LOCUS       Exported                5792 bp ds-DNA     circular SYN 02-JUL-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Birchler Lab
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 29, 2020 from SnapGene 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    263..1685
                     /label=Contig 2(1&gt;4774)
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    263..1685
                     /label=Below threshold
                     /note="color: #625fff; direction: RIGHT"
     misc_feature    263..857
                     /label=Only_once
                     /note="color: #ffa552; direction: RIGHT"
     CDS             complement(471..1331)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  471 .. 1262 / #ccffcc
                        2: 1263 .. 1331 / #ccffcc / signal sequence
                     Cleavage site after base 1262"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(526..545)
                     /label=pWY56cWalk2
                     /note="TM-60.07 Walk into pWY56c from pWY56cBackbone 
                     primer"
                     /note="color: black; sequence: GGGAGTCAGGCAACTATGGA"
     misc_feature    858..1039
                     /label=One_strand
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    1040..1187
                     /label=Only_once
                     /note="color: #ffa552; direction: RIGHT"
     primer_bind     1094..1113
                     /label=SacI-AntiGusR
                     /note="color: black; sequence: 
                     TGGACTCAGAGCTCATAATACCGCGCCACATAGC"
     primer_bind     complement(1094..1113)
                     /label=pWY56cBackbone F
                     /note="TM-59.98 Walk into pWY56c to connect contigs"
                     /note="color: black; sequence: GCTATGTGGCGCGGTATTAT"
     misc_feature    1188..1536
                     /label=One_strand
                     /note="color: #ff0000; direction: RIGHT"
     promoter        complement(1332..1403)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     1420..1451
                     /label=IntExc_rev
                     /note="TM-62.1 NEBuilder primers for LongampTaq"
                     /note="color: black; sequence: 
                     gtacccggggatcctATTCCCGATCTAGTAACATAGATGACACCGCG"
     terminator      1426..1678
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
                     /note="color: #ffffff"
     primer_bind     complement(1435..1453)
                     /label=RandomPCRF1
                     /note="color: black; sequence: CGCGCGGTGTCATCTATGT"
     misc_feature    1471..1491
                     /label=Nos238-R
                     /note="Nos238-R"
                     /note="color: #50ff50; direction: RIGHT"
     primer_bind     complement(1507..1530)
                     /label=BarnosSeq3.1
                     /note="TM-59.89"
                     /note="color: black; sequence: TGATTAGAGTCCCGCAATTATACA"
     primer_bind     1514..1536
                     /label=Contig4Fwd2
                     /note="TM-59.98"
                     /note="color: black; sequence: TTGCGGGACTCTAATCATAAAAA"
     primer_bind     1622..1641
                     /label=NOSRev1
                     /note="TM-59.6 In Nos Reverse"
                     /note="color: black; sequence: AAGACCGGCAACAGGATTCA"
     primer_bind     1622..1640
                     /label=Contig4Fwd1
                     /note="TM-60.07"
                     /note="color: black; sequence: AAGACCGGCAACAGGATTC"
     primer_bind     1654..1675
                     /label=pTFPhiR1
                     /note="TM-59.88 Amplify ubi-int-nos for probe (Not full)"
                     /note="color: black; sequence: tttattgccaaatgtttgaacg"
     primer_bind     complement(1657..1676)
                     /label=BarNosSeq2.1
                     /note="TM-59.02"
                     /note="color: black; sequence: TCGTTCAAACATTTGGCAAT"
     misc_feature    1667..1685
                     /label=MCS
                     /note="MCS"
                     /note="color: #0080ff"
     primer_bind     1681..1745
                     /label=BamHI-attP39B-GusR
                     /note="color: black; sequence: 
                     TGCGTAATGGATCCccccaactggggtaacctttgagttctctcagttgggggTTTTGA
                     GACACGGGCCAGAG"
     misc_recomb     1687..1725
                     /label=attP39B
                     /note="color: #ff6600; direction: RIGHT"
     protein_bind    1772..1871
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /note="color: #31849b; direction: RIGHT"
     CDS             1901..2578
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     protein_bind    complement(2580..2679)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /note="color: #31849b; direction: LEFT"
     primer_bind     complement(2679..2732)
                     /label=EagI-attB34-GusF
                     /note="color: black; sequence: 
                     ATTGTACGCGGCCGGTGCCAGGGCGTGCCCTTGGGCTCCCCGGGCGCGATTACCGCTAG
                     CTGGATGGC"
     misc_feature    complement(2699..2732)
                     /label=attB34
                     /note="Minimal attB site as determined by Calos Lab"
                     /note="color: #ff0000; direction: LEFT"
     misc_feature    2733..4838
                     /label=Below threshold
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2733..4838
                     /label=Contig 2(1&gt;4774)
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2733..4837
                     /label=Contig 2(1&gt;4774)
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    2733..3622
                     /label=Below threshold
                     /note="color: #625fff; direction: RIGHT"
     misc_feature    2733..2739
                     /label=MCS
                     /note="MCS"
                     /note="color: #0080ff"
     promoter        complement(2740..4730)
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(2750..2773)
                     /label=Ubi47-F
                     /note="Bob Designed Primer"
                     /note="color: black; sequence: GATGCTCACCCTGTTGTTTGGTGT"
     misc_feature    2917..3029
                     /label=DNA_JOIN
                     /note="joined contig Contig 2 with Contig 1"
                     /note="color: #0000ff; direction: RIGHT"
     primer_bind     complement(3047..3072)
                     /label=Ubi1660(F)
                     /note="Ubi1660(F)"
                     /note="color: #50ff50; direction: LEFT"
     primer_bind     complement(3047..3072)
                     /label=Ubi1660F
                     /note="Bob Designed Primer"
                     /note="color: black; sequence: GGAACTGTATGTGTGTGTCATACATC"
     primer_bind     3053..3075
                     /label=Contig4Fwd3
                     /note="TM-59.01"
                     /note="color: black; sequence: TGACACACACATACAGTTCCAAA"
     primer_bind     3151..3170
                     /label=pWY56cUbiR
                     /note="TM-59.97"
                     /note="color: black; sequence: CCACATCATCACAACCAAGC"
     primer_bind     3151..3170
                     /label=pWY56cWalk1
                     /note="TM-59.97 Walk into pWY56c from end of NOS"
                     /note="color: black; sequence: CCACATCATCACAACCAAGC"
     primer_bind     3151..3170
                     /label=Ubi1592R
                     /note="Bob Designed Primer"
                     /note="color: black; sequence: CCACATCATCACAACCAAGC"
     primer_bind     3284..3303
                     /label=Contig4Fwd3
                     /note="TM-59.01"
                     /note="color: black; sequence: TGACACACACATACAGTTCCAAA"
     misc_feature    3382..3511
                     /label=Only_once
                     /note="color: #ffa552; direction: RIGHT"
     primer_bind     complement(3398..3417)
                     /label=P3F1
                     /note="TM-60 Sequence from back half of Ubi"
                     /note="color: black; sequence: CGGGTCATCTTTTCATGCTT"
     primer_bind     complement(3398..3417)
                     /label=UbiIntF1
                     /note="TM-60.07 Ubi Forward Primer designed for making INT 
                     Fish Probe"
                     /note="color: black; sequence: cgggtcatcttttcatgctt"
     primer_bind     complement(3460..3479)
                     /label=IntegraseProbe3F
                     /note="TM-59.9 Second Half Ubiquitin into Integrase"
                     /note="color: black; sequence: cgttgcatagggtttggttt"
     primer_bind     complement(3460..3479)
                     /label=UbiIntF2
                     /note="TM-59.86 Ubi Forward Primer designed for making INT 
                     Fish Probe"
                     /note="color: black; sequence: cgttgcatagggtttggttt"
     primer_bind     3539..3558
                     /label=Contig6Fwd1
                     /note="TM-60.08"
                     /note="color: black; sequence: TCCCCAAAGAGAAACACTGG"
     primer_bind     3539..3558
                     /label=IntegraseProbe2R
                     /note="TM-60.08 First Half of Ubiquitin"
                     /note="color: black; sequence: tccccaaagagaaacactgg"
     primer_bind     3554..3576
                     /label=Contig6Fwd1 (2)
                     /note="TM-59.84"
                     /note="color: black; sequence: ACTGGCAAGTTAGCAATCAGAAC"
     misc_feature    3623..3624
                     /label=Above threshold
                     /note="color: #00ff00; direction: RIGHT"
     misc_feature    3625..4837
                     /label=Below threshold
                     /note="color: #625fff; direction: RIGHT"
     primer_bind     complement(3661..3680)
                     /label=Ubi1063F
                     /note="Bob Designed Primer"
                     /note="color: black; sequence: GGGCCCGGTAGTTCTACTTC"
     misc_feature    3739..3966
                     /label=Only_once
                     /note="color: #ffa552; direction: RIGHT"
     primer_bind     3774..3793
                     /label=IntegraseProbe2R2
                     /note="Tm-60.1 First Half of Ubiquitin"
                     /note="color: black; sequence: gatttgggggagatctggtt"
     misc_feature    3967..4837
                     /label=One_strand
                     /note="color: #ff0000; direction: RIGHT"
     primer_bind     complement(3982..4001)
                     /label=Contig2Rvs1
                     /note="TM-61.57"
                     /note="color: black; sequence: CTGTCGGCATCCAGAAATTG"
     primer_bind     complement(4109..4130)
                     /label=Contig2Rvs2
                     /note="TM-60.96"
                     /note="color: black; sequence: GAGTCTAACGGACACCAACCAG"
     primer_bind     4432..4455
                     /label=UbiPRev1
                     /note="color: black; sequence: AAAAAGGAGAACACATGCACACTA"
     primer_bind     complement(4432..4453)
                     /label=pTFPhiF1
                     /note="TM-59.15 Amplify ubi-int-nos for probe (Not full)"
                     /note="color: black; sequence: gtgtgcatgtgttctccttttt"
     primer_bind     complement(4432..4453)
                     /label=pTFPhiF2
                     /note="TM-59.15 Amplify ubi-int-nos for probe (Not full)"
                     /note="color: black; sequence: gtgtgcatgtgttctccttttt"
     primer_bind     complement(4466..4485)
                     /label=Ubi2-F
                     /note="color: black; sequence: TGACAACAGGACTCTACAGT"
     primer_bind     complement(4620..4641)
                     /label=UbiF2
                     /note="TM-58.48 Primer of UBI before primer begins on GZUS"
                     /note="color: black; sequence: GTCACACTTGTTTGAAGTGCAG"
     primer_bind     complement(4622..4643)
                     /label=Ubi1F1
                     /note="color: black; sequence: TTGTCACACTTGTTTGAAGTGC"
     primer_bind     complement(4622..4643)
                     /label=UbiF1
                     /note="TM-58.88 Primer of UBI before primer begins on GZUS"
                     /note="color: black; sequence: TTGTCACACTTGTTTGAAGTGC"
     primer_bind     complement(4668..4685)
                     /label=IntegraseProbe2F
                     /note="Tm-58.95 First Half of Ubiquitin"
                     /note="color: black; sequence: gcgcattgcatgtctaagtt"
     primer_bind     complement(4680..4700)
                     /label=Ubi1F
                     /note="color: black; sequence: CTCTAGAGATAATGAGCATTG"
     primer_bind     complement(4699..4718)
                     /label=IntExc_fwd
                     /note="TM-62.1 NEBuilder primers for LongampTaq"
                     /note="color: black; sequence: 
                     caggcatgcaagctcaTGACCCGGTCGTGCCCCTCT"
     primer_bind     complement(4714..4731)
                     /label=HIIInoBUbi56GFP-F
                     /note="Amplify Ubi-EGFP-Nos from p56EGFP to insert into 
                     pZY101"
                     /note="color: black; sequence: 
                     GCTTAAAGCTTATTATGCAGTGCAGCGTGACC"
     misc_feature    4716..4837
                     /label=pMECA
                     /note="pMECA"
                     /note="color: #0080ff"
     primer_bind     complement(4721..4733)
                     /label=UbiFullNoHINDIII
                     /note="TM-61.15 Full Ubi No HINDIII"
                     /note="color: black; sequence: GCTTGCATGCCTGCAGTGCAG"
     primer_bind     4742..4760
                     /label=pMECAControl64
                     /note="TM-58 Screen for pMECA insert into pNG2 65bp Product
                     with Screen1, only for use with pWY82-Full"
                     /note="color: black; sequence: gggcgacgtcgactgttta"
     primer_bind     complement(4785..4806)
                     /label=pMECAScreen1
                     /note="TM-60 Screen for pMECA insert into pNG2 100bp to 
                     FSE1"
                     /note="color: black; sequence: gatctagacgtacgtggccaat"
     primer_bind     complement(4795..4813)
                     /label=pMECARvsBsaAI
                     /note="TM-60.13 Primer Rvs in pMECA BsaAI-BssHii"
                     /note="color: black; sequence: cgcgccagatctagacgta"
     primer_bind     complement(4795..4813)
                     /label=pMECAScreen2
                     /note="TM-59.1 Screen for pMECA insert into pNG2 107bp to 
                     FSE1"
                     /note="color: black; sequence: cgcgccagatctagacgta"
     misc_feature    join(4838..5792,1..1685)
                     /label=Below threshold
                     /note="This forward directional feature has 3 segments:
                        1: 4838 ..  260 / #a6acb3
                        2:  261 ..  262 / #------
                        3:  263 .. 1685 / #a6acb3"
     misc_feature    join(4838..5792,1..1685)
                     /label=Contig 2(1&gt;4774)
                     /note="This forward directional feature has 3 segments:
                        1: 4838 ..  260 / #a6acb3
                        2:  261 ..  262 / #------
                        3:  263 .. 1685 / #a6acb3"
     misc_feature    4838..5280
                     /label=One_strand
                     /note="color: #ff0000; direction: RIGHT"
     promoter        complement(4850..4868)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(4889..4910)
                     /label=m13R-23mer
                     /note="color: black; sequence: TCACACAGGAAACAGCTATGAC"
     primer_bind     complement(4889..4905)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(4889..4905)
                     /label=m13R-17mer
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     protein_bind    4913..4929
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     complement(4917..4936)
                     /label=HindIII-AntiGusF
                     /note="color: black; sequence: 
                     ATGAATCAAAGCTTTGTGGAATTGTGAGCGGATA"
     primer_bind     complement(4917..4936)
                     /label=pGEMScreenR
                     /note="TM-60.07 Screen for pGEMT or Easy Inserts 163 to 
                     Insert (Easy)"
                     /note="color: black; sequence: TGTGGAATTGTGAGCGGATA"
     primer_bind     complement(4917..4936)
                     /label=topo2.1ScreenF
                     /note="TM-60.07 Sequence across topo insert 232 without 
                     insert"
                     /note="color: black; sequence: TGTGGAATTGTGAGCGGATA"
     primer_bind     complement(4923..4945)
                     /label=ptf101TeloR
                     /note="TM-60.33 Sequence into Telo"
                     /note="color: black; sequence: CGTATGTTGTGTGGAATTGTGAG"
     primer_bind     complement(4925..4947)
                     /label=ptf101TeloR2
                     /note="TM- 60.33 Sequence into Telo of ptf101.1 and 
                     derivatives from RB into EcoRI site"
                     /note="color: black; sequence: CTCGTATGTTGTGTGGAATTGTG"
     promoter        complement(4937..4967)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 4937 .. 4943 / #ffffff / -10
                        2: 4944 .. 4961 / #ffffff
                        3: 4962 .. 4967 / #ffffff / -35"
     protein_bind    4982..5003
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     primer_bind     complement(5115..5134)
                     /label=pSP72R
                     /note="336"
                     /note="color: black; sequence: GAGTCAGTGAGCGAGGAAGC"
     misc_feature    join(5281..5792,1..262)
                     /label=Only_once
                     /note="color: #ffa552; direction: RIGHT"
     rep_origin      complement(join(5291..5792,1..89))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(5310..5329)
                     /label=pBlueMCSR1
                     /note="TM-59.84 Sequence into MCS of pBluescriptIIKS+"
                     /note="color: black; sequence: TTTTTGTGATGCTCGTCAGG"
ORIGIN
        1 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
       61 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      121 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
      181 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
      241 agtaaaactt ggtctgacag ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
      301 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
      361 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      421 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      481 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      541 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      601 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      661 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      721 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      781 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      841 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      901 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      961 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     1021 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     1081 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     1141 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     1201 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     1261 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1321 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1381 atgagcggat acatatttga atgtatagct tgatatcgaa ttcccgatct agtaacatag
     1441 atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt
     1501 attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat aacgtcatgc
     1561 attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg ataatcatcg
     1621 caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt gaacgatcgg
     1681 ggatcccccc aactggggta acctttgagt tctctcagtt gggggttttg agacacgggc
     1741 cagagctgca gctagcatgg atctcgggcc ccaaataatg attttatttt gactgatagt
     1801 gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa ctttgtacaa
     1861 aaaagcaggc tcatttaact ttaagaagga gatatatacc atggcctcct ccgagaacgt
     1921 catcaccgag ttcatgcgct tcaaggtgcg catggagggc accgtgaacg gccacgagtt
     1981 cgagatcgag ggcgagggcg agggccgccc ctacgagggc cacaacaccg tgaagctgaa
     2041 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttccagta
     2101 cggctccaag gtgtacgtga agcaccccgc cgacatcccc gactacaaga agctgtcctt
     2161 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggcgaccgt
     2221 gacccaggac tcctccctgc aggacggctg cttcatctac aaggtgaagt tcatcggcgt
     2281 gaacttcccc tccgacggcc ccgtgatgca gaagaagacc atgggctggg aggcctccac
     2341 cgagcgcctg tacccccgcg acggcgtgct gaagggcgag acccacaagg ccctgaagct
     2401 gaaggacggc ggccactacc tggtggagtt caagtccatc tacatggcca agaagcccgt
     2461 gcagctgccc ggctactact acgtggacgc caagctggac atcacctccc acaacgagga
     2521 ctacaccatc gtggagcagt acgagcgcac cgagggccgc caccacctgt tcctgtagta
     2581 cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
     2641 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagcta gcggtaatcg
     2701 cgcccgggga gcccaagggc acgccctggc acatttaaat gcagaagtaa caccaaacaa
     2761 cagggtgagc atcgacaaaa gaaacagtac caagcaaata aatagcgtat gaaggcaggg
     2821 ctaaaaaaat ccacatatag ctgctgcata tgccatcatc caagtatatc aagatcaaaa
     2881 taattataaa acatacttgt ttattataat agataggtac tcaaggttag agcatatgaa
     2941 tagatgctgc atatgccatc atgtatatgc atcagtaaaa cccacatcaa catgtatacc
     3001 tatcctagat cgatatttcc atccatctta aactcgtaac tatgaagatg tatgacacac
     3061 acatacagtt ccaaaattaa taaatacacc aggtagtttg aaacagtatt ctactccgat
     3121 ctagaacgaa tgaacgaccg cccaaccaca ccacatcatc acaaccaagc gaacaaaaag
     3181 catctctgta tatgcatcag taaaacccgc atcaacatgt atacctatcc tagatcgata
     3241 tttccatcca tcatcttcaa ttcgtaacta tgaatatgta tggcacacac atacagatcc
     3301 aaaattaata aatccaccag gtagtttgaa acagaattct actccgatct agaacgaccg
     3361 cccaaccaga ccacatcatc acaaccaaga caaaaaaaag catgaaaaga tgacccgaca
     3421 aacaagtgca cggcatatat tgaaataaag gaaaagggca aaccaaaccc tatgcaacga
     3481 aacaaaaaaa atcatgaaat cgatcccgtc tgcggaacgg ctagagccat cccaggattc
     3541 cccaaagaga aacactggca agttagcaat cagaacgtgt ctgacgtaca ggtcgcatcc
     3601 gtgtacgaac gctagcagca cggatctaac acaaacacgg atctaacaca aacatgaaca
     3661 gaagtagaac taccgggccc taaccatgga ccggaacgcc gatctagaga aggtagagag
     3721 gggggggggg ggaggacgag cggcgtacct tgaagcggag gtgccgacgg gtggatttgg
     3781 gggagatctg gttgtgtgtg tgtgcgctcc gaacaacacg aggttgggga aagagggtgt
     3841 ggagggggtg tctatttatt acggcgggcg aggaagggaa agcgaaggag cggtgggaaa
     3901 ggaatccccc gtagctgccg gtgccgtgag aggaggagga ggccgcctgc cgtgccggct
     3961 cacgtctgcc gctccgccac gcaatttctg gatgccgaca gcggagcaag tccaacggtg
     4021 gagcggaact ctcgagaggg gtccagaggc agcgacagag atgccgtgcc gtctgcttcg
     4081 cttggcccga cgcgacgctg ctggttcgct ggttggtgtc cgttagactc gtcgacggcg
     4141 tttaacaggc tggcattatc tactcgaaac aagaaaaatg tttccttagt ttttttaatt
     4201 tcttaaaggg tatttgttta atttttagtc actttatttt attctatttt atatctaaat
     4261 tattaaataa aaaaactaaa atagagtttt agttttctta atttagaggc taaaatagaa
     4321 taaaatagat gtactaaaaa aattagtcta taaaaaccat taaccctaaa ccctaaatgg
     4381 atgtactaat aaaatggatg aagtattata taggtgaagc tatttgcaaa aaaaaaggag
     4441 aacacatgca cactaaaaag ataaaactgt agagtcctgt tgtcaaaata ctcaattgtc
     4501 ctttagacca tgtctaactg ttcatttata tgattctcta aaacactgat attattgtag
     4561 tactatagat tatattattc gtagagtaaa gtttaaatat atgtataaag atagataaac
     4621 tgcacttcaa acaagtgtga caaaaaaaat atgtggtaat tttttataac ttagacatgc
     4681 aatgctcatt atctctagag aggggcacga ccgggtcacg ctgcactgca tgctgcagcc
     4741 cgggcgacgt cgactgttta aacgaggcct taattaaggg cccaattggc cacgtacgtc
     4801 tagatctggc gcgcctcgag gatcgatcca tggtacccag cttttgttcc ctttagtgag
     4861 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     4921 cgctcacaat tccacacaac atacgagccg ggagcataaa gtgtaaagcc tggggtgcct
     4981 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     5041 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     5101 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     5161 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     5221 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     5281 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     5341 gtcagaggtg gcgaaaaccc gacaggacta taaagatacc aggcgttttc cccctggaag
     5401 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     5461 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     5521 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     5581 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     5641 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     5701 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     5761 gaagccagtt accttcggaa aaagagttgg ta
//
