LOCUS       Exported               11209 bp ds-DNA     circular SYN 19-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11209)
  AUTHORS   Adarsh
  TITLE     Direct Submission
  JOURNAL   Exported Jul 19, 2020 from SnapGene Viewer 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11209
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        245..574
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      425..560
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        582..961
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        963..1161
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             1179..1359
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1406..1531
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2028..2261
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2788..2905
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3042..3449
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3510..5075
                     /codon_start=1
                     /label=hNup62
                     /translation="MSGFNFGGTGAPTGGFTFGTAKTATTTPATGFSFSTSGTGGFNFG
                     APFQPATSTPSTGLFSLATQTPATQTTGFTFGTATLASGGTGFSLGIGASKLNLSNTAA
                     TPAMANPSGFGLGSSNLTNAISSTVTSSQGTAPTGFVFGPSTTSVAPATTSGGFSFTGG
                     STAQPSGFNIGSAGNSAQPTAPATLPFTPATPAATTAGATQPAAPTPTATITSTGPSLF
                     ASIATAPTSSATTGLSLCTPVTTAGAPTAGTQGFSLKAPGAASGTSTTTSTAATATATT
                     TTSSSTTGFALNLKPLAPAGIPSNTAAAVTAPPGPGAAAGAAASSAMTYAQLESLINKW
                     SLELEDQERHFLQQATQVNAWDRTLIENGEKITSLHREVEKVKLDQKRLDQELDFILSQ
                     QKELEDLLSPLEELVKEQSGTIYLQHADEEREKTYKLAENIDAQLKRMAQDLKDIIEHL
                     NTSGAPADTSDPLQQICKILNAHMDSLQWIDQNSALLQRKVEEVTKVCEGRRKEQERSF
                     RITFD"
     CDS             5082..5402
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     regulatory      5409..5418
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             5415..6128
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     CDS             6132..6851
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    6912..7485
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             7491..8090
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    8100..8688
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(8571..8582)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9206..9386
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(9457..10045)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10216..11076)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11077..11181)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgctgtg
      241 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
      301 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
      361 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
      421 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
      481 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
      541 aggaggcttt tttggaggcc taggcttttg caaaaagctt tgacattgat tattgactag
      601 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      661 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      721 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      781 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      841 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      901 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      961 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     1021 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     1081 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     1141 gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga
     1201 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     1261 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     1321 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     1381 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     1441 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     1501 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     1561 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     1621 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     1681 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     1741 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     1801 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     1861 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     1921 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     1981 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     2041 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     2101 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     2161 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     2221 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     2281 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     2341 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     2401 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     2461 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     2521 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     2581 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     2641 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     2701 agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc gccaattctg
     2761 cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     2821 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     2881 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
     2941 gttaattaac tgcagccccg ataaaataaa agattttatt tagtctccag aaaaaggggg
     3001 gaatgaaaga ccccacctgt aggtttggca agctagctgc agtaacgcca ttttgcaagg
     3061 catggaaaaa taccaaacca agaatagaga agttcagatc aagggcgggt acatgaaaat
     3121 agctaacgtt gggccaaaca ggatatctgc ggtgagcagt ttcggccccg gcccggggcc
     3181 aagaacagat ggtcaccgca gtttcggccc cggcccgagg ccaagaacag atggtcccca
     3241 gatatggccc aaccctcagc agtttcttaa gacccatcag atgtttccag gctcccccaa
     3301 ggacctgaaa tgaccctgcg ccttatttga attaaccaat cagcctgctt ctcgcttctg
     3361 ttcgcgcgct tctgcttccc gagctctata aaagagctca caacccctca ctcggcgcgc
     3421 cagtcctccg acagactgag tcgcccgggg gtctagaagc gctggatccg tttaaacgcg
     3481 gccgcccagc acagtggctc gaggccacca tgagcgggtt taattttgga ggcactgggg
     3541 cccctacagg cgggttcacg tttggcactg caaagacggc aacaaccaca cctgctacag
     3601 ggttttcttt ctccacctct ggcactggag ggtttaattt tggggctccc ttccaaccag
     3661 ccacaagtac cccttccacc ggcctgttct cacttgccac ccagactccg gccacacaga
     3721 cgacaggctt cacttttgga acagcgactc ttgcttcggg gggaactgga ttttctttgg
     3781 ggatcggtgc ttcaaagctc aacttgagca acacagctgc caccccagcc atggcaaacc
     3841 ccagcggctt tgggctgggc agcagcaacc tcactaatgc catatcgagc accgtcacct
     3901 ccagccaggg cacagcaccc accggctttg tgtttggccc ctccaccacc tctgtggctc
     3961 cagctaccac atctggaggc ttctcattca ctggtggaag cacggcccaa ccctccggtt
     4021 tcaacattgg ctcagcaggg aattcagccc agcccacggc acctgccacg ttgcccttca
     4081 ctccggccac gccagcagcc accacagcag gtgccacaca gccagctgct cccacaccca
     4141 cagccaccat caccagtact gggcccagcc tctttgcgtc aatagcaact gctccaacct
     4201 catctgccac cactggactc tccctctgta cccctgtgac cacagcgggc gcccccactg
     4261 ctgggacaca gggcttcagc ttaaaggcac ctggagcagc ttccggcacc tccacaacaa
     4321 catccaccgc tgccaccgcc accgccacca ccaccaccag cagcagcacc accggctttg
     4381 ccttgaattt aaaaccactg gcgccagccg ggatccccag caatacagca gctgccgtga
     4441 ccgctccacc tggccctggc gcagctgcag gggcggctgc cagctccgcc atgacctacg
     4501 cgcagctgga gagcctgatc aacaaatgga gcctggagct agaggaccag gagcggcact
     4561 tcctccagca ggccacccag gtcaacgcct gggaccgcac gctgatcgag aatggagaaa
     4621 agatcaccag cctgcaccgc gaggtggaga aggtgaagct ggaccagaag aggctggacc
     4681 aggagctcga cttcatcctg tcccagcaga aggagctgga agacctgctg agcccactgg
     4741 aggagttggt caaggagcag agcgggacca tctacctgca gcacgcggat gaggagcgtg
     4801 agaaaaccta caagctggct gagaacatcg acgcacagct caagcgcatg gcccaggatc
     4861 tcaaggacat catcgagcac ctgaacacgt ccggggcccc cgccgacacc agtgacccac
     4921 tgcagcagat ctgcaagatc ctcaatgcgc acatggactc actgcagtgg atcgaccaga
     4981 actcggccct gctgcagagg aaggtggagg aggtgaccaa ggtgtgcgag ggccggcgca
     5041 aggagcagga gcgcagcttc cggatcacct ttgacacgcg tggagtgcag gtggagacta
     5101 tctccccagg agacgggcgc accttcccca agcgcggcca gacctgcgtg gtgcactaca
     5161 ccgggatgct tgaagatgga aagaaagttg attcctcccg ggacagaaac aagcccttta
     5221 agtttatgct aggcaagcag gaggtgatcc gaggctggga agaaggggtt gcccagatga
     5281 gtgtgggtca gagagccaaa ctgactatat ctccagatta tgcctatggt gccactgggc
     5341 acccaggcat catcccacca catgccactc tcgtcttcga tgtggagctc ctaaaactgg
     5401 aaccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     5461 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     5521 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     5581 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     5641 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     5701 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     5761 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     5821 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     5881 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     5941 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     6001 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     6061 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     6121 agctgtacgg aatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     6181 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     6241 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     6301 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     6361 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     6421 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     6481 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     6541 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     6601 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     6661 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     6721 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     6781 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     6841 tgtacaagta aagcggccgc gactttagat cattcgttaa ctggccagaa tttcgccccc
     6901 cccccccccc ccccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
     6961 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     7021 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     7081 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     7141 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     7201 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     7261 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     7321 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     7381 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     7441 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataaccggt atgaccgagt
     7501 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     7561 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     7621 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     7681 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     7741 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     7801 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     7861 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     7921 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     7981 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     8041 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga ttaatcgcta
     8101 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     8161 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     8221 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     8281 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     8341 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     8401 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     8461 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     8521 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     8581 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     8641 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgctt aatcgcgtcg
     8701 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     8761 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     8821 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     8881 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     8941 ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata
     9001 tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga
     9061 agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga
     9121 cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc
     9181 ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct gagcctggga
     9241 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     9301 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     9361 ttagtcagtg tggaaaatct ctagcagggc ccgtttcatg tgagcaaaag gccagcaaaa
     9421 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9481 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9541 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9601 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9661 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9721 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9781 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9841 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     9901 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9961 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    10021 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    10081 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    10141 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    10201 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    10261 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    10321 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    10381 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    10441 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10501 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10561 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10621 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10681 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10741 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10801 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10861 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10921 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10981 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    11041 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    11101 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    11161 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
