LOCUS       P526                    3300 bp ds-DNA     circular     21-MAY-2020
DEFINITION  .
ACCESSION   urn.local...md-5xoajnh                                             
VERSION     urn.local...md-5xoajnh                                             
COMMENT     
COMMENT     Module B
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    537..537
                     /locus_tag="BsaI overhang"
                     /ApEinfo_label="BsaI overhang"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#cc66ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     Promoter        24..450
                     /locus_tag="CaMV 35S promoter short"
                     /ApEinfo_label="CaMV 35S promoter short"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     5'_UTR          467..528
                     /locus_tag="TMV Omega"
                     /ApEinfo_label="TMV Omega"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      join(2446..3300,1..2)
                     /locus_tag="High Copy Ori"
                     /ApEinfo_label="High Copy Ori"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            1325..2378
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1057..1060
                     /locus_tag="BsaI overhang(1)"
                     /ApEinfo_label="BsaI overhang"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#cc66ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1026..1044)
                     /locus_tag="oCS2166"
                     /ApEinfo_label="oCS2166"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     Terminator      1061..1276
                     /locus_tag="g7 terminator"
                     /ApEinfo_label="g7 terminator"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    605..625
                     /locus_tag="oCS2165"
                     /ApEinfo_label="oCS2165"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    538..1056
                     /locus_tag="hpaC"
                     /ApEinfo_label="hpaC"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2173..2178
                     /locus_tag="BsaI Binding site"
                     /ApEinfo_label="BsaI Binding site"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="#333333"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1301..1307
                     /locus_tag="AarI site (N4v top strand; N8v bottom strand)"
                     /ApEinfo_label="AarI site (N4v top strand; N8v bottom
                     strand)"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ffcc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1..7)
                     /locus_tag="AarI site (N4v top strand; N8v bottom
                     strand)(1)"
                     /ApEinfo_label="AarI site (N4v top strand; N8v bottom
                     strand)"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ffcc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(3291..3297)
                     /locus_tag="SapI site"
                     /ApEinfo_label="SapI site"
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    12..15
                     /locus_tag="AarI overhang"
                     /ApEinfo_label="AarI overhang"
                     /ApEinfo_fwdcolor="#cc66fe"
                     /ApEinfo_revcolor="#cc66fe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1293..1296
                     /locus_tag="AarI overhang(1)"
                     /ApEinfo_label="AarI overhang"
                     /ApEinfo_fwdcolor="#cc66fe"
                     /ApEinfo_revcolor="#cc66fe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 cacctgctca tGGACggcgc gccGTCAACA TGGTGGAGCA CGACACTCTG GTCTACTCCA
       61 AAAATGTCAA AGATACAGTC wTCAGAAGAT CAAAGGGCTA TTGAGACTTT TCAACAAAGG
      121 ATAATTTCGG GAAACCTCCT CGGATTCCAT TGCCCAGCTA TCTGTCACTT CATCGAAAGG
      181 ACAGTAGAAA AGGAAGGTGG CTCCTACAAA TGCCATCATT GCGATAAAGG AAAGGCTATC
      241 ATTCAAGATC TCTCTGCCGA CAGTGGTCCC AAAGATGGAC CCCCACCCAC GAGGAGCATC
      301 GTGGAAAAAG AAGAGGTTCC AACCACGTCT ACAAAGCAAG TGGATTGATG TGACATCTCC
      361 ACTGACGTAA GGGATGACGC ACAATCCCAC TATCCTTCGC AAGACCCTTC CTCTATATAA
      421 GGAAGTTCAT TTCATTTGGA GAGGACACGC TCGAGTATAA GAGCTCATTT TTACAACAAT
      481 TACCAACAAC AACAAACAAC AAACAACATT ACAATTACAT TTACAATTAT CGATACAATG
      541 CAATTAGACG AGCAGCGTCT TAGATTTCGT GATGCTATGG CTTCTCTTTC TGCCGCTGTT
      601 AATATCATTA CAACGGAAGG CGACGCAGGT CAATGCGGCA TCACTGCCAC TGCTGTCTGC
      661 AGCGTCACGG ACACGCCACC GTCTTTGATG GTTTGCATAA ATGCCAATTC AGCAATGAAC
      721 CCTGTGTTCC AAGGTAACGG CAAGCTCTGT GTAAATGTTC TCAATCACGA GCAAGAGTTA
      781 ATGGCACGTC ACTTTGCAGG GATGACCGGG ATGGCAATGG AGGAACGTTT TTCCCTCTCT
      841 TGCTGGCAAA AAGGCCCGCT GGCTCAACCT GTACTGAAAG GATCACTCGC CTCCCTTGAG
      901 GGGGAAATCA GAGAtGTGCA GGCTATTGGC ACACACCTCG TGTACCTCGT TGAGATTAAG
      961 AACATAATTT TAAGCGCAGA GGGCCACGGG CTGATCTACT TTAAGAGGCG ATTTCATCCA
     1021 GTTATGCTGG AAATGGAGGC TGCAATCAGT GGCTGAGCTT GATCCCCCGT CGACAGCTAG
     1081 CTATATCATC AATTTATGTA TTACACATAA TATCGCACTC AGTCTTTCAT CTACGGCAAT
     1141 GTACCAGCTG ATATAATCAG TTATTGAAAT ATTTCTGAAT TTAAACTTGC ATCAATAAAT
     1201 TTATGTTTTT GCTTGGACTA TAATACCTGA CTTGTTATTT TATCAATAAA TATTTAAACT
     1261 ATATTTCTTT CAAGATccag agctctggtg acCCGGgtgg gcaggtgtga tgaaaagcct
     1321 tagggtcagg tggcactttt cggggaaatg tgcgatgaac ccctatttgt ttatttttct
     1381 aaatacattc aaatatgtat ccgctaatga gacaataacc ctgataaatg cttcaattat
     1441 attgaaaaag gaagagtatg agtatccaac atttccgtgt cgcccttatt cccttttttg
     1501 cggccttctg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     1561 aagatcagtt gggtgcccga gtgggttaca ttgaactgga tctcaacagc ggcaaaatct
     1621 tagagagttt tcgccccgaa gaacgcttcc caatgatgag cactttcaaa gttctgctat
     1681 gtggcgcggt gttatcccgt attgatgctg ggcaagagca actcggtcgc cgcatacact
     1741 attctcagaa tgacttggtt gagtattcac cagtcacaga aaagcatctt acggatggca
     1801 tgacagtaag agaattatgt agtgctgcca taaccatgag tgataacact gcggccaact
     1861 tacttctgac aacgattgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     1921 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     1981 agcgtgacac cacgatgcct gtagcaatgg caacaactct ccgcaaacta ttaactggcg
     2041 aactacttac tcttgcttca cgccagcaac tcattgactg gatggaggcg gacaaagttg
     2101 caggaccact tctgcgctcg gcacttccgg ctggctggtt tattgctgat aaatctggag
     2161 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     2221 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     2281 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagacgaa gggaaataaa
     2341 tagtagcccg cctgatgtgc gggctttttt ttggtggtgg ttaccagtgt cgcttagcta
     2401 ccgtaggtcc tcttaggcgg tccgattagc gacatagtca tcaatgcctt gacggcaaaa
     2461 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2521 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2581 taccagcggt ggtttgtttg ccggatcaag agctactaac tctttttccg aaggtaactg
     2641 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2701 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2761 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2821 ataaggcgca gcggtcgggc tgaacggggg gttcgtgctc acagcccagc ttggagcgaa
     2881 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2941 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3001 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3061 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3121 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3181 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3241 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
//
