     /note="color: black; sequence: TCCCCAAAGAGAAACACTGG"
     primer_bind     3998..4017
                     /label=IntegraseProbe2R
                     /note="TM-60.08 First Half of Ubiquitin"
                     /note="color: black; sequence: tccccaaagagaaacactgg"
     primer_bind     4013..4035
                     /label=Contig6Fwd1 (2)
                     /note="TM-59.84"
                     /note="color: black; sequence: ACTGGCAAGTTAGCAATCAGAAC"
     misc_feature    4082..4083
                     /label=Above threshold
                     /note="color: #00ff00; direction: RIGHT"
     misc_feature    4084..5296
                     /label=Below threshold
                     /note="color: #625fff; direction: RIGHT"
     primer_bind     complement(4120..4139)
                     /label=Ubi1063F
                     /note="Bob Designed Primer"
                     /note="color: black; sequence: GGGCCCGGTAGTTCTACTTC"
     misc_feature    4198..4425
                     /label=Only_once
                     /note="color: #ffa552; direction: RIGHT"
     primer_bind     4233..4252
                     /label=IntegraseProbe2R2
                     /note="Tm-60.1 First Half of Ubiquitin"
                     /note="color: black; sequence: gatttgggggagatctggtt"
     misc_feature    4426..5296
                     /label=One_strand
                     /note="color: #ff0000; direction: RIGHT"
     primer_bind     complement(4441..4460)
                     /label=Contig2Rvs1
                     /note="TM-61.57"
                     /note="color: black; sequence: CTGTCGGCATCCAGAAATTG"
     primer_bind     complement(4568..4589)
                     /label=Contig2Rvs2
                     /note="TM-60.96"
                     /note="color: black; sequence: GAGTCTAACGGACACCAACCAG"
     primer_bind     4891..4914
                     /label=UbiPRev1
                     /note="color: black; sequence: AAAAAGGAGAACACATGCACACTA"
     primer_bind     complement(4891..4912)
                     /label=pTFPhiF1
                     /note="TM-59.15 Amplify ubi-int-nos for probe (Not full)"
                     /note="color: black; sequence: gtgtgcatgtgttctccttttt"
     primer_bind     complement(4891..4912)
                     /label=pTFPhiF2
                     /note="TM-59.15 Amplify ubi-int-nos for probe (Not full)"
                     /note="color: black; sequence: gtgtgcatgtgttctccttttt"
     primer_bind     complement(4925..4944)
                     /label=Ubi2-F
                     /note="color: black; sequence: TGACAACAGGACTCTACAGT"
     primer_bind     complement(5079..5100)
                     /label=UbiF2
                     /note="TM-58.48 Primer of UBI before primer begins on GZUS"
                     /note="color: black; sequence: GTCACACTTGTTTGAAGTGCAG"
     primer_bind     complement(5081..5102)
                     /label=Ubi1F1
                     /note="color: black; sequence: TTGTCACACTTGTTTGAAGTGC"
     primer_bind     complement(5081..5102)
                     /label=UbiF1
                     /note="TM-58.88 Primer of UBI before primer begins on GZUS"
                     /note="color: black; sequence: TTGTCACACTTGTTTGAAGTGC"
     primer_bind     complement(5127..5144)
                     /label=IntegraseProbe2F
                     /note="Tm-58.95 First Half of Ubiquitin"
                     /note="color: black; sequence: gcgcattgcatgtctaagtt"
     primer_bind     complement(5139..5159)
                     /label=Ubi1F
                     /note="color: black; sequence: CTCTAGAGATAATGAGCATTG"
     primer_bind     complement(5158..5177)
                     /label=IntExc_fwd
                     /note="TM-62.1 NEBuilder primers for LongampTaq"
                     /note="color: black; sequence: 
                     caggcatgcaagctcaTGACCCGGTCGTGCCCCTCT"
     primer_bind     complement(5173..5190)
                     /label=HIIInoBUbi56GFP-F
                     /note="Amplify Ubi-EGFP-Nos from p56EGFP to insert into 
                     pZY101"
                     /note="color: black; sequence: 
                     GCTTAAAGCTTATTATGCAGTGCAGCGTGACC"
     misc_feature    5175..5296
                     /label=pMECA
                     /note="pMECA"
                     /note="color: #0080ff"
     primer_bind     complement(5180..5192)
                     /label=UbiFullNoHINDIII
                     /note="TM-61.15 Full Ubi No HINDIII"
                     /note="color: black; sequence: GCTTGCATGCCTGCAGTGCAG"
     primer_bind     complement(5201..5223)
                     /label=HindIII-Ubi
                     /note="Tm-60.7 2374bp with SacI-Nos Insert Ubi Nos into 
                     pTF"
                     /note="color: black; sequence: 
                     GATGATTAAAGCTTaCGTTTAAACAGTCGACGTCGCCC"
     primer_bind     5201..5219
                     /label=pMECAControl64
                     /note="TM-58 Screen for pMECA insert into pNG2 65bp Product
                     with Screen1, only for use with pWY82-Full"
                     /note="color: black; sequence: gggcgacgtcgactgttta"
     primer_bind     complement(5244..5265)
                     /label=pMECAScreen1
                     /note="TM-60 Screen for pMECA insert into pNG2 100bp to 
                     FSE1"
                     /note="color: black; sequence: gatctagacgtacgtggccaat"
     primer_bind     complement(5254..5272)
                     /label=pMECARvsBsaAI
                     /note="TM-60.13 Primer Rvs in pMECA BsaAI-BssHii"
                     /note="color: black; sequence: cgcgccagatctagacgta"
     primer_bind     complement(5254..5272)
                     /label=pMECAScreen2
                     /note="TM-59.1 Screen for pMECA insert into pNG2 107bp to 
                     FSE1"
                     /note="color: black; sequence: cgcgccagatctagacgta"
     misc_feature    join(5297..5498,1..241)
                     /label=One_strand
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    5297
                     /label=Below threshold
                     /note="This forward directional feature has 3 segments:
                        1: 5297 .. 1013 / #a6acb3
                        2: 1014 .. 1015 / #------
                        3: 1016 .. 5297 / #a6acb3"
     misc_feature    5297
                     /label=Contig 2(1&gt;4774)
                     /note="This forward directional feature has 3 segments:
                        1: 5297 .. 1013 / #a6acb3
                        2: 1014 .. 1015 / #------
                        3: 1016 .. 5297 / #a6acb3"
     promoter        complement(5309..5327)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(5348..5369)
                     /label=m13R-23mer
                     /note="color: black; sequence: TCACACAGGAAACAGCTATGAC"
     primer_bind     complement(5348..5364)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(5348..5364)
                     /label=m13R-17mer
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     protein_bind    5372..5388
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     complement(5376..5395)
                     /label=pGEMScreenR
                     /note="TM-60.07 Screen for pGEMT or Easy Inserts 163 to 
                     Insert (Easy)"
                     /note="color: black; sequence: TGTGGAATTGTGAGCGGATA"
     primer_bind     complement(5376..5395)
                     /label=topo2.1ScreenF
                     /note="TM-60.07 Sequence across topo insert 232 without 
                     insert"
                     /note="color: black; sequence: TGTGGAATTGTGAGCGGATA"
     primer_bind     complement(5382..5404)
                     /label=ptf101TeloR
                     /note="TM-60.33 Sequence into Telo"
                     /note="color: black; sequence: CGTATGTTGTGTGGAATTGTGAG"
     primer_bind     complement(5384..5406)
                     /label=ptf101TeloR2
                     /note="TM- 60.33 Sequence into Telo of ptf101.1 and 
                     derivatives from RB into EcoRI site"
                     /note="color: black; sequence: CTCGTATGTTGTGTGGAATTGTG"
     promoter        complement(5396..5426)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 5396 .. 5402 / #ffffff / -10
                        2: 5403 .. 5420 / #ffffff
                        3: 5421 .. 5426 / #ffffff / -35"
     protein_bind    5441..5462
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
ORIGIN
        1 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
       61 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      121 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      181 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      241 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      301 agtcagaggt ggcgaaaacc cgacaggact ataaagatac caggcgtttt ccccctggaa
      361 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      421 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      481 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      541 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      601 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      661 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      721 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      781 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      841 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
      901 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
      961 aatgaagttt taaatcaatc taaagtatat atgagtaaaa cttggtctga cagttgcaag
     1021 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1081 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     1141 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     1201 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     1261 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     1321 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     1381 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     1441 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     1501 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     1561 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     1621 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     1681 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     1741 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     1801 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     1861 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     1921 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     1981 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     2041 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     2101 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtata
     2161 gcttgatatc gaattcccga tctagtaaca tagatgacac cgcgcgcgat aatttatcct
     2221 agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa
     2281 tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa
     2341 cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta
     2401 agaaacttta ttgccaaatg tttgaacgat cggggatcca ctagtgaagt tcctattctc
     2461 tagaaagtat aggaacttca tggcctcctc cgagaacgtc atcaccgagt tcatgcgctt
     2521 caaggtgcgc atggagggca ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     2581 gggccgcccc tacgagggcc acaacaccgt gaagctgaag gtgaccaagg gcggccccct
     2641 gcccttcgcc tgggacatcc tgtcccccca gttccagtac ggctccaagg tgtacgtgaa
     2701 gcaccccgcc gacatccccg actacaagaa gctgtccttc cccgagggct tcaagtggga
     2761 gcgcgtgatg aacttcgagg acggcggcgt ggcgaccgtg acccaggact cctccctgca
     2821 ggacggctgc ttcatctaca aggtgaagtt catcggcgtg aacttcccct ccgacggccc
     2881 cgtgatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga
     2941 cggcgtgctg aagggcgaga cccacaaggc cctgaagctg aaggacggcg gccactacct
     3001 ggtggagttc aagtccatct acatggccaa gaagcccgtg cagctgcccg gctactacta
     3061 cgtggacgcc aagctggaca tcacctccca caacgaggac tacaccatcg tggagcagta
     3121 cgagcgcacc gagggccgcc accacctgtt cctgtaggaa gttcctatac tttctagaga
     3181 ataggaactt catttaaatg cagaagtaac accaaacaac agggtgagca tcgacaaaag
     3241 aaacagtacc aagcaaataa atagcgtatg aaggcagggc taaaaaaatc cacatatagc
     3301 tgctgcatat gccatcatcc aagtatatca agatcaaaat aattataaaa catacttgtt
     3361 tattataata gataggtact caaggttaga gcatatgaat agatgctgca tatgccatca
     3421 tgtatatgca tcagtaaaac ccacatcaac atgtatacct atcctagatc gatatttcca
     3481 tccatcttaa actcgtaact atgaagatgt atgacacaca catacagttc caaaattaat
     3541 aaatacacca ggtagtttga aacagtattc tactccgatc tagaacgaat gaacgaccgc
     3601 ccaaccacac cacatcatca caaccaagcg aacaaaaagc atctctgtat atgcatcagt
     3661 aaaacccgca tcaacatgta tacctatcct agatcgatat ttccatccat catcttcaat
     3721 tcgtaactat gaatatgtat ggcacacaca tacagatcca aaattaataa atccaccagg
     3781 tagtttgaaa cagaattcta ctccgatcta gaacgaccgc ccaaccagac cacatcatca
     3841 caaccaagac aaaaaaaagc atgaaaagat gacccgacaa acaagtgcac ggcatatatt
     3901 gaaataaagg aaaagggcaa accaaaccct atgcaacgaa acaaaaaaaa tcatgaaatc
     3961 gatcccgtct gcggaacggc tagagccatc ccaggattcc ccaaagagaa acactggcaa
     4021 gttagcaatc agaacgtgtc tgacgtacag gtcgcatccg tgtacgaacg ctagcagcac
     4081 ggatctaaca caaacacgga tctaacacaa acatgaacag aagtagaact accgggccct
     4141 aaccatggac cggaacgccg atctagagaa ggtagagagg gggggggggg gaggacgagc
     4201 ggcgtacctt gaagcggagg tgccgacggg tggatttggg ggagatctgg ttgtgtgtgt
     4261 gtgcgctccg aacaacacga ggttggggaa agagggtgtg gagggggtgt ctatttatta
     4321 cggcgggcga ggaagggaaa gcgaaggagc ggtgggaaag gaatcccccg tagctgccgg
     4381 tgccgtgaga ggaggaggag gccgcctgcc gtgccggctc acgtctgccg ctccgccacg
     4441 caatttctgg atgccgacag cggagcaagt ccaacggtgg agcggaactc tcgagagggg
     4501 tccagaggca gcgacagaga tgccgtgccg tctgcttcgc ttggcccgac gcgacgctgc
     4561 tggttcgctg gttggtgtcc gttagactcg tcgacggcgt ttaacaggct ggcattatct
     4621 actcgaaaca agaaaaatgt ttccttagtt tttttaattt cttaaagggt atttgtttaa
     4681 tttttagtca ctttatttta ttctatttta tatctaaatt attaaataaa aaaactaaaa
     4741 tagagtttta gttttcttaa tttagaggct aaaatagaat aaaatagatg tactaaaaaa
     4801 attagtctat aaaaaccatt aaccctaaac cctaaatgga tgtactaata aaatggatga
     4861 agtattatat aggtgaagct atttgcaaaa aaaaaggaga acacatgcac actaaaaaga
     4921 taaaactgta gagtcctgtt gtcaaaatac tcaattgtcc tttagaccat gtctaactgt
     4981 tcatttatat gattctctaa aacactgata ttattgtagt actatagatt atattattcg
     5041 tagagtaaag tttaaatata tgtataaaga tagataaact gcacttcaaa caagtgtgac
     5101 aaaaaaaata tgtggtaatt ttttataact tagacatgca atgctcatta tctctagaga
     5161 ggggcacgac cgggtcacgc tgcactgcat gctgcagccc gggcgacgtc gactgtttaa
     5221 acgaggcctt aattaagggc ccaattggcc acgtacgtct agatctggcg cgcctcgagg
     5281 atcgatccat ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
     5341 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     5401 tacgagccgg gagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     5461 taattgcgtt gcgctcactg cccgctttcc agtcggga
//
